Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 726,087 G→T 87.4% R350L (CGT→CTT)  rhsC → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273726,0870GT87.4% 336.8 / 49.3 143R350L (CGT→CTT) rhsCRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base G (12/6);  new base T (71/54);  total (83/60)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 143 total aligned reads displayed.

ACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTC  >  CP009273/725943‑726232
                                                                                                                                                |                                                                                                                                                 
aCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGCTGGTGGCGCACCGTca                                                                                                                                                >  1:15789/1‑148 (MQ=255)
aCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTca                                                                                                                                                >  2:1855975/1‑148 (MQ=255)
aCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTca                                                                                                                                                >  2:1855933/1‑148 (MQ=255)
  ccGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTcaca                                                                                                                                              <  2:124065/148‑1 (MQ=255)
        aaTTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGcc                                                                                                                                        >  1:3183460/1‑148 (MQ=255)
             aCCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCg                                                                                                                                   >  1:3000677/1‑148 (MQ=255)
                    gcgccgcTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCt                                                                                                                            >  1:1027944/1‑148 (MQ=255)
                           tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                     >  1:158650/1‑148 (MQ=255)
                           tGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCg                                                                                                                     >  1:158628/1‑148 (MQ=255)
                            ggTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGt                                                                                                                    <  1:2461190/148‑1 (MQ=255)
                              tGCGCTATGTCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTa                                                                                                                  >  2:2900596/1‑148 (MQ=255)
                                cgcTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACg                                                                                                                <  1:3303716/148‑1 (MQ=255)
                                cgcTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACg                                                                                                                <  2:31167/148‑1 (MQ=255)
                                cgcTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACg                                                                                                                <  2:1705379/148‑1 (MQ=255)
                                  cTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGAc                                                                                                              >  2:1209356/1‑148 (MQ=255)
                                   tATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACa                                                                                                             >  1:1296792/1‑148 (MQ=255)
                                     tGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGc                                                                                                           >  1:10255/1‑148 (MQ=255)
                                         tGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcg                                                                                                       <  2:1239419/148‑1 (MQ=255)
                                           gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                     >  1:1636572/1‑148 (MQ=255)
                                           gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                     >  1:2997715/1‑148 (MQ=255)
                                           gACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                     <  2:2636809/148‑1 (MQ=255)
                                           gACGCCCCGCGGCGAACTGGCGGCGGGGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcggg                                                                                                     <  2:2636797/148‑1 (MQ=255)
                                              gCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcgg                                                                                                  <  2:2938786/148‑1 (MQ=255)
                                              gCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcgg                                                                                                  <  2:2938725/148‑1 (MQ=255)
                                              gCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcgg                                                                                                  <  2:22106/148‑1 (MQ=255)
                                               ccccGCGGCGAACTGGCGGCGGTGTATGATCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGAcgggcggg                                                                                                 <  2:1372431/148‑1 (MQ=255)
                                                cccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGt                                                                                                <  2:967304/148‑1 (MQ=255)
                                                 ccGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTg                                                                                               >  1:351804/1‑148 (MQ=255)
                                                       cgAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGaa                                                                                         >  2:2820076/1‑148 (MQ=255)
                                                       cgAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGaa                                                                                         >  2:2820110/1‑148 (MQ=255)
                                                         aaCTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACa                                                                                       >  1:2009493/1‑148 (MQ=255)
                                                           cTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGc                                                                                     >  1:285536/1‑148 (MQ=255)
                                                           cTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGc                                                                                     >  1:286062/1‑148 (MQ=255)
                                                              gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACCCGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                  <  2:1056113/148‑1 (MQ=255)
                                                              gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                  <  2:1507529/148‑1 (MQ=255)
                                                              gcggcgGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaa                                                                                  >  2:854032/1‑148 (MQ=255)
                                                               cggcggTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTaaa                                                                                 <  1:2306478/148‑1 (MQ=255)
                                                                        aTGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAgg                                                                        >  1:2410130/1‑148 (MQ=255)
                                                                        aTGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAgg                                                                        >  1:2410124/1‑148 (MQ=255)
                                                                        aTGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAgg                                                                        >  1:2410118/1‑148 (MQ=255)
                                                                        aTGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAgg                                                                        <  1:1034569/148‑1 (MQ=255)
                                                                         tGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGc                                                                       >  2:694894/1‑148 (MQ=255)
                                                                          gACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCt                                                                      <  2:938058/148‑1 (MQ=255)
                                                                           aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCtt                                                                     <  2:2169317/148‑1 (MQ=255)
                                                                            ccGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTa                                                                    <  1:641048/148‑1 (MQ=255)
                                                                            ccGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTa                                                                    <  2:1027944/148‑1 (MQ=255)
                                                                            ccGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTa                                                                    <  1:2300486/148‑1 (MQ=255)
                                                                              gcagcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAg                                                                  <  1:122927/148‑1 (MQ=255)
                                                                              gcagcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAg                                                                  <  2:634978/148‑1 (MQ=255)
                                                                              gcagcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAg                                                                  <  1:1795230/148‑1 (MQ=255)
                                                                                  caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                              >  2:1251317/1‑148 (MQ=255)
                                                                                  caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                              >  2:1251273/1‑148 (MQ=255)
                                                                                  caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                              <  1:1749260/148‑1 (MQ=255)
                                                                                  caATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTac                                                              <  1:2489587/148‑1 (MQ=255)
                                                                                    aTACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTacac                                                            >  1:2822602/1‑148 (MQ=255)
                                                                                       cacaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTa                                                         >  1:3191745/1‑148 (MQ=38)
                                                                                       cacaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTa                                                         >  1:2598460/1‑148 (MQ=38)
                                                                                         caGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATc                                                       <  2:2431648/148‑1 (MQ=37)
                                                                                            gTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGt                                                    <  1:208055/148‑1 (MQ=37)
                                                                                                gcAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATga                                                <  1:52441/148‑1 (MQ=37)
                                                                                                   gCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGaa                                             <  1:1448923/148‑1 (MQ=37)
                                                                                                   gCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGaa                                             <  1:1300922/148‑1 (MQ=37)
                                                                                                     tttACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAgg                                           >  2:2600295/1‑148 (MQ=38)
                                                                                                     tttACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAgg                                           >  2:1318628/1‑148 (MQ=38)
                                                                                                     tttACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAgg                                           >  2:1318632/1‑148 (MQ=38)
                                                                                                     tttACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAgg                                           >  2:669762/1‑148 (MQ=38)
                                                                                                      ttacttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGa                                          >  2:1739903/1‑148 (MQ=255)
                                                                                                      tgacttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGa                                          <  2:2901181/146‑1 (MQ=255)
                                                                                                       tacttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGAc                                         <  1:2556783/148‑1 (MQ=255)
                                                                                                         cttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCg                                       >  1:1352551/1‑148 (MQ=255)
                                                                                                         cttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCg                                       >  1:1352494/1‑148 (MQ=255)
                                                                                                            acGATGATAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcat                                    <  2:1197990/148‑1 (MQ=255)
                                                                                                               atgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                                 >  2:36780/1‑148 (MQ=255)
                                                                                                               atgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcac                                 >  2:988162/1‑148 (MQ=255)
                                                                                                                tgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                                >  1:3033377/1‑148 (MQ=255)
                                                                                                                tgatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                                >  1:389439/1‑148 (MQ=255)
                                                                                                                tgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                                >  1:2436819/1‑148 (MQ=39)
                                                                                                                tgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacc                                >  1:3291603/1‑148 (MQ=39)
                                                                                                                 gatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacca                               <  2:932313/148‑1 (MQ=255)
                                                                                                                 gatAAATACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcacca                               <  2:10255/148‑1 (MQ=255)
                                                                                                                    aaaTACCGGGGCCGGATGGTGTCGCACCTTCACACGGGCCGACCGTAATTCTTTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGTCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatca                            <  1:1209356/148‑1 (MQ=14)
                                                                                                                    aaaTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatca                            <  1:525159/148‑1 (MQ=255)
                                                                                                                    aaaTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatca                            <  1:2877373/148‑1 (MQ=255)
                                                                                                                    aaaTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatca                            >  1:753316/1‑148 (MQ=255)
                                                                                                                    aaaTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatca                            >  2:2059836/1‑148 (MQ=255)
                                                                                                                     aaTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGcatcaccatcac                           >  2:21275/1‑148 (MQ=255)
                                                                                                                      aTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCatcaccatcacc                          >  2:2750966/1‑148 (MQ=255)
                                                                                                                      aTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCatcaccatcacc                          >  1:916200/1‑148 (MQ=255)
                                                                                                                      aTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCatcaccatcacc                          >  1:2611321/1‑148 (MQ=255)
                                                                                                                      aTACCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCatcaccatcacc                          >  2:1942961/1‑148 (MQ=255)
                                                                                                                        aCCGGGGCCGGATGGTGGCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGa                        >  1:611848/1‑148 (MQ=255)
                                                                                                                         ccGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCCTCACCGAc                       <  2:15789/148‑1 (MQ=255)
                                                                                                                                 ggATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGaa               >  2:2438703/1‑148 (MQ=40)
                                                                                                                                   aTGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAcc             >  1:1402616/1‑148 (MQ=255)
                                                                                                                                    tggtggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAccg            <  1:700731/148‑1 (MQ=255)
                                                                                                                                     ggtggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAccgc           >  2:3233201/1‑148 (MQ=255)
                                                                                                                                       tggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAccgccg         >  1:866431/1‑148 (MQ=255)
                                                                                                                                       tggCGCACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAAccgccg         >  1:2583135/1‑148 (MQ=255)
                                                                                                                                         gcgcACCTTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTg       >  1:819947/1‑148 (MQ=255)
                                                                                                                                              ccGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTc  >  1:523370/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTC  >  CP009273/725943‑726232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑