Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 10,444 G→T 100% F17L (TTC→TTA)  satP ← succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927310,4440GT99.6% 770.8 / ‑4.3 232F17L (TTC→TTA) satPsuccinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (110/121);  total (111/121)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

CCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGG  >  CP009273/10301‑10587
                                                                                                                                               |                                                                                                                                               
ccGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATTCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                             >  1:527786/1‑148 (MQ=255)
  gAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGccc                                                                                                                                           >  1:525390/1‑148 (MQ=255)
  gAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGccc                                                                                                                                           >  1:18412/1‑148 (MQ=255)
    aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                         <  1:2542210/148‑1 (MQ=255)
    aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                         <  2:665938/148‑1 (MQ=255)
     aGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATc                                                                                                                                        >  1:1983902/1‑148 (MQ=255)
     aGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATc                                                                                                                                        >  1:1889755/1‑148 (MQ=255)
      gtgtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCa                                                                                                                                       >  2:3098254/1‑148 (MQ=255)
          tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                   >  2:1845123/1‑148 (MQ=255)
          tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                   >  2:2478621/1‑148 (MQ=255)
           gCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGccc                                                                                                                                  <  1:2736759/148‑1 (MQ=255)
               tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                              >  2:2642431/1‑148 (MQ=255)
               tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                              >  2:476236/1‑148 (MQ=255)
               tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                              >  1:1087554/1‑148 (MQ=255)
                ttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAACAGGCCCAGCgg                                                                                                                             >  2:287551/1‑148 (MQ=255)
                  ttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTg                                                                                                                           <  1:2263805/148‑1 (MQ=255)
                     tACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                        >  2:140137/1‑148 (MQ=255)
                        tCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAt                                                                                                                     <  1:3096533/148‑1 (MQ=255)
                         ccAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAtt                                                                                                                    <  1:85978/148‑1 (MQ=255)
                            gcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGc                                                                                                                 >  2:580586/1‑148 (MQ=255)
                             cagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGcc                                                                                                                >  1:1406663/1‑148 (MQ=255)
                              agACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCa                                                                                                               <  2:2317207/148‑1 (MQ=255)
                               gACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCaa                                                                                                              >  2:1824664/1‑148 (MQ=255)
                                aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                             >  2:629980/1‑148 (MQ=255)
                                aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                             >  2:630434/1‑148 (MQ=255)
                                aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                             >  1:371803/1‑148 (MQ=255)
                                    gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCANGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                         >  1:1678849/1‑148 (MQ=255)
                                    gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                         >  2:1126900/1‑148 (MQ=255)
                                     cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgt                                                                                                        >  1:2131528/1‑148 (MQ=255)
                                     cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgt                                                                                                        >  1:601486/1‑148 (MQ=255)
                                      aaaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                                                                                                       <  1:2364518/148‑1 (MQ=255)
                                       aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtgt                                                                                                      <  2:2147628/148‑1 (MQ=255)
                                          aTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGc                                                                                                   >  1:1061735/1‑148 (MQ=255)
                                           tttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCTGATTAGCCAACTTAGTGTTGcc                                                                                                  <  2:2371023/148‑1 (MQ=255)
                                            ttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGccc                                                                                                 <  2:2289848/148‑1 (MQ=255)
                                            ttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGACAACTTAGTGTTGccc                                                                                                 >  2:2003050/1‑148 (MQ=255)
                                                  gATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAtt                                                                                           <  2:1037144/148‑1 (MQ=255)
                                                  gATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAtt                                                                                           <  1:276637/148‑1 (MQ=255)
                                                     gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCt                                                                                        <  1:2993077/148‑1 (MQ=255)
                                                          cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaa                                                                                   >  1:831703/1‑148 (MQ=255)
                                                          cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaa                                                                                   <  2:2425169/148‑1 (MQ=255)
                                                            tAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAat                                                                                 >  2:464782/1‑148 (MQ=255)
                                                             aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                <  2:273329/148‑1 (MQ=255)
                                                             aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                >  2:2508720/1‑148 (MQ=255)
                                                              gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatca                                                                               >  2:1750522/1‑148 (MQ=255)
                                                              gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatca                                                                               <  1:476236/148‑1 (MQ=255)
                                                              gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatca                                                                               >  1:3297732/1‑148 (MQ=255)
                                                                 aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                            <  2:807354/148‑1 (MQ=255)
                                                                  aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                           <  2:2824783/148‑1 (MQ=255)
                                                                   tGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                          >  2:3230098/1‑148 (MQ=255)
                                                                     cccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCga                                                                        >  2:521498/1‑148 (MQ=255)
                                                                     cccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTAGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCga                                                                        >  2:1964653/1‑148 (MQ=255)
                                                                         tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                    <  1:1550103/148‑1 (MQ=255)
                                                                         tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATAATCgaatga                                                                    <  1:2691648/148‑1 (MQ=255)
                                                                         tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                    >  1:1949989/1‑148 (MQ=255)
                                                                          ggCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaa                                                                   >  2:2482454/1‑148 (MQ=255)
                                                                           gCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                  <  1:1229892/148‑1 (MQ=255)
                                                                            cAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGaatgaatg                                                                 <  1:629832/148‑1 (MQ=255)
                                                                                aataatACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGa                                                             >  1:2461822/1‑148 (MQ=255)
                                                                                aataatACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGa                                                             <  2:1231182/148‑1 (MQ=255)
                                                                                aataatACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGa                                                             <  2:1230978/148‑1 (MQ=255)
                                                                                  taataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaaa                                                           <  1:2501656/148‑1 (MQ=255)
                                                                                   aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCTGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                  >  2:2773291/1‑140 (MQ=255)
                                                                                   aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                  <  1:2773291/140‑1 (MQ=255)
                                                                                   aataCAGTCCAGAGCGAAATAACCCACGTTGTGCAGGTAAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGACCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATTGTGAaat                                                          >  1:640562/1‑148 (MQ=255)
                                                                                    ataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaata                                                         >  1:1120258/1‑148 (MQ=255)
                                                                                    ataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaata                                                         <  2:2547752/148‑1 (MQ=255)
                                                                                     taCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataa                                                        >  1:2193060/1‑148 (MQ=255)
                                                                                       ccGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAtt                                                      >  2:2034544/1‑148 (MQ=255)
                                                                                         gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTc                                                    <  1:492550/148‑1 (MQ=255)
                                                                                         gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTc                                                    <  1:920429/148‑1 (MQ=255)
                                                                                           ccAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTccc                                                  <  1:224649/148‑1 (MQ=255)
                                                                                             agagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTg                                                <  2:3140977/148‑1 (MQ=255)
                                                                                              gagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                               >  2:152284/1‑148 (MQ=255)
                                                                                              gagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                               >  2:2183416/1‑148 (MQ=255)
                                                                                                gCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAAt                                             >  2:3066005/1‑148 (MQ=255)
                                                                                                    aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                         >  2:1163231/1‑148 (MQ=255)
                                                                                                    aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                         >  1:1454960/1‑148 (MQ=255)
                                                                                                      tAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCNAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGt                                       <  1:1678006/148‑1 (MQ=255)
                                                                                                        aCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAg                                     <  1:629995/148‑1 (MQ=255)
                                                                                                            aCGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtt                                 >  1:2725347/1‑148 (MQ=255)
                                                                                                             cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGttt                                <  1:932274/148‑1 (MQ=255)
                                                                                                             cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGttt                                <  2:1561186/148‑1 (MQ=255)
                                                                                                             cGTTGTGCAGGTAAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCCTCGAATGAATGGTGAAATAATTTCACTGAAAAACTGTAGTGttt                                >  2:1642961/1‑148 (MQ=255)
                                                                                                                tgtgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCa                             <  1:1774851/148‑1 (MQ=255)
                                                                                                                     aGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGgcg                        >  1:1562256/1‑148 (MQ=255)
                                                                                                                       gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGcgcg                      >  2:3286097/1‑148 (MQ=255)
                                                                                                                       gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGcgcg                      >  1:1749246/1‑148 (MQ=255)
                                                                                                                               gAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataata              <  1:2858732/148‑1 (MQ=255)
                                                                                                                                aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa             >  2:3269738/1‑148 (MQ=255)
                                                                                                                                aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCAtaataa             <  1:1917602/148‑1 (MQ=255)
                                                                                                                                  tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc           <  2:833526/148‑1 (MQ=255)
                                                                                                                                  tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc           <  1:2368128/148‑1 (MQ=255)
                                                                                                                                  tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc           <  2:1855006/148‑1 (MQ=255)
                                                                                                                                    gtggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAg         <  2:718230/148‑1 (MQ=255)
                                                                                                                                    gtggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAg         <  2:1454960/148‑1 (MQ=255)
                                                                                                                                     tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGc        <  2:530009/148‑1 (MQ=255)
                                                                                                                                     tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGc        >  1:1411102/1‑148 (MQ=255)
                                                                                                                                     tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGc        <  2:1948861/148‑1 (MQ=255)
                                                                                                                                           tGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTgg  <  2:371803/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGG  >  CP009273/10301‑10587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑