Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 794,435 G→T 12.2% A132S (GCC→TCC)  pgl → 6‑phosphogluconolactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273794,4350GT12.2% 500.7 / 32.8 152A132S (GCC→TCC) pgl6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base G (69/64);  new base T (4/15);  total (73/79)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCAT  >  CP009273/794290‑794581
                                                                                                                                                 |                                                                                                                                                  
tCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGcc                                                                                                                                                  >  2:2750027/1‑148 (MQ=255)
    aCGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAata                                                                                                                                              <  1:96527/148‑1 (MQ=255)
     cGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAatat                                                                                                                                             <  2:2570136/148‑1 (MQ=255)
     cGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAatat                                                                                                                                             >  1:1853941/1‑148 (MQ=255)
      gCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATAtc                                                                                                                                            >  2:1643150/1‑148 (MQ=255)
         tatTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCa                                                                                                                                         >  1:1919983/1‑148 (MQ=255)
               cACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTAGAATGCGGGTAACGTGAGCGTAACGGGTCGGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGTTTGCCATTCCGACAATATCTCACCGGAc                                                                                                                                   <  2:1058142/148‑1 (MQ=255)
                 ccGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACaa                                                                                                                                 <  2:1532674/148‑1 (MQ=255)
                    aTCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCg                                                                                                                              >  2:878148/1‑148 (MQ=255)
                    aTCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCg                                                                                                                              >  2:876827/1‑148 (MQ=255)
                      cACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGGGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTa                                                                                                                            <  1:2624634/148‑1 (MQ=255)
                      cACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTa                                                                                                                            <  2:2032799/148‑1 (MQ=255)
                      cACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTa                                                                                                                            >  2:1823133/1‑148 (MQ=255)
                          aGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCt                                                                                                                        >  2:1349497/1‑148 (MQ=255)
                           ggggCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg                                                                                                                       <  2:1873326/148‑1 (MQ=255)
                           ggggCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg                                                                                                                       <  1:546306/148‑1 (MQ=255)
                           ggggCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg                                                                                                                       >  2:2515211/1‑148 (MQ=255)
                           ggggCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtg                                                                                                                       >  2:2515743/1‑148 (MQ=255)
                           ggggCAGTTTGTCTGTGGAGGTTCTTACAATGCGGGTAACGTGATCGTAACGCGTCTGGTAGTTGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCAGTCCTCCAATATCTCACCGGACAACCGTACGCtg                                                                                                                       <  1:2136849/148‑1 (MQ=255)
                            gggCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCtgt                                                                                                                      >  1:733469/1‑148 (MQ=255)
                               cAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTggg                                                                                                                   <  1:2834989/148‑1 (MQ=255)
                                 gTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGtt                                                                                                                 >  1:2692345/1‑148 (MQ=255)
                                  tttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTc                                                                                                                >  1:1935793/1‑148 (MQ=255)
                                  tttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTc                                                                                                                >  2:326191/1‑148 (MQ=255)
                                   ttGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTcc                                                                                                               >  2:952403/1‑148 (MQ=255)
                                      tCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGc                                                                                                            <  2:3040458/148‑1 (MQ=255)
                                       cTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCa                                                                                                           >  1:2294154/1‑148 (MQ=255)
                                       cTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCa                                                                                                           <  1:702832/148‑1 (MQ=255)
                                          tGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTa                                                                                                        <  2:1777832/148‑1 (MQ=255)
                                           gTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGTTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTaa                                                                                                       <  2:3093467/148‑1 (MQ=255)
                                           gTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTaa                                                                                                       <  1:2702640/148‑1 (MQ=255)
                                            tAGGTTCTTACCATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTaaa                                                                                                      <  2:2240352/148‑1 (MQ=255)
                                             aGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAg                                                                                                     <  1:275501/148‑1 (MQ=255)
                                             aGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAg                                                                                                     >  2:319143/1‑148 (MQ=255)
                                              ggTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGc                                                                                                    >  1:61549/1‑148 (MQ=255)
                                               gTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCa                                                                                                   >  1:2903932/1‑148 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 <  2:692633/148‑1 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgt                                                                                                 >  2:3036757/1‑147 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 <  2:1477483/148‑1 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 >  2:2763027/1‑148 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 >  1:1524219/1‑148 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 <  2:552323/148‑1 (MQ=255)
                                                 tCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAgg                                                                                                 <  2:925198/148‑1 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                <  2:566256/148‑1 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                <  2:566288/148‑1 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                >  1:2905467/1‑148 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                <  1:2750027/148‑1 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                >  1:751523/1‑148 (MQ=255)
                                                  cTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGa                                                                                                >  1:980361/1‑148 (MQ=255)
                                                   ttACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGAt                                                                                               <  2:889006/148‑1 (MQ=255)
                                                    tACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATc                                                                                              >  2:2808781/1‑148 (MQ=255)
                                                    tACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATc                                                                                              >  2:2536676/1‑148 (MQ=255)
                                                     aCAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGCCAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCg                                                                                             >  2:1136464/1‑148 (MQ=255)
                                                         tGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCAtt                                                                                         >  2:2845666/1‑148 (MQ=255)
                                                         tGCGGGTAACGTGAGCGAAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATCGGGTCGAGGGGCTGGACGGTTGCCATTCCGCCTCTTTCTCACCGGACAACCGTACGCTGTGGGGTCCGGAATTAAAGCAGGATCGCAtt                                                                                         >  2:1196244/1‑148 (MQ=255)
                                                             ggTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGcc                                                                                     <  2:1526244/148‑1 (MQ=255)
                                                                aaCGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGt                                                                                  <  2:360135/148‑1 (MQ=255)
                                                                  cGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGttt                                                                                <  2:1382295/148‑1 (MQ=255)
                                                                    tGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTAc                                                                              <  2:1911868/148‑1 (MQ=255)
                                                                      aGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACgg                                                                            <  1:3158711/148‑1 (MQ=255)
                                                                       gCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGt                                                                           <  1:1757516/148‑1 (MQ=255)
                                                                       gCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGt                                                                           <  1:1757515/148‑1 (MQ=255)
                                                                          tAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAg                                                                        <  1:1298784/148‑1 (MQ=255)
                                                                             cgcgTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCga                                                                     >  1:678823/1‑148 (MQ=255)
                                                                                gTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCgatga                                                                  <  2:2585960/148‑1 (MQ=255)
                                                                                 tCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCgatgat                                                                 >  2:621196/1‑148 (MQ=255)
                                                                                     gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTc                                                             <  2:1973214/148‑1 (MQ=255)
                                                                                     gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGAACGCATTTGCCTGTTTACGGTCAGCGATGATGGTc                                                             >  1:2052976/1‑148 (MQ=255)
                                                                                     gAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGAACGCATTTGCCTGTTTACGGTCAGCGATGATGGTc                                                             >  1:1832630/1‑148 (MQ=255)
                                                                                       aGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAt                                                           <  1:598706/148‑1 (MQ=255)
                                                                                       aGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAt                                                           <  2:261218/148‑1 (MQ=255)
                                                                                        gATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAtc                                                          <  2:60947/148‑1 (MQ=255)
                                                                                        gATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAtc                                                          <  2:1735479/148‑1 (MQ=255)
                                                                                         aTGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAtct                                                         <  2:2658794/148‑1 (MQ=255)
                                                                                         aTGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCAtct                                                         <  2:3049019/148‑1 (MQ=255)
                                                                                                       ggCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGAcc                                           <  2:2982075/148‑1 (MQ=255)
                                                                                                       ggCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCAGTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGAcc                                           <  2:2982082/148‑1 (MQ=255)
                                                                                                        gCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGAccc                                          >  2:510601/1‑148 (MQ=255)
                                                                                                                 aTGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATCTCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGt                                 <  2:2080260/148‑1 (MQ=255)
                                                                                                                 aTGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGt                                 >  2:1584481/1‑148 (MQ=255)
                                                                                                                 aTGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGt                                 >  2:354250/1‑148 (MQ=255)
                                                                                                                 aTGTGGTCGAGGGGCTGGACGGTTGCCATTCAGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGt                                 <  1:2717439/148‑1 (MQ=255)
                                                                                                                   gtgGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGa                               >  1:257585/1‑148 (MQ=255)
                                                                                                                    tgGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGac                              >  2:747808/1‑148 (MQ=255)
                                                                                                                       tCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCGCCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGaccac                           >  1:168056/1‑148 (MQ=255)
                                                                                                                       tCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGaccac                           >  1:169322/1‑148 (MQ=255)
                                                                                                                       tCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGaccac                           >  1:1211374/1‑148 (MQ=255)
                                                                                                                             ggCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGa                     <  1:2536676/148‑1 (MQ=255)
                                                                                                                              gCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGaa                    >  1:941610/1‑148 (MQ=255)
                                                                                                                               cTGGACGGGTGCCAGTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAg                   <  2:2021014/148‑1 (MQ=255)
                                                                                                                                 ggACGGTTGCCATTCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAggg                 >  1:2680825/1‑148 (MQ=255)
                                                                                                                                  gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATTGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAgggg                <  2:2550263/148‑1 (MQ=255)
                                                                                                                                  gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATTGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAgggg                <  2:1328948/148‑1 (MQ=255)
                                                                                                                                  gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAgggg                >  1:548332/1‑148 (MQ=255)
                                                                                                                                  gACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAgggg                <  1:1052593/148‑1 (MQ=255)
                                                                                                                                       ttGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGggccggc           >  2:211590/1‑148 (MQ=255)
                                                                                                                                        tGCCAGTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGggccggcc          <  2:1853959/148‑1 (MQ=255)
                                                                                                                                          ccATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCcg        >  2:2319498/1‑148 (MQ=255)
                                                                                                                                          ccATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCcg        >  2:2317315/1‑148 (MQ=255)
                                                                                                                                             ttCCTCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGt     >  1:3024070/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCAT  >  CP009273/794290‑794581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑