Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 96,977 C→A 15.4% H283N (CAT→AAT)  murG → N‑acetylglucosaminyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927396,9770CA15.4% 357.2 / 31.0 138H283N (CAT→AAT) murGN‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base C (50/65);  new base A (10/13);  total (60/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAA  >  CP009273/96830‑97123
                                                                                                                                                   |                                                                                                                                                  
gggCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAc                                                                                                                                                    <  2:987726/148‑1 (MQ=255)
    aaCCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATaa                                                                                                                                                <  2:1271434/148‑1 (MQ=255)
    aaCCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATaa                                                                                                                                                <  2:1271367/148‑1 (MQ=255)
    aaCCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATaa                                                                                                                                                <  2:128856/148‑1 (MQ=255)
      ccGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTTTGGCGTTTCAAAATAAAg                                                                                                                                              >  1:2418769/1‑148 (MQ=255)
       cGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGa                                                                                                                                             <  1:2736402/148‑1 (MQ=255)
           gcaTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTTCCGTTTCAACATAAAGACCGc                                                                                                                                         >  2:2154411/1‑148 (MQ=255)
           gcaTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGc                                                                                                                                         >  2:2154674/1‑148 (MQ=255)
           gcaTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGc                                                                                                                                         <  1:2187559/148‑1 (MQ=255)
            caTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGTCTCCCGGCGTTGTTTGTGCCGTTTCAACAGAAAGACCGcc                                                                                                                                        >  2:3190686/1‑148 (MQ=255)
            caTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGcc                                                                                                                                        >  2:527862/1‑148 (MQ=255)
            caTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGAACGcc                                                                                                                                        >  2:1551199/1‑148 (MQ=255)
            caTAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGACGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGcc                                                                                                                                        <  1:1517215/148‑1 (MQ=255)
                aaGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCa                                                                                                                                    <  1:1764433/148‑1 (MQ=255)
                 aGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCac                                                                                                                                   >  2:2127887/1‑147 (MQ=255)
                 aGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCaa                                                                                                                                   <  1:2615887/148‑1 (MQ=255)
                  gTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAAt                                                                                                                                  <  2:2516795/148‑1 (MQ=255)
                  gTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAAt                                                                                                                                  <  2:2735590/148‑1 (MQ=255)
                  gTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAAt                                                                                                                                  <  2:499722/148‑1 (MQ=255)
                   tGACGGATTTTTTTGATGATTGGGCGGCGGCTTTTGGGTGGGGGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATa                                                                                                                                 <  1:1879/148‑1 (MQ=255)
                   tGACGGATTTTATTGATGTTATGGCGGCGGCGTTTGCGTGGGCGGTTGTCGTCGTTTGCCGCTCCGGTGCTTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTAAAAATAAAGACCGCCAGCAATa                                                                                                                                 <  1:1871/148‑1 (MQ=255)
                        gAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGGTAACGGTGAGTGAAATCGCCGCGGCCGGACTACCGGCGTTGTTTGTGGCGGTTAAACAAAAAGAACCCCACCaaaaaagga                                                                                                                            >  1:202476/1‑141 (MQ=255)
                         aaTTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTTGTTTTGCCCGTTCAACAAAAATACCCCCCGCACAACAGGaa                                                                                                                           >  1:1768346/1‑148 (MQ=255)
                             tATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATgcg                                                                                                                       <  2:2685684/148‑1 (MQ=255)
                               ttGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTAAAAAAAAATACCCCCCGCAAAACTTGAATgcgcg                                                                                                                     >  1:780539/1‑147 (MQ=255)
                                tgatgaTATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTa                                                                                                                    >  2:2151010/1‑148 (MQ=255)
                                tgatgaTATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCCCCAGCAAAACTGGAATgcgcga                                                                                                                    >  2:89463/1‑146 (MQ=255)
                                  atgatATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATAATGGAATGCGCTAcc                                                                                                                  >  2:2885093/1‑148 (MQ=255)
                                   tgatATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCg                                                                                                                 <  1:2820841/148‑1 (MQ=255)
                                   tgatATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCg                                                                                                                 <  1:1169721/148‑1 (MQ=255)
                                   tgatATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACAAAAAGACCGCCAGCAAAACAGGACTTGGCTACCg                                                                                                                 >  1:538774/1‑148 (MQ=255)
                                     atatGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCt                                                                                                               <  2:194208/148‑1 (MQ=255)
                                      tatGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTTACGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTTTGCCGGTTTAAAATAAAGAACCCCACCAAAAATGGAATTCGCTAACGGTg                                                                                                              >  2:2269030/1‑148 (MQ=255)
                                       atGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTgg                                                                                                             <  2:2819441/148‑1 (MQ=255)
                                       atGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTgg                                                                                                             <  2:2819429/148‑1 (MQ=255)
                                        tGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGGTTGTGGCGTTTAAAAATAAAGAACCCCACCAAAACTTGAATGCGCGAACCCTggg                                                                                                            >  1:3031745/1‑147 (MQ=255)
                                           cggcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGaaaa                                                                                                         <  1:3108215/148‑1 (MQ=255)
                                           cggcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGaaaa                                                                                                         <  1:2455675/148‑1 (MQ=255)
                                           cggcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGaaaa                                                                                                         <  1:1465227/148‑1 (MQ=255)
                                             gcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACTGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAg                                                                                                       <  2:2442099/148‑1 (MQ=255)
                                             gcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAg                                                                                                       <  2:2442070/148‑1 (MQ=255)
                                             gcggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAg                                                                                                       <  2:2441755/148‑1 (MQ=255)
                                               ggcgTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCg                                                                                                     <  1:2953687/148‑1 (MQ=255)
                                                    aTGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCCATACTGGAATGCGCGACCGCTGGAAAAAGCGGgcgc                                                                                                >  2:2091709/1‑148 (MQ=255)
                                                              ggATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAAtt                                                                                      <  2:1608146/148‑1 (MQ=255)
                                                               gttgtgGTCTTTTGCGGCTCGGGTGCTTTAACGTTGAGTTAATTCGCCGCGGCAGGACTACCGGGTTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTa                                                                                     <  1:3046027/146‑1 (MQ=255)
                                                                aTGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTAt                                                                                    <  2:1927212/148‑1 (MQ=255)
                                                                aTGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACAGAAAGACCCCCAGCAAAAAAGGAATGCGCGACCGCTGGAAAAAACGGGCGCCGCCAAAATTAt                                                                                    >  2:981338/1‑148 (MQ=255)
                                                                aTGTCGTCGTTTGCCGCTCCGGTGCGGTAACCGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTAAAAAAAAAGACCGCCACCCATACTGGCAATCGCTACCCCTGGAAAAATCGGGCGCCCCCAAAAatat                                                                                    >  2:913861/1‑148 (MQ=255)
                                                                    cgtcgtTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGag                                                                                <  1:1180902/148‑1 (MQ=255)
                                                                       cgtTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGAACGCCAGCAAAACAGGAATGCGCGACCGCGGGAAAAAGCGGGCGCAGCCAAAAATATCGagcag                                                                             >  1:1596643/1‑148 (MQ=255)
                                                                         tttGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGcc                                                                           <  1:2157582/148‑1 (MQ=255)
                                                                             ccGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAg                                                                       >  2:1734175/1‑148 (MQ=255)
                                                                              cGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGc                                                                      <  1:1105427/148‑1 (MQ=255)
                                                                              cGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGc                                                                      <  1:1105530/148‑1 (MQ=255)
                                                                              cGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGc                                                                      <  1:989771/148‑1 (MQ=255)
                                                                              cGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGc                                                                      <  1:989676/148‑1 (MQ=255)
                                                                               gCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCt                                                                     <  2:3204729/148‑1 (MQ=255)
                                                                               gCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGAATACGGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGAAATACTGTAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCt                                                                     <  1:679571/148‑1 (MQ=255)
                                                                                cTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCtt                                                                    <  2:1815331/148‑1 (MQ=255)
                                                                                 tCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTa                                                                   <  1:2957924/148‑1 (MQ=255)
                                                                                    ggTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCg                                                                <  2:395852/148‑1 (MQ=255)
                                                                                       gCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGGTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTgg                                                             <  2:62767/148‑1 (MQ=255)
                                                                                        cGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGa                                                            <  2:19504/148‑1 (MQ=255)
                                                                                        cGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGa                                                            <  2:19506/148‑1 (MQ=255)
                                                                                          ttAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATg                                                          <  2:2865606/148‑1 (MQ=255)
                                                                                             aCGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTTTGCCGTTTCAACATAAATACCGCCAGCAAAACTGGAATGCGCTAACGCTGGGAAAAGCGGGGGCAGCCAAAAATATCGAGCACCCACAGCGTAGCGTGGctgctg                                                       >  1:449611/1‑148 (MQ=255)
                                                                                             aCGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGAACCCCACCAATACTGGAAATCGCGACCCCAGGAAAAAGCGGGCGCCGCCAAAAATATCGAGCAGCCACCGCGTAGCGTGGATGCTg                                                       >  1:449642/1‑148 (MQ=255)
                                                                                                gtgagtgaAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTTGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTct                                                    >  2:2389901/1‑147 (MQ=255)
                                                                                                gtgagtgaAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCg                                                    >  2:2389735/1‑148 (MQ=255)
                                                                                                             ccgcGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGc                                       <  2:2965967/148‑1 (MQ=255)
                                                                                                              cgcgGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGcc                                      <  1:1041272/148‑1 (MQ=255)
                                                                                                              cgcgGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGcc                                      <  2:3273241/148‑1 (MQ=255)
                                                                                                                cgGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGTAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCgg                                    <  2:1680676/148‑1 (MQ=255)
                                                                                                                cgGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGTAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCgg                                    <  2:1170073/148‑1 (MQ=255)
                                                                                                                     ggtcttacGGCGTTGTTTGTGCCGTTTCAACATACAGACCGCCATCAATACTGGTATGCGCTACCGCTGTAAAAAGCGGGCGCAGCCAAAATTATCTAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCTACAGCCTGGCCGggtggt                               <  2:2771499/141‑1 (MQ=255)
                                                                                                                     ggACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGggtggt                               >  2:2171682/1‑148 (MQ=255)
                                                                                                                     ggACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGggtggt                               >  2:509225/1‑148 (MQ=255)
                                                                                                                     ggACTACCGGCGTTGTTTGTGCCGTTTAAACATAAAGAACGCCAGCAATACTGGAATTCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAAATATAGAGCAGCCACAGCGTAGCGTGGATGCTGTCGCCAACACCCTGGCCGggtggt                               >  1:810363/1‑148 (MQ=255)
                                                                                                                      gACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCCCCAGCAATAATGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCCAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTc                              >  2:3134175/1‑148 (MQ=255)
                                                                                                                      gACTACCGGCGTTGTTTGTGCCGTTTAAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTc                              >  2:3134178/1‑148 (MQ=255)
                                                                                                                         tACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTcgcg                           <  1:2340028/148‑1 (MQ=255)
                                                                                                                             ggCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaa                       >  2:227324/1‑148 (MQ=255)
                                                                                                                             ggCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaa                       >  2:671659/1‑148 (MQ=255)
                                                                                                                              gCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaag                      <  2:1071712/148‑2 (MQ=255)
                                                                                                                              gCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaaa                      <  2:2724750/148‑1 (MQ=255)
                                                                                                                              gCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaaa                      <  1:691191/148‑1 (MQ=255)
                                                                                                                              gCGTTGTTTGTGCCGTTTCAAAATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGaaa                      >  2:1225630/1‑148 (MQ=255)
                                                                                                                                gttgttTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAAcc                    >  1:360371/1‑148 (MQ=255)
                                                                                                                                 ttgtttgtGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCt                   >  2:1653153/1‑148 (MQ=255)
                                                                                                                                 ttgtttgtGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCt                   >  1:140946/1‑148 (MQ=255)
                                                                                                                                  tgtttgtGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCtt                  >  2:1612334/1‑148 (MQ=255)
                                                                                                                                    tttgtGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCttat                >  2:796907/1‑148 (MQ=255)
                                                                                                                                      tgtgCCGTTTCAACATAAAGACCGACAGAAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCttatta              <  1:1126583/148‑1 (MQ=255)
                                                                                                                                        tgCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAAc            >  2:2937593/1‑148 (MQ=255)
                                                                                                                                          ccGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCa          <  2:1913068/148‑1 (MQ=255)
                                                                                                                                           cGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCAt         >  2:580337/1‑148 (MQ=255)
                                                                                                                                              ttCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGc      >  1:92085/1‑148 (MQ=255)
                                                                                                                                              ttCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGc      >  1:1743448/1‑148 (MQ=255)
                                                                                                                                                  aCATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGaa  <  1:383954/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCAACCGCAGCATAAAGTGACGGAATTTATTGATGATATGGCGGCGGCGTATGCGTGGGCGGATGTCGTCGTTTGCCGCTCCGGTGCGTTAACGGTGAGTGAAATCGCCGCGGCAGGACTACCGGCGTTGTTTGTGCCGTTTCAACATAAAGACCGCCAGCAATACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAA  >  CP009273/96830‑97123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑