Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 850,161 G→T 87.1% P353P (CCG→CCT)  ybiR → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273850,1610GT87.1% 299.8 / 42.2 141P353P (CCG→CCT) ybiRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base T (58/64);  total (66/75)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 141 total aligned reads displayed.

CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGG  >  CP009273/850017‑850305
                                                                                                                                                |                                                                                                                                                
   tgtgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtg                                                                                                                                            <  2:2042761/148‑1 (MQ=255)
       ccgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgttgt                                                                                                                                        <  2:1845191/148‑1 (MQ=255)
             tCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcgg                                                                                                                                  >  2:497089/1‑148 (MQ=255)
             tCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcgg                                                                                                                                  >  2:2474154/1‑148 (MQ=255)
              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  1:2079905/1‑148 (MQ=255)
              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  2:2535630/1‑148 (MQ=255)
              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  2:2455935/1‑148 (MQ=255)
               tttATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGgcggcg                                                                                                                                <  2:1242467/148‑1 (MQ=255)
               tttATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGAATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGgcggcg                                                                                                                                >  2:2035897/1‑148 (MQ=255)
                ttattaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGt                                                                                                                               <  1:1092131/148‑1 (MQ=255)
                  attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtg                                                                                                                             <  1:2091126/148‑1 (MQ=255)
                  attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtg                                                                                                                             <  1:1540324/148‑1 (MQ=255)
                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     <  2:2272739/148‑1 (MQ=255)
                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  1:1864502/1‑148 (MQ=255)
                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  2:467444/1‑148 (MQ=255)
                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGcta                                                                                                                     >  1:46316/1‑148 (MQ=255)
                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAAAACCTATGCTGGTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  1:46330/1‑148 (MQ=255)
                              ggCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGtt                                                                                                                 <  2:483898/148‑1 (MQ=255)
                               gCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGttt                                                                                                                <  1:2353861/148‑1 (MQ=255)
                                cGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGtttt                                                                                                               <  2:3046549/148‑1 (MQ=255)
                                 ggTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTg                                                                                                              <  2:3250100/148‑1 (MQ=255)
                                 ggTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTg                                                                                                              <  2:1707439/148‑1 (MQ=255)
                                  gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGGTTTgt                                                                                                             >  1:1007800/1‑148 (MQ=255)
                                   tAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtt                                                                                                            >  1:3247008/1‑148 (MQ=255)
                                      aCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                         >  2:2965312/1‑148 (MQ=255)
                                      aCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                         >  2:541433/1‑148 (MQ=255)
                                          aGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                     <  1:243139/148‑1 (MQ=255)
                                           ggTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAc                                                                                                    >  1:608104/1‑148 (MQ=255)
                                             tGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTc                                                                                                  <  2:922074/148‑1 (MQ=255)
                                                cTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCg                                                                                               >  2:3209975/1‑148 (MQ=255)
                                                    gggTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCa                                                                                           <  1:562964/148‑1 (MQ=255)
                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  1:493352/1‑148 (MQ=255)
                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  1:3275377/1‑148 (MQ=255)
                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        <  1:1293044/148‑1 (MQ=255)
                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  2:1375486/1‑148 (MQ=255)
                                                        tattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTa                                                                                       >  2:3209863/1‑148 (MQ=255)
                                                        tattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGGTATACTCCCGGCCAACTa                                                                                       >  2:2623891/1‑148 (MQ=255)
                                                          ttaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAgg                                                                                     <  2:639663/148‑1 (MQ=255)
                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCt                                                                                 >  1:3190008/1‑148 (MQ=255)
                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTAAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCt                                                                                 >  1:802210/1‑148 (MQ=255)
                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  1:2188221/148‑1 (MQ=255)
                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  2:2457673/148‑1 (MQ=255)
                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  1:24985/148‑1 (MQ=255)
                                                                  aTCGCTGGCAAATTTGATTGCGATACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCa                                                                             >  2:1425221/1‑148 (MQ=255)
                                                                   tCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaa                                                                            <  2:154654/148‑1 (MQ=255)
                                                                   tCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaa                                                                            >  1:101533/1‑148 (MQ=255)
                                                                    cGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaaa                                                                           <  2:610705/148‑1 (MQ=255)
                                                                       tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaa                                                                        <  2:2814210/148‑1 (MQ=255)
                                                                       tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaa                                                                        <  2:2869423/148‑1 (MQ=255)
                                                                         gCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaa                                                                      >  2:1325197/1‑148 (MQ=255)
                                                                          cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaa                                                                     >  2:965732/1‑148 (MQ=255)
                                                                           aaaTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                    <  1:2535630/148‑1 (MQ=255)
                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  >  1:477486/1‑148 (MQ=255)
                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  <  2:145611/148‑1 (MQ=255)
                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  <  2:145679/148‑1 (MQ=255)
                                                                              tttGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGc                                                                 >  2:1948978/1‑148 (MQ=255)
                                                                                tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                               <  1:1223538/148‑1 (MQ=255)
                                                                                tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                               <  1:1223570/148‑1 (MQ=255)
                                                                                 gATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGa                                                              <  2:3247008/148‑1 (MQ=255)
                                                                                   ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAtt                                                            <  1:1437161/148‑1 (MQ=255)
                                                                                    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                           <  2:2217505/148‑1 (MQ=255)
                                                                                    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                           <  1:2615102/148‑1 (MQ=255)
                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  1:1646576/148‑1 (MQ=255)
                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  1:802723/148‑1 (MQ=255)
                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  2:1524478/148‑1 (MQ=255)
                                                                                        cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                       <  2:412719/148‑1 (MQ=255)
                                                                                                    aaCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                           <  1:1243684/148‑1 (MQ=255)
                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                          <  2:3098190/148‑1 (MQ=255)
                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTCCATCTCGATTCAATACCTATGCGGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCCACTAGGGTCTGGCAAATAAAAAAGGCGGATTATTATTCCGCCGTTTTCt                                          >  1:2263378/1‑148 (MQ=255)
                                                                                                     aCGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTAAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTTTTATTTATACTCCCGGCCAACTAGGGTCTGGCCAATAAAAAAGGCGGATTATTATTCCGCCTTTTTCt                                          >  1:2263390/1‑148 (MQ=255)
                                                                                                      cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCtt                                         >  2:1785981/1‑148 (MQ=255)
                                                                                                        aTCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                       <  1:2684044/148‑1 (MQ=255)
                                                                                                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAg                                     <  2:1045135/148‑1 (MQ=255)
                                                                                                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAg                                     <  2:2735379/148‑1 (MQ=255)
                                                                                                              gcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtttt                                 >  1:1809180/1‑148 (MQ=255)
                                                                                                               cATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                <  1:1447837/148‑1 (MQ=255)
                                                                                                                aTCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGa                               <  1:3049394/148‑1 (MQ=255)
                                                                                                                 tCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAAAAAAAAATGCGGATTATTAGTCCGCCTTTTGCTTTAAGTTTTTaa                              >  1:139353/1‑148 (MQ=255)
                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTGGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                             <  1:2048145/148‑1 (MQ=255)
                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                             <  1:3240198/148‑1 (MQ=255)
                                                                                                                   tggtggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                            <  1:2733761/148‑1 (MQ=255)
                                                                                                                   tggtggCGTTTCCATCTCTATTCAAGACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                            <  2:307874/148‑1 (MQ=255)
                                                                                                                    ggtggCGTTTCCATCTCTATTAAATACCTATGGTGTTTTGGGCGGCGTTGGTGGGTGATGTTTTGTTAGTTATACTCCCGGCCAAATAGGGTCTGGCAAATAAAAAAGGAGTATTATGAGTACGCCTTTTGCTTTAAGTTTTGAAGAt                           >  2:2029098/1‑148 (MQ=255)
                                                                                                                       ggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTaa                        <  1:2473645/148‑1 (MQ=255)
                                                                                                                        gCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:840127/148‑1 (MQ=255)
                                                                                                                        gCGTGTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:840245/148‑1 (MQ=255)
                                                                                                                        gCGGTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:2212805/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                      <  2:818232/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                      <  2:2286326/148‑1 (MQ=255)
                                                                                                                           tttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                    >  2:2683469/1‑148 (MQ=255)
                                                                                                                           tttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                    >  1:3278845/1‑148 (MQ=255)
                                                                                                                              ccATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGAc                 >  1:2161373/1‑148 (MQ=255)
                                                                                                                                aTCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa               >  2:2501357/1‑148 (MQ=255)
                                                                                                                                 tctcTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGaa              <  2:2597128/148‑1 (MQ=255)
                                                                                                                                   tctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc            >  1:2884347/1‑148 (MQ=255)
                                                                                                                                   tctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc            >  1:1635865/1‑148 (MQ=255)
                                                                                                                                      aTTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc         <  1:958766/148‑1 (MQ=255)
                                                                                                                                        tCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt       <  1:467444/148‑1 (MQ=255)
                                                                                                                                          aaTACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc     <  2:2260356/148‑1 (MQ=255)
                                                                                                                                            tACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg   >  2:1764042/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGG  >  CP009273/850017‑850305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑