BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	854,482	G→A	5.8%	A12A (GCC→GCT)	ybiU ←	DUF1479 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	854,482	0	G	A	5.8%	691.6 / 19.1	190	A12A (GCC→GCT)	ybiU	DUF1479 family protein
Reads supporting (aligned to +/- strand): ref base G (64/115); new base A (5/6); total (69/121)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 197 total aligned reads displayed.

ACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGT  >  CP009273/854339‑854626

aCGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCggcag                                                                                                                                              >  1:2336837/1‑148 (MQ=255)

aCGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCggcag                                                                                                                                              >  1:1159533/1‑148 (MQ=255)

aCGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCggcag                                                                                                                                              >  1:1176018/1‑148 (MQ=255)

   gCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCggcaggca                                                                                                                                           <  2:1421281/148‑1 (MQ=255)

    cATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCaa                                                                                                                                          <  1:644921/148‑1 (MQ=255)

    cATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCaa                                                                                                                                          <  1:1890585/148‑1 (MQ=255)

      tCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAAtg                                                                                                                                        >  1:2949313/1‑148 (MQ=255)

      tCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAAtg                                                                                                                                        >  2:733009/1‑148 (MQ=255)

      tCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAAtg                                                                                                                                        <  1:1225387/148‑1 (MQ=255)

           cTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCg                                                                                                                                   >  1:1195400/1‑148 (MQ=255)

           cTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCg                                                                                                                                   >  1:942265/1‑148 (MQ=255)

                  ttGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTaa                                                                                                                            >  1:1509106/1‑148 (MQ=255)

                       tGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTa                                                                                                                       >  1:2828514/1‑148 (MQ=255)

                        gCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAg                                                                                                                      >  1:793393/1‑148 (MQ=255)

                         cGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGa                                                                                                                     <  2:478950/148‑1 (MQ=255)

                             gATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGcc                                                                                                                 <  1:383461/148‑1 (MQ=255)

                              aTTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCa                                                                                                                >  1:1771886/1‑148 (MQ=255)

                               tttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCAt                                                                                                               >  1:593968/1‑148 (MQ=255)

                                ttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATa                                                                                                              <  1:2009288/148‑1 (MQ=255)

                                ttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATa                                                                                                              <  1:342585/148‑1 (MQ=255)

                                ttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATa                                                                                                              <  2:1336145/148‑1 (MQ=255)

                                 tCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATaa                                                                                                             <  1:1390892/148‑1 (MQ=255)

                                 tCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATaa                                                                                                             <  1:795742/148‑1 (MQ=255)

                                  cAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAac                                                                                                            <  1:2122451/148‑1 (MQ=255)

                                     ccACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAaccac                                                                                                         >  1:303876/1‑148 (MQ=255)

                                      cACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAaccacc                                                                                                        <  2:3087554/148‑1 (MQ=255)

                                       aCTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACct                                                                                                       <  2:668703/148‑1 (MQ=255)

                                         tCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACctct                                                                                                     >  2:1077513/1‑148 (MQ=255)

                                         tCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACctct                                                                                                     <  2:1168791/148‑1 (MQ=255)

                                           gcgTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCa                                                                                                   <  1:1154623/148‑1 (MQ=255)

                                            cgTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCAt                                                                                                  >  1:633972/1‑148 (MQ=255)

                                            cgTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCAt                                                                                                  <  1:1298223/148‑1 (MQ=255)

                                               ggCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCAGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTaa                                                                                               <  1:1797058/148‑1 (MQ=255)

                                                 cAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACt                                                                                             <  1:733009/148‑1 (MQ=255)

                                                    tGTCATCGCTTAGCTGATTAAAGATCTGCTGTACCTCGCCAAGCTGCGCCCGCCGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAAGGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTGTc                                                                                          >  2:1430179/1‑148 (MQ=255)

                                                    tGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTc                                                                                          <  1:1387170/148‑1 (MQ=255)

                                                     gTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCa                                                                                         <  1:2506596/148‑1 (MQ=255)

                                                         tCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCg                                                                                     >  2:472211/1‑148 (MQ=255)

                                                          cGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGa                                                                                    <  1:1040676/148‑1 (MQ=255)

                                                             ttAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAAc                                                                                 <  2:1245119/148‑1 (MQ=255)

                                                              tAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAAcc                                                                                >  1:2215543/1‑148 (MQ=255)

                                                                gCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCAt                                                                              <  1:3190943/148‑1 (MQ=255)

                                                                gCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCAGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCAt                                                                              >  2:1607282/1‑148 (MQ=255)

                                                                 cTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCAtt                                                                             <  2:2706691/148‑1 (MQ=255)

                                                                 cTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCAtt                                                                             <  2:2147411/148‑1 (MQ=255)

                                                                  tGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTc                                                                            <  1:2553152/148‑1 (MQ=255)

                                                                    aTTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTg                                                                          <  1:2467671/148‑1 (MQ=255)

                                                                      tAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCAGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGAt                                                                        >  2:1696540/1‑148 (MQ=255)

                                                                         aGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTg                                                                     >  1:1551256/1‑148 (MQ=255)

                                                                         aGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTg                                                                     >  1:2597429/1‑148 (MQ=255)

                                                                             ctgctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCAtg                                                                 <  1:2659738/148‑1 (MQ=255)

                                                                               gctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCAtgtg                                                               <  1:1323830/148‑1 (MQ=255)

                                                                                ctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGa                                                              <  2:1218763/148‑1 (MQ=255)

                                                                                ctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGa                                                              <  2:2120870/148‑1 (MQ=255)

                                                                                  ggACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGAATGTGACa                                                            >  1:1871618/1‑148 (MQ=255)

                                                                                   gACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACaa                                                           <  2:1079741/148‑1 (MQ=255)

                                                                                   gACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACaa                                                           <  1:2858155/148‑1 (MQ=255)

                                                                                     cGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAata                                                         <  2:3148089/148‑1 (MQ=255)

                                                                                     cGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAata                                                         <  2:2292004/148‑1 (MQ=255)

                                                                                              gCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTtata                                                >  1:120770/1‑148 (MQ=255)

                                                                                              gCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTtata                                                >  2:1153699/1‑148 (MQ=255)

                                                                                               cTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTataa                                               <  2:1802995/148‑1 (MQ=255)

                                                                                                tGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTataaa                                              <  2:1599895/148‑1 (MQ=255)

                                                                                                 gcgcCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTataaat                                             >  1:2040066/1‑148 (MQ=255)

                                                                                                  cgcCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTataaata                                            >  1:2820489/1‑148 (MQ=255)

                                                                                                    cccGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAg                                          <  2:582559/148‑1 (MQ=255)

                                                                                                       gCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTa                                       <  2:821935/148‑1 (MQ=255)

                                                                                                          gcgcGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATg                                    <  1:249662/148‑1 (MQ=255)

                                                                                                           cgcgTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGc                                   >  2:2026528/1‑148 (MQ=255)

                                                                                                            gcgTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCa                                  <  1:574310/148‑1 (MQ=255)

                                                                                                             cgTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCaa                                 <  1:419553/148‑1 (MQ=255)

                                                                                                                gCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGAATGTGACAATATAAGTTATAAATAAGTTAATGCAAGtt                              <  1:2977100/148‑1 (MQ=255)

                                                                                                                 cTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGttt                             >  1:326194/1‑148 (MQ=255)

                                                                                                                  ttCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTa                            >  1:841342/1‑148 (MQ=255)

                                                                                                                  ttCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTa                            <  2:2613418/148‑1 (MQ=255)

                                                                                                                  ttCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTa                            <  1:27785/148‑1 (MQ=255)

                                                                                                                  ttCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTa                            <  1:27757/148‑1 (MQ=255)

                                                                                                                   tCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTaa                           <  2:343314/148‑1 (MQ=255)

                                                                                                                   tCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTaa                           <  1:2416907/148‑1 (MQ=255)

                                                                                                                     aTCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATg                         <  2:1176366/148‑1 (MQ=255)

                                                                                                                     aTCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATg                         <  2:1159533/148‑1 (MQ=255)

                                                                                                                     aTCTGACGGATAGCTGCTTTGTGATCAGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATg                         <  1:1607282/148‑1 (MQ=255)

                                                                                                                      tCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGa                        >  1:2299702/1‑148 (MQ=255)

                                                                                                                      tCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGa                        <  1:1578876/148‑1 (MQ=255)

                                                                                                                       cTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGAt                       <  2:187377/148‑1 (MQ=255)

                                                                                                                        tGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGAtt                      <  2:2542972/148‑1 (MQ=255)

                                                                                                                           cGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtga                   <  1:1016425/148‑1 (MQ=255)

                                                                                                                             gATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatg                 <  2:942265/148‑1 (MQ=255)

                                                                                                                             gATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatg                 <  2:1195400/148‑1 (MQ=255)

                                                                                                                                aGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatgtga              <  2:2475473/148‑1 (MQ=255)

                                                                                                                                aGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatgtga              <  1:3115474/148‑1 (MQ=255)

                                                                                                                                 gctgctTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatgtgat             >  2:781337/1‑148 (MQ=255)

                                                                                                                                  ctgctTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTtgatgtgatg            <  1:2901477/148‑1 (MQ=255)

                                                                                                                                   tgctTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTgatgtgatgt           <  1:3054432/148‑1 (MQ=255)

                                                                                                                                      tttGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACa        <  2:2948048/148‑1 (MQ=255)

                                                                                                                                      tttGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACa        <  2:1194479/148‑1 (MQ=255)

                                                                                                                                       ttGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAg       >  2:1522099/1‑148 (MQ=255)

                                                                                                                                        tgtgATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAgg      <  1:484083/148‑1 (MQ=255)

                                                                                                                                          tgATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGAt    <  2:2040732/148‑1 (MQ=255)

                                                                                                                                          tgATCAGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGAt    >  2:1554023/1‑148 (MQ=255)

ACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGCAAGTTTAATGATTTGATGTGATGTACAGGATGT  >  CP009273/854339‑854626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`