Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,672 G→T 87.5% L42I (CTC→ATC)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,6720GT87.5% 490.2 / 74.3 214L42I (CTC→ATC) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (10/17);  new base T (99/88);  total (109/105)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 220 total aligned reads displayed.

CGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882528‑882818
                                                                                                                                                |                                                                                                                                                  
 gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGataata                                                                                                                                                <  2:2272536/148‑1 (MQ=255)
   tCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                              >  1:3146018/1‑148 (MQ=255)
    cAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcg                                                                                                                                             <  2:1911987/148‑1 (MQ=255)
    cAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcg                                                                                                                                             >  1:817076/1‑148 (MQ=255)
      aTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcgcg                                                                                                                                           <  1:1918014/148‑1 (MQ=255)
        gcgcAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGa                                                                                                                                         >  2:2085439/1‑148 (MQ=255)
           cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAg                                                                                                                                      >  1:588221/1‑148 (MQ=255)
           cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAg                                                                                                                                      >  2:1398080/1‑148 (MQ=255)
             ggACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCa                                                                                                                                    <  2:1151264/148‑1 (MQ=255)
             ggACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCa                                                                                                                                    >  2:1183856/1‑148 (MQ=255)
               aCATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAgg                                                                                                                                  >  2:3312328/1‑148 (MQ=255)
                   cgcgACCAGGATGACATTACGTGTCCCAAAGCGTGTCACTAACCCCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                              <  2:811499/145‑1 (MQ=255)
                   cGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                              >  2:1085237/1‑148 (MQ=255)
                   cGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                              >  2:1543060/1‑148 (MQ=255)
                   cGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                              <  2:811533/148‑1 (MQ=255)
                           ggATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGc                                                                                                                      >  2:457883/1‑148 (MQ=255)
                            gATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATCATATCGCGGATCGCAGGCGTACGGGTTGcc                                                                                                                     >  2:1723385/1‑148 (MQ=255)
                             aTGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGccc                                                                                                                    >  2:593936/1‑148 (MQ=255)
                             aTGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGccc                                                                                                                    >  1:1437044/1‑148 (MQ=255)
                                aCATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAgg                                                                                                                 <  1:1472401/148‑1 (MQ=255)
                                aCATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAgg                                                                                                                 <  1:1460375/148‑1 (MQ=255)
                                aCATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAgg                                                                                                                 <  1:1046191/148‑1 (MQ=255)
                                 cATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGa                                                                                                                <  1:2081371/148‑1 (MQ=255)
                                 cATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGa                                                                                                                <  1:1700724/148‑1 (MQ=255)
                                 cATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGa                                                                                                                <  1:1144358/148‑1 (MQ=255)
                                   ttACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACg                                                                                                              >  2:2713350/1‑148 (MQ=255)
                                    tACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                             >  1:75878/1‑148 (MQ=255)
                                     aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGcc                                                                                                            >  1:2681554/1‑148 (MQ=255)
                                     aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGcc                                                                                                            >  1:3039855/1‑148 (MQ=255)
                                          tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                       <  2:2343648/148‑1 (MQ=255)
                                          tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                       >  1:1656088/1‑148 (MQ=255)
                                           cccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAAc                                                                                                      <  2:1781123/148‑1 (MQ=255)
                                           cccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAAc                                                                                                      <  2:1781076/148‑1 (MQ=255)
                                           accAATGCTTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACTGACCAAAGTGTACACCGCCCATTTCAGCGATCGAGACAGAGATTATATCGCGGATAGCAGGCGTACGGGTTGCCGAGGACGCCATTAAc                                                                                                      <  1:2609295/147‑1 (MQ=255)
                                             cAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAg                                                                                                    >  2:1606445/1‑148 (MQ=255)
                                              aaaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAgg                                                                                                   <  1:425652/148‑1 (MQ=255)
                                                 gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCt                                                                                                >  2:467133/1‑148 (MQ=255)
                                                 gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCt                                                                                                >  1:915251/1‑148 (MQ=255)
                                                  cGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGCCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTg                                                                                               >  2:2904527/1‑148 (MQ=255)
                                                  cGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTg                                                                                               >  1:2733677/1‑148 (MQ=255)
                                                   gTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTgg                                                                                              <  1:860812/148‑1 (MQ=255)
                                                    tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                             >  1:1014341/1‑148 (MQ=255)
                                                          tAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTGGCCCAGGACGCCCTTAACAGGCCTGGCAAAaag                                                                                       >  2:3055090/1‑148 (MQ=255)
                                                           aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                      >  2:1202719/1‑148 (MQ=255)
                                                           aaCCACGCCGAGCAGAGAATACCGCTCATCGAACAGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                      <  1:2285054/148‑1 (MQ=255)
                                                            aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCACAcagaa                                                                                     >  2:2254522/1‑148 (MQ=255)
                                                             ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAaagaag                                                                                    >  1:1497249/1‑148 (MQ=255)
                                                             ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                    >  2:1589350/1‑148 (MQ=255)
                                                             ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                    >  2:3223317/1‑148 (MQ=255)
                                                              cACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAagaaga                                                                                   <  2:293683/148‑1 (MQ=255)
                                                                cGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTACCAGGCCTGGCAAAAAGAAGAAc                                                                                 >  1:2338092/1‑148 (MQ=255)
                                                                 gCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACa                                                                                <  1:691048/148‑1 (MQ=255)
                                                                  ccGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACAt                                                                               <  1:2471637/148‑1 (MQ=255)
                                                                  ccGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACAt                                                                               <  1:2473683/148‑1 (MQ=255)
                                                                     aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaaa                                                                            <  2:3123961/148‑1 (MQ=255)
                                                                      gCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                           <  2:2790191/148‑1 (MQ=255)
                                                                          agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                       >  1:1814630/1‑148 (MQ=255)
                                                                          agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTCACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                       >  1:2575330/1‑148 (MQ=255)
                                                                          agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                       >  2:102841/1‑148 (MQ=255)
                                                                          agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                       >  1:1814709/1‑148 (MQ=255)
                                                                            aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCTAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGcc                                                                     >  1:1768671/1‑148 (MQ=255)
                                                                                  gCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                               <  2:18234/148‑1 (MQ=255)
                                                                                   ctaaTCGAACCGATCGACAGACAAAAGAGACCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTAGGGGTTGCCCAGGACGCAATTAACAGGCCTGGCAAAAAGAAGAACATAAACCGCGCCCAGGTTc                                                              <  1:94839/145‑1 (MQ=255)
                                                                                   cTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCCGGTTc                                                              >  2:710590/1‑148 (MQ=255)
                                                                                    tCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCg                                                             <  1:2821819/148‑1 (MQ=255)
                                                                                       tCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCATGGCCAAATGAAGAACATAAAAACCGCCCAGGTTCGGCg                                                          >  2:2093913/1‑148 (MQ=255)
                                                                                        ctaaCCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTTCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGt                                                         <  2:2731932/146‑1 (MQ=255)
                                                                                        cGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGt                                                         <  2:915251/148‑1 (MQ=255)
                                                                                            ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCa                                                     <  1:1085237/148‑1 (MQ=255)
                                                                                             cGATCGACAGACCAAAGACAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCaa                                                    <  1:249040/148‑1 (MQ=255)
                                                                                               aTCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATg                                                  <  1:1734899/148‑1 (MQ=255)
                                                                                                 cGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCa                                                >  1:3102485/1‑148 (MQ=255)
                                                                                                 cGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCa                                                <  1:2743597/148‑1 (MQ=255)
                                                                                                  gACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAt                                               <  2:817076/148‑1 (MQ=255)
                                                                                                  gACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAACAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAt                                               <  1:1247583/148‑1 (MQ=255)
                                                                                                   aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAtt                                              <  1:2988303/148‑1 (MQ=255)
                                                                                                   aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAtt                                              >  1:1561841/1‑148 (MQ=255)
                                                                                                         cAAAGAGAACACCGCCCATTTCAGCGATCGAGACCGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTACCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTga                                        >  2:848211/1‑148 (MQ=255)
                                                                                                            agagAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaaga                                     <  1:1003393/148‑1 (MQ=255)
                                                                                                              agaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                   >  2:459325/1‑148 (MQ=255)
                                                                                                              agaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                   >  1:628681/1‑148 (MQ=255)
                                                                                                              agaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTCTCAATGCATTACGTGAAGAAt                                   >  2:459394/1‑148 (MQ=255)
                                                                                                                    ccGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                             <  1:2195423/148‑1 (MQ=255)
                                                                                                                    ccGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                             <  2:1471621/148‑1 (MQ=255)
                                                                                                                     cGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTCACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGt                            >  2:2785128/1‑148 (MQ=255)
                                                                                                                     cGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTGTCAATGCATTACGTGAAGAATTTACGGt                            >  2:2652731/1‑148 (MQ=255)
                                                                                                                        ccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                         <  1:822621/148‑1 (MQ=255)
                                                                                                                           tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTCACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                      >  2:2442073/1‑148 (MQ=255)
                                                                                                                           tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTCACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                      >  1:3232236/1‑148 (MQ=255)
                                                                                                                           tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                      >  2:783511/1‑148 (MQ=255)
                                                                                                                                gCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                 <  2:3226235/148‑1 (MQ=255)
                                                                                                                                gCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                 <  2:470417/148‑1 (MQ=255)
                                                                                                                                   aTCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCa              >  2:2620447/1‑148 (MQ=255)
                                                                                                                                     cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTACCAGGCCTGACAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaaa            <  1:3054296/148‑1 (MQ=255)
                                                                                                                                     cGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaaa            <  1:2974559/148‑1 (MQ=255)
                                                                                                                                     cGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaaa            >  2:911221/1‑148 (MQ=255)
                                                                                                                                       agaCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt          >  1:2110850/1‑148 (MQ=255)
                                                                                                                                           agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATacga      >  2:2843788/1‑148 (MQ=255)
                                                                                                                                           agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaaga      <  2:3039855/148‑1 (MQ=255)
                                                                                                                                            gagataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaa     <  1:202307/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAG  >  CP009273/882528‑882818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑