Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,102,093 G→T 7.8% D95Y (GAC→TAC)  clsC → stationary phase cardiolipin synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,102,0930GT 7.8% 578.4 / 19.1 167D95Y (GAC→TAC) clsCstationary phase cardiolipin synthase 3
Reads supporting (aligned to +/- strand):  ref base G (75/79);  new base T (8/5);  total (83/84)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 184 total aligned reads displayed.

GATGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGACCCTGGTG  >  CP009273/1101946‑1102239
                                                                                                                                                   |                                                                                                                                                  
gATGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGcttgcttg                                                                                                                                                    <  1:2582111/148‑1 (MQ=255)
gATGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGcttgcttg                                                                                                                                                    <  1:3281510/148‑1 (MQ=255)
 aTGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGa                                                                                                                                                   <  2:304149/148‑1 (MQ=255)
  tGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCATGCTTGAc                                                                                                                                                  >  1:1917054/1‑148 (MQ=255)
   gTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACa                                                                                                                                                 >  2:1751524/1‑148 (MQ=255)
   gTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACa                                                                                                                                                 >  2:2458249/1‑148 (MQ=255)
    ttCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAg                                                                                                                                                >  1:2699650/1‑148 (MQ=255)
          aTTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATcc                                                                                                                                          <  2:2015995/148‑1 (MQ=255)
          aTTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAAAACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATcc                                                                                                                                          >  1:933781/1‑148 (MQ=255)
           ttACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCa                                                                                                                                         <  2:3196781/148‑1 (MQ=255)
              cATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGc                                                                                                                                      <  2:3265457/148‑1 (MQ=255)
              cATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTACGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGTCGACAACAATACGCCCGGCCTTGACGACATTTTAAGCTTGCGTGACAGTCATacaccc                                                                                                                                      >  1:623244/1‑146 (MQ=255)
                tCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCAt                                                                                                                                    >  2:2486198/1‑148 (MQ=255)
                   ggCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGa                                                                                                                                 <  1:2389911/148‑1 (MQ=255)
                    gCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGaa                                                                                                                                <  1:1009143/148‑1 (MQ=255)
                    gCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGaa                                                                                                                                <  1:556519/148‑1 (MQ=255)
                        gACGATATGTCGGGTCGGTTACTGTTTTCCGCCCGGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCAACCACGCATTGAAGTcc                                                                                                                            >  1:1751984/1‑148 (MQ=255)
                           gATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGc                                                                                                                         <  2:1478145/148‑1 (MQ=255)
                           gATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGc                                                                                                                         <  2:185251/148‑1 (MQ=255)
                                  cgggtcggTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTTCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTaa                                                                                                                  >  1:2160114/1‑148 (MQ=255)
                                     gtcggTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATcc                                                                                                               <  2:1491844/148‑1 (MQ=255)
                                     gtcggTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATcc                                                                                                               <  2:1369515/148‑1 (MQ=255)
                                         gTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTtc                                                                                                           <  1:2765835/148‑1 (MQ=255)
                                         gTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTtc                                                                                                           <  1:2788670/148‑1 (MQ=255)
                                          ttACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTtct                                                                                                          <  2:2814135/148‑1 (MQ=255)
                                          ttACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTtct                                                                                                          <  2:2814137/148‑1 (MQ=255)
                                           tACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTtctc                                                                                                         >  1:448350/1‑148 (MQ=255)
                                            aCTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCg                                                                                                        >  1:778278/1‑148 (MQ=255)
                                             cTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGt                                                                                                       <  1:2345918/148‑1 (MQ=255)
                                             cTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGt                                                                                                       <  1:202735/148‑1 (MQ=255)
                                                ttttCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTc                                                                                                    >  2:1632646/1‑148 (MQ=255)
                                                ttttCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTc                                                                                                    >  1:2104814/1‑148 (MQ=255)
                                                  ttCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGc                                                                                                  <  2:96301/148‑1 (MQ=255)
                                                     cGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTtg                                                                                               <  1:58675/148‑1 (MQ=255)
                                                       cccTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTtgct                                                                                             <  2:2522505/148‑1 (MQ=255)
                                                         cTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTtgctgc                                                                                           >  1:1279214/1‑148 (MQ=255)
                                                           gTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGt                                                                                         <  2:923884/148‑1 (MQ=255)
                                                           gTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAAAAATACGCCCGGACTTGAAGACATTTTACGCTTTCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGt                                                                                         >  2:1062081/1‑148 (MQ=255)
                                                            ttAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTc                                                                                        >  2:1160463/1‑148 (MQ=255)
                                                            ttAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTc                                                                                        >  2:561591/1‑148 (MQ=255)
                                                            ttAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCAGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTc                                                                                        >  2:1160465/1‑148 (MQ=255)
                                                             tAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTcc                                                                                       >  2:2613408/1‑148 (MQ=255)
                                                               gCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGc                                                                                     >  2:2594231/1‑148 (MQ=255)
                                                               gCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGc                                                                                     <  2:3043948/148‑1 (MQ=255)
                                                                ccGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCt                                                                                    <  1:2486198/148‑1 (MQ=255)
                                                                 cGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCtt                                                                                   <  2:3281736/148‑1 (MQ=255)
                                                                   cagcaAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTgg                                                                                 >  2:1933365/1‑148 (MQ=255)
                                                                   cagcaAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTgg                                                                                 <  2:2949238/148‑1 (MQ=255)
                                                                   cagcaAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATACTTTCTCGTTTCGCTTGCTGCGTCCGCTTgg                                                                                 >  1:932244/1‑148 (MQ=255)
                                                                    agcaAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGt                                                                                <  2:1904671/148‑1 (MQ=255)
                                                                      caAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTta                                                                              >  2:1092306/1‑148 (MQ=255)
                                                                             tGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAAAACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCAcc                                                                       >  1:840269/1‑148 (MQ=255)
                                                                             tGGCGTTCGCGTCCGTTTGTTGCCGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCAcc                                                                       >  2:656159/1‑148 (MQ=255)
                                                                               gCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGa                                                                     <  2:2699650/148‑1 (MQ=255)
                                                                                cGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGAc                                                                    >  2:1405152/1‑148 (MQ=255)
                                                                                 gTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACt                                                                   <  2:3025726/148‑1 (MQ=255)
                                                                                  ttCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACtt                                                                  <  1:2189551/148‑1 (MQ=255)
                                                                                   tCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACttt                                                                 >  1:3069535/1‑148 (MQ=255)
                                                                                   tCGCGTCCGTTTGTTGCAGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCGTGAAAGTCATCCACCCAATGAAGTAAGGCTTTTTAAACCTTTCACGTTTCGCTTGCTGCGTCCGCTTGGTTATATAAACGACttt                                                                 >  1:3071314/1‑148 (MQ=255)
                                                                                      cgTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGACGTCCGACTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTcc                                                              >  1:2654355/1‑148 (MQ=255)
                                                                                      cgTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGAAGTCCGACTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTcc                                                              >  1:404040/1‑148 (MQ=255)
                                                                                      cgTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTcc                                                              >  1:1601375/1‑148 (MQ=255)
                                                                                         ccGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGt                                                           >  1:1703479/1‑148 (MQ=255)
                                                                                          cGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTc                                                          <  1:1035323/148‑1 (MQ=255)
                                                                                           gTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCt                                                         >  1:950841/1‑148 (MQ=255)
                                                                                           gTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCt                                                         <  2:786657/148‑1 (MQ=255)
                                                                                             ttgttgCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTa                                                       <  2:1698222/148‑1 (MQ=255)
                                                                                             ttgttgCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTa                                                       <  2:2160114/148‑1 (MQ=255)
                                                                                             ttgttgCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTa                                                       >  1:1167574/1‑148 (MQ=255)
                                                                                             ttgttgCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTa                                                       >  2:1198112/1‑148 (MQ=255)
                                                                                               gttgCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAAt                                                     <  1:3076803/148‑1 (MQ=255)
                                                                                                     ggACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGt                                               <  1:486572/148‑1 (MQ=255)
                                                                                                      gacgacAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTa                                              >  1:2077043/1‑148 (MQ=255)
                                                                                                       acgacAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTAt                                             >  1:1073453/1‑148 (MQ=255)
                                                                                                          acaacaATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGca                                          >  2:2900421/1‑148 (MQ=255)
                                                                                                            aacaaTACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGcaca                                        <  2:2722022/148‑1 (MQ=255)
                                                                                                             acaaTACGCCCGGACTTGACGACATTTTACGCTTGCTTTACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACaa                                       >  1:1050607/1‑148 (MQ=255)
                                                                                                             acaaTACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACaa                                       >  1:34560/1‑148 (MQ=255)
                                                                                                                aTACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATaa                                    >  2:492230/1‑148 (MQ=255)
                                                                                                                 tACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATaaa                                   >  2:3173363/1‑148 (MQ=255)
                                                                                                                  aCGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATaaaa                                  >  2:537569/1‑148 (MQ=255)
                                                                                                                  aCGCCCGGACTAGACGACATTTTACGCTTGCATGACAGTCATCCACGCATTGAAGTCCGGCTATTTAATCCTTTCTCGTTTCTCTTGCTGCGTCCGCTTGGTTATATCACCGACAATTCCCGTCTTAATCGCCCTATGCACAATaaaa                                  >  2:1274115/1‑148 (MQ=255)
                                                                                                                     cccGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGtt                               >  2:624496/1‑148 (MQ=255)
                                                                                                                       cGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTc                             <  2:933781/148‑1 (MQ=255)
                                                                                                                        ggACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCa                            <  1:2900421/148‑1 (MQ=255)
                                                                                                                         gACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCAc                           <  1:1756999/148‑1 (MQ=255)
                                                                                                                               acgacATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGa                     <  1:573789/148‑1 (MQ=255)
                                                                                                                               acgacATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGa                     >  2:1612066/1‑148 (MQ=255)
                                                                                                                               acgacATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGa                     <  1:1611444/148‑1 (MQ=255)
                                                                                                                                     ttttACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCgt               >  2:995403/1‑148 (MQ=255)
                                                                                                                                      tttACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCgtg              <  1:2981365/148‑1 (MQ=255)
                                                                                                                                       ttACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCgtgg             <  2:980673/148‑1 (MQ=255)
                                                                                                                                        tACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCgtggt            <  2:840269/148‑1 (MQ=255)
                                                                                                                                         aCGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCNTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCgtggtg           <  1:1682254/148‑1 (MQ=255)
                                                                                                                                          cGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGa          >  1:1729818/1‑148 (MQ=255)
                                                                                                                                            cttgcttgACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGAcc        <  2:682361/148‑1 (MQ=255)
                                                                                                                                            cttgcttgACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGAcc        <  1:2269676/148‑1 (MQ=255)
                                                                                                                                              tgcttgACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGACCCt      >  2:1993514/1‑148 (MQ=255)
                                                                                                                                              tgcttgACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGACCCt      >  2:2919076/1‑148 (MQ=255)
                                                                                                                                                  tgACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGACCCtggtg  >  1:198814/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGTTCAGTATTACATCTGGCAGGACGATATGTCGGGTCGGTTACTGTTTTCCGCCCTGTTAGCCGCAGCAAAGCGTGGCGTTCGCGTCCGTTTGTTGCTGGACGACAACAATACGCCCGGACTTGACGACATTTTACGCTTGCTTGACAGTCATCCACGCATTGAAGTCCGGCTTTTTAATCCTTTCTCGTTTCGCTTGCTGCGTCCGCTTGGTTATATCACCGACTTTTCCCGTCTTAATCGCCGTATGCACAATAAAAGTTTCACTGTCGATGGCGTGGTGACCCTGGTG  >  CP009273/1101946‑1102239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑