Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 104,561 G→T 100% A124S (GCG→TCG)  secM → regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273104,5610GT100.0% 595.5 / NA 176A124S (GCG→TCG) secMregulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (105/71);  total (105/71)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

CTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACC  >  CP009273/104416‑104701
                                                                                                                                                 |                                                                                                                                            
cTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAgctcg                                                                                                                                            <  2:2813675/148‑1 (MQ=255)
cTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAgctcg                                                                                                                                            >  1:2839281/1‑148 (MQ=255)
   ttCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCgctg                                                                                                                                         >  2:1007896/1‑148 (MQ=255)
       gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                     >  2:148034/1‑148 (MQ=255)
       gTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCAGTACTGGATACGCTCAGCTCGCTGCTGa                                                                                                                                     >  2:1819764/1‑148 (MQ=255)
        ttGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGAc                                                                                                                                    >  1:296548/1‑148 (MQ=255)
             tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAgg                                                                                                                               >  2:1194945/1‑148 (MQ=255)
             tACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAgg                                                                                                                               >  1:1171595/1‑148 (MQ=255)
              aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGa                                                                                                                              >  1:870588/1‑148 (MQ=255)
              aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGa                                                                                                                              >  1:870560/1‑148 (MQ=255)
              aCTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGa                                                                                                                              <  1:1893853/148‑1 (MQ=255)
               cTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCTCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGaa                                                                                                                             >  2:2240818/1‑148 (MQ=255)
               cTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGaa                                                                                                                             <  2:1194543/148‑1 (MQ=255)
                 ggCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAgg                                                                                                                           >  1:3266873/1‑148 (MQ=255)
                  gCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGc                                                                                                                          <  1:1320054/148‑1 (MQ=255)
                  gCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGc                                                                                                                          <  2:2371075/148‑1 (MQ=255)
                     tCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACg                                                                                                                       >  1:2294189/1‑148 (MQ=255)
                     tCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACg                                                                                                                       <  2:3068674/148‑1 (MQ=255)
                      cAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGc                                                                                                                      <  1:234804/148‑1 (MQ=255)
                         cATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGt                                                                                                                   >  2:2935352/1‑148 (MQ=255)
                         cATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGt                                                                                                                   >  2:1767387/1‑148 (MQ=255)
                         cATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGt                                                                                                                   >  1:2296938/1‑148 (MQ=255)
                         cATGCCATTCGCACGGTAATCCATCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGt                                                                                                                   >  1:3216449/1‑148 (MQ=255)
                            gCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTg                                                                                                                >  2:2062281/1‑148 (MQ=255)
                              cATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaa                                                                                                              >  2:927069/1‑148 (MQ=255)
                               agtCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                             <  2:1900742/146‑1 (MQ=255)
                               aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                             >  1:2181775/1‑148 (MQ=255)
                               aTTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaa                                                                                                             <  2:358765/148‑1 (MQ=255)
                                ttCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaaa                                                                                                            >  2:339928/1‑148 (MQ=255)
                                ttCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGaaaa                                                                                                            <  2:1170873/148‑1 (MQ=255)
                                    cACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGt                                                                                                        >  1:1352616/1‑148 (MQ=255)
                                       ggTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                     <  1:173874/148‑1 (MQ=255)
                                       ggTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                     <  2:1685400/148‑1 (MQ=255)
                                       ggTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTAt                                                                                                     <  2:1685189/148‑1 (MQ=255)
                                         tAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCg                                                                                                   >  2:2575886/1‑148 (MQ=255)
                                          aaTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGc                                                                                                  <  2:617081/148‑1 (MQ=255)
                                           aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCa                                                                                                 <  1:2901600/148‑1 (MQ=255)
                                           aTCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCa                                                                                                 <  1:1251305/148‑1 (MQ=255)
                                            tCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                >  2:640476/1‑148 (MQ=255)
                                            tCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                >  1:1761938/1‑148 (MQ=255)
                                            tCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                <  1:699491/148‑1 (MQ=255)
                                            tCCGTCATCTTTCGTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAt                                                                                                <  1:2266717/148‑1 (MQ=255)
                                             ccGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                               <  1:1919869/148‑1 (MQ=255)
                                             ccGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCAtt                                                                                               >  1:2656750/1‑148 (MQ=255)
                                              cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTg                                                                                              <  2:2818822/148‑1 (MQ=255)
                                              cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTg                                                                                              <  2:890444/148‑1 (MQ=255)
                                              cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTg                                                                                              <  1:245346/148‑1 (MQ=255)
                                              cGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTg                                                                                              <  2:2285873/148‑1 (MQ=255)
                                                tCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGAt                                                                                            <  1:2750115/148‑1 (MQ=255)
                                                  aTCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTa                                                                                          >  2:134786/1‑148 (MQ=255)
                                                   tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGCAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                         >  2:2004407/1‑148 (MQ=255)
                                                   tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                         >  1:3204243/1‑148 (MQ=255)
                                                   tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                         >  1:3204300/1‑148 (MQ=255)
                                                   tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                         >  2:1097022/1‑148 (MQ=255)
                                                   tctttcttTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTAt                                                                                         >  1:2514486/1‑148 (MQ=255)
                                                         tttcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCGTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCAt                                                                                   <  1:2730431/148‑1 (MQ=255)
                                                           tcGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCAttt                                                                                 <  1:972572/148‑1 (MQ=255)
                                                             gCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTa                                                                               <  1:1544220/148‑1 (MQ=255)
                                                                aTGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTAccc                                                                            <  1:1023884/148‑1 (MQ=255)
                                                                   gCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCcac                                                                         >  1:1052418/1‑148 (MQ=255)
                                                                   gCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCcac                                                                         >  2:2423137/1‑148 (MQ=255)
                                                                     aCCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACaa                                                                       >  1:514647/1‑148 (MQ=255)
                                                                         cAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaa                                                                   >  2:275075/1‑148 (MQ=255)
                                                                          aaaCACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaa                                                                  >  2:3056804/1‑148 (MQ=255)
                                                                           aaCACTGCCCGTTGCTGAAGAATCTGTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCaaaa                                                                 <  2:2880568/148‑1 (MQ=255)
                                                                              acTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATGGATTATGCGCATTTTACCCCACCAGCAAAATTc                                                              >  1:151072/1‑148 (MQ=255)
                                                                               cTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCa                                                             <  2:1406136/148‑1 (MQ=255)
                                                                                tGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAg                                                            <  1:1118783/148‑1 (MQ=255)
                                                                                 gCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGc                                                           <  1:2136425/148‑1 (MQ=255)
                                                                                 gCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGc                                                           >  2:2903873/1‑148 (MQ=255)
                                                                                  cccGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCa                                                          >  1:2064235/1‑148 (MQ=255)
                                                                                           gaagaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGACt                                                 >  1:1705918/1‑148 (MQ=255)
                                                                                            aagaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTg                                                >  2:1579224/1‑148 (MQ=255)
                                                                                              gaaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGa                                              >  1:2301599/1‑148 (MQ=255)
                                                                                               aaTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGAt                                             >  2:1060975/1‑148 (MQ=255)
                                                                                                aTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATa                                            >  2:2247239/1‑148 (MQ=255)
                                                                                                aTCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATa                                            >  2:2681031/1‑148 (MQ=255)
                                                                                                 tCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATGTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATaa                                           >  1:8068/1‑148 (MQ=255)
                                                                                                  cTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAg                                          >  2:650060/1‑148 (MQ=255)
                                                                                                     tGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCa                                       <  1:3236774/148‑1 (MQ=255)
                                                                                                       ccTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAgg                                     >  2:1028382/1‑148 (MQ=255)
                                                                                                        ctctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGgc                                    >  2:2498326/1‑148 (MQ=255)
                                                                                                          ctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGgcgc                                  >  2:383175/1‑148 (MQ=255)
                                                                                                          ctTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGgcgc                                  >  2:1558110/1‑148 (MQ=255)
                                                                                                            tCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCaa                                >  2:2264803/1‑148 (MQ=255)
                                                                                                                gcgcAACATCTTGCGTTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTATGGATAAGCCAGGCGCAAGGCa                            <  1:942622/148‑1 (MQ=255)
                                                                                                                gcgcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCa                            >  1:822916/1‑148 (MQ=255)
                                                                                                                  gcAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATc                          <  2:1638179/148‑1 (MQ=255)
                                                                                                                   cAACATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATcc                         >  2:2764895/1‑148 (MQ=255)
                                                                                                                      cATCTTGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCTTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAATCCAGGCGCAAGGCATCCGTg                      >  1:2774501/1‑148 (MQ=255)
                                                                                                                           tGCATTACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGc                 >  1:3109978/1‑148 (MQ=255)
                                                                                                                               ttACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTc             >  1:936726/1‑148 (MQ=255)
                                                                                                                                tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCa            >  2:1393167/1‑148 (MQ=255)
                                                                                                                                tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCTCGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCa            >  1:721174/1‑148 (MQ=255)
                                                                                                                                tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCa            <  1:2288614/148‑1 (MQ=255)
                                                                                                                                tACTGGATACGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCa            <  1:412808/148‑1 (MQ=255)
                                                                                                                                         cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAc   <  1:233588/148‑1 (MQ=255)
                                                                                                                                         cGCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAc   <  2:3109978/148‑1 (MQ=255)
                                                                                                                                          gCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAcc  <  2:3299023/148‑1 (MQ=255)
                                                                                                                                          gCTCAGCTCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCAcc  <  2:963739/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CTATTCCGTTGATTACTGGCATCAACATGCCATTCGCACGGTAATCCGTCATCTTTCTTTCGCAATGGCACCGCAAACACTGCCCGTTGCTGAAGAATCTTTGCCTCTTCAGGCGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTCTGAAAAGGGTTATCGCATTGATTATGCGCATTTTACCCCACAAGCAAAATTCAGCACGCCCGTCTGGATAAGCCAGGCGCAAGGCATCCGTGCTGGCCCTCAACGCCTCACC  >  CP009273/104416‑104701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑