Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,163,412 G→T 16.1% D120Y (GAT→TAT)  ycfJ → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,163,4120GT16.1% 480.7 / 49.0 162D120Y (GAT→TAT) ycfJuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (52/83);  new base T (14/13);  total (66/96)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGAT  >  CP009273/1163266‑1163558
                                                                                                                                                  |                                                                                                                                                  
ttACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGACAGCGa                                                                                                                                                   >  2:2401882/1‑148 (MQ=255)
ttACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGa                                                                                                                                                   >  2:2401978/1‑148 (MQ=255)
ttACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGGTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGa                                                                                                                                                   >  1:25397/1‑148 (MQ=255)
 tACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGAGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGAt                                                                                                                                                  <  1:494652/148‑1 (MQ=255)
    cGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACt                                                                                                                                               <  2:716729/148‑1 (MQ=255)
    cGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACt                                                                                                                                               <  2:2841433/148‑1 (MQ=255)
    cGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACt                                                                                                                                               <  2:2692864/148‑1 (MQ=255)
     gggTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACtt                                                                                                                                              <  1:524369/148‑1 (MQ=255)
     gggTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACtt                                                                                                                                              <  1:524396/148‑1 (MQ=255)
      ggTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTa                                                                                                                                             >  1:2201430/1‑148 (MQ=255)
         cGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTacac                                                                                                                                          >  1:1055757/1‑148 (MQ=255)
           gTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGa                                                                                                                                        <  2:729762/148‑1 (MQ=255)
             gCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACt                                                                                                                                      <  2:2814296/148‑1 (MQ=255)
             gCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACt                                                                                                                                      <  1:1755496/148‑1 (MQ=255)
             gCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACt                                                                                                                                      >  1:3033452/1‑148 (MQ=255)
              cTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTa                                                                                                                                     >  1:773435/1‑148 (MQ=255)
                cGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACg                                                                                                                                   <  2:1965907/148‑1 (MQ=255)
                  gcgcTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCa                                                                                                                                 <  2:1032015/148‑1 (MQ=255)
                   cgcTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCaa                                                                                                                                <  1:1616378/148‑1 (MQ=255)
                   cgcTGTTGCGGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCaa                                                                                                                                <  1:19393/148‑1 (MQ=255)
                     cTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACa                                                                                                                              <  2:387724/148‑1 (MQ=255)
                     cTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGCGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACa                                                                                                                              <  2:2052797/148‑1 (MQ=255)
                          gCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGtt                                                                                                                         <  2:2655072/148‑1 (MQ=255)
                               cggcgTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTaaa                                                                                                                    <  2:121792/148‑1 (MQ=255)
                                  cgTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACg                                                                                                                 <  2:2666262/148‑1 (MQ=255)
                                    tGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGAGGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGgt                                                                                                               <  1:1949605/148‑1 (MQ=255)
                                      aTAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGgtgt                                                                                                             <  1:2286703/148‑1 (MQ=255)
                                      aTAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGgtgt                                                                                                             >  1:3206092/1‑148 (MQ=255)
                                      aTAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGgtgt                                                                                                             >  1:3012741/1‑148 (MQ=255)
                                       tAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTa                                                                                                            <  1:2052640/148‑1 (MQ=255)
                                         gggCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATg                                                                                                          <  2:1002872/148‑1 (MQ=255)
                                          ggCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGa                                                                                                         <  1:2085394/148‑1 (MQ=255)
                                            cATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACa                                                                                                       >  1:964748/1‑148 (MQ=255)
                                            cATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACa                                                                                                       <  2:3068460/148‑1 (MQ=255)
                                             aTCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACaa                                                                                                      <  1:2721509/148‑1 (MQ=255)
                                                  tttGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAg                                                                                                 >  2:45690/1‑148 (MQ=255)
                                                  tttGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAg                                                                                                 >  1:1058897/1‑148 (MQ=255)
                                                       tggtggtCTCGGTAAAGATGTCGCCAGTGTTGTGGGGGCGCTGGGTGGTGTATATGCCGGTATCCAGATCCAGGGCTCTCGCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaaa                                                                                            <  1:1010189/148‑1 (MQ=255)
                                                            gtCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCt                                                                                       <  2:1203542/148‑1 (MQ=255)
                                                                cgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGt                                                                                   >  2:2183164/1‑148 (MQ=255)
                                                                  gTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTa                                                                                 >  2:1778234/1‑148 (MQ=255)
                                                                  gTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTa                                                                                 >  2:1777564/1‑148 (MQ=255)
                                                                    aaaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatg                                                                               <  2:2704018/148‑1 (MQ=255)
                                                                    aaaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatg                                                                               <  2:1175229/148‑1 (MQ=255)
                                                                    aaaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatg                                                                               <  2:1175141/148‑1 (MQ=255)
                                                                      aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgat                                                                             >  1:892709/1‑148 (MQ=255)
                                                                      aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgat                                                                             >  1:893383/1‑148 (MQ=255)
                                                                             gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt                                                                      <  2:2644880/148‑1 (MQ=255)
                                                                             gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt                                                                      <  1:240120/148‑1 (MQ=255)
                                                                               cACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtat                                                                    >  2:2867089/1‑148 (MQ=255)
                                                                                aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtata                                                                   <  1:3227938/148‑1 (MQ=255)
                                                                                aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtata                                                                   <  1:1322551/148‑1 (MQ=255)
                                                                                aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtata                                                                   >  1:2672232/1‑148 (MQ=255)
                                                                                  tgttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg                                                                 <  1:2500305/148‑1 (MQ=255)
                                                                                   gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGa                                                                >  1:2519351/1‑148 (MQ=255)
                                                                                   gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGa                                                                <  1:1825991/148‑1 (MQ=255)
                                                                                   gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGa                                                                <  1:155006/148‑1 (MQ=255)
                                                                                      gtgGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg                                                             <  2:598869/148‑1 (MQ=255)
                                                                                           ggCGCTGGGTGTTTGATATGCCGGTAACCACATCCAGGTCATTCTCCAGGAAACCGATACTTACACGACTACGCAACAGCGTTGTAACACGGTGTATGACCAGTCAGAAAAAATGATCGGTTATGATGTGACCTATAAGATTGGCGAt                                                        <  1:893559/148‑1 (MQ=255)
                                                                                               cTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATcagc                                                    <  2:1254005/148‑1 (MQ=255)
                                                                                                 gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATcagcag                                                  <  1:1865265/148‑1 (MQ=255)
                                                                                                 gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATcagcag                                                  <  1:1473349/148‑1 (MQ=255)
                                                                                                  ggtggtGGATATGCCGGTAACCAGATCCATGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAgg                                                 <  1:1769673/148‑1 (MQ=255)
                                                                                                  ggtggtGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATAC‑TACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAggg                                                >  2:466076/1‑148 (MQ=255)
                                                                                                  ggtggtGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAgg                                                 >  1:3018953/1‑148 (MQ=255)
                                                                                                       tggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTTTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCaaa                                            <  2:1677087/148‑1 (MQ=255)
                                                                                                       tggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCaaa                                            <  1:3298781/148‑1 (MQ=255)
                                                                                                        ggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCaaaa                                           <  1:2800489/148‑1 (MQ=255)
                                                                                                         gATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAAt                                          >  2:2218576/1‑148 (MQ=255)
                                                                                                          atatGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATc                                         <  1:1667784/148‑1 (MQ=255)
                                                                                                            atGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCg                                       >  2:2632832/1‑148 (MQ=255)
                                                                                                             tGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGc                                      <  1:1152749/148‑1 (MQ=255)
                                                                                                                cGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATg                                   <  2:3271974/148‑1 (MQ=255)
                                                                                                                 ggTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATgg                                  <  2:964748/148‑1 (MQ=255)
                                                                                                                  gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGa                                 >  1:1025591/1‑148 (MQ=255)
                                                                                                                  gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGa                                 <  2:233791/148‑1 (MQ=255)
                                                                                                                      ccAGATCCAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACcgc                             >  2:2533625/1‑148 (MQ=255)
                                                                                                                      ccAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACcgc                             >  2:3127555/1‑148 (MQ=255)
                                                                                                                       cAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACcgcg                            >  2:2305382/1‑148 (MQ=255)
                                                                                                                            ccAGGGCTCTCTCCAGGAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCg                       <  2:667259/148‑1 (MQ=255)
                                                                                                                            ccAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCg                       <  2:3206092/148‑1 (MQ=255)
                                                                                                                            ccAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCg                       <  2:3012741/148‑1 (MQ=255)
                                                                                                                              aGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCggg                     >  1:83403/1‑148 (MQ=255)
                                                                                                                                ggCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTa                   >  1:335823/1‑148 (MQ=255)
                                                                                                                                ggCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTa                   >  1:336243/1‑148 (MQ=255)
                                                                                                                                   tctctcCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGc                <  2:1058897/148‑1 (MQ=255)
                                                                                                                                        ccAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATc           <  1:2709924/148‑1 (MQ=255)
                                                                                                                                        ccAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATc           <  1:183911/148‑1 (MQ=255)
                                                                                                                                          aGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATccc         >  2:1611487/1‑148 (MQ=255)
                                                                                                                                          aGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATccc         >  2:1611505/1‑148 (MQ=255)
                                                                                                                                           ggAAAGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg        <  1:2460796/148‑1 (MQ=255)
                                                                                                                                           ggAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg        <  1:997830/148‑1 (MQ=255)
                                                                                                                                           ggAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg        >  2:2648118/1‑148 (MQ=255)
                                                                                                                                           ggAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCg        <  1:2651260/148‑1 (MQ=255)
                                                                                                                                            gAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGc       <  2:3076851/148‑1 (MQ=255)
                                                                                                                                            gAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGc       >  2:2198485/1‑148 (MQ=255)
                                                                                                                                               aGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAg    <  1:2879089/148‑1 (MQ=255)
                                                                                                                                               aGCTATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAg    <  1:2879072/148‑1 (MQ=255)
                                                                                                                                                gCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGa   <  2:2035997/148‑1 (MQ=255)
                                                                                                                                                 cGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGAt  >  2:151385/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTACCGGGTCGGTGCTCGGCGCTGTTGCTGGCGGCGTGATAGGGCATCAGTTTGGTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATCAGCAGGGCAAAATCCGCATGGACCGCGATCCGGGTACGCAGATCCCGCTAGAT  >  CP009273/1163266‑1163558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑