Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,237,168 A→T 7.3% I120I (ATT→ATA)  cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,237,1680AT 7.3% 492.6 / 24.0 163I120I (ATT→ATA) cvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (88/63);  new base T (8/4);  total (96/67)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 186 total aligned reads displayed.

CGTCAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATA  >  CP009273/1237021‑1237315
                                                                                                                                                   |                                                                                                                                                   
cGTCAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTtca                                                                                                                                                     <  2:2488592/148‑1 (MQ=255)
   cAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTtcaatc                                                                                                                                                  >  1:932792/1‑148 (MQ=255)
   cAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTtcaatc                                                                                                                                                  >  1:3138394/1‑148 (MQ=255)
   cAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTtcaatc                                                                                                                                                  >  1:155466/1‑148 (MQ=255)
     gAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTtcaatcaa                                                                                                                                                <  2:1331426/148‑1 (MQ=255)
      aaaGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCaatcaaa                                                                                                                                               >  1:255283/1‑148 (MQ=255)
      aaaGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCaatcaaa                                                                                                                                               <  2:1452533/148‑1 (MQ=255)
         gACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCaatcaaatca                                                                                                                                            <  2:1430146/148‑1 (MQ=255)
          aCCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCTatcaaatcaa                                                                                                                                           <  2:1837320/148‑1 (MQ=255)
           ccGCAATTGGATAATTAGTGCCTGTTTCAATTTGCAGCGTCGAGCAAACACGTTATTTAAGCCCATTACCACCATGCAGATAAAAGACCGCTGCAGCATCGGTAGAGCCGACTATAGCGCCGATTAATAAGCCTTCAATCAAATCAAg                                                                                                                                          <  1:6068/148‑1 (MQ=255)
            cGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGa                                                                                                                                         <  1:498202/148‑1 (MQ=255)
              ccATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCTATCAAATCAAGAtt                                                                                                                                       >  1:2578581/1‑148 (MQ=255)
              ccATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGAtt                                                                                                                                       >  1:2904691/1‑148 (MQ=255)
               cATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTa                                                                                                                                      >  2:3229520/1‑148 (MQ=255)
                 ttGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTaaa                                                                                                                                    >  1:1121320/1‑148 (MQ=255)
                     aTCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGc                                                                                                                                <  1:3204356/148‑1 (MQ=255)
                     aTCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGc                                                                                                                                <  1:1565461/148‑1 (MQ=255)
                     aTCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGc                                                                                                                                <  1:1665289/148‑1 (MQ=255)
                      tCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGcc                                                                                                                               >  2:1470472/1‑148 (MQ=255)
                       cATTACTGCCGTATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCa                                                                                                                              <  2:1426468/148‑1 (MQ=255)
                       cATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCa                                                                                                                              >  2:655147/1‑148 (MQ=255)
                              gCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACgccgcc                                                                                                                       >  2:263345/1‑148 (MQ=255)
                              gCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACgccgcc                                                                                                                       <  2:706716/148‑1 (MQ=255)
                              gCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACgccgcc                                                                                                                       >  1:3100162/1‑148 (MQ=255)
                               ccGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCca                                                                                                                      <  2:1007902/148‑1 (MQ=255)
                                  gATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCcatca                                                                                                                   >  1:474179/1‑148 (MQ=255)
                                     tCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCTATCAAATCAAGATTAAACAGCCACGCCGCCATCATga                                                                                                                >  2:2565308/1‑147 (MQ=255)
                                     tCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGc                                                                                                                >  1:377000/1‑148 (MQ=255)
                                      cAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGcc                                                                                                               >  1:723407/1‑148 (MQ=255)
                                      cAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGcc                                                                                                               >  1:723405/1‑148 (MQ=255)
                                        aTTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCTATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCgg                                                                                                             >  2:1277952/1‑148 (MQ=255)
                                        aTTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCgg                                                                                                             >  2:2837261/1‑148 (MQ=255)
                                         tttCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGt                                                                                                            <  2:1612638/148‑1 (MQ=255)
                                         tttCCAGCGTCGACCCAACACGTTCGTTAAGCCCCTTACCACCCACCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGt                                                                                                            <  1:946692/148‑1 (MQ=255)
                                               gCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAAcc                                                                                                      <  2:1023391/148‑1 (MQ=255)
                                               gCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAAcc                                                                                                      >  1:447659/1‑148 (MQ=255)
                                                 gTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCag                                                                                                    <  1:2944739/148‑1 (MQ=255)
                                                        cAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGAt                                                                                             >  1:1432798/1‑148 (MQ=255)
                                                        cAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGAt                                                                                             <  1:2900581/148‑1 (MQ=255)
                                                         aaCACGTTCTTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATa                                                                                            <  1:1523446/148‑1 (MQ=255)
                                                          acacGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATaa                                                                                           >  2:2984289/1‑148 (MQ=255)
                                                          acacGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATaa                                                                                           >  2:3200488/1‑148 (MQ=255)
                                                           cacGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAg                                                                                          >  2:2684568/1‑148 (MQ=255)
                                                           cacGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAg                                                                                          >  1:791755/1‑148 (MQ=255)
                                                           cacGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAg                                                                                          >  1:851946/1‑148 (MQ=255)
                                                            acGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGc                                                                                         >  1:2477868/1‑148 (MQ=255)
                                                            acGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGc                                                                                         >  1:802554/1‑148 (MQ=255)
                                                              gttcgttAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCAc                                                                                       >  2:970429/1‑148 (MQ=255)
                                                                tcgttAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGc                                                                                     >  1:2135574/1‑148 (MQ=255)
                                                                 cgttAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGcc                                                                                    <  1:2748650/148‑1 (MQ=255)
                                                                  gttAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGccc                                                                                   <  1:1269317/148‑1 (MQ=255)
                                                                       gCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGt                                                                              >  2:478708/1‑148 (MQ=255)
                                                                       gCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGt                                                                              >  1:1377453/1‑148 (MQ=255)
                                                                        ccccTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTc                                                                             >  2:1350524/1‑148 (MQ=255)
                                                                          ccTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc                                                                           <  1:750710/148‑1 (MQ=255)
                                                                          ccTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGc                                                                           >  2:3289505/1‑148 (MQ=255)
                                                                            ttACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCa                                                                         >  2:2777206/1‑148 (MQ=255)
                                                                                   ccAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAg                                                                  <  1:2705224/148‑1 (MQ=255)
                                                                                   ccAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAg                                                                  <  1:1610805/148‑1 (MQ=255)
                                                                                   ccAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAg                                                                  <  1:1367772/148‑1 (MQ=255)
                                                                                   ccAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAACCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAg                                                                  <  1:2705957/148‑1 (MQ=255)
                                                                                     agcagAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTg                                                                <  2:1373553/148‑1 (MQ=255)
                                                                                         gagaAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCTATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCgg                                                            >  1:3034741/1‑148 (MQ=255)
                                                                                         gagaAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCgg                                                            >  2:3017181/1‑148 (MQ=255)
                                                                                         gagaAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCgg                                                            >  2:2146027/1‑148 (MQ=255)
                                                                                           gaAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTc                                                          >  2:1468183/1‑148 (MQ=255)
                                                                                           gaAAAGACCGCGGCAGCAGCGGTAGAGCCGACGATAGCGCCGATTAATAACCCTTCTAACAAATCAAGATTAAACCGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTc                                                          <  1:32233/148‑1 (MQ=255)
                                                                                            aaaaGACCGCTGCAGCATCTGTAGAGCCGACGATAGCGCGGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTcc                                                         <  2:2085920/148‑1 (MQ=255)
                                                                                            aaaaGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACCGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTcc                                                         <  1:162941/148‑1 (MQ=255)
                                                                                            aaaaGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTcc                                                         <  1:1833791/148‑1 (MQ=255)
                                                                                               aGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTaa                                                      >  1:3267106/1‑148 (MQ=255)
                                                                                                gACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATTCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACCCTGCCGGTCCTAAc                                                     >  1:2283211/1‑148 (MQ=255)
                                                                                                     cTGCAGCTTGGGTAGAGCCGACGTTAGCGCCGATTAATAAGACTTAAAACAAATAAAGATAAAACAGCCACGCGGCCATCATGCCGGTTAAACCAGAGGTTATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                <  1:19447/148‑1 (MQ=255)
                                                                                                     cTGCAGCAGCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                <  1:2538117/148‑1 (MQ=255)
                                                                                                           caTCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGaaa                                          <  2:1613482/148‑1 (MQ=255)
                                                                                                             tCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAgg                                        >  2:2376287/1‑148 (MQ=255)
                                                                                                             tCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAgg                                        >  2:422148/1‑148 (MQ=255)
                                                                                                             tCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAgg                                        >  2:422211/1‑148 (MQ=255)
                                                                                                              cGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                       <  1:2393460/148‑1 (MQ=255)
                                                                                                              cGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                       >  1:1742736/1‑148 (MQ=255)
                                                                                                               ggTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGaa                                      <  1:2058900/148‑1 (MQ=255)
                                                                                                                 tAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACt                                    >  2:798143/1‑148 (MQ=255)
                                                                                                                     gCCGACGATAGCGCCGATTAATAAGCCTTCTATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGcc                                >  1:3236775/1‑148 (MQ=255)
                                                                                                                        gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGa                             >  2:54000/1‑148 (MQ=255)
                                                                                                                         acgaTATCGCAGATTAATTAGCCTTCATTCAAATCTAGATTAAACAGCCAGGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCTCGCCCAGCGTCGGCAGCTACATTGCCGGTCCTAACGCAACACGTAAGGAACTGGCCTGAg                            <  2:1648753/148‑1 (MQ=255)
                                                                                                                           gaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTg                          <  2:3041532/148‑1 (MQ=255)
                                                                                                                             tAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTgcg                        >  2:1072194/1‑148 (MQ=255)
                                                                                                                                cgcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCCGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCAt                     >  2:1767730/1‑148 (MQ=255)
                                                                                                                                cgcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCAt                     >  1:1262391/1‑148 (MQ=255)
                                                                                                                                       taataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgccg              >  2:1177307/1‑148 (MQ=255)
                                                                                                                                          taaGCCTTCTATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCg           >  2:844789/1‑148 (MQ=255)
                                                                                                                                          taaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCg           >  2:861114/1‑148 (MQ=255)
                                                                                                                                               cTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAGGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaa      >  1:2219354/1‑148 (MQ=255)
                                                                                                                                                ttCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaaa     >  2:1897178/1‑148 (MQ=255)
                                                                                                                                                 tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCg           >  2:2705522/1‑141 (MQ=255)
                                                                                                                                                 tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaaaa    >  2:1619494/1‑148 (MQ=255)
                                                                                                                                                 tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaaaa    >  2:1718759/1‑148 (MQ=255)
                                                                                                                                                 tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaaaa    >  2:1995963/1‑148 (MQ=255)
                                                                                                                                                 tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCaaaa    >  2:1995895/1‑148 (MQ=255)
                                                                                                                                                   aatcaaatcaAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAaata  <  2:2913521/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTCAGAAAGACCGCCATTGGATCATTACTGCCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATA  >  CP009273/1237021‑1237315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑