Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,227 G→T 90.2% R728S (CGT→AGT)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,2270GT90.2% 496.8 / 47.8 193R728S (CGT→AGT) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (13/6);  new base T (81/93);  total (94/99)
Fisher's exact test for biased strand distribution p-value = 9.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 193 total aligned reads displayed.

GTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249084‑1249370
                                                                                                                                               |                                                                                                                                               
gTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTa                                                                                                                                             <  2:1212955/148‑1 (MQ=255)
 tAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAt                                                                                                                                            >  1:2597027/1‑148 (MQ=255)
  attatcggTTTTGTGCGATTTAACTGTCGTACATTACCATGTTGTCTGTAAGTGCGGTACGGTCGACTCCGCTATAGTCCTGCGATGCATGTGTTGATCCAATATACAGACCGACATACAACGGCATTACATCAGAGTGACTTTTATc                                                                                                                                           <  2:1436722/141‑1 (MQ=255)
  aaaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATc                                                                                                                                           >  1:104737/1‑148 (MQ=255)
  aaaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATc                                                                                                                                           <  2:2223804/148‑1 (MQ=255)
  aaaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATc                                                                                                                                           >  1:104715/1‑148 (MQ=255)
   aaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATcc                                                                                                                                          <  2:1214694/148‑1 (MQ=255)
   aaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATcc                                                                                                                                          >  2:1430696/1‑148 (MQ=255)
    aaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATccc                                                                                                                                         >  2:408313/1‑148 (MQ=255)
    aaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATccc                                                                                                                                         <  1:3085608/148‑1 (MQ=255)
     aaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATcccc                                                                                                                                        >  2:2070352/1‑148 (MQ=255)
       ccGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCccac                                                                                                                                      >  1:914366/1‑148 (MQ=255)
        cGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCccacc                                                                                                                                     >  1:211312/1‑148 (MQ=255)
          ttttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaa                                                                                                                                   <  1:2247893/148‑1 (MQ=255)
           tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaaa                                                                                                                                  <  2:1766103/148‑1 (MQ=255)
           tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaaa                                                                                                                                  <  2:2989267/148‑1 (MQ=255)
           tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCaaa                                                                                                                                  >  2:3219676/1‑148 (MQ=255)
            ttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAAc                                                                                                                                 <  1:873470/148‑1 (MQ=255)
              gtgCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTg                                                                                                                               <  1:1705903/148‑1 (MQ=255)
                  cATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATa                                                                                                                           <  2:2121708/148‑1 (MQ=255)
                   aTGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATAc                                                                                                                          >  2:44213/1‑148 (MQ=255)
                    tGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGACCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATAcc                                                                                                                         >  2:2049318/1‑148 (MQ=255)
                     gTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCg                                                                                                                        <  2:1552353/148‑1 (MQ=255)
                      tAACTGGCTTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGc                                                                                                                       <  2:1387199/148‑1 (MQ=255)
                      tAACTGGCTTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGc                                                                                                                       <  2:1388157/148‑1 (MQ=255)
                      tAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGc                                                                                                                       <  1:2545654/148‑1 (MQ=255)
                        aCTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTg                                                                                                                     <  1:1434913/148‑1 (MQ=255)
                           ggCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTaa                                                                                                                  >  2:1325110/1‑148 (MQ=255)
                            gCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAAc                                                                                                                 >  2:1037252/1‑148 (MQ=255)
                             cGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAAcc                                                                                                                <  1:2165974/148‑1 (MQ=255)
                              gTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAAccc                                                                                                               <  1:3185397/148‑1 (MQ=255)
                                 cATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCACCACCAAACTGGATCCCGCTGTAACCCCTg                                                                                                            >  2:2858891/1‑148 (MQ=255)
                                   ttCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTc                                                                                                          >  2:1987333/1‑148 (MQ=255)
                                   ttCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTc                                                                                                          >  1:2994434/1‑148 (MQ=255)
                                      ccATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCaaa                                                                                                       <  1:1228833/148‑1 (MQ=255)
                                       cATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAg                                                                                                      <  1:2292138/148‑1 (MQ=255)
                                        aTGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGc                                                                                                     >  1:1866607/1‑148 (MQ=255)
                                          gTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCg                                                                                                   <  1:218225/148‑1 (MQ=255)
                                               cTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAg                                                                                              >  2:1723281/1‑148 (MQ=255)
                                               cTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAg                                                                                              <  1:2758948/148‑1 (MQ=255)
                                                 gAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGtt                                                                                            >  2:2496391/1‑148 (MQ=255)
                                                   aCGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTg                                                                                          >  2:2446643/1‑148 (MQ=255)
                                                    cGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCAGGTCAAAGCCGCTCAGTTAGc                                                                                         >  2:2375212/1‑148 (MQ=255)
                                                      tGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCa                                                                                       >  2:1819723/1‑148 (MQ=255)
                                                       gCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCAc                                                                                      <  1:939308/148‑1 (MQ=255)
                                                           tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  <  2:1597030/148‑1 (MQ=255)
                                                           tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  <  2:1081980/148‑1 (MQ=255)
                                                            aCCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCa                                                                                 >  2:1280249/1‑148 (MQ=255)
                                                            aCCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCa                                                                                 >  2:2600939/1‑148 (MQ=255)
                                                             ccGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCaa                                                                                <  1:1627735/148‑1 (MQ=255)
                                                                tCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAgg                                                                             >  2:1097492/1‑148 (MQ=255)
                                                                 cGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGa                                                                            <  1:73981/148‑1 (MQ=255)
                                                                 cGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGa                                                                            <  1:1748482/148‑1 (MQ=255)
                                                                 cGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGa                                                                            <  1:1430696/148‑1 (MQ=255)
                                                                  gCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAg                                                                           <  2:59554/148‑1 (MQ=255)
                                                                     tCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTcc                                                                        <  2:2425914/148‑1 (MQ=255)
                                                                     tCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTcc                                                                        <  2:1529393/148‑1 (MQ=255)
                                                                     tCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTcc                                                                        <  2:3169679/148‑1 (MQ=255)
                                                                        gCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAgg                                                                     <  1:2898006/148‑1 (MQ=255)
                                                                         cTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGc                                                                    <  1:1947121/148‑1 (MQ=255)
                                                                         cTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGc                                                                    <  2:330904/148‑1 (MQ=255)
                                                                           ataGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCtt                                                                  <  1:3157485/148‑1 (MQ=255)
                                                                            taGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTc                                                                 <  2:976916/148‑1 (MQ=255)
                                                                              gTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTccc                                                               >  1:3276210/1‑148 (MQ=255)
                                                                                ccGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTccccc                                                             <  1:2611004/148‑1 (MQ=255)
                                                                                 cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCa                                                            <  1:1585927/148‑1 (MQ=255)
                                                                                 cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCa                                                            <  1:1585339/148‑1 (MQ=255)
                                                                                   gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  2:1931756/148‑1 (MQ=255)
                                                                                    cGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATaa                                                         >  1:758857/1‑148 (MQ=255)
                                                                                        gCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgc                                                     >  2:2928390/1‑148 (MQ=255)
                                                                                        gCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgc                                                     >  1:2673812/1‑148 (MQ=255)
                                                                                               ttGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCa                                              >  2:782782/1‑148 (MQ=255)
                                                                                                     cAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATAtt                                        <  1:36807/148‑1 (MQ=255)
                                                                                                     cAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATAtt                                        <  1:1472702/148‑1 (MQ=255)
                                                                                                      aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTa                                       <  1:1911259/148‑1 (MQ=255)
                                                                                                      aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTa                                       >  2:722389/1‑148 (MQ=255)
                                                                                                       atatACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTAc                                      >  2:3275215/1‑148 (MQ=255)
                                                                                                         ataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCg                                    >  2:2381877/1‑148 (MQ=255)
                                                                                                         ataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCg                                    >  1:184401/1‑148 (MQ=255)
                                                                                                           aCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTc                                  >  1:3051781/1‑148 (MQ=255)
                                                                                                           aCAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTc                                  <  1:1209319/148‑1 (MQ=255)
                                                                                                           aCAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTc                                  <  1:44213/148‑1 (MQ=255)
                                                                                                                 cGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTAct                            <  2:2597027/148‑1 (MQ=255)
                                                                                                                 cGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTAct                            <  2:104715/148‑1 (MQ=255)
                                                                                                                  gACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActc                           <  1:730464/148‑1 (MQ=255)
                                                                                                                   aCATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct                          >  2:3032374/1‑148 (MQ=255)
                                                                                                                   aCATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct                          >  2:3033099/1‑148 (MQ=255)
                                                                                                                    cATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTg                         <  2:1276210/148‑1 (MQ=255)
                                                                                                                            ggCATTACATCAGAGAGACTTGTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCa                 <  2:1061855/148‑1 (MQ=255)
                                                                                                                              cATTACATCAGAGAGACTTTTATACCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAgg               >  1:1268416/1‑148 (MQ=255)
                                                                                                                               aTTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGt              <  1:1113252/148‑1 (MQ=255)
                                                                                                                               aTTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGt              <  1:1819723/148‑1 (MQ=255)
                                                                                                                               aTTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGt              <  1:2429371/148‑1 (MQ=255)
                                                                                                                                ttACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATCTTACCGTCTTTACTCTGATTTCGCAGGTc             >  2:826695/1‑148 (MQ=255)
                                                                                                                                 tACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCTCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCa            >  1:2021123/1‑148 (MQ=255)
                                                                                                                                 tACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCa            <  1:647316/148‑1 (MQ=255)
                                                                                                                                    aTCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCAccc         >  1:2854868/1‑148 (MQ=255)
                                                                                                                                      cAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCAt       >  2:16921/1‑148 (MQ=255)
                                                                                                                                       agagagACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATc      <  2:922413/148‑1 (MQ=255)
                                                                                                                                           agaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGtt  >  2:1173179/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249084‑1249370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑