BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,251,120	T→A	11.0%	T97S (ACC→TCC)	ycgV ←	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,251,120	0	T	A	11.0%	520.3 / 47.8	182	T97S (ACC→TCC)	ycgV	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (87/73); new base A (9/11); total (98/84)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 183 total aligned reads displayed.

ATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATC  >  CP009273/1250974‑1251267

        aGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCAtt                                                                                                                                            <  2:2915307/148‑1 (MQ=255)

         gTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTa                                                                                                                                           <  1:1420948/148‑1 (MQ=255)

         gTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATACAGAGGCGTCGTTGACATTGACGGTGACATCATTa                                                                                                                                           >  1:3185612/1‑148 (MQ=255)

           cACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAcc                                                                                                                                         >  1:2421538/1‑148 (MQ=255)

            aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAccc                                                                                                                                        <  2:2361331/148‑1 (MQ=255)

              cAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAg                                                                                                                                      >  1:263627/1‑148 (MQ=255)

               aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAgg                                                                                                                                     >  2:2763142/1‑148 (MQ=255)

               aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGGGGTTGAAATTGACGGTGACAAAATTAACCAgg                                                                                                                                     >  1:3068861/1‑148 (MQ=255)

                aTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGt                                                                                                                                    >  2:1651717/1‑148 (MQ=255)

                      ttAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGatat                                                                                                                              >  2:3157207/1‑148 (MQ=255)

                       tAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATAtt                                                                                                                             <  2:683542/148‑1 (MQ=255)

                        aaTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCACTACCCAGGTTGATATTg                                                                                                                            <  2:1698112/148‑1 (MQ=255)

                         aTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTTCATATCCAGAGGCGTCGTTGAAATTTACGGTGACAACATTAACCACGGTGTTAtttg                                                                                                                           >  1:1412593/1‑146 (MQ=255)

                         aTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGc                                                                                                                           <  1:2270606/148‑1 (MQ=255)

                           gCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCa                                                                                                                         >  2:2872058/1‑148 (MQ=255)

                                  gCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCgtgt                                                                                                                  <  2:477771/148‑1 (MQ=255)

                                  gCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCgtgt                                                                                                                  <  2:478839/148‑1 (MQ=255)

                                    agagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTTCCCAGAATAATGATTGCTTTTGCATATCCAGAGGCGTCGTTTTCAATTAAGGTGACAAAATTAACCACGGGTATATTTGCAGGCGTGgtt                                                                                                                >  1:2781337/1‑148 (MQ=255)

                                    agagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGttt                                                                                                                <  2:804920/148‑1 (MQ=255)

                                    agagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGttt                                                                                                                <  2:805098/148‑1 (MQ=255)

                                     gagGTTTGACCAACAACTTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTc                                                                                                               <  2:1033010/148‑1 (MQ=255)

                                     gagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTc                                                                                                               <  1:1052217/148‑1 (MQ=255)

                                     gagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTc                                                                                                               <  2:1032975/148‑1 (MQ=255)

                                      agGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTTACGGTGACATCATTACCCACGTTGATATTGCCAGGCGGGTTTcc                                                                                                              >  1:646231/1‑148 (MQ=255)

                                      agGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTcc                                                                                                              <  2:2250102/148‑1 (MQ=255)

                                      agGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTcc                                                                                                              >  2:1865039/1‑148 (MQ=255)

                                        gTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctg                                                                                                            <  1:1541614/148‑1 (MQ=255)

                                         tttGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGTCATTGACGGGGACATAAATACACAGGTTGATATTTCCAGGCGGGTTTCctgc                                                                                                           >  1:1427002/1‑148 (MQ=255)

                                         tttGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATACAGAGGCGTCGTTGTCATTTAAGGTGACATCATTAACCAGGTTGATATTTCCAGGCGTGTTTActgc                                                                                                           >  1:29380/1‑148 (MQ=255)

                                         tttGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGACCTGAATAATGATTACTTTTGCATATACAGAGGCGTCGTTGACATTTAAGGTGACATCAATACACAAGTTGATATTTCCACGCGTGtttacagc                                                                                                           >  1:1706015/1‑143 (MQ=255)

                                          ttGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgct                                                                                                          >  2:124076/1‑148 (MQ=255)

                                           tGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgctg                                                                                                         <  2:2075532/148‑1 (MQ=255)

                                             aCCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTggg                                                                                                       <  2:2086947/148‑1 (MQ=255)

                                                aacaacATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTc                                                                                                    >  2:2977279/1‑148 (MQ=255)

                                                  caacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAt                                                                                                  >  1:1582944/1‑148 (MQ=255)

                                                  caacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAt                                                                                                  >  2:256909/1‑148 (MQ=255)

                                                     caTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTac                                                                                               >  2:1934591/1‑148 (MQ=255)

                                                     caTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACAATGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTac                                                                                               >  1:1697658/1‑148 (MQ=255)

                                                      aTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTaca                                                                                              <  1:2457012/148‑1 (MQ=255)

                                                       tCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTacac                                                                                             >  1:522794/1‑148 (MQ=255)

                                                          aCTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCAt                                                                                          <  2:414998/148‑1 (MQ=255)

                                                             gtgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATaga                                                                                       <  2:850520/148‑1 (MQ=255)

                                                              tgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTTACGGTGACATAATTAACCAGGTTGATATTTCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                      >  1:768830/1‑148 (MQ=255)

                                                              tgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                      <  2:2046516/148‑1 (MQ=255)

                                                              tgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTACTTTTGCATATCCAGAGGCGTCGTTGACAATGACGGTGACAAAATTACCCAGGTTGATATTGCCAGGCCTGTTTCATGCTGGGGTCAATAAACCAAagat                                                                                      >  1:378404/1‑147 (MQ=255)

                                                               gAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACAATTAAGGTGACATAATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAgg                                                                                     >  1:1240871/1‑148 (MQ=255)

                                                                aGTCGGTTAGCTGTCAGGGAGCTGTTTTTTCCCTGAAAAATGAATCCTTTTTCATAAACAGAGGCGTCGTTGACAATTAAGGTGACAACAATAACCAGGTTGATATTGCCAGGCGTTTTTCCTGCTGGGGTCATTAAACCATAGAGGt                                                                                    >  1:1330514/1‑148 (MQ=255)

                                                                aGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGt                                                                                    <  1:3152250/148‑1 (MQ=255)

                                                                aGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCAGAATAATGATTACTTTTGCATATCCACAGGCGTCGTTGACAATTAACGTGACATCAATACCCACGTTGATATTGCCAGGCGTGTTTACTGCTGGGGTCATTAAACCAAAGAGGt                                                                                    >  1:84169/1‑148 (MQ=255)

                                                                 gTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGAGTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGtt                                                                                   <  2:3302433/148‑1 (MQ=255)

                                                                  tCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGttt                                                                                  >  2:2510771/1‑148 (MQ=255)

                                                                  tCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGttt                                                                                  <  1:633702/148‑1 (MQ=255)

                                                                  tCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATAGTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGttt                                                                                  <  2:3276736/148‑1 (MQ=255)

                                                                  tCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGAAGGAGACATCATTAACCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTAAACCAAATAGGttt                                                                                  >  1:638354/1‑148 (MQ=255)

                                                                      ttAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTg                                                                              >  2:1549111/1‑148 (MQ=255)

                                                                      ttAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTg                                                                              >  1:184434/1‑148 (MQ=255)

                                                                           tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGa                                                                         <  1:3170163/148‑1 (MQ=255)

                                                                           tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGa                                                                         <  1:250465/148‑1 (MQ=255)

                                                                                  gAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCCTATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTAATGCGGGGGTCATTACACCCTAGAGGTTTCATGATGGATcggcgg                                                                  >  2:3044941/1‑148 (MQ=255)

                                                                                   aGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGt                                                                 <  1:2114805/148‑1 (MQ=255)

                                                                                       gTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAtct                                                             >  2:2413281/1‑148 (MQ=255)

                                                                                        tttttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAtctc                                                            <  2:858992/148‑1 (MQ=255)

                                                                                           ttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTg                                                         <  1:1068167/148‑1 (MQ=255)

                                                                                             gCCTTGAATAATGATTCCTTTTGCAGATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTa                                                       <  2:1430631/148‑1 (MQ=255)

                                                                                             gCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTa                                                       <  2:1716244/148‑1 (MQ=255)

                                                                                              cccTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTaa                                                      >  2:1757886/1‑148 (MQ=255)

                                                                                                cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAAtt                                                    <  2:3077569/148‑1 (MQ=255)

                                                                                                cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAAtt                                                    <  2:107229/148‑1 (MQ=255)

                                                                                                 tGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTc                                                   <  1:3293224/148‑1 (MQ=255)

                                                                                                  gAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                  >  1:1593267/1‑148 (MQ=255)

                                                                                                  gAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACAGTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                  >  1:687476/1‑148 (MQ=255)

                                                                                                   aataatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTg                                                 <  1:3157207/148‑1 (MQ=255)

                                                                                                    ataatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGt                                                >  1:655006/1‑148 (MQ=255)

                                                                                                     taatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTTACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTc                                               >  1:968031/1‑148 (MQ=255)

                                                                                                     taatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTc                                               >  1:2681108/1‑148 (MQ=255)

                                                                                                      aatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCa                                              >  2:1816007/1‑148 (MQ=255)

                                                                                                       atGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTTATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCAc                                             >  1:1179613/1‑148 (MQ=255)

                                                                                                        tGAGTCCTTGTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATGACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCAcc                                            <  2:2362067/148‑1 (MQ=255)

                                                                                                           ttCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatc                                         >  1:2607349/1‑148 (MQ=255)

                                                                                                            tCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatca                                        <  1:1898320/148‑1 (MQ=255)

                                                                                                               ttttGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTa                                     <  2:184434/148‑1 (MQ=255)

                                                                                                                       atCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAat                             <  2:321412/148‑1 (MQ=255)

                                                                                                                       atCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAat                             <  2:322034/148‑1 (MQ=255)

                                                                                                                         ccAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAatat                           >  2:35589/1‑148 (MQ=255)

                                                                                                                           agagGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTg                         >  2:8631/1‑148 (MQ=255)

                                                                                                                               gCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGc                     >  1:2391278/1‑148 (MQ=255)

                                                                                                                                cgtcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGTTTTCCTGATGGATAGGCGTTATCTACTGTAATTCTGTCACCATCATTAATAGTAATATTGGttcg                    <  2:1970614/148‑4 (MQ=255)

                                                                                                                                cgtcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGcc                    <  2:794511/148‑1 (MQ=255)

                                                                                                                                cgtcgtGGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGcc                    >  1:486562/1‑148 (MQ=255)

                                                                                                                                  tcgtTGACATTGACGGTGACATCATTACCCAGGTTGATCTTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCat                  >  2:1471435/1‑148 (MQ=255)

                                                                                                                                  tcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCat                  <  1:273596/148‑1 (MQ=255)

                                                                                                                                   cgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCata                 >  1:1915810/1‑148 (MQ=255)

                                                                                                                                     ttGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATAtt               >  2:565237/1‑148 (MQ=255)

                                                                                                                                      tGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTg              <  2:517208/148‑1 (MQ=255)

                                                                                                                                      tGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTg              <  2:517270/148‑1 (MQ=255)

                                                                                                                                      tGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTg              >  1:322791/1‑148 (MQ=255)

                                                                                                                                         cATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACt           >  2:2084570/1‑148 (MQ=255)

                                                                                                                                           ttGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCAGCTGGGGTCATTACACCATAGAGGTTTCCTGATGTATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTc         >  2:145243/1‑148 (MQ=255)

                                                                                                                                            tGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc        >  2:230611/1‑148 (MQ=255)

                                                                                                                                            tGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc        <  2:1412593/148‑1 (MQ=255)

ATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATC  >  CP009273/1250974‑1251267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`