Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,277,786 G→A 100% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,277,7860GA99.5% 611.3 / ‑4.4 183D823N (GAC→AAC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (111/71);  total (112/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGA  >  CP009273/1277644‑1277930
                                                                                                                                              |                                                                                                                                                
tCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTgg                                                                                                                                             >  1:1317210/1‑148 (MQ=255)
   gTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGACGCGAAAAGCAACTGGGaa                                                                                                                                          >  2:64395/1‑148 (MQ=255)
   gTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaa                                                                                                                                          <  1:1888228/148‑1 (MQ=255)
    tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaaa                                                                                                                                         <  1:2799029/148‑1 (MQ=255)
    tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaaa                                                                                                                                         <  1:1653199/148‑1 (MQ=255)
    tCGAGCACCTGTCTATATTCCGAAATCTTTTTGCCGACGGCGACCTGTTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaaa                                                                                                                                         <  1:3064538/148‑1 (MQ=25)
        gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                     >  1:1029868/1‑148 (MQ=255)
         cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                    >  2:2158324/1‑148 (MQ=255)
         cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                    >  1:2838464/1‑148 (MQ=255)
         cACCTGACTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                    >  2:1354325/1‑148 (MQ=255)
          aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACa                                                                                                                                   >  1:2867976/1‑148 (MQ=255)
          aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGCCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACa                                                                                                                                   >  1:2867983/1‑148 (MQ=255)
           ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                  >  2:924423/1‑148 (MQ=255)
            cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaaa                                                                                                                                 >  1:2730697/1‑148 (MQ=255)
                 tctATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAt                                                                                                                            <  2:1114883/148‑1 (MQ=255)
                 tctATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAt                                                                                                                            <  1:1815715/148‑1 (MQ=255)
                 tctATTCCGACATCATTGTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAt                                                                                                                            <  1:1835377/148‑1 (MQ=255)
                     ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATcgcg                                                                                                                        >  2:3229498/1‑148 (MQ=255)
                      tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCga                                                                                                                       >  1:1577665/1‑148 (MQ=255)
                         gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaaga                                                                                                                    <  1:2536149/148‑1 (MQ=255)
                          aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaagaa                                                                                                                   >  2:3113826/1‑148 (MQ=255)
                           catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGaaa                                                                                                                  >  2:172718/1‑148 (MQ=255)
                            atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAt                                                                                                                 >  2:3042293/1‑148 (MQ=255)
                            atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAt                                                                                                                 >  2:3042292/1‑148 (MQ=255)
                            atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAt                                                                                                                 <  1:2629085/148‑1 (MQ=255)
                             tcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAtt                                                                                                                <  1:1236587/148‑1 (MQ=255)
                              catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                               >  1:921149/1‑148 (MQ=255)
                              catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                               >  1:115230/1‑148 (MQ=255)
                               atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                              >  1:510275/1‑148 (MQ=255)
                               atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                              <  2:3194748/148‑1 (MQ=255)
                                ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtctc                                                                                                             <  2:198310/148‑1 (MQ=255)
                                ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtctc                                                                                                             >  1:439880/1‑148 (MQ=255)
                                   tGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa                                                                                                          <  1:1441295/148‑1 (MQ=255)
                                      cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgt                                                                                                       <  1:796203/148‑1 (MQ=255)
                                      cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgt                                                                                                       <  1:796040/148‑1 (MQ=255)
                                        aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                     >  1:2683960/1‑148 (MQ=255)
                                          ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc                                                                                                   <  2:1147425/148‑1 (MQ=255)
                                            cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCACCGCGAAGAAATTCTCCGAAGTGTGCGt                                                                                                 <  1:407187/148‑1 (MQ=255)
                                                cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGc                                                                                             >  2:1189770/1‑148 (MQ=255)
                                                         aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt                                                                                    >  1:1983721/1‑148 (MQ=255)
                                                           aaGACGAAATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGTGAAATCTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                  >  1:3186524/1‑148 (MQ=255)
                                                            aGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                                                 >  2:3086273/1‑148 (MQ=255)
                                                                   aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                                          >  1:1483113/1‑148 (MQ=255)
                                                                    tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGa                                                                         >  2:523018/1‑148 (MQ=255)
                                                                     gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                        <  2:3302833/148‑1 (MQ=255)
                                                                     gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                        <  1:1091951/148‑1 (MQ=255)
                                                                     gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                        <  2:1577665/148‑1 (MQ=255)
                                                                      aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa                                                                       >  2:1285648/1‑148 (MQ=255)
                                                                      aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa                                                                       >  2:2936018/1‑148 (MQ=255)
                                                                      aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa                                                                       <  1:1381564/148‑1 (MQ=255)
                                                                       aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                      <  1:2956395/148‑1 (MQ=255)
                                                                       aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                      <  1:200112/148‑1 (MQ=255)
                                                                        tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                                                     >  1:2501132/1‑148 (MQ=255)
                                                                          cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt                                                                   >  2:1072212/1‑148 (MQ=255)
                                                                           ttCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGta                                                                  <  2:3275878/148‑2 (MQ=255)
                                                                             cGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc                                                                >  1:2225588/1‑148 (MQ=255)
                                                                              ggATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                               >  1:1270442/1‑148 (MQ=255)
                                                                              ggATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                               <  1:128474/148‑1 (MQ=255)
                                                                               gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                                              >  1:2961554/1‑148 (MQ=255)
                                                                               gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                                              >  1:270052/1‑148 (MQ=255)
                                                                               gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                                              >  1:268825/1‑148 (MQ=255)
                                                                                atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCt                                                             >  1:1860739/1‑148 (MQ=255)
                                                                                  atGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                           >  1:2953490/1‑148 (MQ=255)
                                                                                     cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                        >  1:745125/1‑148 (MQ=255)
                                                                                       tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                                                      <  2:1385581/148‑1 (MQ=255)
                                                                                          gTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg                                                   <  2:1849018/148‑1 (MQ=255)
                                                                                           tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGc                                                  >  2:882659/1‑148 (MQ=255)
                                                                                            ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                 >  2:1981100/1‑148 (MQ=255)
                                                                                            ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                 >  1:2515457/1‑148 (MQ=255)
                                                                                            ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                 >  1:743226/1‑148 (MQ=255)
                                                                                               ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa                                              >  1:457831/1‑148 (MQ=255)
                                                                                                tCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAc                                             >  2:3284512/1‑148 (MQ=255)
                                                                                                 cACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAct                                            <  1:1361022/148‑1 (MQ=255)
                                                                                                  aCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc                                           <  1:763899/148‑1 (MQ=255)
                                                                                                   cccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                                          >  2:2628473/1‑148 (MQ=255)
                                                                                                   cccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                                          >  2:2628551/1‑148 (MQ=255)
                                                                                                     cGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGc                                        >  2:1030958/1‑148 (MQ=255)
                                                                                                      gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                       >  2:1830005/1‑148 (MQ=255)
                                                                                                       cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCg                                      >  2:1236589/1‑148 (MQ=255)
                                                                                                       cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCg                                      <  1:1734222/148‑1 (MQ=255)
                                                                                                         gTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt                                    >  2:3282964/1‑148 (MQ=255)
                                                                                                           cTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGa                                  >  1:1277531/1‑148 (MQ=255)
                                                                                                            tGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa                                 <  2:2881620/148‑1 (MQ=255)
                                                                                                                gcggTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg                             >  1:404327/1‑148 (MQ=255)
                                                                                                                    tCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCa                         >  1:2611685/1‑148 (MQ=255)
                                                                                                                    tCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCa                         >  1:957270/1‑148 (MQ=255)
                                                                                                                        tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                     <  1:1469538/148‑1 (MQ=255)
                                                                                                                        tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                     <  1:3113826/148‑1 (MQ=255)
                                                                                                                        tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                     <  2:2867983/148‑1 (MQ=255)
                                                                                                                         ccGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGc                    >  2:1418139/1‑148 (MQ=255)
                                                                                                                         ccGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGc                    >  2:605006/1‑148 (MQ=255)
                                                                                                                            gCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTgg                 <  2:2863869/148‑1 (MQ=255)
                                                                                                                                 ggAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgtg            >  2:893536/1‑148 (MQ=255)
                                                                                                                                  gAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGa           <  2:1727864/148‑1 (MQ=255)
                                                                                                                                  gAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGa           >  2:1455405/1‑148 (MQ=255)
                                                                                                                                   aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGaa          >  2:1002677/1‑148 (MQ=255)
                                                                                                                                   aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGaa          <  2:837401/148‑1 (MQ=255)
                                                                                                                                      cGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGa       <  1:2273221/148‑1 (MQ=255)
                                                                                                                                        aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACt     <  2:754614/148‑1 (MQ=255)
                                                                                                                                           aGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCTACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGa  >  1:672173/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGA  >  CP009273/1277644‑1277930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑