Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 118,873 G→T 100% D99Y (GAC→TAC)  pdhR → pyruvate dehydrogenase complex repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273118,8730GT100.0% 667.4 / NA 202D99Y (GAC→TAC) pdhRpyruvate dehydrogenase complex repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (93/109);  total (93/109)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

TTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAT  >  CP009273/118729‑119017
                                                                                                                                                |                                                                                                                                                
ttGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                               <  1:3111411/148‑1 (MQ=255)
ttGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                               <  1:1772607/148‑1 (MQ=255)
ttGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACt                                                                                                                                               <  1:1470312/148‑1 (MQ=255)
 tGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACtt                                                                                                                                              >  1:669331/1‑148 (MQ=255)
  gCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTg                                                                                                                                             <  1:1839942/148‑1 (MQ=255)
      gAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTCTGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCg                                                                                                                                         >  2:224873/1‑148 (MQ=255)
         gCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGaaa                                                                                                                                      <  2:3036087/148‑1 (MQ=255)
         gCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGaaa                                                                                                                                      >  1:2504488/1‑148 (MQ=255)
         gCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGaaa                                                                                                                                      <  2:318094/148‑1 (MQ=255)
          cGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAac                                                                                                                                     <  2:555495/148‑1 (MQ=255)
           gATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAaca                                                                                                                                    <  1:3268245/148‑1 (MQ=255)
           gATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAaca                                                                                                                                    <  1:2424467/148‑1 (MQ=255)
            aTTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacac                                                                                                                                   >  2:827523/1‑148 (MQ=255)
                aaCGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCATCCTATGGCAAAGCTTAAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgaca                                                                                                                               <  2:3089065/148‑1 (MQ=255)
                 aCGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgacac                                                                                                                              >  1:1440522/1‑148 (MQ=255)
                  cGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgacacg                                                                                                                             >  1:1740368/1‑148 (MQ=255)
                  cGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAacacgacacg                                                                                                                             >  1:2871867/1‑148 (MQ=255)
                   gTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGc                                                                                                                            <  2:2310555/148‑1 (MQ=255)
                    tctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGcc                                                                                                                           <  1:221290/148‑1 (MQ=255)
                     ctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                          <  2:3064204/148‑1 (MQ=255)
                     ctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                          <  2:3063978/148‑1 (MQ=255)
                     ctcGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGccc                                                                                                                          <  2:418088/148‑1 (MQ=255)
                       cgaagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTg                                                                                                                        >  1:1015697/1‑148 (MQ=255)
                        gaagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTgg                                                                                                                       <  2:720516/148‑1 (MQ=255)
                         aagcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGa                                                                                                                      <  2:669331/148‑1 (MQ=255)
                          agcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGaa                                                                                                                     <  1:3068810/148‑1 (MQ=255)
                           gcgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAg                                                                                                                    <  1:2822360/148‑1 (MQ=255)
                           gcgaagGGCGTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAg                                                                                                                    >  2:2443585/1‑148 (MQ=255)
                            cgaagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAgg                                                                                                                   >  2:2796911/1‑148 (MQ=255)
                              aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTa                                                                                                                 <  1:10955/148‑1 (MQ=255)
                              aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTa                                                                                                                 <  1:349039/148‑1 (MQ=255)
                              aagGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTa                                                                                                                 <  1:2337954/148‑1 (MQ=255)
                               agGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                <  1:536163/148‑1 (MQ=255)
                               agGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                <  2:2024816/148‑1 (MQ=255)
                               agGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                <  1:915037/148‑1 (MQ=255)
                               agGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTAt                                                                                                                <  1:28416/148‑1 (MQ=255)
                                gggCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATc                                                                                                               >  2:958468/1‑148 (MQ=255)
                                    ttgttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATcgccg                                                                                                           <  1:332836/148‑1 (MQ=255)
                                     tgttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATcgccgc                                                                                                          <  1:621416/148‑1 (MQ=255)
                                     ggttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATcgccgc                                                                                                          <  1:2785167/147‑1 (MQ=255)
                                       ttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtt                                                                                                        <  2:2504488/148‑1 (MQ=255)
                                       ttgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtt                                                                                                        >  2:4794/1‑148 (MQ=255)
                                        tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtta                                                                                                       >  2:1543574/1‑148 (MQ=255)
                                        tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtta                                                                                                       <  2:2532783/148‑1 (MQ=255)
                                        tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtta                                                                                                       <  2:85801/148‑1 (MQ=255)
                                        tgCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCtta                                                                                                       <  1:1162196/148‑1 (MQ=255)
                                          cTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatt                                                                                                     >  2:1037149/1‑148 (MQ=255)
                                          cTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatt                                                                                                     >  2:2918949/1‑148 (MQ=255)
                                           ttCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCttatta                                                                                                    <  1:1040214/148‑1 (MQ=255)
                                            tcgtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAc                                                                                                   >  1:2667228/1‑148 (MQ=255)
                                            tcgtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGACTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCTAAACACGACACTCCCTGGAAGGTATCGCCGCTTATTAc                                                                                                   >  1:2640658/1‑148 (MQ=255)
                                             cgtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcg                                                                                                  <  2:1856535/148‑1 (MQ=255)
                                             cgtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcg                                                                                                  <  2:1561394/148‑1 (MQ=255)
                                              gtcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcgc                                                                                                 >  2:2994535/1‑148 (MQ=255)
                                               tcgCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAcgcc                                                                                                >  1:2321430/1‑148 (MQ=255)
                                                  ccAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCcgcg                                                                                             <  2:2002655/148‑1 (MQ=255)
                                                    aGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCt                                                                                           >  2:249788/1‑148 (MQ=255)
                                                     gggTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTg                                                                                          <  2:156775/148‑1 (MQ=255)
                                                      ggTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGc                                                                                         >  2:2234791/1‑148 (MQ=255)
                                                         ggcggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgta                                                                                      <  1:471538/148‑1 (MQ=255)
                                                           cggcACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCgtagt                                                                                    <  2:380712/148‑1 (MQ=255)
                                                              cACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCGTATTACGCCGCGCTGCGTAGTAcc                                                                                 >  1:884529/1‑148 (MQ=255)
                                                               aCTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCg                                                                                <  1:1211459/148‑1 (MQ=255)
                                                                cTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGa                                                                               >  2:173086/1‑148 (MQ=255)
                                                                  tttGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATg                                                                             >  1:183349/1‑148 (MQ=255)
                                                                       ccAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGAc                                                                        <  1:270601/148‑1 (MQ=255)
                                                                        cAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACa                                                                       <  1:3186104/148‑1 (MQ=255)
                                                                            gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGa                                                                   <  2:1700300/148‑1 (MQ=255)
                                                                               gcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACg                                                                >  1:1251831/1‑148 (MQ=255)
                                                                               gcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACg                                                                <  2:3046185/148‑1 (MQ=255)
                                                                               gcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACg                                                                <  2:2699002/148‑1 (MQ=255)
                                                                                ccTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGc                                                               >  2:447171/1‑148 (MQ=255)
                                                                                  tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                             <  1:773254/148‑1 (MQ=255)
                                                                                  tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAAGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCAt                                                             >  1:3058719/1‑148 (MQ=255)
                                                                                     ggCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCGTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCg                                                          >  1:599411/1‑148 (MQ=255)
                                                                                      gCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGt                                                         >  2:1593532/1‑148 (MQ=255)
                                                                                       cAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTg                                                        >  2:3120127/1‑148 (MQ=255)
                                                                                       cAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTg                                                        >  2:396104/1‑148 (MQ=255)
                                                                                       cAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTg                                                        <  1:1530045/148‑1 (MQ=255)
                                                                                        aaaGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGa                                                       >  1:1619061/1‑148 (MQ=255)
                                                                                                  cGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCcaccac                                             >  2:1457229/1‑148 (MQ=255)
                                                                                                         cTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATag                                      <  1:3012927/148‑1 (MQ=255)
                                                                                                         cTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATag                                      <  2:3070867/148‑1 (MQ=255)
                                                                                                            gtggAGCTGCTCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGc                                   >  2:2591873/1‑148 (MQ=255)
                                                                                                                 gctgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTAAGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgcg                              >  1:1454150/1‑148 (MQ=255)
                                                                                                                  ctgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgcgc                             <  2:2691907/148‑1 (MQ=255)
                                                                                                                  ctgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGgcgc                             >  2:1596512/1‑148 (MQ=255)
                                                                                                                  ctgctCTCCGACCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTGATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATACAGCTGgcgc                             >  2:2345694/1‑148 (MQ=255)
                                                                                                                            aCCATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTgg                   <  1:3163699/148‑1 (MQ=255)
                                                                                                                             ccATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGc                  <  1:1037164/148‑1 (MQ=255)
                                                                                                                             ccATCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGc                  >  2:1996088/1‑148 (MQ=255)
                                                                                                                                tCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGAt               >  2:1289623/1‑148 (MQ=255)
                                                                                                                                tCCTGAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGAt               <  1:756735/148‑1 (MQ=255)
                                                                                                                                   tGAGTCACAGTATTACTTGCACGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTAACGATTAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCCAtatt            >  2:1062959/1‑145 (MQ=255)
                                                                                                                                    gAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTgg           >  2:1405823/1‑148 (MQ=255)
                                                                                                                                    gAGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATACGTGAAATACAACACGCCATAGAGCTGGCGCAGCAGTCTTGCGATCTgg           >  2:1406193/1‑148 (MQ=255)
                                                                                                                                     aGTCACAGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGa          <  1:424724/148‑1 (MQ=255)
                                                                                                                                        cacaGTATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGAcgc       >  1:2949947/1‑148 (MQ=255)
                                                                                                                                             tATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt  >  2:36505/1‑148 (MQ=255)
                                                                                                                                             tATTACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAt  <  2:2463080/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGCGATCTGGACGCGGAAT  >  CP009273/118729‑119017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑