BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,330,955	G→T	93.4%	D290Y (GAT→TAT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,330,955	0	G	T	93.4%	541.8 / 30.9	196	D290Y (GAT→TAT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base G (5/8); new base T (93/90); total (98/98)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 202 total aligned reads displayed.

CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG  >  CP009273/1330808‑1331099

ccGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                                                                                                  >  1:2201148/1‑148 (MQ=255)

  ggTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAt                                                                                                                                                <  1:2370284/148‑1 (MQ=255)

  ggTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAt                                                                                                                                                <  2:2379684/148‑1 (MQ=255)

      tCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTc                                                                                                                                            >  1:2187998/1‑148 (MQ=255)

         aTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTgg                                                                                                                                         >  1:2616659/1‑148 (MQ=255)

          tGCTTATCCCGGCTGTAGTTGGATTCAAACTTACCGGAATATTACGTGAAGGTATTACCGCCACAGACCTGGGTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGa                                                                                                                                        <  1:346030/148‑1 (MQ=255)

          tGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGa                                                                                                                                        <  2:2212468/148‑1 (MQ=255)

            cTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAtt                                                                                                                                      <  1:1706430/148‑1 (MQ=255)

               aTCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCAc                                                                                                                                   <  1:191229/148‑1 (MQ=255)

                tCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACt                                                                                                                                  <  1:1329900/148‑1 (MQ=255)

                tCCCGGATGTAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACt                                                                                                                                  >  2:3196849/1‑148 (MQ=255)

                  ccGGAGGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACAGGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTAc                                                                                                                                >  2:1634208/1‑148 (MQ=255)

                     gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGt                                                                                                                             >  2:835479/1‑148 (MQ=255)

                     gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGt                                                                                                                             >  2:3001190/1‑148 (MQ=255)

                     gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGt                                                                                                                             <  2:875221/148‑1 (MQ=255)

                      aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGtt                                                                                                                            <  2:2499477/148‑1 (MQ=255)

                       tGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTg                                                                                                                           <  2:44415/148‑1 (MQ=255)

                         tAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGc                                                                                                                         >  1:2042500/1‑148 (MQ=255)

                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      >  1:2691376/1‑148 (MQ=255)

                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      <  2:748016/148‑1 (MQ=255)

                             gggCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGAt                                                                                                                     <  1:2856630/148‑1 (MQ=255)

                              ggCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATc                                                                                                                    >  1:3130692/1‑148 (MQ=255)

                               gCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATcg                                                                                                                   <  1:3187120/148‑1 (MQ=255)

                                  tCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGCAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                                                                                                >  1:1369129/1‑148 (MQ=255)

                                    aaaCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGcca                                                                                                              <  1:2348016/148‑1 (MQ=255)

                                     aaCTTACCGGAAAATTACGTGAAGGTATTACCGCCATAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccac                                                                                                             >  2:1855458/1‑148 (MQ=255)

                                      aCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                            >  2:2809769/1‑148 (MQ=255)

                                      aCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                            >  2:3280024/1‑148 (MQ=255)

                                       ctgaCCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           <  2:1858775/145‑1 (MQ=255)

                                       cTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           >  1:762475/1‑148 (MQ=255)

                                             ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCa                                                                                                     <  2:2608154/148‑1 (MQ=255)

                                               aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                   >  1:2088885/1‑148 (MQ=255)

                                                aaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAata                                                                                                  >  2:1814883/1‑148 (MQ=255)

                                                  aTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATg                                                                                                >  1:938316/1‑148 (MQ=255)

                                                   ttACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGt                                                                                               >  2:310273/1‑148 (MQ=255)

                                                     aCGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCg                                                                                             >  1:1711525/1‑148 (MQ=255)

                                                       gTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGcc                                                                                           >  2:753657/1‑148 (MQ=255)

                                                       gTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGcc                                                                                           <  1:1879492/148‑1 (MQ=255)

                                                        tGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCa                                                                                          >  2:2826211/1‑148 (MQ=255)

                                                          aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGa                                                                                        <  1:1417413/148‑1 (MQ=255)

                                                          aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGa                                                                                        <  2:38850/148‑1 (MQ=255)

                                                           aGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGAGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGaa                                                                                       <  1:2916560/148‑1 (MQ=255)

                                                            ggTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAat                                                                                      <  2:724533/148‑1 (MQ=255)

                                                            ggTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAat                                                                                      >  1:2777904/1‑148 (MQ=255)

                                                             gTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAata                                                                                     >  1:3118959/1‑148 (MQ=255)

                                                              tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                    <  1:3208542/148‑1 (MQ=255)

                                                              tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                    <  1:997067/148‑1 (MQ=255)

                                                              tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                    >  1:1453579/1‑148 (MQ=255)

                                                               aTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGTATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg                                                                                   <  1:1880647/148‑1 (MQ=255)

                                                               aTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg                                                                                   >  1:2451918/1‑148 (MQ=255)

                                                                  aCCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                <  2:805929/148‑1 (MQ=255)

                                                                   ccGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGc                                                                               <  2:2011868/148‑1 (MQ=255)

                                                                   ccGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGc                                                                               >  1:159702/1‑148 (MQ=255)

                                                                      ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAc                                                                            <  1:1064932/148‑1 (MQ=255)

                                                                       cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAcc                                                                           >  1:1849921/1‑148 (MQ=255)

                                                                        acaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCt                                                                          >  2:1774955/1‑148 (MQ=255)

                                                                          aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtgt                                                                        >  1:1142146/1‑148 (MQ=255)

                                                                           gACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtgtg                                                                       >  1:384992/1‑148 (MQ=255)

                                                                             ccTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGc                                                                     <  1:2041152/148‑1 (MQ=255)

                                                                                ggTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                  <  1:2404744/148‑1 (MQ=255)

                                                                                 gTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttct                                                                 >  2:3087610/1‑148 (MQ=255)

                                                                                  ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                >  2:2729251/1‑148 (MQ=255)

                                                                                  ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                >  1:2804250/1‑148 (MQ=255)

                                                                                   tctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCttcttc                                                               <  1:83204/148‑1 (MQ=255)

                                                                                   tctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCttcttc                                                               <  1:851037/148‑1 (MQ=255)

                                                                                     tcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTacc                                                             >  1:3068827/1‑148 (MQ=255)

                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                  <  2:639425/142‑1 (MQ=255)

                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                  >  1:639425/1‑142 (MQ=255)

                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                            <  2:2187998/148‑1 (MQ=255)

                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                            >  2:113976/1‑148 (MQ=255)

                                                                                        cTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAt                                                          <  2:311273/148‑1 (MQ=255)

                                                                                            tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                      >  2:1414520/1‑148 (MQ=255)

                                                                                              cccAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGc                                                    >  1:530001/1‑148 (MQ=255)

                                                                                                  aaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                >  1:89632/1‑148 (MQ=255)

                                                                                                         cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCg                                         <  1:1480581/148‑1 (MQ=255)

                                                                                                               cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                   <  2:1552969/148‑1 (MQ=255)

                                                                                                               cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                   >  2:1291672/1‑148 (MQ=255)

                                                                                                               cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                   <  2:1861457/148‑1 (MQ=255)

                                                                                                               cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                   <  2:3130692/148‑1 (MQ=255)

                                                                                                                aTGGCGTGGCGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACAt                                  >  2:666236/1‑148 (MQ=255)

                                                                                                                 tGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATg                                 <  2:3143256/148‑1 (MQ=255)

                                                                                                                    cGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt                              <  2:2794709/148‑1 (MQ=255)

                                                                                                                    cGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt                              <  2:1142146/148‑1 (MQ=255)

                                                                                                                       ggtggGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTa                           <  1:1981300/148‑1 (MQ=255)

                                                                                                                            ggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCgg                      >  2:1760287/1‑148 (MQ=255)

                                                                                                                             gAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCggg                     <  2:206381/148‑1 (MQ=255)

                                                                                                                                 ttCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCa                 >  1:727878/1‑148 (MQ=255)

                                                                                                                                     tcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa             >  2:3121098/1‑148 (MQ=255)

                                                                                                                                      cgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGaa            >  2:62568/1‑148 (MQ=255)

                                                                                                                                        aaTTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCCGCGAAGa          >  2:2875454/1‑148 (MQ=255)

                                                                                                                                        aaTTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa          >  1:392791/1‑148 (MQ=255)

                                                                                                                                        aaTTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa          <  2:2201148/148‑1 (MQ=255)

                                                                                                                                         aTTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGAt         >  2:1494206/1‑148 (MQ=255)

                                                                                                                                          ttttATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc        <  2:1465022/148‑1 (MQ=255)

                                                                                                                                           tttATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCa       >  2:1628282/1‑148 (MQ=255)

                                                                                                                                            ttatggttatggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAg      <  2:1984461/148‑1 (MQ=255)

                                                                                                                                             tatggttatggtCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAgg     <  2:1894084/148‑1 (MQ=255)

                                                                                                                                             tatggttatggtCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAgg     <  2:2687114/148‑1 (MQ=255)

                                                                                                                                              aTGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGt    <  2:2423645/148‑1 (MQ=255)

                                                                                                                                                ggTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg  <  1:2559438/148‑1 (MQ=255)

CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG  >  CP009273/1330808‑1331099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`