| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,365,283 | G→T | 100% | E271* (GAG→TAG) | ycjM → | alpha amylase catalytic domain family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,365,283 | 0 | G | T | 99.3% | 558.6 / ‑5.7 | 190 | E271* (GAG→TAG) | ycjM | alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (93/91); minor base A (5/0); total (99/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 192 total aligned reads displayed.
GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG > CP009273/1365139‑1365426 | gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga < 2:2722740/148‑1 (MQ=255) gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa < 1:2451731/148‑1 (MQ=255) gTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt < 2:937952/148‑1 (MQ=255) tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg < 1:2750604/148‑1 (MQ=255) tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt < 2:205633/148‑1 (MQ=255) cTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa < 1:2807653/148‑1 (MQ=255) cTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa < 1:2807639/148‑1 (MQ=255) tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa < 1:1886705/148‑1 (MQ=255) tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa < 1:2729614/148‑1 (MQ=255) gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTTTTGATAAAGTTGCGCCACGTACAGTGATCATTAACAATAACAAAGTTACGCATAAAg > 1:2222816/1‑148 (MQ=255) gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACTTTTCGCCACGTACAGTTATAATTAACTAGAACAAAGTTACGCAAAAAg > 1:561189/1‑148 (MQ=255) gCCGTTGGCTTTATGTGGAGAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑a < 2:3286614/148‑1 (MQ=255) gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑a < 1:265819/148‑1 (MQ=255) cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑aac < 1:1033449/148‑1 (MQ=255) ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt > 2:2667706/1‑148 (MQ=255) ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt < 2:2745854/148‑1 (MQ=255) ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt > 2:2489937/1‑148 (MQ=255) gCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGc > 2:1353227/1‑148 (MQ=255) cTTTATGTGGAAAGAGCCGGGAACAAGCTGAATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTAATTATAAAGTTTCCCCAGGTACAGTGATCAATACCTAGACCAATTTTACGCATAAATAA‑AAAATTTCt > 2:1691119/1‑148 (MQ=255) tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCtt < 2:3171498/148‑1 (MQ=255) ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTa < 1:2650133/148‑1 (MQ=255) ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTa < 1:2650804/148‑1 (MQ=255) tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTAc < 1:2285602/148‑1 (MQ=255) aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGGACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTGACTTTGgcg > 1:910642/1‑148 (MQ=255) agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAgg < 2:127127/148‑1 (MQ=255) agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAgg < 2:126172/148‑1 (MQ=255) ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCg > 2:834534/1‑148 (MQ=255) cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGCTCATTACCTAGACCAATTTTCCGCATAAATAC‑AACATTGCTTAATTTGGCGCAGGCGa > 1:2980780/1‑148 (MQ=255) cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGa < 2:2994515/148‑1 (MQ=255) aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg > 2:3180249/1‑148 (MQ=255) aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg > 1:2726096/1‑148 (MQ=255) aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg < 2:2001532/148‑1 (MQ=255) cTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGca < 1:2592348/148‑1 (MQ=255) gCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGcaca < 1:3166428/148‑1 (MQ=255) atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata < 1:2124054/148‑1 (MQ=255) atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata < 1:2124131/148‑1 (MQ=255) atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata < 2:1454857/148‑1 (MQ=255) ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg < 2:1627520/148‑1 (MQ=255) tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTACGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATtgtg > 2:1021350/1‑148 (MQ=255) cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATATCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt < 2:406372/148‑1 (MQ=255) cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt < 1:1407682/148‑1 (MQ=255) ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACGTTGGCGCCGGCGATGACGAAGCACAGATGGTGTAc > 2:2811329/1‑148 (MQ=255) gAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc > 1:3025398/1‑148 (MQ=255) aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTACCAg > 2:507234/1‑148 (MQ=255) aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCACGTACAGTGATCATTACCTATACCAAAGTTCCCCAAAAATAC‑AACAATTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt > 1:2921795/1‑148 (MQ=255) aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt < 1:906469/148‑1 (MQ=255) aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGAAAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCCGt > 2:1475729/1‑148 (MQ=255) aaaaCACATCTGAGTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt < 2:1999329/148‑1 (MQ=255) aaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt < 1:355728/148‑1 (MQ=255) aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc < 2:1849747/148‑1 (MQ=255) acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTTCCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct > 2:2581641/1‑148 (MQ=255) acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct < 2:2837166/148‑1 (MQ=255) cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGGTCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACCTATGGTGTACCAGTtctc > 2:3084137/1‑148 (MQ=255) cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc > 2:1548977/1‑148 (MQ=255) cacaTCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTAAACTGATCATTAACAAGAACAATGTTCCCCATAAAGACAAACATT‑CTTACTTTTGCCCAGGCGATTACGAAGCACATATGGTGTACCAGttata > 1:677271/1‑145 (MQ=255) caTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGc < 2:1744359/148‑1 (MQ=255) tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg < 2:401253/148‑1 (MQ=255) tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg < 2:1285255/148‑1 (MQ=255) tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGAC‑AACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg > 2:1496464/1‑148 (MQ=255) tATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATTGTGTACCAGTTCTCGCTGCCGCCa > 1:527870/1‑148 (MQ=255) tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATGATTACCTAGACCAATGTTCCGCATAAAGAA‑AACAATGCTTACTTTGGCGCAGGCGCTGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa > 1:2404661/1‑148 (MQ=255) tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa > 2:1086377/1‑148 (MQ=255) cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAA‑AACATTTCTTAATTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTg > 1:2678201/1‑148 (MQ=255) aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGTCGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg > 2:1218597/1‑148 (MQ=255) aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg < 1:1967298/148‑1 (MQ=255) tGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct > 2:2438882/1‑148 (MQ=255) cGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATg < 2:2846606/148‑1 (MQ=255) tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg > 2:1818204/1‑148 (MQ=255) tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg > 2:2666365/1‑148 (MQ=255) attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaa > 2:678050/1‑148 (MQ=255) aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa > 2:170235/1‑148 (MQ=255) aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa > 2:2113784/1‑148 (MQ=255) tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg > 2:230297/1‑148 (MQ=255) tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg > 2:2606280/1‑148 (MQ=255) tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg > 1:1059568/1‑148 (MQ=255) aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa > 2:463898/1‑148 (MQ=255) gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg < 2:561189/148‑1 (MQ=255) gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg < 1:62635/148‑1 (MQ=255) gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg < 2:2222816/148‑1 (MQ=255) cgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa < 1:3013230/148‑1 (MQ=255) aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg < 2:264033/148‑1 (MQ=255) ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc > 2:2793006/1‑148 (MQ=255) ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc > 2:1640071/1‑148 (MQ=255) gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt > 2:2067134/1‑148 (MQ=255) gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt < 1:1310237/148‑1 (MQ=255) gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCACAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt < 1:2489942/148‑1 (MQ=255) tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt < 2:2981725/148‑1 (MQ=255) tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt < 1:2941450/148‑1 (MQ=255) aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt > 2:2295261/1‑148 (MQ=255) gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg > 1:101592/1‑148 (MQ=255) gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg < 1:2112949/148‑1 (MQ=255) gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg > 2:2197800/1‑148 (MQ=255) gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg > 2:2101527/1‑148 (MQ=255) aTCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGt > 2:733953/1‑148 (MQ=255) cATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg > 1:287153/1‑148 (MQ=255) cATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg > 1:3061664/1‑148 (MQ=255) aTTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTggg > 1:1124035/1‑148 (MQ=255) tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg > 2:3124102/1‑148 (MQ=255) tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg > 2:711715/1‑148 (MQ=255) tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGNACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg > 1:1677893/1‑148 (MQ=255) | GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG > CP009273/1365139‑1365426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |