Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,424,234 C→A 100% S310* (TCA→TAA)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,424,2340CA98.9% 575.1 / ‑1.5 177S310* (TCA→TAA) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (89/86);  total (91/86)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 181 total aligned reads displayed.

TCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCA  >  CP009273/1424089‑1424376
                                                                                                                                                 |                                                                                                                                              
tCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaa                                                                                                                                              <  1:523160/148‑1 (MQ=255)
tCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGAAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaa                                                                                                                                              <  1:2459436/148‑1 (MQ=255)
  cTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTaaaca                                                                                                                                            >  1:265393/1‑148 (MQ=255)
    cGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAg                                                                                                                                          <  1:3039634/148‑1 (MQ=255)
    cGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAg                                                                                                                                          <  1:1018655/148‑1 (MQ=255)
    cGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAg                                                                                                                                          <  2:1615807/148‑1 (MQ=255)
      gCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTg                                                                                                                                        >  2:2430185/1‑148 (MQ=255)
       cAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGc                                                                                                                                       >  1:3146441/1‑148 (MQ=255)
          cggcggCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCgg                                                                                                                                    >  2:1199549/1‑148 (MQ=255)
          cggcggCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCgg                                                                                                                                    >  2:2850501/1‑148 (MQ=255)
                cAGGAAATTCCGCGAAGGCGACAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGc                                                                                                                              >  1:2041873/1‑148 (MQ=255)
                  ggAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCt                                                                                                                            >  2:1167688/1‑148 (MQ=255)
                   taaaTTCCGCGAGGGCGGAAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTc                                                                                                                           <  1:1490714/147‑1 (MQ=255)
                   gAAATTCCGCGACGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTc                                                                                                                           <  1:3282604/148‑1 (MQ=255)
                   gAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTc                                                                                                                           <  2:2263663/148‑1 (MQ=255)
                   gAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTc                                                                                                                           >  1:2080895/1‑148 (MQ=255)
                   gAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTc                                                                                                                           >  1:219128/1‑148 (MQ=255)
                        tCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCa                                                                                                                      >  2:3185093/1‑148 (MQ=255)
                            cgAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGc                                                                                                                  >  2:798623/1‑148 (MQ=255)
                              aaGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCa                                                                                                                >  2:351220/1‑148 (MQ=255)
                              aaGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCa                                                                                                                <  1:3293216/148‑1 (MQ=255)
                                 gcggcAAAACCGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccg                                                                                                             <  2:2016227/148‑1 (MQ=255)
                                 gcggcAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccg                                                                                                             >  1:1171681/1‑148 (MQ=255)
                                     cAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCAccgccgc                                                                                                         >  2:2213458/1‑148 (MQ=255)
                                           cGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGaaa                                                                                                   <  1:2281632/148‑1 (MQ=255)
                                            gTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGaaaa                                                                                                  <  2:2274496/148‑1 (MQ=255)
                                             tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAAt                                                                                                 >  1:2338484/1‑148 (MQ=255)
                                             tCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAAt                                                                                                 >  1:888785/1‑148 (MQ=255)
                                               cGAGACGACCGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCg                                                                                               <  2:909141/148‑1 (MQ=255)
                                                gagaCGAACGCCTGTTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCgg                                                                                              <  2:2513214/148‑1 (MQ=255)
                                                gagaCGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCgg                                                                                              <  2:2513347/148‑1 (MQ=255)
                                                  gaCGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCa                                                                                            >  2:1250700/1‑148 (MQ=255)
                                                         gCCTGGTCTTCTGAAACGGCAGCGGGAGAGAGCGCCTCGGCTGCGGCAGGCTCAAACACCGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAgcg                                                                                     <  2:2885609/148‑1 (MQ=255)
                                                         gCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAgcg                                                                                     <  1:350911/148‑1 (MQ=255)
                                                          ccAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAgcgc                                                                                    <  2:2442610/148‑1 (MQ=255)
                                                             ggTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGcgccgc                                                                                 >  2:2782142/1‑148 (MQ=255)
                                                              gTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCa                                                                                >  1:2297278/1‑148 (MQ=255)
                                                              gTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCa                                                                                >  2:3097301/1‑148 (MQ=255)
                                                               tcttctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCAt                                                                               <  2:2576705/148‑1 (MQ=255)
                                                                  tctGAAACGGCAGCGGGACAGAGCGCCTGGGCTGCGGCTGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                            <  2:3063442/148‑1 (MQ=255)
                                                                  tctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                            <  2:145962/148‑1 (MQ=255)
                                                                  tctGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGt                                                                            >  1:463602/1‑148 (MQ=255)
                                                                    tGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCt                                                                          <  2:627255/148‑1 (MQ=255)
                                                                        aCGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACCGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCtt                                                                      <  2:301208/148‑1 (MQ=255)
                                                                        aCGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCtt                                                                      >  2:1821574/1‑148 (MQ=255)
                                                                          ggCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCa                                                                    <  2:641666/148‑1 (MQ=255)
                                                                              gCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAg                                                                >  2:471731/1‑148 (MQ=255)
                                                                              gCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAg                                                                <  1:3202752/148‑1 (MQ=255)
                                                                               cGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGc                                                               <  1:275261/148‑1 (MQ=255)
                                                                                gggACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGcc                                                              <  1:73678/148‑1 (MQ=255)
                                                                                   aCAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCTGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcaca                                                           <  1:2069830/148‑1 (MQ=255)
                                                                                   aCAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCcaca                                                           <  1:2069824/148‑1 (MQ=255)
                                                                                      gagCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACg                                                        >  2:911377/1‑148 (MQ=255)
                                                                                       agCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGa                                                       >  2:121452/1‑148 (MQ=255)
                                                                                       agCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCAAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGa                                                       >  2:924171/1‑148 (MQ=255)
                                                                                        gcgcCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGaa                                                      <  2:1457186/148‑1 (MQ=255)
                                                                                        gcgcCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGaa                                                      >  1:2989981/1‑148 (MQ=255)
                                                                                          gcCTCGGCTGCGGCAGGCTCAAAACCAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAgg                                                    <  1:1964390/148‑1 (MQ=255)
                                                                                           ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                   >  2:1764443/1‑148 (MQ=255)
                                                                                           ccTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGc                                                   <  2:776505/148‑1 (MQ=255)
                                                                                            cTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCt                                                  >  1:175657/1‑148 (MQ=255)
                                                                                             tCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTg                                                 >  1:385947/1‑148 (MQ=255)
                                                                                             tCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTg                                                 >  1:1611546/1‑148 (MQ=255)
                                                                                                 cTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGa                                             >  1:159993/1‑148 (MQ=255)
                                                                                                   gCGGCAGGATCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAg                                           <  1:2549980/148‑1 (MQ=255)
                                                                                                    cGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGc                                          <  2:1853031/148‑1 (MQ=255)
                                                                                                    cGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGc                                          <  1:3112249/148‑1 (MQ=255)
                                                                                                      gCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCTTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                        <  1:339117/148‑1 (MQ=255)
                                                                                                      gCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCa                                        <  2:2570942/148‑1 (MQ=255)
                                                                                                       cAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCAc                                       <  1:1278261/148‑1 (MQ=255)
                                                                                                         ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTg                                     >  2:411208/1‑148 (MQ=255)
                                                                                                         ggCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTg                                     >  2:3070002/1‑148 (MQ=255)
                                                                                                           cTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                   >  1:2274761/1‑148 (MQ=255)
                                                                                                           cTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGaa                                   <  2:2666309/148‑1 (MQ=255)
                                                                                                            tCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACACGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAAc                                  <  1:1463319/148‑1 (MQ=255)
                                                                                                            acAAAAACCGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGGGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAAc                                  <  1:1463307/147‑1 (MQ=255)
                                                                                                                   cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAg                           >  1:2430408/1‑148 (MQ=255)
                                                                                                                   cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAg                           <  2:516906/148‑1 (MQ=255)
                                                                                                                   cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAg                           >  1:3092204/1‑148 (MQ=255)
                                                                                                                   cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAg                           >  2:2682651/1‑148 (MQ=255)
                                                                                                                   cAGCGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAg                           <  2:1701828/148‑1 (MQ=255)
                                                                                                                      cGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAgcgc                        >  2:2415172/1‑148 (MQ=255)
                                                                                                                      cGGCTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAgcgc                        >  2:2725967/1‑148 (MQ=255)
                                                                                                                         cTGCGTCGTCTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCgcagc                     <  1:2890343/148‑1 (MQ=255)
                                                                                                                              tcgtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGa                >  2:1340391/1‑148 (MQ=255)
                                                                                                                              tcgtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGa                >  1:633817/1‑148 (MQ=255)
                                                                                                                               cgtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAg               >  1:938939/1‑148 (MQ=255)
                                                                                                                                gtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAgg              <  1:2508317/148‑1 (MQ=255)
                                                                                                                                gtcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAgg              <  1:1090738/148‑1 (MQ=255)
                                                                                                                                 tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGt             <  2:3146441/148‑1 (MQ=255)
                                                                                                                                 tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGt             <  2:2157215/148‑1 (MQ=255)
                                                                                                                                 tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGt             <  2:1986682/148‑1 (MQ=255)
                                                                                                                                 tcTGCCAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGt             <  1:2817795/148‑1 (MQ=255)
                                                                                                                                     ccAGTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGc         <  2:1328848/148‑1 (MQ=255)
                                                                                                                                        gTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc      >  1:1584597/1‑148 (MQ=255)
                                                                                                                                        gTGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTc      >  1:1584588/1‑148 (MQ=255)
                                                                                                                                         tGCAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTcc     >  1:126373/1‑148 (MQ=255)
                                                                                                                                           cAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGc   >  1:412709/1‑148 (MQ=255)
                                                                                                                                           cAGCGTAAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGc   <  1:2430185/148‑1 (MQ=255)
                                                                                                                                           cAGCGTAAACAAGTGCCGGGCAGGCCTCAGCATGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGATCAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGc   <  1:2874293/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAGCGTCAACAAGTGCCGGGCAGGCCTCAGCCAGTGCCACCGCCGCCGGAAAATCGGCAGAAAGCGCCGCATCGTCTGCTTCAACAGCCACAACGAAGGCTGGCGAAGCCACTGAACAGGCCAGCGCAGCAGCGAGGTCTGCTTCCGCA  >  CP009273/1424089‑1424376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑