Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,478,971 G→A 100% E552K (GAG→AAG)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,478,9710GA91.7% 703.1 / 43.6 234E552K (GAG→AAG) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base A (104/109);  total (113/121)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 235 total aligned reads displayed.

CGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC  >  CP009273/1478827‑1479115
                                                                                                                                                |                                                                                                                                                
cgTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGt                                                                                                                                               <  1:3044664/148‑1 (MQ=255)
   aTGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTg                                                                                                                                            >  1:2390831/1‑148 (MQ=255)
    tGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGa                                                                                                                                           <  2:101448/148‑1 (MQ=255)
     ggTGCTGGACGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGAc                                                                                                                                          >  2:2351078/1‑148 (MQ=255)
     ggTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGAc                                                                                                                                          >  2:584027/1‑148 (MQ=255)
     ggTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGAc                                                                                                                                          <  2:369202/148‑1 (MQ=255)
        gCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACttt                                                                                                                                       <  2:3192851/148‑1 (MQ=255)
            gAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcg                                                                                                                                   >  1:982861/1‑148 (MQ=255)
             aaGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcgc                                                                                                                                  >  1:1611020/1‑148 (MQ=255)
             aaGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcgc                                                                                                                                  >  1:1611329/1‑148 (MQ=255)
             aaGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcgc                                                                                                                                  >  2:3096213/1‑148 (MQ=255)
              aGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTcgcg                                                                                                                                 >  2:2888019/1‑148 (MQ=255)
                 gcAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGttt                                                                                                                              <  1:2269091/148‑1 (MQ=255)
                     aaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                          >  2:3196861/1‑148 (MQ=255)
                     aaaCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGa                                                                                                                          <  1:1510846/148‑1 (MQ=255)
                       aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAt                                                                                                                        <  2:452375/148‑1 (MQ=255)
                       aCATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAAt                                                                                                                        >  2:2021197/1‑148 (MQ=255)
                        cATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATc                                                                                                                       >  2:2754679/1‑148 (MQ=255)
                          tGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCtg                                                                                                                     <  2:767004/148‑1 (MQ=255)
                          tGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCtg                                                                                                                     <  2:1929606/148‑1 (MQ=255)
                           ggCTGAGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGTACAAACAGCAGGCATCGGACGAAAACCATCGTCGCTTCGACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCtgt                                                                                                                    <  2:1382854/148‑1 (MQ=255)
                               gcgtgcgtGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGaa                                                                                                                <  2:1537891/148‑1 (MQ=255)
                                  tgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCTGACTAAAAACATCGTCGATTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTa                                                                                                             <  1:3165450/148‑1 (MQ=255)
                                  tgcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTa                                                                                                             <  1:1009691/148‑1 (MQ=255)
                                   gcgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTAt                                                                                                            >  1:2661062/1‑148 (MQ=255)
                                    cgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATc                                                                                                           <  1:477387/148‑1 (MQ=255)
                                    cgtgAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATc                                                                                                           <  1:477340/148‑1 (MQ=255)
                                           cGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGa                                                                                                    >  2:79069/1‑148 (MQ=255)
                                                 ttATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAgcagca                                                                                              <  1:92048/148‑1 (MQ=255)
                                                   aTCACGTCCGCGCTCTCCATTCCGGATGCGAGAGAACGTCCGAGGGACAAACAGCAGTCATCGGACGAAAAACATCGTCGCTTCCTCGACTAAAAGTCTGACTTTCTCGCTTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaaa                                                                                            <  2:1718525/148‑1 (MQ=255)
                                                   aTCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaaa                                                                                            <  1:731674/148‑1 (MQ=255)
                                                   aTCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGACTTATCTTGGCGAGCAGCaaa                                                                                            >  2:2166017/1‑148 (MQ=255)
                                                    tCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCaaaa                                                                                           >  2:1123319/1‑148 (MQ=255)
                                                     cACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAg                                                                                          >  2:805772/1‑148 (MQ=255)
                                                     cACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAg                                                                                          >  2:2080366/1‑148 (MQ=255)
                                                      aCGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAgg                                                                                         <  1:2907388/148‑1 (MQ=255)
                                                       cGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGgc                                                                                        >  1:972558/1‑148 (MQ=255)
                                                        gTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGATCAGCAAAAGgcg                                                                                       >  1:1120789/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  2:1876813/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  1:2149898/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  1:1199210/1‑148 (MQ=255)
                                                          ccGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCt                                                                                     >  1:2650566/1‑148 (MQ=255)
                                                            gcgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCttt                                                                                   <  2:1363113/148‑1 (MQ=255)
                                                            gcgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCttt                                                                                   <  2:3010595/148‑1 (MQ=255)
                                                             cgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttc                                                                                  <  2:2734228/148‑1 (MQ=255)
                                                             cgcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttc                                                                                  >  2:417422/1‑148 (MQ=255)
                                                              gcTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTttct                                                                                 <  2:2390831/148‑1 (MQ=255)
                                                                 ctcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTcc                                                                              >  1:2852512/1‑148 (MQ=255)
                                                                  tcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCa                                                                             <  2:2915158/148‑1 (MQ=255)
                                                                  tcCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCa                                                                             >  2:38939/1‑148 (MQ=255)
                                                                   ccATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCaa                                                                            >  1:1775238/1‑148 (MQ=255)
                                                                     aTTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACg                                                                          >  1:611342/1‑148 (MQ=255)
                                                                      ttCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGc                                                                         <  2:2486530/148‑1 (MQ=255)
                                                                       tCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGcc                                                                        <  2:2822865/148‑1 (MQ=255)
                                                                       tCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGcc                                                                        <  1:1893614/148‑1 (MQ=255)
                                                                        cAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACAAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCt                                                                       <  1:1318509/148‑1 (MQ=255)
                                                                          ggATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTc                                                                     <  2:2650566/148‑1 (MQ=255)
                                                                          ggATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTc                                                                     >  2:2476249/1‑148 (MQ=255)
                                                                                 cgcgAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGcc                                                              <  1:2577471/148‑1 (MQ=255)
                                                                                     aaCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTATCGCGGTTGTGAATCTGTGGAATTATCTTGGCGAGCAGAAAAAGTCGCTTTATTCCAACGCCATCCGTCGCCtttg                                                          >  1:2362238/1‑148 (MQ=255)
                                                                                     aaCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCtgtg                                                          >  1:567156/1‑148 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         <  2:90910/148‑1 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         >  2:976526/1‑148 (MQ=255)
                                                                                      aCGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTgtgt                                                         >  2:2631421/1‑148 (MQ=255)
                                                                                       cGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTc                                                        >  2:1616787/1‑148 (MQ=255)
                                                                                             aTGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCg                                                  >  1:3215981/1‑148 (MQ=255)
                                                                                             aTGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCg                                                  >  2:2125850/1‑148 (MQ=255)
                                                                                              tGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGa                                                 <  1:2409548/148‑1 (MQ=255)
                                                                                              tGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGa                                                 <  1:2998768/148‑1 (MQ=255)
                                                                                                  cAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTTCGTCGCCTGTGTCGTACCGATTAt                                             <  2:1960728/148‑1 (MQ=255)
                                                                                                     aCAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAtctc                                          >  1:1857182/1‑148 (MQ=255)
                                                                                                     aCAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTAtctc                                          >  1:1484243/1‑148 (MQ=255)
                                                                                                      cagcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCa                                         <  1:2136138/148‑1 (MQ=255)
                                                                                                       agcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCaa                                        <  1:1544210/148‑1 (MQ=255)
                                                                                                       agcagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCaa                                        <  1:3199695/148‑1 (MQ=255)
                                                                                                         cagGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACt                                      >  1:1189363/1‑148 (MQ=255)
                                                                                                          agGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTa                                     <  2:1159027/148‑1 (MQ=255)
                                                                                                            gCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATc                                   <  2:2317268/148‑1 (MQ=255)
                                                                                                            gCATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATc                                   <  2:1445408/148‑1 (MQ=255)
                                                                                                             cATCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCt                                  >  2:3082055/1‑148 (MQ=255)
                                                                                                              aTCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtg                                 <  2:2255077/148‑1 (MQ=255)
                                                                                                              aTCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtg                                 <  2:2063909/148‑1 (MQ=255)
                                                                                                               tCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTTTGAATCTGTGGAATTATATTGGCGAGCCGCAAAAGGCGCTTTCTTCCAACGCCTTCCCTCGCCGGTGTCGTACCGATTATCACAACTATCtga                                >  1:294812/1‑147 (MQ=255)
                                                                                                               tCGGACGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgc                                >  2:1089861/1‑148 (MQ=255)
                                                                                                                   aCGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgt                            >  2:2429100/1‑148 (MQ=255)
                                                                                                                   aCGAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAAGTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTAAAtgcgcgt                            >  1:2201721/1‑148 (MQ=255)
                                                                                                                     gAAAAACATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCtgcgcgtgc                          >  1:2034313/1‑148 (MQ=255)
                                                                                                                         aaCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGa                      <  1:627685/148‑1 (MQ=255)
                                                                                                                          aCATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGaa                     >  1:194308/1‑148 (MQ=255)
                                                                                                                           cATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAt                    <  2:484497/148‑1 (MQ=255)
                                                                                                                           cATCGTCGCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAAt                    <  1:2773720/148‑1 (MQ=255)
                                                                                                                             tcgtcgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATgg                  <  1:1123319/148‑1 (MQ=255)
                                                                                                                              cgtcgCTTCCACGACAACAAGTCTGACTTTCTCGCGGGTGGGAATCGGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCCACGCCTTCCGTCGCCTGTGTCGTACCGCTTCTCTCAACGATCTGGGCGGGCGCGacgggg                 >  2:2713692/1‑143 (MQ=255)
                                                                                                                              cgtcgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGATCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGc                 <  2:1120789/148‑1 (MQ=255)
                                                                                                                              cgtcgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGc                 <  2:1339166/148‑1 (MQ=255)
                                                                                                                                 cgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAgg              <  1:171419/148‑1 (MQ=255)
                                                                                                                                 cgCTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAgg              >  2:3079377/1‑148 (MQ=255)
                                                                                                                                   cTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGat            <  2:2871662/148‑1 (MQ=255)
                                                                                                                                   cTTCCACGACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGat            <  1:3082055/148‑1 (MQ=255)
                                                                                                                                          gACAAAAAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTaca     <  1:1927423/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATTATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCATCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAATCTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTGTGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCC  >  CP009273/1478827‑1479115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑