Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,495,770 T→G 9.5% L314W (TTG→TGG)  tehA → potassium‑tellurite ethidium and proflavin transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,495,7700TG 9.5% 525.4 / 50.6 200L314W (TTG→TGG) tehApotassium‑tellurite ethidium and proflavin transporter
Reads supporting (aligned to +/- strand):  ref base T (76/105);  new base G (4/15);  total (80/120)
Fisher's exact test for biased strand distribution p-value = 8.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 206 total aligned reads displayed.

CTGCACTGGCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCGGGTAAA  >  CP009273/1495623‑1495917
                                                                                                                                                   |                                                                                                                                                   
cTGCACTGGCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAAtt                                                                                                                                                     <  2:799778/148‑1 (MQ=255)
        gCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCa                                                                                                                                             >  2:839097/1‑148 (MQ=255)
          aaCCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGa                                                                                                                                           <  2:2771500/148‑1 (MQ=255)
          aaCCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGa                                                                                                                                           <  2:286665/148‑1 (MQ=255)
          aaCCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGa                                                                                                                                           <  2:810172/148‑1 (MQ=255)
           accaccGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGaa                                                                                                                                          <  1:1324602/148‑1 (MQ=255)
           accaccGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGATGGCGGTGCCGCTGTTTATCTTTACCAAAATTATTATTGCAATACTGCTCATCCGTACTTTTTAGCTTCATATGCAGGGAACAATGTTAGTCAGaa                                                                                                                                          >  1:1497928/1‑148 (MQ=255)
             caccGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAAcc                                                                                                                                        >  1:1086233/1‑148 (MQ=255)
             caccGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAAcc                                                                                                                                        >  2:3274371/1‑148 (MQ=255)
                cGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAg                                                                                                                                     >  1:2173357/1‑148 (MQ=255)
                  gTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAgcg                                                                                                                                   >  2:615531/1‑148 (MQ=255)
                         tCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGtt                                                                                                                            <  1:2888769/148‑1 (MQ=255)
                          cTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGttt                                                                                                                           <  1:2098918/148‑1 (MQ=255)
                          cTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGttt                                                                                                                           >  2:2500604/1‑148 (MQ=255)
                            ggggAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTa                                                                                                                         <  2:1086233/148‑1 (MQ=255)
                            ggggAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTa                                                                                                                         <  2:3123047/148‑1 (MQ=255)
                            ggggAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTa                                                                                                                         <  1:2665001/148‑1 (MQ=255)
                                 gTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGaa                                                                                                                    <  1:1204167/148‑1 (MQ=255)
                                 gTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGaa                                                                                                                    <  2:2203802/148‑1 (MQ=255)
                                   ggCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAg                                                                                                                  <  1:1626728/148‑1 (MQ=255)
                                   ggCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAg                                                                                                                  <  2:1736021/148‑1 (MQ=255)
                                           tAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGAc                                                                                                          >  2:1639541/1‑148 (MQ=255)
                                           tAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGAc                                                                                                          >  2:1211698/1‑148 (MQ=255)
                                           tAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGAc                                                                                                          >  2:1864012/1‑148 (MQ=255)
                                           tAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGAc                                                                                                          <  2:5069/148‑1 (MQ=255)
                                               ggATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCATTACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAg                                                                                                      <  2:1149928/148‑1 (MQ=255)
                                                 aTTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGaa                                                                                                    >  2:3105508/1‑148 (MQ=255)
                                                 aTTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGaa                                                                                                    <  1:2441898/148‑1 (MQ=255)
                                                 aTTTTTCCATACGCTGGCGGTGCCGCTGTTTATCGTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGaa                                                                                                    >  2:1771235/1‑148 (MQ=255)
                                                  tttttCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAAt                                                                                                   >  2:1788979/1‑148 (MQ=255)
                                                    tttCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGa                                                                                                 <  2:3161212/148‑1 (MQ=255)
                                                     ttCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGAt                                                                                                <  1:631883/148‑1 (MQ=255)
                                                      tCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATc                                                                                               <  1:2788235/148‑1 (MQ=255)
                                                      tCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATc                                                                                               <  2:2918992/148‑1 (MQ=255)
                                                        cATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCAt                                                                                             <  1:2897146/148‑1 (MQ=255)
                                                         aTACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTGGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCAtt                                                                                            <  2:1408664/148‑1 (MQ=255)
                                                             gCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTg                                                                                        <  1:199842/148‑1 (MQ=255)
                                                                   ggTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGaaa                                                                                  <  1:1809922/148‑1 (MQ=255)
                                                                      gCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACt                                                                               <  1:1396959/148‑1 (MQ=255)
                                                                        cGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTAt                                                                             <  1:3255274/148‑1 (MQ=255)
                                                                         gCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTAtt                                                                            >  1:3130726/1‑148 (MQ=255)
                                                                          cTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTAttt                                                                           >  2:1908150/1‑148 (MQ=255)
                                                                          cTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTAttt                                                                           <  2:2021043/148‑1 (MQ=255)
                                                                           tGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTAtttt                                                                          >  2:1858704/1‑148 (MQ=255)
                                                                            gTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTa                                                                         <  2:1941241/148‑1 (MQ=255)
                                                                            gTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTa                                                                         >  1:2945870/1‑148 (MQ=255)
                                                                            gTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTa                                                                         >  2:443636/1‑148 (MQ=255)
                                                                             tttATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGATGACAAAGAATGATCATTCGTGACGAAAACTATTTTAc                                                                        >  1:3071612/1‑148 (MQ=255)
                                                                             tttATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTAc                                                                        >  2:855003/1‑148 (MQ=255)
                                                                               tATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTg                                                                      <  2:2917599/148‑1 (MQ=255)
                                                                                 tCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGAt                                                                    >  2:687011/1‑148 (MQ=255)
                                                                                   tttACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATaa                                                                  >  1:1183657/1‑148 (MQ=255)
                                                                                   tttACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATaa                                                                  <  2:1263783/148‑1 (MQ=255)
                                                                                   tttACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATaa                                                                  <  1:839097/148‑1 (MQ=255)
                                                                                     tACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAat                                                                <  1:1783836/148‑1 (MQ=255)
                                                                                     tACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAat                                                                >  2:2682842/1‑148 (MQ=255)
                                                                                        cAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATg                                                             <  2:1683138/148‑1 (MQ=255)
                                                                                        cAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATg                                                             <  2:1402689/148‑1 (MQ=255)
                                                                                        cAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATg                                                             <  2:1402329/148‑1 (MQ=255)
                                                                                         aaTTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGa                                                            <  2:1591848/148‑1 (MQ=255)
                                                                                          aTTTTTTTATTGCAAGACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAAGCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGaa                                                           <  2:2358371/148‑1 (MQ=255)
                                                                                          aTTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGaa                                                           >  2:2072758/1‑148 (MQ=255)
                                                                                           ttttATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAAt                                                          <  2:2616288/148‑1 (MQ=255)
                                                                                            tttATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAAtt                                                         >  1:2580982/1‑148 (MQ=255)
                                                                                              tattagTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTaa                                                       <  2:1034651/148‑1 (MQ=255)
                                                                                               attattGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAAc                                                      <  1:124535/148‑1 (MQ=255)
                                                                                               attattGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAAc                                                      <  1:2035128/148‑1 (MQ=255)
                                                                                                 tattGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAAccc                                                    >  2:216134/1‑148 (MQ=255)
                                                                                                    tGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGca                                                 >  2:2509869/1‑148 (MQ=255)
                                                                                                        aTACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACACGcacaca                                             >  1:3187885/1‑148 (MQ=255)
                                                                                                         tACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCacacac                                            >  1:873071/1‑148 (MQ=255)
                                                                                                         tACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCacacac                                            >  1:2628141/1‑148 (MQ=255)
                                                                                                             gCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTg                                        <  2:617314/148‑1 (MQ=255)
                                                                                                             gCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTg                                        >  2:3093788/1‑148 (MQ=255)
                                                                                                              cTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGa                                       <  1:3030959/148‑1 (MQ=255)
                                                                                                                  tCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTa                                   >  2:2647733/1‑148 (MQ=255)
                                                                                                                   ccGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTAc                                  <  2:2309280/148‑1 (MQ=255)
                                                                                                                    cGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACt                                 <  2:1806931/148‑1 (MQ=255)
                                                                                                                     gTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTg                                >  2:2456738/1‑148 (MQ=255)
                                                                                                                      tACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTgg                               >  1:2716435/1‑148 (MQ=255)
                                                                                                                       aCTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGa                              >  2:1651002/1‑148 (MQ=255)
                                                                                                                       aCTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGa                              <  1:1901305/148‑1 (MQ=255)
                                                                                                                         ttttGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAg                            <  2:2280067/148‑1 (MQ=255)
                                                                                                                           ttGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCg                          <  1:2401820/148‑1 (MQ=255)
                                                                                                                            tGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATAATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCgg                         <  2:262767/148‑1 (MQ=255)
                                                                                                                              cgcTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTg                       >  2:3242432/1‑148 (MQ=255)
                                                                                                                                  tCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAg                   <  2:1747778/148‑1 (MQ=255)
                                                                                                                                    tGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTg                 <  2:784494/148‑1 (MQ=255)
                                                                                                                                     gATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTgg                >  1:3213356/1‑148 (MQ=255)
                                                                                                                                     gATGCAGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTgg                <  1:2743439/148‑1 (MQ=255)
                                                                                                                                      aTGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGt               >  2:2837811/1‑148 (MQ=255)
                                                                                                                                       tGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGtt              <  2:1499886/148‑1 (MQ=255)
                                                                                                                                        gCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTa             <  2:1338424/148‑1 (MQ=255)
                                                                                                                                          aGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTaaa           >  2:1865066/1‑148 (MQ=255)
                                                                                                                                          aGGGAAAATGGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTaaa           <  1:2500604/148‑1 (MQ=255)
                                                                                                                                            ggAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAAcc         >  1:2321642/1‑148 (MQ=255)
                                                                                                                                             gAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCg        <  1:431886/148‑1 (MQ=255)
                                                                                                                                             gAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCg        <  1:3105508/148‑1 (MQ=255)
                                                                                                                                                   tGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCGGGTaaa  >  1:3275814/1‑148 (MQ=255)
                                                                                                                                                   tGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCGGGTaaa  >  1:1309607/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGCACTGGCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAATGGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATACTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGCGCCGTTTTAATGAAAGCAGAGGACAAAGAATGATCATTCGTGACGAAAACTATTTTACTGATAAATATGAATTAACCCGCACACACTCTGAAGTACTGGAAGCGGTGAAAGTGGTTAAACCGGGTAAA  >  CP009273/1495623‑1495917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑