Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,520,123 C→A 26.6% A39S (GCC→TCC)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,520,1230CA26.6% 549.7 / 155.1 248A39S (GCC→TCC) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (91/91);  new base A (24/42);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 6.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

TGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCAT  >  CP009273/1519978‑1520270
                                                                                                                                                 |                                                                                                                                                   
tgtAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGa                                                                                                                                                   >  2:2649033/1‑148 (MQ=255)
  tAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                                                                                                                                 >  2:2689699/1‑148 (MQ=255)
  tAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGatc                                                                                                                                                 <  1:2825977/148‑1 (MQ=255)
    gCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAACTTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGatcat                                                                                                                                               <  1:1245660/148‑1 (MQ=255)
    gCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatcat                                                                                                                                               <  2:1308399/148‑1 (MQ=255)
     cACCAGCTCACCCAATGCACGCAGAAAAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatcatc                                                                                                                                              <  1:675576/148‑1 (MQ=255)
       ccAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                                                                                                                            >  1:2525142/1‑148 (MQ=255)
          gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCAc                                                                                                                                         >  2:276828/1‑148 (MQ=255)
           cTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCAcc                                                                                                                                        <  1:2669403/148‑1 (MQ=255)
            tCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                                                                                                                                       <  2:36915/148‑1 (MQ=255)
               cccAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGc                                                                                                                                    >  1:3050037/1‑148 (MQ=255)
                ccAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCg                                                                                                                                   >  1:1422589/1‑148 (MQ=255)
                  aaTGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGAt                                                                                                                                 <  2:1796128/148‑1 (MQ=255)
                  aaTGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGAt                                                                                                                                 >  2:1125672/1‑148 (MQ=255)
                    tGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTg                                                                                                                               <  1:2923610/148‑1 (MQ=255)
                      cACGCAGAATAAAATACGAAAACACGCCACAACTTAAATAAAGCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGTTTGcc                                                                                                                             <  2:658852/148‑1 (MQ=255)
                      cACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGcc                                                                                                                             <  1:1381108/148‑1 (MQ=255)
                       aCGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGccc                                                                                                                            <  1:3227652/148‑1 (MQ=255)
                         gCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCAt                                                                                                                          <  2:2981894/148‑1 (MQ=255)
                           aGAATAAAAAACGAACACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCg                                                                                                                        <  2:1973397/148‑1 (MQ=255)
                            gAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGc                                                                                                                       >  1:1718632/1‑148 (MQ=255)
                            gAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGc                                                                                                                       >  1:1718448/1‑148 (MQ=255)
                             aaTAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                                                                                      <  1:3272497/148‑1 (MQ=255)
                             aaTAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                                                                                      >  2:2850372/1‑148 (MQ=255)
                             aaTAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCt                                                                                                                      >  1:2270183/1‑148 (MQ=255)
                             aaTAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCt                                                                                                                      <  1:3007935/148‑1 (MQ=255)
                               tAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCttt                                                                                                                    >  2:2444402/1‑148 (MQ=255)
                                aaaaaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACACGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtg                                                                                                                   <  2:2984687/148‑1 (MQ=255)
                                  aaaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtgtg                                                                                                                 >  1:290030/1‑148 (MQ=255)
                                   aaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGa                                                                                                                >  1:2888066/1‑148 (MQ=255)
                                     aCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATa                                                                                                              >  2:1463061/1‑148 (MQ=255)
                                     aCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATa                                                                                                              >  2:884401/1‑148 (MQ=255)
                                         aaaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAcccc                                                                                                          >  2:2123327/1‑148 (MQ=255)
                                         aaaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAcccc                                                                                                          >  2:2729255/1‑148 (MQ=255)
                                          aaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCct                                                                                                         <  1:2233509/148‑1 (MQ=255)
                                           aCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCctc                                                                                                        >  1:2793707/1‑148 (MQ=255)
                                           aCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCctc                                                                                                        <  2:3271709/148‑1 (MQ=255)
                                            cAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGAGATACCCctct                                                                                                       >  2:1942452/1‑148 (MQ=255)
                                              aGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTc                                                                                                     >  1:2663684/1‑148 (MQ=255)
                                               gCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCg                                                                                                    >  1:553637/1‑148 (MQ=255)
                                               gCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCg                                                                                                    >  1:553929/1‑148 (MQ=255)
                                                ccACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTAGt                                                                                                   >  1:810052/1‑148 (MQ=255)
                                                 cacaAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTg                                                                                                  <  1:3124195/148‑1 (MQ=255)
                                                    aaaTTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGc                                                                                               <  2:2577658/148‑1 (MQ=255)
                                                     aaTTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCa                                                                                              <  1:2592586/148‑1 (MQ=255)
                                                        taaataaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTa                                                                                           <  2:1136320/148‑1 (MQ=255)
                                                          aataaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAg                                                                                         >  1:636054/1‑148 (MQ=255)
                                                           ataaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGc                                                                                        >  2:2227767/1‑148 (MQ=255)
                                                           ataaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGc                                                                                        >  2:4067/1‑148 (MQ=255)
                                                             aaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCa                                                                                      >  2:799992/1‑148 (MQ=255)
                                                             aaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCa                                                                                      >  2:2524178/1‑148 (MQ=255)
                                                             aaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCa                                                                                      >  2:1198872/1‑148 (MQ=255)
                                                              aaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAg                                                                                     <  1:2721070/148‑1 (MQ=255)
                                                              aaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAg                                                                                     >  1:1305950/1‑148 (MQ=255)
                                                               aCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgc                                                                                    <  1:2345344/148‑1 (MQ=255)
                                                               aCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgc                                                                                    >  2:2478285/1‑148 (MQ=255)
                                                                   gtgccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgcggcg                                                                                >  2:1067075/1‑148 (MQ=255)
                                                                    tgccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGt                                                                               <  1:1686437/148‑1 (MQ=255)
                                                                              ggCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCgcggcgg                                                                     >  2:2798189/1‑147 (MQ=255)
                                                                              ggCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCgt                                                                     >  2:1736990/1‑148 (MQ=255)
                                                                                  ccGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTg                                                                 <  2:2242835/148‑1 (MQ=255)
                                                                                   cGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGa                                                                <  1:2937351/148‑1 (MQ=255)
                                                                                        tttGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGa                                                           >  2:1456026/1‑148 (MQ=255)
                                                                                         ttGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGaa                                                          >  1:441009/1‑148 (MQ=255)
                                                                                          tGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAg                                                         <  1:1884603/148‑1 (MQ=255)
                                                                                             aGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAgtgt                                                      <  2:3060664/148‑1 (MQ=255)
                                                                                              gTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAgtgtc                                                     <  1:2131059/148‑1 (MQ=255)
                                                                                               tCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAgtgtcg                                                    >  1:274185/1‑148 (MQ=255)
                                                                                                      ccTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCgt                                             >  2:1809934/1‑148 (MQ=255)
                                                                                                       cTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCgtg                                            >  1:1672890/1‑148 (MQ=255)
                                                                                                         gctcattaaaaCACGCCGGTGCGTATCTCGCCGCCTATTGCGATCTTCTGCACCTTGCGATTGCCCATCGCTTTGTGATAACCCTCTTCGTGGGCATTAAGCCAGCGGAGTTTCGCGGCGTGTTGATGTGAAGTGTCGGTGTCGTGtt                                          <  1:1942452/141‑1 (MQ=255)
                                                                                                         gCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGtt                                          <  1:270493/148‑1 (MQ=255)
                                                                                                           aCCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTa                                        <  2:2108941/148‑1 (MQ=255)
                                                                                                           aCCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTa                                        <  1:1780091/148‑1 (MQ=255)
                                                                                                            ccTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTAc                                       >  2:3144209/1‑148 (MQ=255)
                                                                                                              tAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTc                                     <  1:146642/148‑1 (MQ=255)
                                                                                                               aaaaaCAAGCCGGTGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCa                                    <  1:75520/148‑1 (MQ=255)
                                                                                                               aaaaaCAAGCCGGGGCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCa                                    <  1:1750353/148‑1 (MQ=255)
                                                                                                                   aCAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGc                                >  2:432997/1‑148 (MQ=255)
                                                                                                                       gCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCtctc                            >  2:1669670/1‑148 (MQ=255)
                                                                                                                        ccGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTcc                           <  1:3300747/148‑1 (MQ=255)
                                                                                                                           gTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTg                        <  2:553550/148‑1 (MQ=255)
                                                                                                                             gacAATCGCGCCGCAATTGGCGATGATCTGCTCCTGGCTATTGCCCATCTGTTTGTGATACCCCTCTTCGTGTGGATTAAGCCTGCGGCGTTTCGCGGGGTGTTGTTGTGAAGTGTCGGTGTCTTGTTTTCTCTTTGCTCTCCATGAt                      <  2:761085/146‑1 (MQ=255)
                                                                                                                             gCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGAt                      <  2:714055/148‑1 (MQ=255)
                                                                                                                             gCCAATCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGAt                      <  2:1053390/148‑1 (MQ=255)
                                                                                                                                aaTCGCGCCGCCAATGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGc                   <  1:11045/148‑1 (MQ=255)
                                                                                                                                 aTCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCt                  >  2:86934/1‑148 (MQ=255)
                                                                                                                                  tCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCtt                 <  1:2690847/148‑1 (MQ=255)
                                                                                                                                  tCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCtt                 <  1:1142487/148‑1 (MQ=255)
                                                                                                                                  tCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGGGTCGTGGTTACTCATTGCTCTCCCTGATTGCtt                 >  2:614558/1‑148 (MQ=255)
                                                                                                                                   cgcgCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCttt                >  2:1162796/1‑148 (MQ=255)
                                                                                                                                          ccAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaa         <  1:1034079/148‑1 (MQ=255)
                                                                                                                                          ccAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaa         <  1:643409/148‑1 (MQ=255)
                                                                                                                                          ccAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaa         <  1:1033773/148‑1 (MQ=255)
                                                                                                                                           cAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaaa        <  2:2674396/148‑1 (MQ=255)
                                                                                                                                            aaTGGCGATCATCTGCACCTGGCTATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaaaa       <  1:3178760/148‑1 (MQ=255)
                                                                                                                                            aaTGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaaaa       <  2:2382169/148‑1 (MQ=255)
                                                                                                                                             aTGGAGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAg      <  2:1272803/148‑1 (MQ=255)
                                                                                                                                               ggCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTc    <  1:813076/148‑1 (MQ=255)
                                                                                                                                                 cGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCat  <  1:1799857/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCAT  >  CP009273/1519978‑1520270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑