Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,526,607 G→A 100% D179N (GAT→AAT)  ydcC → H repeat‑associated putative transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,526,6070GA99.2% 301.3 / ‑4.2 129D179N (GAT→AAT) ydcCH repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (70/58);  total (71/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 131 total aligned reads displayed.

CTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTG  >  CP009273/1526518‑1526751
                                                                                         |                                                                                                                                                
cTACTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                        <  2:3047305/148‑1 (MQ=11)
cTAATGAGATTACATCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                        <  2:3047302/148‑1 (MQ=11)
cTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                        >  2:29982/1‑148 (MQ=11)
cTAATGAGATTACAGCTATCCCAGAACGTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                        >  2:35419/1‑148 (MQ=11)
 tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAc                                                                                       <  2:1607333/148‑1 (MQ=14)
 tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAc                                                                                       <  2:1606080/148‑1 (MQ=14)
  aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAcc                                                                                      <  2:2498846/148‑1 (MQ=17)
  aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAcc                                                                                      >  1:3296781/1‑148 (MQ=17)
   aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCa                                                                                     >  1:1005901/1‑148 (MQ=17)
   aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCa                                                                                     <  1:2254830/148‑1 (MQ=17)
    tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAg                                                                                    <  1:1948301/148‑1 (MQ=17)
    tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAg                                                                                    <  1:1549988/148‑1 (MQ=17)
     gagaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgg                                                                                   >  1:1600038/1‑148 (MQ=17)
     gagaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgg                                                                                   >  2:3773/1‑148 (MQ=17)
     gagaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgg                                                                                   >  1:3072583/1‑148 (MQ=17)
       gaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgggg                                                                                 >  1:2113991/1‑148 (MQ=17)
       gaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgggg                                                                                 >  2:2530836/1‑148 (MQ=17)
        aTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggc                                                                                >  1:1916077/1‑148 (MQ=17)
        aTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggc                                                                                >  1:131885/1‑148 (MQ=17)
         ttACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggcg                                                                               <  2:3302540/148‑1 (MQ=17)
          tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggcgg                                                                              >  1:1440754/1‑148 (MQ=17)
          tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggcgg                                                                              >  2:2282841/1‑148 (MQ=17)
          tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggcgg                                                                              <  1:467895/148‑1 (MQ=17)
               cTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                         <  2:559643/148‑1 (MQ=17)
               cTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                         <  2:559651/148‑1 (MQ=17)
               cTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                         >  2:2398491/1‑148 (MQ=17)
                 aTCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaata                                                                       >  2:1336581/1‑148 (MQ=17)
                 aTCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaata                                                                       <  1:240961/148‑1 (MQ=17)
                  tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataa                                                                      >  2:3039631/1‑148 (MQ=17)
                  tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataa                                                                      >  1:3255618/1‑148 (MQ=17)
                  tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataa                                                                      <  1:2438605/148‑1 (MQ=17)
                    ccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAg                                                                    >  1:2446141/1‑148 (MQ=17)
                     cAGAACGTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGc                                                                   <  1:714931/148‑1 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                <  1:2456275/148‑1 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                <  1:2456302/148‑1 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                <  1:2396556/148‑1 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                >  1:2350443/1‑148 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                >  1:1297913/1‑148 (MQ=17)
                        aaCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                >  1:1278565/1‑148 (MQ=17)
                         aCTTCTTAACATGCTGTATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACTGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCttt                                                               <  1:1299462/148‑1 (MQ=12)
                         aCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCttt                                                               >  2:955241/1‑148 (MQ=17)
                           ttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGa                                                             <  2:2751452/148‑1 (MQ=17)
                             cttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAgg                                                           >  1:794732/1‑148 (MQ=17)
                               tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGaa                                                         >  1:1737587/1‑148 (MQ=17)
                                  cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaaa                                                      <  2:2217385/148‑1 (MQ=17)
                                  cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaaa                                                      >  1:743241/1‑148 (MQ=17)
                                    tGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt                                                    <  1:2380754/148‑1 (MQ=17)
                                     gCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAttt                                                   >  2:2944845/1‑148 (MQ=17)
                                     gCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAttt                                                   >  1:1040878/1‑148 (MQ=17)
                                         gATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGc                                               >  2:1127979/1‑148 (MQ=17)
                                         gATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGc                                               >  2:1128030/1‑148 (MQ=17)
                                          atatTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCt                                              >  1:2237017/1‑148 (MQ=17)
                                            atTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGa                                            >  2:2870975/1‑148 (MQ=17)
                                            atTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGa                                            >  2:1694144/1‑148 (MQ=17)
                                             ttAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaa                                           <  1:2571499/148‑1 (MQ=17)
                                                 aGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGaa                                       >  2:2672577/1‑148 (MQ=17)
                                                  ggAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGACAAATTTCCGCTGAAAGAAt                                      >  2:2709577/1‑148 (MQ=17)
                                                   gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt                                     >  2:254121/1‑148 (MQ=17)
                                                   gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt                                     <  2:585398/148‑1 (MQ=17)
                                                   gAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt                                     >  1:2603440/1‑148 (MQ=17)
                                                     aaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa                                   <  2:1650329/148‑1 (MQ=17)
                                                     aaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa                                   <  2:1650396/148‑1 (MQ=17)
                                                     aaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa                                   >  1:184032/1‑148 (MQ=17)
                                                     aaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa                                   <  1:151853/148‑1 (MQ=17)
                                                      aaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaaa                                  >  1:203232/1‑148 (MQ=17)
                                                       aaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaat                                 <  2:1785081/148‑1 (MQ=17)
                                                       aaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaat                                 <  2:54591/148‑1 (MQ=17)
                                                        atcatcACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaata                                >  1:3083659/1‑148 (MQ=17)
                                                        atcatcACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaata                                >  1:543498/1‑148 (MQ=17)
                                                         tcatcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaataa                               >  2:298440/1‑148 (MQ=17)
                                                          catcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaataat                              >  2:2146888/1‑148 (MQ=17)
                                                           atcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATc                             >  2:980969/1‑148 (MQ=17)
                                                           atcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATc                             >  2:980973/1‑148 (MQ=17)
                                                            tcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATcc                            >  1:1965360/1‑148 (MQ=17)
                                                             cacaACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCa                           >  1:1767176/1‑148 (MQ=17)
                                                              acaACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAg                          <  1:485117/148‑1 (MQ=17)
                                                                  cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCa                      <  2:1177012/148‑1 (MQ=21)
                                                                  cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCa                      <  2:1413782/148‑1 (MQ=21)
                                                                  cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCa                      >  2:1880130/1‑148 (MQ=21)
                                                                   tGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCAt                     <  1:1072149/148‑1 (MQ=21)
                                                                   tGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCAt                     >  1:2366679/1‑148 (MQ=21)
                                                                     aTGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGa                   <  1:1566793/148‑1 (MQ=21)
                                                                     aTGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGa                   <  1:1669645/148‑1 (MQ=21)
                                                                      tGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGAc                  <  2:1551458/148‑1 (MQ=21)
                                                                      tGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGAc                  >  1:2072657/1‑148 (MQ=21)
                                                                       gCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACa                 >  1:388487/1‑148 (MQ=21)
                                                                       gCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACa                 >  1:2161025/1‑148 (MQ=21)
                                                                        cGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAg                <  1:338114/148‑1 (MQ=21)
                                                                         gATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGt               <  1:3091334/148‑1 (MQ=21)
                                                                         gATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGt               <  1:275248/148‑1 (MQ=21)
                                                                          aTGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACCGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGtt              <  2:3074450/148‑1 (MQ=21)
                                                                          aTGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGtt              <  2:2131568/148‑1 (MQ=21)
                                                                           tGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTa             <  2:3296781/148‑1 (MQ=21)
                                                                             ggTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACg           >  2:2182135/1‑148 (MQ=21)
                                                                               ttGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCa         <  1:2056405/148‑1 (MQ=21)
                                                                                tGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCaa        <  2:505841/148‑1 (MQ=21)
                                                                                  ccAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATg      >  2:1466733/1‑148 (MQ=25)
                                                                                  ccAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATg      >  2:1466796/1‑148 (MQ=25)
                                                                                   cAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGa     >  1:2678465/1‑148 (MQ=25)
                                                                                   cAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGa     <  2:2503849/148‑1 (MQ=25)
                                                                                         |                                                                                                                                                
CTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTG  >  CP009273/1526518‑1526751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑