BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,548,144	G→T	100%	intergenic (‑49/+85)	adhP ← / ← maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,548,144	0	G	T	99.4%	588.7 / ‑4.2	178	intergenic (‑49/+85)	adhP/maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (96/81); total (97/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGC  >  CP009273/1547999‑1548288

acTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGatat                                                                                                                                                <  1:1691882/148‑1 (MQ=255)

  tCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                              <  1:539590/148‑1 (MQ=255)

  tCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                              <  2:2970455/148‑1 (MQ=255)

  tCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                              >  2:1544581/1‑148 (MQ=255)

  tCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                              >  2:1544651/1‑148 (MQ=255)

    cATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAt                                                                                                                                            <  2:1693432/148‑1 (MQ=255)

    cATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAt                                                                                                                                            <  2:2150674/148‑1 (MQ=255)

       tttCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCg                                                                                                                                         >  2:2809323/1‑148 (MQ=255)

        ttCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGc                                                                                                                                        <  2:2802921/148‑1 (MQ=255)

            gcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACt                                                                                                                                    >  2:2828171/1‑148 (MQ=255)

               gggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAcc                                                                                                                                 >  2:50649/1‑148 (MQ=255)

               gggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAcc                                                                                                                                 >  2:368154/1‑148 (MQ=255)

                  cTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                              <  2:1897792/148‑1 (MQ=255)

                    tCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTa                                                                                                                            <  2:2496798/148‑1 (MQ=255)

                     cGCCATGTTTCAGTGAGCGCAGTGTTTTATACGAAACGTCAACATGATTATCCTTCGTAACAACTGCAGACTTCATAGATCCTCCTTTTCGGATGATGTTCAGCATAGCAGGTGAGGCAAAAGATATTAATTCGCCACTACCAATTAt                                                                                                                           >  1:1098257/1‑148 (MQ=255)

                       ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATgg                                                                                                                         <  2:526899/148‑1 (MQ=255)

                        cATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                        >  1:798316/1‑148 (MQ=255)

                             ttCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATgaga                                                                                                                   >  2:1042649/1‑148 (MQ=255)

                                  tGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                              <  1:2809323/148‑1 (MQ=255)

                                   gAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaa                                                                                                             >  1:282784/1‑148 (MQ=255)

                                     gcgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCACATGATATTTATTCGCCACTACCCCGTATGGATGAGATCTGaaaa                                                                                                           >  1:1208716/1‑148 (MQ=255)

                                     gcgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaaa                                                                                                           <  2:2459945/148‑1 (MQ=255)

                                        cAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                        >  1:3035680/1‑148 (MQ=255)

                                          gtgtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGga                                                                                                      >  1:3297094/1‑148 (MQ=255)

                                           tgtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgag                                                                                                     >  2:1781725/1‑148 (MQ=255)

                                            gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgaga                                                                                                    >  1:1493324/1‑148 (MQ=255)

                                            gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgaga                                                                                                    <  2:2759268/148‑1 (MQ=255)

                                            gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgaga                                                                                                    <  2:2804150/148‑1 (MQ=255)

                                            gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgaga                                                                                                    >  1:153292/1‑148 (MQ=255)

                                             ttttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGagag                                                                                                   <  2:3209378/148‑1 (MQ=255)

                                               ttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                 <  1:910576/148‑1 (MQ=255)

                                               ttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                 <  2:2293665/148‑1 (MQ=255)

                                               ttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                 <  2:2645424/148‑1 (MQ=255)

                                               ttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                 <  2:2293593/148‑1 (MQ=255)

                                                 ataCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaa                                                                                               >  1:959400/1‑148 (MQ=255)

                                                 ataCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaa                                                                                               >  1:622739/1‑148 (MQ=255)

                                                  taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaaa                                                                                              <  2:1169232/148‑1 (MQ=255)

                                                  taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaaa                                                                                              <  1:1042649/148‑1 (MQ=255)

                                                  taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaaa                                                                                              <  1:1268599/148‑1 (MQ=255)

                                                   aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAt                                                                                             >  1:506289/1‑148 (MQ=255)

                                                   aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAt                                                                                             >  1:2473619/1‑148 (MQ=255)

                                                   aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAt                                                                                             >  2:2996804/1‑148 (MQ=255)

                                                   aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAt                                                                                             >  2:217416/1‑148 (MQ=255)

                                                      tAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGc                                                                                          >  1:1019995/1‑148 (MQ=255)

                                                         cGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCg                                                                                       >  2:2173798/1‑148 (MQ=255)

                                                          gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCgg                                                                                      <  1:30890/148‑1 (MQ=255)

                                                          gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCgg                                                                                      <  1:1143227/148‑1 (MQ=255)

                                                          gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCgg                                                                                      <  1:2794576/148‑1 (MQ=255)

                                                              aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGc                                                                                  >  1:1945003/1‑148 (MQ=255)

                                                               cATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGcc                                                                                 >  1:2755920/1‑148 (MQ=255)

                                                                  gatgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTc                                                                              <  1:3195372/148‑1 (MQ=255)

                                                                     gatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACt                                                                           >  2:253256/1‑148 (MQ=255)

                                                                         cTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAACAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCg                                                                       >  2:3196518/1‑148 (MQ=255)

                                                                          ttCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCgg                                                                      >  2:3299297/1‑148 (MQ=255)

                                                                          ttCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCgg                                                                      >  2:1746666/1‑148 (MQ=255)

                                                                               aacaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCa                                                                 >  2:1341624/1‑148 (MQ=255)

                                                                               aacaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCa                                                                 <  1:1779426/148‑1 (MQ=255)

                                                                               aacaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCa                                                                 >  2:2773586/1‑148 (MQ=255)

                                                                                acaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAg                                                                >  1:1976827/1‑148 (MQ=255)

                                                                                 caacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAgg                                                               >  1:2271902/1‑148 (MQ=255)

                                                                                 caacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACGACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAgg                                                               >  1:1156829/1‑148 (MQ=255)

                                                                                   acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCt                                                             >  2:227789/1‑148 (MQ=255)

                                                                                    cTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCtt                                                            <  2:1191873/148‑1 (MQ=255)

                                                                                      gCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAg                                                          >  1:2954084/1‑148 (MQ=255)

                                                                                         gCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATg                                                       >  2:1375212/1‑148 (MQ=255)

                                                                                          ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                      >  2:2833987/1‑148 (MQ=255)

                                                                                          ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                      <  2:2758649/148‑1 (MQ=255)

                                                                                          ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATgg                                                      >  1:2062782/1‑148 (MQ=255)

                                                                                           cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGa                                                     <  1:2908610/148‑1 (MQ=255)

                                                                                           cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGa                                                     <  1:821985/148‑1 (MQ=255)

                                                                                           cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGa                                                     >  1:995488/1‑148 (MQ=255)

                                                                                                tAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAc                                                >  1:2760469/1‑148 (MQ=255)

                                                                                                  gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcgg                                              >  2:2429060/1‑148 (MQ=255)

                                                                                                    tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcg                                            >  2:11309/1‑148 (MQ=255)

                                                                                                    tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcg                                            >  1:1221065/1‑148 (MQ=255)

                                                                                                     cctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGCGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcgg                                           >  2:2372979/1‑148 (MQ=255)

                                                                                                      ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTATCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                          <  2:263234/148‑1 (MQ=255)

                                                                                                      ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGt                                          <  1:2360794/148‑1 (MQ=255)

                                                                                                           tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                     >  2:1552527/1‑148 (MQ=255)

                                                                                                             tCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcgcg                                   <  1:2421153/148‑1 (MQ=255)

                                                                                                                gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa                                <  2:2179304/148‑1 (MQ=255)

                                                                                                                gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa                                <  2:69580/148‑1 (MQ=255)

                                                                                                                    atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCg                            >  2:1481951/1‑148 (MQ=255)

                                                                                                                     tgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                           >  2:927788/1‑148 (MQ=255)

                                                                                                                     tgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                           >  2:2335234/1‑148 (MQ=255)

                                                                                                                      gTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                          <  2:1389514/148‑1 (MQ=255)

                                                                                                                       ttCTGCATAGCAGGTGAGGCAACTGATATTTATTCGCCAATACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCt                         <  1:2154564/148‑1 (MQ=255)

                                                                                                                       ttCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCt                         <  1:687341/148‑1 (MQ=255)

                                                                                                                       ttCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCt                         <  1:954512/148‑1 (MQ=255)

                                                                                                                       ttCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCt                         <  2:434325/148‑1 (MQ=255)

                                                                                                                         cTGCATAGCAGGTGAGGCAAATGATATTTAGTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTg                       <  2:85714/148‑1 (MQ=255)

                                                                                                                            cATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCa                    >  1:865301/1‑148 (MQ=255)

                                                                                                                            cATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCa                    >  2:1647384/1‑148 (MQ=255)

                                                                                                                                gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                <  1:1392338/148‑1 (MQ=255)

                                                                                                                                 cAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAt               >  1:2576375/1‑148 (MQ=255)

                                                                                                                                   ggTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTa             >  1:2833647/1‑148 (MQ=255)

                                                                                                                                    gTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt            <  1:1341624/148‑1 (MQ=255)

                                                                                                                                     tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATc           <  2:3265629/148‑1 (MQ=255)

                                                                                                                                          cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCaa      <  1:1629256/148‑1 (MQ=255)

                                                                                                                                            aaTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATg    >  2:2083721/1‑148 (MQ=255)

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGC  >  CP009273/1547999‑1548288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`