Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,550,117 T→G 19.0% N35T (AAC→ACC)  sra ← stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,550,1170TG19.0% 402.4 / 125.0 221N35T (AAC→ACC) srastationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (105/74);  new base G (23/19);  total (128/93)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

Only 100 of 225 total aligned reads displayed.

TCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCC  >  CP009273/1549970‑1550264
                                                                                                                                                   |                                                                                                                                                   
  gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTa                                                                                                                                                   >  2:2778735/1‑148 (MQ=255)
  gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTa                                                                                                                                                   <  2:366457/148‑1 (MQ=255)
    cTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                                                                                                 >  2:3077005/1‑148 (MQ=255)
    cTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                                                                                                 <  1:699869/148‑1 (MQ=255)
      ttGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTta                                                                                                                                               <  1:2854933/148‑1 (MQ=255)
      ttGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTta                                                                                                                                               >  1:2914716/1‑148 (MQ=255)
       tGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtac                                                                                                                                              <  2:196255/148‑1 (MQ=255)
         cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtacta                                                                                                                                            >  2:1490022/1‑148 (MQ=255)
           ttACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                                                                                                          >  2:156233/1‑148 (MQ=255)
           ttACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACg                                                                                                                                          <  1:2796668/148‑1 (MQ=255)
              ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTg                                                                                                                                       >  2:1711570/1‑148 (MQ=255)
              ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTg                                                                                                                                       >  2:1116287/1‑148 (MQ=255)
               cGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTAATACGCTgg                                                                                                                                      >  1:1173788/1‑148 (MQ=255)
                 ccAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAt                                                                                                                                    <  2:2879835/148‑1 (MQ=255)
                 ccAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGGTGGGTTATTTACTACGCTGGAt                                                                                                                                    >  1:496957/1‑148 (MQ=255)
                  cAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAtt                                                                                                                                   <  2:2260029/148‑1 (MQ=255)
                     ggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGt                                                                                                                                >  2:2150133/1‑148 (MQ=255)
                     ggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGt                                                                                                                                >  2:485700/1‑148 (MQ=255)
                          cGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTGTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCt                                                                                                                           >  1:1450866/1‑148 (MQ=255)
                           ggCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCtt                                                                                                                          <  2:2113964/148‑1 (MQ=255)
                              cTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCgg                                                                                                                       <  2:2235907/148‑1 (MQ=255)
                                  aGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGGTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAc                                                                                                                   >  1:2779098/1‑148 (MQ=255)
                                   gTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACt                                                                                                                  <  2:90669/148‑1 (MQ=255)
                                   gTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACt                                                                                                                  >  2:108877/1‑148 (MQ=255)
                                      taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTGTTCTGCCGGTTGGGTTATTTTCTACGCTGGATTTGTAACCTTCGGTAACTagt                                                                                                               >  1:335337/1‑146 (MQ=255)
                                        aaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGGTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAtttt                                                                                                             >  1:1128904/1‑148 (MQ=255)
                                           cAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcg                                                                                                          <  1:2086048/148‑1 (MQ=255)
                                             gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                                                                                                        <  1:1145607/148‑1 (MQ=255)
                                             gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                                                                                                        >  2:468597/1‑148 (MQ=255)
                                              aTAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTg                                                                                                       <  2:115098/148‑1 (MQ=255)
                                                 aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGtt                                                                                                    >  2:3013097/1‑148 (MQ=255)
                                                    acaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                                                                                                 <  1:3308064/148‑1 (MQ=255)
                                                    acaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                                                                                                 <  1:3159848/148‑1 (MQ=255)
                                                     caaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCGGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaa                                                                                                >  1:1393108/1‑148 (MQ=255)
                                                        aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTGAGAAAtt                                                                                             >  1:2293437/1‑148 (MQ=255)
                                                         aacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAttt                                                                                            >  1:2979016/1‑148 (MQ=255)
                                                          acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt                                                                                           <  1:17544/148‑1 (MQ=255)
                                                          acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt                                                                                           <  1:1742593/148‑1 (MQ=255)
                                                           cacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa                                                                                          <  1:1828189/148‑1 (MQ=255)
                                                              cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATgg                                                                                       >  1:1809819/1‑148 (MQ=255)
                                                                ttGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc                                                                                     >  2:1377329/1‑148 (MQ=255)
                                                                ttGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc                                                                                     <  2:1767388/148‑1 (MQ=255)
                                                                 tGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcc                                                                                    >  1:710607/1‑148 (MQ=255)
                                                                   cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccag                                                                                  >  2:3169659/1‑148 (MQ=255)
                                                                     cAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtc                                                                                >  2:1926440/1‑148 (MQ=255)
                                                                       ggCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtcca                                                                              <  1:1163570/148‑1 (MQ=255)
                                                                         cAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAg                                                                            >  1:356031/1‑148 (MQ=255)
                                                                          aaTGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGa                                                                           <  2:1687448/148‑1 (MQ=255)
                                                                            tGGTTTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAat                                                                         <  2:1832343/148‑1 (MQ=255)
                                                                            tGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTTCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAat                                                                         >  1:1989237/1‑148 (MQ=255)
                                                                             ggTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAata                                                                        <  1:2778735/148‑1 (MQ=255)
                                                                                 tttAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGAc                                                                    >  1:2719496/1‑148 (MQ=255)
                                                                                     aTCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGc                                                                <  1:15916/148‑1 (MQ=255)
                                                                                     aTCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGc                                                                >  2:2474116/1‑148 (MQ=255)
                                                                                      tCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGcc                                                               <  1:1532969/148‑1 (MQ=255)
                                                                                       cGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCt                                                              >  1:799082/1‑148 (MQ=255)
                                                                                       cGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCt                                                              >  1:2956739/1‑148 (MQ=255)
                                                                                        gTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTg                                                             <  2:710700/148‑1 (MQ=255)
                                                                                         tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGa                                                            <  2:428438/148‑1 (MQ=255)
                                                                                         tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGa                                                            >  1:167105/1‑148 (MQ=255)
                                                                                         tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGa                                                            >  1:1837659/1‑148 (MQ=255)
                                                                                         tCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTATGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTTCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATTACGTGCCTGa                                                            >  1:344855/1‑148 (MQ=255)
                                                                                              gAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGtt                                                       <  1:1211149/148‑1 (MQ=255)
                                                                                                 gACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGa                                                    <  1:2058751/148‑1 (MQ=255)
                                                                                                 gACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGa                                                    <  2:1431995/148‑1 (MQ=255)
                                                                                                     gATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTc                                                <  1:2686259/148‑1 (MQ=255)
                                                                                                      aTGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCa                                               >  1:1063967/1‑148 (MQ=255)
                                                                                                       tGGTTATGAATTACTTTTCAGCGGGGCGTTTTGTGCCGGTTGGGGTATTTAATACGCTGGATTTTTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAATTTATGGTCCAGTCCAACAATATGACGTGCCTGACGGGTCGAGTTcct                                              >  1:1473417/1‑146 (MQ=255)
                                                                                                        ggTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCAtt                                             >  2:47852/1‑148 (MQ=255)
                                                                                                          ttATGAATTACTTTTCAGCGGGGCGTTTTGTGCCGGTTGGGGTATTTAATACGCTGGATTTGTCACCATAGGTAACTATTTTTCGCTGGTTAGAAATTTTATTGTCCAGTCCAAGAATATTTCGTGCCTGACGGTTCGATTTGAtttt                                           >  1:1227940/1‑148 (MQ=255)
                                                                                                          ttATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCAtttt                                           >  2:244058/1‑148 (MQ=255)
                                                                                                           tATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTa                                          <  1:3225899/148‑1 (MQ=255)
                                                                                                           tATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTa                                          >  1:3020963/1‑148 (MQ=255)
                                                                                                            aTGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTaa                                         >  2:66865/1‑148 (MQ=255)
                                                                                                            aTGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTAATACGCTGGGTTTGTCACCTTCGGTAACTATTTTTCGCTGGGTAGAAATTTTATTGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTAATTTTaa                                         >  1:2237607/1‑148 (MQ=255)
                                                                                                              gAATTACTTTTCAGCGGGGCGTTTTGTGCCGGGTGGGTTATTTAATAAGCGGGCTTTTTCACCTTCGGTAACAATTTTTCGCGGGGTAGAAATTTTATGGTGCAGTACAAGAATATTAAGTTCCTGACGGTTAGATTTGAtttttaat                                       >  1:3078365/1‑144 (MQ=255)
                                                                                                              gAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAAct                                       <  1:551389/148‑1 (MQ=255)
                                                                                                              gAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAAct                                       >  1:3279285/1‑148 (MQ=255)
                                                                                                                aTTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcc                                     <  2:344129/148‑1 (MQ=255)
                                                                                                                 ttACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAActcct                                    <  1:1711570/148‑1 (MQ=255)
                                                                                                                 ttACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTAGATTTCATTTTAActcct                                    >  2:2662310/1‑148 (MQ=255)
                                                                                                                   aCTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCa                                  <  1:989295/148‑1 (MQ=255)
                                                                                                                     ttttCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTACTCAAt                                >  2:304080/1‑148 (MQ=255)
                                                                                                                      tttCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACAATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCGGACGGTTCGATTTCATTTTAACTACTCAATc                               >  1:475058/1‑148 (MQ=255)
                                                                                                                           gCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTa                          >  2:768901/1‑148 (MQ=255)
                                                                                                                              gggCGTTTTCTTCCGGTTGGGTTATTTACTACGCTTGATTTTTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGTCGTGCCTGACGGTTCGATTTAATTTTATCTCATCAATCCGGTAGcg                       >  1:2095257/1‑147 (MQ=255)
                                                                                                                               ggCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTa                      >  2:387644/1‑148 (MQ=255)
                                                                                                                               ggCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTa                      >  2:3282378/1‑148 (MQ=255)
                                                                                                                                 cGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCTGTAACTATTTTGCGCTTGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGt                    <  1:1972921/148‑1 (MQ=255)
                                                                                                                                 cGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGt                    >  2:2719972/1‑148 (MQ=255)
                                                                                                                                  gTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGtt                   <  2:2431268/148‑1 (MQ=255)
                                                                                                                                  gTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGtt                   <  2:2431134/148‑1 (MQ=255)
                                                                                                                                   ttttCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGttt                  >  1:1658345/1‑148 (MQ=255)
                                                                                                                                    tttCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGtttt                 <  1:750808/148‑1 (MQ=255)
                                                                                                                                    tttCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGtttt                 >  1:1007773/1‑148 (MQ=255)
                                                                                                                                             gTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGacaac        <  2:1219099/148‑1 (MQ=255)
                                                                                                                                             gTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGacaac        <  1:1587693/148‑1 (MQ=255)
                                                                                                                                                 ggTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCg    >  2:2049064/1‑148 (MQ=255)
                                                                                                                                                   ttATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGcc  <  1:2540140/148‑1 (MQ=255)
                                                                                                                                                   ttATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGcc  >  2:1337125/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAATCCTGTAGCTAGTTTTAAGGACAACATCGCC  >  CP009273/1549970‑1550264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑