Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,551,620 C→A 38.6% D226Y (GAC→TAC)  ddpF ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,551,6200CA38.6% 256.5 / 189.7 196D226Y (GAC→TAC) ddpFD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (54/66);  new base A (25/50);  total (79/117)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAAC  >  CP009273/1551477‑1551767
                                                                                                                                               |                                                                                                                                                   
gCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGccc                                                                                                                                                 >  1:1818834/1‑148 (MQ=255)
 ccTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATAGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCa                                                                                                                                                >  2:2477980/1‑148 (MQ=255)
   ttGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGt                                                                                                                                              >  1:2887665/1‑148 (MQ=255)
   ttGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGt                                                                                                                                              >  1:1547374/1‑148 (MQ=255)
      cGGCAACGTGCGGTTTCCCGGCAGATCCGGTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCt                                                                                                                                           <  2:3063053/148‑1 (MQ=255)
       ggCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTa                                                                                                                                          <  2:1454659/148‑1 (MQ=255)
        gCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCGGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                                         >  2:3152031/1‑148 (MQ=255)
        gCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                                         >  1:209997/1‑148 (MQ=255)
        gCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                                         >  1:2064175/1‑148 (MQ=255)
        gCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                                         >  1:1891785/1‑148 (MQ=255)
         cAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACa                                                                                                                                        >  2:2803128/1‑148 (MQ=255)
           aCGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAAt                                                                                                                                      >  1:877797/1‑148 (MQ=255)
            cGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATc                                                                                                                                     <  2:2910263/148‑1 (MQ=255)
            cGTGCGGTCTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATc                                                                                                                                     <  1:1833953/148‑1 (MQ=255)
              tGCGGTTTCCCTGCATATCAGTTTTACGTTATGCCCATTCTTCCTCAAGCGTTTTATCAATGGCGGGGAGGGATTCCAGCAATAATAGGGTGTATTGATGTGCAGGTGCCGTCAGCACTTGCTGCGCGTTGCCCAGTTCTACAATCTg                                                                                                                                   <  2:588030/148‑1 (MQ=255)
              tGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                                                                                                   <  1:2311082/148‑1 (MQ=255)
               gCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGc                                                                                                                                  >  2:501563/1‑148 (MQ=255)
                cGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGcc                                                                                                                                 >  1:1802293/1‑148 (MQ=255)
                    ttCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                                                                                             <  2:3034712/148‑1 (MQ=255)
                            aGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCa                                                                                                                     <  2:785314/148‑1 (MQ=255)
                             gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCAc                                                                                                                    >  2:1817438/1‑148 (MQ=255)
                               tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCg                                                                                                                  >  2:1255605/1‑148 (MQ=255)
                               tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCg                                                                                                                  >  1:1939693/1‑148 (MQ=255)
                                ccGTTTTACGTCATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                                                                                 <  2:2105725/148‑1 (MQ=255)
                                ccGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                                                                                 >  2:1195450/1‑148 (MQ=255)
                                 cGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGcc                                                                                                                <  1:2021544/148‑1 (MQ=255)
                                    tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                                                                                             >  1:2045925/1‑148 (MQ=255)
                                       aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGa                                                                                                          <  2:2435242/148‑1 (MQ=255)
                                         gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc                                                                                                        <  2:135386/148‑1 (MQ=255)
                                          tAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg                                                                                                       <  1:815942/148‑1 (MQ=255)
                                           aaTGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGc                                                                                                      <  2:1915658/148‑1 (MQ=255)
                                            aTGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                                                                                     <  1:60900/148‑1 (MQ=255)
                                                ccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata                                                                                                 <  1:3267999/148‑1 (MQ=255)
                                                ccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata                                                                                                 <  2:1517802/148‑1 (MQ=255)
                                                     cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                                                                                            >  2:2785520/1‑148 (MQ=255)
                                                     cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                                                                                            >  2:378657/1‑148 (MQ=255)
                                                     cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                                                                                            <  2:167553/148‑1 (MQ=255)
                                                       tcctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTa                                                                                          >  2:2083467/1‑148 (MQ=255)
                                                        cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                                                                                         >  2:1491610/1‑148 (MQ=255)
                                                        cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                                                                                         <  1:2615477/148‑1 (MQ=255)
                                                        cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                                                                                         <  1:1664987/148‑1 (MQ=255)
                                                         ctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATc                                                                                        <  1:537436/148‑1 (MQ=255)
                                                            cAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                                                                                     <  2:886517/148‑1 (MQ=255)
                                                             aGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                                                    >  1:1426624/1‑148 (MQ=255)
                                                             aGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                                                    <  1:1713763/148‑1 (MQ=255)
                                                              gCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCga                                                                                   >  1:1444348/1‑148 (MQ=255)
                                                               cGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                                                                                  <  2:1393048/148‑1 (MQ=255)
                                                               cGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                                                                                  <  1:2532347/148‑1 (MQ=255)
                                                                    tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtt                                                                             <  2:542364/148‑1 (MQ=255)
                                                                     aTCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg                                                                            <  1:3150973/148‑1 (MQ=255)
                                                                     aTCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg                                                                            <  2:1547374/148‑1 (MQ=255)
                                                                      tCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgt                                                                           >  1:891360/1‑148 (MQ=255)
                                                                       cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtg                                                                          <  1:2787207/148‑1 (MQ=255)
                                                                       cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtg                                                                          >  2:2187720/1‑148 (MQ=255)
                                                                       cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtg                                                                          >  1:1593201/1‑148 (MQ=255)
                                                                           ggCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa                                                                      <  1:1795731/148‑1 (MQ=255)
                                                                           ggCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa                                                                      >  1:930724/1‑148 (MQ=255)
                                                                           ggCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa                                                                      <  1:2814742/148‑1 (MQ=255)
                                                                             cGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAAt                                                                    >  2:2145486/1‑148 (MQ=255)
                                                                               gggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCa                                                                  >  2:872996/1‑148 (MQ=255)
                                                                                    ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGcaca                                                             >  1:3045964/1‑148 (MQ=255)
                                                                                     gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACAt                                                            <  2:2064175/148‑1 (MQ=255)
                                                                                     gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACAt                                                            <  2:1891665/148‑1 (MQ=255)
                                                                                     gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACAt                                                            >  1:1095178/1‑148 (MQ=255)
                                                                                     gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACAt                                                            >  2:1219737/1‑148 (MQ=255)
                                                                                        tCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCGGTTCTACAATCTGCCCGAGATACATCACCACCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAgg                                                         >  2:1482756/1‑148 (MQ=255)
                                                                                         ccAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGt                                                        >  2:1975211/1‑148 (MQ=255)
                                                                                         ccAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGt                                                        <  1:675716/148‑1 (MQ=255)
                                                                                             cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGc                                                    >  1:1249637/1‑148 (MQ=255)
                                                                                              aataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGcc                                                   <  2:2628145/148‑1 (MQ=255)
                                                                                                taatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCg                                                 >  2:2875558/1‑148 (MQ=255)
                                                                                                   tCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                                              <  2:1746933/148‑1 (MQ=255)
                                                                                                   tCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                                              <  1:1702175/148‑1 (MQ=255)
                                                                                                   tCGGGTGTATGGAAGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                                              <  2:249750/148‑1 (MQ=255)
                                                                                                    cGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGAt                                             <  2:3122515/148‑1 (MQ=255)
                                                                                                      ggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGAttt                                           >  2:2615405/1‑148 (MQ=255)
                                                                                                        tgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTc                                         <  1:1316849/148‑1 (MQ=255)
                                                                                                             ggATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTa                                    <  2:631419/148‑1 (MQ=255)
                                                                                                              gATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAg                                   >  2:100607/1‑148 (MQ=255)
                                                                                                                 gtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGt                                <  2:2852345/148‑1 (MQ=255)
                                                                                                                 gtgCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGt                                <  1:3229713/148‑1 (MQ=255)
                                                                                                                 gtgCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGt                                <  1:2014892/148‑1 (MQ=255)
                                                                                                                  tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGtt                               <  1:1017911/148‑1 (MQ=255)
                                                                                                                  tgCAGGTGCCGTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGtt                               >  1:1161425/1‑148 (MQ=255)
                                                                                                                        tGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGt                         <  1:1078379/148‑1 (MQ=255)
                                                                                                                          ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTaa                       <  2:2313060/148‑1 (MQ=255)
                                                                                                                          ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTaa                       <  1:1405833/148‑1 (MQ=255)
                                                                                                                            gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAt                     >  1:440487/1‑148 (MQ=255)
                                                                                                                            gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAt                     >  1:2105814/1‑148 (MQ=255)
                                                                                                                            gTCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAt                     <  2:1802293/148‑1 (MQ=255)
                                                                                                                             tCAGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAtt                    >  1:1303583/1‑148 (MQ=255)
                                                                                                                              cAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTg                   >  2:559409/1‑148 (MQ=255)
                                                                                                                               aGCACCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGa                  <  1:1769438/148‑1 (MQ=255)
                                                                                                                                gCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCAGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCTAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAg                 <  2:2052792/148‑1 (MQ=255)
                                                                                                                                  aCCTGCTGCGCGTAGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGa               <  2:2699082/148‑1 (MQ=255)
                                                                                                                                   ccTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGAt              <  2:1513913/148‑1 (MQ=255)
                                                                                                                                   ccTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGAt              <  2:1325181/148‑1 (MQ=255)
                                                                                                                                      gctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTg           <  2:1444348/148‑1 (MQ=255)
                                                                                                                                      gctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTg           <  1:1528479/148‑1 (MQ=255)
                                                                                                                                          cgcgTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGcc       <  2:930724/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAAC  >  CP009273/1551477‑1551767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑