Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,557,309 C→A 19.8% D9Y (GAT→TAT)  ddpX ← D‑ala‑D‑ala dipeptidase, Zn‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,557,3090CA19.8% 732.7 / 116.9 267D9Y (GAT→TAT) ddpXD‑ala‑D‑ala dipeptidase, Zn‑dependent
Reads supporting (aligned to +/- strand):  ref base C (110/104);  new base A (28/25);  total (138/129)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 273 total aligned reads displayed.

GGGCGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTG  >  CP009273/1557162‑1557456
                                                                                                                                                   |                                                                                                                                                   
gggCGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATc                                                                                                                                                     >  2:2865307/1‑148 (MQ=255)
      tGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAg                                                                                                                                               >  1:1895921/1‑148 (MQ=255)
      tGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAg                                                                                                                                               >  2:2470972/1‑148 (MQ=255)
           aTACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCgg                                                                                                                                          >  2:385218/1‑148 (MQ=255)
           aTACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCgg                                                                                                                                          >  1:2311131/1‑148 (MQ=255)
                tttCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTa                                                                                                                                     <  2:3236678/148‑1 (MQ=255)
                  tCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATc                                                                                                                                   <  2:1824043/148‑1 (MQ=255)
                      cAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGAc                                                                                                                               <  2:1365806/148‑1 (MQ=255)
                        gcgcGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACAt                                                                                                                             >  2:2692007/1‑148 (MQ=255)
                          gcgGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATaa                                                                                                                           <  1:1694863/148‑1 (MQ=255)
                          gcgGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATaa                                                                                                                           <  1:1683980/148‑1 (MQ=255)
                            ggTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAAc                                                                                                                         <  1:2290391/148‑1 (MQ=255)
                             gTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACg                                                                                                                        >  1:707419/1‑148 (MQ=255)
                              tAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACgg                                                                                                                       >  1:2953106/1‑148 (MQ=255)
                                aTCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGtt                                                                                                                     >  2:2982624/1‑148 (MQ=255)
                                 tCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTc                                                                                                                    <  1:695752/148‑1 (MQ=255)
                                 tCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTc                                                                                                                    >  1:892244/1‑148 (MQ=255)
                                 tCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTc                                                                                                                    <  1:3193585/148‑1 (MQ=255)
                                    cATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTg                                                                                                                 >  2:1522167/1‑148 (MQ=255)
                                       ccTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGtat                                                                                                              <  1:426395/148‑1 (MQ=255)
                                       ccTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGtat                                                                                                              <  1:2091467/148‑1 (MQ=255)
                                       ccTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGtat                                                                                                              >  1:141561/1‑148 (MQ=255)
                                          tgtgTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAg                                                                                                           <  2:854683/148‑1 (MQ=255)
                                             gtAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACa                                                                                                        <  1:1435193/148‑1 (MQ=255)
                                              tAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaa                                                                                                       >  2:753723/1‑148 (MQ=255)
                                              tAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaa                                                                                                       >  1:1084310/1‑148 (MQ=255)
                                                aCAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaaaa                                                                                                     >  1:2752133/1‑148 (MQ=255)
                                                   gACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAATAAtt                                                                                                  >  1:2635886/1‑148 (MQ=255)
                                                    aCAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtg                                                                                                 >  1:2907730/1‑148 (MQ=255)
                                                     cAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgc                                                                                                >  1:227759/1‑148 (MQ=255)
                                                          cgcTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTgcgc                                                                                           <  1:259018/148‑1 (MQ=255)
                                                           gcTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCt                                                                                          <  1:1522167/148‑1 (MQ=255)
                                                              tGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTc                                                                                       >  2:712761/1‑148 (MQ=255)
                                                                 tGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTGATTTCGATCTCCAGATCAGGGAAGATTACGTCTAAATCTACCAGTTCGGTGGTATCAGACATAAACGGTTCCTGTATAAGACAAAAATTGCTTCGCTTTCCGc                                                                                    <  2:1230481/148‑1 (MQ=255)
                                                                 tGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGc                                                                                    >  2:2147647/1‑148 (MQ=255)
                                                                  gATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCt                                                                                   >  1:2985742/1‑148 (MQ=255)
                                                                  gATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCt                                                                                   >  1:1993460/1‑148 (MQ=255)
                                                                  gATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCt                                                                                   >  2:2774085/1‑148 (MQ=255)
                                                                     aaaTAGCTTTACCTGTGATGTTGTCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTAt                                                                                >  1:1517433/1‑148 (MQ=255)
                                                                     aaaTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTAt                                                                                >  1:715624/1‑148 (MQ=255)
                                                                       aTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGc                                                                              <  2:2976796/148‑1 (MQ=255)
                                                                        tAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCa                                                                             >  2:775052/1‑148 (MQ=255)
                                                                          gCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGa                                                                           <  1:3135363/148‑1 (MQ=255)
                                                                          gCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGa                                                                           <  1:2152298/148‑1 (MQ=255)
                                                                          gCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGa                                                                           >  1:611421/1‑148 (MQ=255)
                                                                                 cTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatg                                                                    >  2:1467145/1‑148 (MQ=255)
                                                                                  tgtgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgc                                                                   <  1:1062979/148‑1 (MQ=255)
                                                                                   gtgAGGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgcc                                                                  <  2:2541575/148‑1 (MQ=255)
                                                                                     gATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                >  1:2542795/1‑148 (MQ=255)
                                                                                     gATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                >  2:2846697/1‑148 (MQ=255)
                                                                                     gATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                >  2:2846826/1‑148 (MQ=255)
                                                                                     gATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccat                                                                >  2:1196265/1‑148 (MQ=255)
                                                                                        gTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCatgccatgcc                                                             >  1:1750166/1‑148 (MQ=255)
                                                                                         ttATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCg                                                            >  2:2961248/1‑148 (MQ=255)
                                                                                          tATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCgg                                                           >  2:1666651/1‑148 (MQ=255)
                                                                                            tCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGa                                                         <  1:672113/148‑1 (MQ=255)
                                                                                            tCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGa                                                         <  1:1556001/148‑1 (MQ=255)
                                                                                             cAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                        >  1:1010455/1‑148 (MQ=255)
                                                                                              aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATa                                                       <  1:1126993/148‑1 (MQ=255)
                                                                                              aGCGCAGGCGTATTTCAATTAGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATa                                                       >  2:609129/1‑148 (MQ=255)
                                                                                               gcgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATaa                                                      <  1:2722354/148‑1 (MQ=255)
                                                                                                cgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAg                                                     <  2:1066923/148‑1 (MQ=255)
                                                                                                cgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAg                                                     <  2:2722478/148‑1 (MQ=255)
                                                                                                   aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAgcgc                                                  <  2:1895921/148‑1 (MQ=255)
                                                                                                    ggCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGcc                                                 >  2:645965/1‑148 (MQ=255)
                                                                                                        tATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTATATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGaa                                             <  2:3187318/148‑1 (MQ=255)
                                                                                                        tATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGaa                                             >  2:2775924/1‑148 (MQ=255)
                                                                                                          tttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                           <  1:1560347/148‑1 (MQ=255)
                                                                                                          tttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                           >  2:2239162/1‑148 (MQ=255)
                                                                                                           ttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGc                                          >  2:1473005/1‑148 (MQ=255)
                                                                                                           ttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGc                                          >  1:2451405/1‑148 (MQ=255)
                                                                                                            tCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCt                                         <  2:2100836/148‑1 (MQ=255)
                                                                                                             cAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTg                                        <  2:2893364/148‑1 (MQ=255)
                                                                                                             cAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTg                                        >  1:2036077/1‑148 (MQ=255)
                                                                                                               aTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGc                                      <  1:1477312/148‑1 (MQ=255)
                                                                                                                   gATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaa                                  <  2:3122173/148‑1 (MQ=255)
                                                                                                                   gATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaa                                  <  2:1084310/148‑1 (MQ=255)
                                                                                                                    aTCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaaa                                 <  1:385218/148‑1 (MQ=255)
                                                                                                                     tctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAg                                >  1:3313600/1‑148 (MQ=255)
                                                                                                                      ctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGt                               <  1:1272171/148‑1 (MQ=255)
                                                                                                                         cAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtat                            >  2:2412271/1‑148 (MQ=255)
                                                                                                                                gggAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttttt                     >  2:2602152/1‑148 (MQ=255)
                                                                                                                                 ggAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTa                    >  2:2522155/1‑148 (MQ=255)
                                                                                                                                 ggAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTa                    >  2:484043/1‑148 (MQ=255)
                                                                                                                                 ggAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTa                    >  2:56738/1‑148 (MQ=255)
                                                                                                                                  gAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                   >  2:2943996/1‑148 (MQ=255)
                                                                                                                                  gAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                   >  2:1580482/1‑148 (MQ=255)
                                                                                                                                     gATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACtt                <  2:1911802/148‑1 (MQ=255)
                                                                                                                                      aTTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACttt               <  1:1323550/148‑1 (MQ=255)
                                                                                                                                        tACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTg             <  1:3019911/148‑1 (MQ=255)
                                                                                                                                        tACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTg             <  2:2311131/148‑1 (MQ=255)
                                                                                                                                          cGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGAt           <  1:1634411/148‑1 (MQ=255)
                                                                                                                                          cGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGAt           <  1:2933995/148‑1 (MQ=255)
                                                                                                                                            gCTAAATCAACCAGCTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCa         <  2:508900/148‑1 (MQ=255)
                                                                                                                                             cTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCaa        <  2:2029823/148‑1 (MQ=255)
                                                                                                                                              taaataaaCCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAAc       <  1:2692007/148‑1 (MQ=255)
                                                                                                                                              tAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAAc       <  1:2881456/148‑1 (MQ=255)
                                                                                                                                                  tCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATTTTTTTTAACTTTTGATCAACAttt   <  1:517878/148‑1 (MQ=255)
                                                                                                                                                  tCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAttt   >  2:1403880/1‑148 (MQ=255)
                                                                                                                                                  tCAACCAGTTCGGAGGTATCCGACATAAACCGTTCCTGAATAAGACAAAAATTGCTGCGCTTTACGCTTATGCAGATCTCATGCCAAGCCGCCATAATCGCCAGAATGCTGTCTTAAAGTTATATTTTTTAAATTTTGATCAACAttt   >  1:24312/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGCGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTG  >  CP009273/1557162‑1557456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑