Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,570,428 G→T 92.6% I483I (ATC→ATA)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,570,4280GT92.6% 582.4 / 38.2 218I483I (ATC→ATA) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base G (11/5);  new base T (123/79);  total (134/84)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 224 total aligned reads displayed.

TACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGG  >  CP009273/1570283‑1570572
                                                                                                                                                 |                                                                                                                                                
taCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGatat                                                                                                                                                >  1:1159343/1‑148 (MQ=255)
taCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGatat                                                                                                                                                <  1:3152760/148‑1 (MQ=255)
taCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGatat                                                                                                                                                >  2:2032830/1‑148 (MQ=255)
taCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGatat                                                                                                                                                <  1:553407/148‑1 (MQ=255)
    tGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATCTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGAGGGGCTATATtg                                                                                                                                            >  1:1784340/1‑148 (MQ=255)
      aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgtg                                                                                                                                          >  2:421991/1‑148 (MQ=255)
       tACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTgg                                                                                                                                         >  2:871239/1‑148 (MQ=255)
       tACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTgg                                                                                                                                         <  1:1644427/148‑1 (MQ=255)
            aTTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGa                                                                                                                                    <  2:332359/148‑1 (MQ=255)
              tCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACa                                                                                                                                  >  1:3092174/1‑148 (MQ=255)
                  tCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATa                                                                                                                              <  2:2360588/148‑1 (MQ=255)
                   ccAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAg                                                                                                                             >  1:1135728/1‑148 (MQ=255)
                        tATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtc                                                                                                                        <  1:1564801/148‑1 (MQ=255)
                           tGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcata                                                                                                                     >  2:1591519/1‑148 (MQ=255)
                           tGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcata                                                                                                                     >  1:415251/1‑148 (MQ=255)
                           tGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcata                                                                                                                     <  1:2911118/148‑1 (MQ=255)
                              ggATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtc                                                                                                                  <  2:1971471/148‑1 (MQ=255)
                                 tatCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtcata                                                                                                               <  2:960886/148‑1 (MQ=255)
                                   tCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATAcc                                                                                                             >  1:135832/1‑148 (MQ=255)
                                      cGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCa                                                                                                          >  1:593786/1‑148 (MQ=255)
                                       gTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCAc                                                                                                         >  1:1238604/1‑148 (MQ=255)
                                        tAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                        >  2:1333703/1‑148 (MQ=255)
                                         aaTCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGc                                                                                                       >  1:1810298/1‑148 (MQ=255)
                                               atatCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCa                                                                                                 <  2:2594715/148‑1 (MQ=255)
                                                 atCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAtt                                                                                               >  2:764501/1‑148 (MQ=255)
                                                 atCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAtt                                                                                               >  2:1650606/1‑148 (MQ=255)
                                                       aaTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                         >  1:756576/1‑148 (MQ=255)
                                                         tatTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGacac                                                                                       <  2:167817/148‑1 (MQ=255)
                                                            tCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACAtt                                                                                    >  1:1282143/1‑148 (MQ=255)
                                                             cccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTa                                                                                   <  2:473100/148‑1 (MQ=255)
                                                             cccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTa                                                                                   <  2:2137758/148‑1 (MQ=255)
                                                              ccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTAc                                                                                  >  1:2338542/1‑148 (MQ=255)
                                                                 ccATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGcc                                                                               >  1:1587325/1‑148 (MQ=255)
                                                                   aTAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAg                                                                             >  2:913541/1‑148 (MQ=255)
                                                                   aTAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAg                                                                             >  2:913364/1‑148 (MQ=255)
                                                                    tAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGTGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGTCAGc                                                                            >  1:3081735/1‑148 (MQ=255)
                                                                    tAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGc                                                                            >  2:1077809/1‑148 (MQ=255)
                                                                    tAGTAACGGTTATAACCTGCTGTAATCAGTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGc                                                                            >  2:2985272/1‑148 (MQ=255)
                                                                       tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCtca                                                                         >  2:1847171/1‑146 (MQ=255)
                                                                       tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTaa                                                                         >  2:3284219/1‑148 (MQ=255)
                                                                       tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTaa                                                                         >  2:448456/1‑148 (MQ=255)
                                                                        aaCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAAt                                                                        >  2:2394383/1‑148 (MQ=255)
                                                                        aaCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAAt                                                                        >  2:2169728/1‑148 (MQ=255)
                                                                         aCGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATg                                                                       >  2:2621302/1‑148 (MQ=255)
                                                                            gTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGa                                                                    <  2:85627/148‑1 (MQ=255)
                                                                             ttATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGAt                                                                   >  2:628709/1‑148 (MQ=255)
                                                                             ttATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGAt                                                                   <  1:2303747/148‑1 (MQ=255)
                                                                              tataACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATc                                                                  <  1:2959946/148‑1 (MQ=255)
                                                                                taACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCg                                                                >  2:1747597/1‑148 (MQ=255)
                                                                                taACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCg                                                                >  1:1674678/1‑148 (MQ=255)
                                                                                 aaCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                               <  2:2689732/148‑1 (MQ=255)
                                                                                 aaCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                               >  2:2192238/1‑148 (MQ=255)
                                                                                      gctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCatat                                                          <  1:985941/148‑1 (MQ=255)
                                                                                       ctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCAtata                                                         <  2:2801201/148‑1 (MQ=255)
                                                                                       ctgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCAtata                                                         <  1:1010519/148‑1 (MQ=255)
                                                                                        tgTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATAc                                                        >  2:632326/1‑148 (MQ=255)
                                                                                             tCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgt                                                   >  2:1354191/1‑148 (MQ=255)
                                                                                               aTTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtgt                                                 >  2:436383/1‑148 (MQ=255)
                                                                                                 tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                               >  2:328787/1‑148 (MQ=255)
                                                                                                  gAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGt                                              <  1:2139396/148‑1 (MQ=255)
                                                                                                     gTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGt                                           >  1:2216060/1‑148 (MQ=255)
                                                                                                      tttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTc                                          >  1:1960637/1‑148 (MQ=255)
                                                                                                      tttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTc                                          >  1:879784/1‑148 (MQ=255)
                                                                                                       ttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                         >  2:1936276/1‑148 (MQ=255)
                                                                                                       ttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                         >  1:2718238/1‑148 (MQ=255)
                                                                                                         gATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAt                                       <  2:2491218/148‑1 (MQ=255)
                                                                                                          aTTAGCAAAAATATTCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAtt                                      >  2:2443871/1‑148 (MQ=255)
                                                                                                          aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAtt                                      <  2:53234/148‑1 (MQ=255)
                                                                                                          aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAtt                                      <  2:2305094/148‑1 (MQ=255)
                                                                                                           ttAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTc                                     <  1:2777338/148‑1 (MQ=255)
                                                                                                           ttAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTc                                     >  1:2967212/1‑148 (MQ=255)
                                                                                                            tAGCAAAAATATCCCAGTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                    <  1:2032830/148‑1 (MQ=255)
                                                                                                             aGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTccc                                   <  2:681220/148‑1 (MQ=255)
                                                                                                              gCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCa                                  <  1:2883713/148‑1 (MQ=255)
                                                                                                                   aaTATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcgg                             >  2:15129/1‑148 (MQ=255)
                                                                                                                    atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcggc                            >  2:1893391/1‑148 (MQ=255)
                                                                                                                    atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcggc                            >  2:224178/1‑148 (MQ=255)
                                                                                                                    atatCCCATTCCGTCATAAAGCGCGGGGATAGATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcggc                            >  2:2497496/1‑148 (MQ=255)
                                                                                                                      atCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                          <  1:448456/148‑1 (MQ=255)
                                                                                                                       tCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                         >  1:429754/1‑148 (MQ=255)
                                                                                                                       tCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                         <  1:2529244/148‑1 (MQ=255)
                                                                                                                        cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGccttt                        <  1:3035106/148‑1 (MQ=255)
                                                                                                                          cATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttac                      >  2:720724/1‑148 (MQ=255)
                                                                                                                           aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacc                     <  2:307440/148‑1 (MQ=255)
                                                                                                                           aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacc                     >  2:947372/1‑148 (MQ=255)
                                                                                                                           aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacc                     <  2:2895168/148‑1 (MQ=255)
                                                                                                                            ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                    >  1:3242394/1‑148 (MQ=255)
                                                                                                                              ccGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttt                  <  1:1441123/148‑1 (MQ=255)
                                                                                                                                   aTAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGt             <  2:3199475/148‑1 (MQ=255)
                                                                                                                                    tAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTa            >  1:2177529/1‑148 (MQ=255)
                                                                                                                                        gcgcGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatg        >  1:3140140/1‑148 (MQ=255)
                                                                                                                                        gcgcGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGACGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATTGTAAatg        >  2:573246/1‑148 (MQ=255)
                                                                                                                                         cgcgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGAATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg       >  2:1775546/1‑148 (MQ=255)
                                                                                                                                          gcgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggt      <  1:3167828/148‑1 (MQ=255)
                                                                                                                                           cgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggta     <  2:1074297/148‑1 (MQ=255)
                                                                                                                                            ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtat    <  2:1039499/148‑1 (MQ=255)
                                                                                                                                            ggggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtat    <  2:2339450/148‑1 (MQ=255)
                                                                                                                                             gggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatg   >  2:2626270/1‑148 (MQ=255)
                                                                                                                                              ggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg  <  1:2387704/148‑1 (MQ=255)
                                                                                                                                              ggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg  <  2:2860249/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGG  >  CP009273/1570283‑1570572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑