Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,769,763 T→A 89.2% intergenic (+62/‑81) aroD → / → ydiF 3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,769,7630TA89.2% 550.9 / 48.7 185intergenic (+62/‑81)aroD/ydiF3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (11/9);  new base A (101/64);  total (112/73)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 192 total aligned reads displayed.

GGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769618‑1769907
                                                                                                                                                 |                                                                                                                                                
ggTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTtaa                                                                                                                                                <  1:710058/148‑1 (MQ=255)
ggTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaa                                                                                                                                                <  2:2676089/148‑1 (MQ=255)
ggTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaa                                                                                                                                                <  2:308634/148‑1 (MQ=255)
  tGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaata                                                                                                                                              <  1:1007529/148‑1 (MQ=255)
  tGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaata                                                                                                                                              <  2:2205915/148‑1 (MQ=255)
      gTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATaaaaa                                                                                                                                          >  2:2744383/1‑148 (MQ=255)
       tAAAAAAAGCGTCTGCGCAAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAat                                                                                                                                         <  2:2440459/148‑1 (MQ=255)
         aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAatat                                                                                                                                       <  2:2927386/148‑1 (MQ=255)
          aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGACCTAAAAAATATATTCAATCGTATTAAATAAAAATAtt                                                                                                                                      >  1:3226418/1‑148 (MQ=255)
          aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAtt                                                                                                                                      <  1:2679559/148‑1 (MQ=255)
          aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAtt                                                                                                                                      >  1:3226603/1‑148 (MQ=255)
            aaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTc                                                                                                                                    >  1:1306534/1‑148 (MQ=255)
             aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCg                                                                                                                                   <  1:713084/148‑1 (MQ=255)
             aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   <  1:179418/148‑1 (MQ=255)
              aGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGt                                                                                                                                  >  1:1039790/1‑148 (MQ=255)
               gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 >  1:2956537/1‑148 (MQ=255)
               gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 <  2:2356706/148‑1 (MQ=255)
               gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 >  1:2206005/1‑148 (MQ=255)
                cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGa                                                                                                                                <  2:2381972/148‑1 (MQ=255)
                cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGa                                                                                                                                <  2:16907/148‑1 (MQ=255)
                cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGa                                                                                                                                >  2:803020/1‑148 (MQ=255)
                  tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGt                                                                                                                              <  1:2440099/148‑1 (MQ=255)
                   cTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtc                                                                                                                             >  1:1978003/1‑148 (MQ=255)
                    tGCGACAGGGCAAATCTCGGTATATGATTTGCGCACGGTATTACCTATTTTACACCAGGCATAAGCAATAATATGTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtct                                                                                                                            <  2:1755159/148‑1 (MQ=255)
                     gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGtctc                                                                                                                           >  2:1068581/1‑148 (MQ=255)
                     gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                           <  2:123072/148‑1 (MQ=255)
                     gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                           <  2:123207/148‑1 (MQ=255)
                      cgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTctct                                                                                                                          <  1:57855/148‑1 (MQ=255)
                           gggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     <  1:1522978/148‑1 (MQ=255)
                            ggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                    >  2:291449/1‑148 (MQ=255)
                             gCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCt                                                                                                                   >  1:2527628/1‑148 (MQ=255)
                                aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                                <  2:1124154/148‑1 (MQ=255)
                                 aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               >  1:1101549/1‑148 (MQ=255)
                                   ctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtct                                                                                                             >  2:2687525/1‑148 (MQ=255)
                                   ctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtct                                                                                                             >  1:179980/1‑148 (MQ=255)
                                     cGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                           >  1:1820004/1‑148 (MQ=255)
                                        tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAtt                                                                                                        <  1:1918615/148‑1 (MQ=255)
                                        tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAtt                                                                                                        >  2:74084/1‑148 (MQ=255)
                                        tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAtt                                                                                                        <  1:2377903/148‑1 (MQ=255)
                                           aTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTgg                                                                                                     >  1:3255923/1‑148 (MQ=255)
                                            tGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGc                                                                                                    >  1:2369695/1‑148 (MQ=255)
                                                ttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                >  2:320756/1‑148 (MQ=255)
                                                ttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                >  2:2470704/1‑148 (MQ=255)
                                                   cgcACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGa                                                                                             >  1:2573386/1‑148 (MQ=255)
                                                        ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATc                                                                                        <  1:316594/148‑1 (MQ=255)
                                                           aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcaca                                                                                     <  1:2744383/148‑1 (MQ=255)
                                                           aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcaca                                                                                     >  2:2709053/1‑148 (MQ=255)
                                                           aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcaca                                                                                     <  1:84364/148‑1 (MQ=255)
                                                            ttAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCTTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt                                                                                    <  2:1897/148‑1 (MQ=255)
                                                            ttAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt                                                                                    <  2:2646835/148‑1 (MQ=255)
                                                            ttAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt                                                                                    >  1:1013208/1‑148 (MQ=255)
                                                             tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                   <  1:142926/148‑1 (MQ=255)
                                                             tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                   >  1:3022662/1‑148 (MQ=255)
                                                               aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTg                                                                                 >  2:2578716/1‑148 (MQ=255)
                                                               aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTg                                                                                 >  2:1804501/1‑148 (MQ=255)
                                                                cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                >  1:809585/1‑148 (MQ=255)
                                                                 tATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa                                                                               <  1:1391480/148‑1 (MQ=255)
                                                                         acCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAt                                                                       <  2:680871/148‑1 (MQ=255)
                                                                           cAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTa                                                                     >  1:1538455/1‑148 (MQ=255)
                                                                               cATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACaa                                                                 <  1:391008/148‑1 (MQ=255)
                                                                                  aaGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                              >  1:1675076/1‑148 (MQ=255)
                                                                                  aaGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                              >  1:2151134/1‑148 (MQ=255)
                                                                                   aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaa                                                             >  1:2173195/1‑148 (MQ=255)
                                                                                    gCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaaa                                                            >  1:450748/1‑148 (MQ=255)
                                                                                       ataataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCt                                                         >  1:2294214/1‑148 (MQ=255)
                                                                                        taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAACATCACATCTGAATCAGGAATTAACAATGAAACCTg                                                        >  1:1734186/1‑148 (MQ=255)
                                                                                        taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTg                                                        >  2:667032/1‑148 (MQ=255)
                                                                                         aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                       >  1:63683/1‑148 (MQ=255)
                                                                                           tatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaa                                                     >  2:469562/1‑148 (MQ=255)
                                                                                            atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                    >  2:887843/1‑148 (MQ=255)
                                                                                            atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                    >  1:2046221/1‑148 (MQ=255)
                                                                                              ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                  >  2:473167/1‑148 (MQ=255)
                                                                                                cggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacca                                                <  2:1575992/148‑1 (MQ=255)
                                                                                                      gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                          >  2:2835751/1‑148 (MQ=255)
                                                                                                       aaCACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCgga                                         >  1:2410629/1‑146 (MQ=255)
                                                                                                       aaCACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                         <  1:238934/148‑1 (MQ=255)
                                                                                                         cacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAtt                                       >  2:3166570/1‑148 (MQ=255)
                                                                                                          acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                      <  1:1182829/148‑1 (MQ=255)
                                                                                                          acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                      <  2:2521900/148‑1 (MQ=255)
                                                                                                            ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAACATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                    >  2:2016363/1‑148 (MQ=255)
                                                                                                            ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                    >  1:2864707/1‑148 (MQ=255)
                                                                                                             cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATg                                   >  2:1335028/1‑148 (MQ=255)
                                                                                                                cccGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCA‑CTCGTATTAATGGACg                               >  2:1429464/1‑148 (MQ=255)
                                                                                                                  cgccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAACGAAACCTGTAAAACCACCTCGTATTAATGGACGa                              <  1:3089122/148‑1 (MQ=255)
                                                                                                                   gccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAg                             <  2:340306/148‑1 (MQ=255)
                                                                                                                        aCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCg                        <  1:2470704/148‑1 (MQ=255)
                                                                                                                             aaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCt                   >  1:3232056/1‑148 (MQ=255)
                                                                                                                                atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                >  1:882354/1‑148 (MQ=255)
                                                                                                                                atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                >  2:1962238/1‑148 (MQ=255)
                                                                                                                                 tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg               >  1:1143397/1‑148 (MQ=255)
                                                                                                                                         aTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa       >  2:1596943/1‑148 (MQ=255)
                                                                                                                                         aTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa       <  2:1869659/148‑1 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      >  1:559944/1‑148 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      <  2:1306534/148‑1 (MQ=255)
                                                                                                                                           cGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg     >  1:516409/1‑148 (MQ=255)
                                                                                                                                           cGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg     <  2:428557/148‑1 (MQ=255)
                                                                                                                                             tATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   >  2:1001595/1‑148 (MQ=255)
                                                                                                                                              aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  <  2:591508/148‑1 (MQ=255)
                                                                                                                                              aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  <  1:341299/148‑1 (MQ=255)
                                                                                                                                              aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  <  1:2007499/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769618‑1769907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑