Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,803,109 C→T 90.4% A128T (GCC→ACC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,803,1090CT90.4% 625.6 / 48.1 208A128T (GCC→ACC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (11/9);  new base T (107/81);  total (118/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 216 total aligned reads displayed.

CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATT  >  CP009273/1802964‑1803256
                                                                                                                                                 |                                                                                                                                                   
ccAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGttt                                                                                                                                                   <  2:2617143/148‑1 (MQ=255)
ccAAGCAGAGAAAAGAACACAGAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGttt                                                                                                                                                   <  2:1970925/148‑1 (MQ=255)
 cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGtttt                                                                                                                                                  >  1:1295763/1‑148 (MQ=255)
    gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTc                                                                                                                                               <  1:2840039/148‑1 (MQ=255)
     cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTcc                                                                                                                                              <  2:2662372/148‑1 (MQ=255)
     cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTcc                                                                                                                                              <  2:2945590/148‑1 (MQ=255)
         gaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGaa                                                                                                                                          >  1:1367508/1‑148 (MQ=255)
           aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATg                                                                                                                                        >  2:2867882/1‑148 (MQ=255)
               aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                    <  2:1058032/148‑1 (MQ=255)
                acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg                                                                                                                                   >  2:1004721/1‑148 (MQ=255)
                 cacaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGa                                                                                                                                  <  1:323851/148‑1 (MQ=255)
                   caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATc                                                                                                                                <  2:649399/148‑1 (MQ=255)
                     tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCtt                                                                                                                              >  2:1367841/1‑148 (MQ=255)
                       aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATCCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCa                                                                                                                            <  2:3163874/148‑1 (MQ=255)
                       aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCa                                                                                                                            >  1:2124116/1‑148 (MQ=255)
                        ccTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                           >  2:3145122/1‑148 (MQ=255)
                          tGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAaca                                                                                                                         <  1:1626180/148‑1 (MQ=255)
                           gcagcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                        <  2:1358344/148‑1 (MQ=255)
                            cagcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACAcc                                                                                                                       <  1:2989163/148‑1 (MQ=255)
                                aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc                                                                                                                   <  2:3077544/148‑1 (MQ=255)
                                  tACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCg                                                                                                                 >  2:1205857/1‑148 (MQ=255)
                                   aCAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc                                                                                                                >  1:2489280/1‑148 (MQ=255)
                                    cAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCa                                                                                                               >  1:2110772/1‑148 (MQ=255)
                                     aaaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaa                                                                                                              >  2:85559/1‑148 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                            <  2:2464112/148‑1 (MQ=255)
                                         aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaag                                                                                                          <  1:2036263/148‑1 (MQ=255)
                                         aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaag                                                                                                          <  1:2561715/148‑1 (MQ=255)
                                           ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                        <  2:1295763/148‑1 (MQ=255)
                                           ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                        <  2:2185887/148‑1 (MQ=255)
                                           ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                        >  2:635117/1‑148 (MQ=255)
                                              gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc                                                                                                     >  1:1594652/1‑148 (MQ=255)
                                               cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                    >  2:3191266/1‑148 (MQ=255)
                                                   atatACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                                                                                >  2:1773179/1‑148 (MQ=255)
                                                   atatACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                                                                                >  1:1250698/1‑148 (MQ=255)
                                                    tataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCg                                                                                               >  1:2147409/1‑148 (MQ=255)
                                                          gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt                                                                                         >  1:1935488/1‑148 (MQ=255)
                                                            gagaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATcc                                                                                       >  1:1933228/1‑148 (MQ=255)
                                                             agaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                                      <  2:2984082/148‑1 (MQ=255)
                                                               aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGATAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACATTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg                                                                                    >  1:1582261/1‑148 (MQ=255)
                                                                aaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGc                                                                                   >  2:1230179/1‑148 (MQ=255)
                                                                 aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                  >  1:852003/1‑148 (MQ=255)
                                                                 aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                  >  2:2048644/1‑148 (MQ=255)
                                                                 aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                                  >  1:671564/1‑148 (MQ=255)
                                                                  aaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAc                                                                                 >  2:2214650/1‑148 (MQ=255)
                                                                   aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCACGAAGCGCGCCTGGCGACTCATCCGGGCAcc                                                                                >  2:2897773/1‑148 (MQ=255)
                                                                   aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAcc                                                                                >  2:1574207/1‑148 (MQ=255)
                                                                       cAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGac                                                                            >  1:2243083/1‑147 (MQ=255)
                                                                       cAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGaa                                                                            >  1:1663328/1‑148 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                          <  1:2947310/148‑1 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                          <  1:184198/148‑1 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                          <  1:208529/148‑1 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                          <  1:1742011/148‑1 (MQ=255)
                                                                            aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTg                                                                       >  1:2375228/1‑148 (MQ=255)
                                                                            aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTg                                                                       >  2:2336160/1‑148 (MQ=255)
                                                                             aaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGc                                                                      >  2:1603315/1‑148 (MQ=255)
                                                                              aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCa                                                                     >  2:814877/1‑148 (MQ=255)
                                                                                aGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc                                                                   >  2:1674840/1‑148 (MQ=255)
                                                                                  tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGc                                                                 >  1:1887194/1‑148 (MQ=255)
                                                                                    tGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa                                                               >  2:372238/1‑148 (MQ=255)
                                                                                     ggTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAg                                                              >  2:3268766/1‑148 (MQ=255)
                                                                                      gTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGt                                                             <  1:257359/148‑1 (MQ=255)
                                                                                       tGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTc                                                            <  1:580247/148‑1 (MQ=255)
                                                                                           gAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGAc                                                        >  1:230542/1‑148 (MQ=255)
                                                                                           gAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCATCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGAc                                                        >  2:1075749/1‑148 (MQ=255)
                                                                                               aacaaTCCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaa                                                    <  2:942473/148‑1 (MQ=255)
                                                                                               aacaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTACCTCAAGAAGCGCGCCTGTCGACTCATCCGGGCACCGGAATCCTGCGACTCAAGTCCGACaaaa                                                    <  2:3176807/148‑1 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                   >  1:2170214/1‑148 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                   <  2:366568/148‑1 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                   <  1:1004721/148‑1 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCGTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                   <  2:2155323/148‑1 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCCCCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                                  >  1:726406/1‑148 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                                  >  1:816117/1‑148 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                                  >  1:1190077/1‑148 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                                  <  1:244247/148‑1 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCACGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                              >  2:3266799/1‑148 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                              <  1:2228374/148‑1 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                              <  1:3182904/148‑1 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                              >  2:3261706/1‑148 (MQ=255)
                                                                                                           cTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaa                                        <  1:2402185/148‑1 (MQ=255)
                                                                                                           cTCAATTTGTTCACGCGTTAGCCCCTCGTCCCCTTGGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaa                                        <  2:2366476/148‑1 (MQ=255)
                                                                                                             cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                      <  1:191831/148‑1 (MQ=255)
                                                                                                             cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt                                      <  2:406807/148‑1 (MQ=255)
                                                                                                                tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAc                                   >  1:1941340/1‑148 (MQ=255)
                                                                                                                       aCGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAAc                            >  1:3261065/1‑148 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                         >  1:170202/1‑148 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                         >  1:570196/1‑148 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                         <  1:3198627/148‑1 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                         <  1:1132781/148‑1 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                      >  2:668510/1‑148 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                      >  2:1441107/1‑148 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                      >  2:1441185/1‑148 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc                   <  1:85559/148‑1 (MQ=255)
                                                                                                                                   ctctTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACg                >  1:3195517/1‑148 (MQ=255)
                                                                                                                                      ttCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca             <  1:814877/148‑1 (MQ=255)
                                                                                                                                       tCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac            >  2:3198462/1‑148 (MQ=255)
                                                                                                                                       tCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac            >  1:596104/1‑148 (MQ=255)
                                                                                                                                       tCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACACAGAACATAACGTTCACGccac            >  2:3198751/1‑148 (MQ=255)
                                                                                                                                           cTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt        >  1:377988/1‑148 (MQ=255)
                                                                                                                                             ttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaa      >  1:2822198/1‑148 (MQ=255)
                                                                                                                                              tGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa     >  1:1453524/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATT  >  CP009273/1802964‑1803256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑