BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,836,192	T→A	90.7%	I67I (ATA→ATT)	ynjH ←	DUF1496 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,836,192	0	T	A	90.7%	380.1 / 24.0	128	I67I (ATA→ATT)	ynjH	DUF1496 family protein
Reads supporting (aligned to +/- strand): ref base T (2/10); new base A (50/66); total (52/76)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGT  >  CP009273/1836050‑1836337

gtatggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTTACATTCAATTATTAGCGGCGTTTATGTCTTTACGCGCCGACAGACCAGCGGATTAGTGCTAAGGGTTTTGTCTTCACGCTGGCATTGCAGCAGAATTcc                                                                                                                                              <  2:1679305/145‑1 (MQ=255)

 tctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                             <  1:2589870/148‑1 (MQ=255)

 tctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                             <  1:885027/148‑1 (MQ=255)

 tctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATTGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTGATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                             >  1:110277/1‑148 (MQ=255)

     gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                         >  2:2632633/1‑148 (MQ=255)

     gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                         <  1:658536/148‑1 (MQ=255)

       gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                       <  2:1450750/148‑1 (MQ=255)

        cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCtt                                                                                                                                      <  1:690357/148‑1 (MQ=255)

         tCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTGGCTTTAAGCGCCGCCCCACCAGCGGCTTAGTGCTAAGGGGTTTGTGATCACGCAGGCAATGGAGCAGAAATCCTTCGGCttt                                                                                                                                     >  1:3147649/1‑148 (MQ=255)

          cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                                    <  1:1580933/148‑1 (MQ=255)

          cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                                    <  1:2852380/148‑1 (MQ=255)

          cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                                    <  1:2054808/148‑1 (MQ=255)

           aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                   >  2:2100861/1‑148 (MQ=255)

           aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                   >  2:1653686/1‑148 (MQ=255)

           aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                   <  1:1085375/148‑1 (MQ=255)

            ccTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAt                                                                                                                                  <  2:1944871/148‑1 (MQ=255)

              tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCCGACAAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTACGTCGGCGTTAATTa                                                                                                                                >  2:3119851/1‑148 (MQ=255)

              tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTa                                                                                                                                <  1:1975108/148‑1 (MQ=255)

               gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTCCAGCAGAATTCCTTCGGCTTTAATTAc                                                                                                                               <  1:1805089/148‑1 (MQ=255)

                 agagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCg                                                                                                                             <  1:966573/148‑1 (MQ=255)

                  gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGc                                                                                                                            <  2:2848725/148‑1 (MQ=255)

                  gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGc                                                                                                                            <  2:2850634/148‑1 (MQ=255)

                    ggCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGccc                                                                                                                          <  2:3049581/148‑1 (MQ=255)

                    ggCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGGGTTTTGGTTTAACCGGCGCCAGACCAGCGGCTTAGTGGAAAGGGGGTTGTGATCACGCGTGCCTTTCAGCAGAATTACTTCGGGTTTAATTaaacccc                                                                                                                          >  1:2659327/1‑142 (MQ=255)

                     gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATGGCGTTTCGCGCCGCCAGACCAGCGGCTTAGTGGTAAGGGGTTTGTGATCACGCGGGGATTGCAGCAGTATTGCTTCGGGGTTTATTAAcccccc                                                                                                                         >  1:1185402/1‑148 (MQ=255)

                        cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGAGTAGTGCTAAGGGTTTTGTCATCACGCTGGCCTTGCACCAGTATTCCTTCGGCTTTAATTACCGCCCCTTc                                                                                                                      >  2:2926308/1‑148 (MQ=255)

                          gCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTTTGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACGCCCCTTccg                                                                                                                    >  2:1446634/1‑146 (MQ=255)

                           cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCGTTAATTACCGCCCCTTCAGa                                                                                                                   >  2:797218/1‑148 (MQ=255)

                            aaTATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                                                                                  <  2:660245/148‑1 (MQ=255)

                              tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaata                                                                                                                <  2:813899/148‑1 (MQ=255)

                               atCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTAGGCTTTAATTACCGCCCCGTCAGaaaaa                                                                                                               >  1:1623646/1‑148 (MQ=255)

                                tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTGGCTTTTCGCGCCGCCAGACCAGCGGATTAGTGGTAAGGGTTTTGTCATAACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTTCCGCCCCGTCAGaataat                                                                                                              >  2:397718/1‑148 (MQ=255)

                                 ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtt                                                                                                             <  1:2541499/148‑1 (MQ=255)

                                 ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtt                                                                                                             <  1:1805355/148‑1 (MQ=255)

                                 ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtt                                                                                                             <  2:2995658/148‑1 (MQ=255)

                                 ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtt                                                                                                             <  1:386647/148‑1 (MQ=255)

                                  cGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAttt                                                                                                            <  2:1796324/148‑1 (MQ=255)

                                     tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                                                                         <  2:2465586/148‑1 (MQ=255)

                                       gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTTAta                                                                                                       >  2:3265468/1‑147 (MQ=255)

                                       gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                                                                       <  1:1815737/148‑1 (MQ=255)

                                       gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                                                                       <  2:3206873/148‑1 (MQ=255)

                                       gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCGTTACGCGCCGCCACACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCGGCAGAATTACTTCGGCTTTAATTACCGCCCCAACAGAATAATTTTGATc                                                                                                       >  1:2943369/1‑148 (MQ=255)

                                          ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                                    <  2:2627123/148‑1 (MQ=255)

                                          ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCACACCAGCGGATTAGTGCTAAGGGTTTTGTGATCACGCGGGCATTGCAGCAGAAATACTTCGGCTTTAATTACCCCCCCTTCAGAAGAAATTTGATCCTg                                                                                                    >  1:2907283/1‑148 (MQ=255)

                                              ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                                <  2:73643/148‑1 (MQ=255)

                                                 aCATTCCATTATTAGAGGCGTTTAAGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGGTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTGCGGGGTTAATTACCGCCCCGTCAGCATAAATTGGTTCGTGCTTAACg                                                                                             >  2:2257222/1‑148 (MQ=255)

                                                  cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                            <  1:3291975/148‑1 (MQ=255)

                                                  cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                            <  1:1904548/148‑1 (MQ=255)

                                                  cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                            <  1:2371980/148‑1 (MQ=255)

                                                           attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAg                                                                                   <  1:2100861/148‑1 (MQ=255)

                                                           attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCGTTAATTACCGCCCCATCAGAATAATTTTGATCCTGATAAAAGCAGCACagag                                                                                   >  2:1927251/1‑148 (MQ=255)

                                                               gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                               <  1:286271/148‑1 (MQ=255)

                                                                 ggCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAgg                                                                             <  1:2109905/148‑1 (MQ=255)

                                                                  gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                            <  2:909333/148‑1 (MQ=255)

                                                                  gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                            <  2:1670115/148‑1 (MQ=255)

                                                                   cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGc                                                                           <  2:1067874/148‑1 (MQ=255)

                                                                   cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGc                                                                           <  2:2719518/148‑1 (MQ=255)

                                                                      ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                                        >  1:2485941/1‑148 (MQ=255)

                                                                       tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgcg                                                                       <  2:2781330/148‑1 (MQ=255)

                                                                           gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTATGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTAAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                   <  1:18919/148‑1 (MQ=255)

                                                                           gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                   <  2:1428382/148‑1 (MQ=255)

                                                                           gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                   <  2:1420146/148‑1 (MQ=255)

                                                                           gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                   >  2:197911/1‑148 (MQ=255)

                                                                           gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                   <  2:1420148/148‑1 (MQ=255)

                                                                            cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                  >  1:2140795/1‑148 (MQ=255)

                                                                              ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                                >  1:1203803/1‑148 (MQ=255)

                                                                              ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                                >  1:1410102/1‑148 (MQ=255)

                                                                               tACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGc                                                               <  2:2668870/148‑1 (MQ=255)

                                                                                          aGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCATCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaact                                                    >  1:1782777/1‑148 (MQ=255)

                                                                                           gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactg                                                   >  1:505254/1‑148 (MQ=255)

                                                                                            aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCATGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactga                                                  >  2:729570/1‑148 (MQ=255)

                                                                                              cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                                <  2:2334792/148‑1 (MQ=255)

                                                                                               aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAaca                                               <  2:2039082/148‑1 (MQ=255)

                                                                                               aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAaca                                               <  2:2039014/148‑1 (MQ=255)

                                                                                                     ttAGTGATAAGGTTTTTGTCATCACGCTGGCATTGCAGCATAATACCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTg                                         <  1:2772452/148‑1 (MQ=255)

                                                                                                      tAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                                        <  2:1768578/148‑1 (MQ=255)

                                                                                                          gCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                                    >  2:408684/1‑148 (MQ=255)

                                                                                                          gCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                                    >  2:280369/1‑148 (MQ=255)

                                                                                                          gCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                                    >  1:2357938/1‑148 (MQ=255)

                                                                                                                ggTTTTGTCATCACGCTGGCATTGCAGCAGAATACCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGTCGGTACGttt                              >  2:2852383/1‑148 (MQ=255)

                                                                                                                 gTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTa                             >  1:2011295/1‑148 (MQ=255)

                                                                                                                  ttttGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                            <  1:513996/148‑1 (MQ=255)

                                                                                                                      gTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                        <  1:2845536/148‑1 (MQ=255)

                                                                                                                         atcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGGACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACg                     >  2:2873245/1‑148 (MQ=255)

                                                                                                                          tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTCCGTTTACCTCCACGt                    >  1:1650997/1‑148 (MQ=255)

                                                                                                                          tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                    >  1:1911728/1‑148 (MQ=255)

                                                                                                                          tcaCGCTGGCAGTGCAGCATAATTCCTTCGGCTTTAATTACCCCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGACCTGAACACTTCTGGCGGTACGTTTACCTCCACGt                    <  1:3087223/148‑1 (MQ=255)

                                                                                                                           caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                   >  1:3018529/1‑148 (MQ=255)

                                                                                                                           caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                   >  1:2482233/1‑148 (MQ=255)

                                                                                                                           caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                   >  1:2214302/1‑148 (MQ=255)

                                                                                                                              gCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCgg                <  2:1447984/148‑1 (MQ=255)

                                                                                                                              gCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCgg                >  2:335667/1‑148 (MQ=255)

                                                                                                                                  gCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGa            >  1:192314/1‑148 (MQ=255)

                                                                                                                                     ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa         >  1:2350177/1‑148 (MQ=255)

                                                                                                                                     ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa         >  1:2350166/1‑148 (MQ=255)

                                                                                                                                       gcagcaGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATg       <  2:924648/148‑1 (MQ=255)

                                                                                                                                       gcagcaGAATTCCTTCGGATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATg       <  1:2087745/148‑1 (MQ=255)

                                                                                                                                         agcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCg     <  2:631023/148‑1 (MQ=255)

                                                                                                                                          gcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCgg    <  2:838826/148‑1 (MQ=255)

                                                                                                                                            agTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGt  <  1:2181863/148‑1 (MQ=255)

GTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGT  >  CP009273/1836050‑1836337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`