Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,842,560 T→A 12.2% Q125L (CAG→CTG)  ydjA ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,842,5600TA12.2% 627.4 / 64.6 221Q125L (CAG→CTG) ydjAputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (90/103);  new base A (9/18);  total (100/121)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

GGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCG  >  CP009273/1842414‑1842706
                                                                                                                                                  |                                                                                                                                                  
ggACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTg                                                                                                                                                   <  1:165666/148‑1 (MQ=255)
   cGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCCTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGc                                                                                                                                                >  1:990934/1‑148 (MQ=255)
      tAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAAc                                                                                                                                             >  1:2988849/1‑148 (MQ=255)
        aTCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAActg                                                                                                                                           >  1:1834736/1‑148 (MQ=255)
        aTCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAActg                                                                                                                                           <  1:896032/148‑1 (MQ=255)
            tcGTTGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACtgctgc                                                                                                                                       <  2:272612/147‑1 (MQ=255)
            aCGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACtgctgc                                                                                                                                       <  1:510967/148‑1 (MQ=255)
               tAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCAt                                                                                                                                    >  2:642799/1‑148 (MQ=255)
               tAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCAt                                                                                                                                    >  1:295661/1‑148 (MQ=255)
                aGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCAtt                                                                                                                                   >  1:1561187/1‑148 (MQ=255)
                  aTGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTg                                                                                                                                 >  2:456005/1‑148 (MQ=255)
                  aTGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTg                                                                                                                                 >  1:442246/1‑148 (MQ=255)
                   tGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCCAACCCCTGGGCAACTGCTGCCATTTGc                                                                                                                                >  2:1628977/1‑148 (MQ=255)
                   tGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGc                                                                                                                                <  2:776691/148‑1 (MQ=255)
                      tttCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATc                                                                                                                             >  1:750206/1‑148 (MQ=255)
                        tCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGc                                                                                                                           <  1:3048106/148‑1 (MQ=255)
                          aGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCa                                                                                                                         >  2:2721414/1‑148 (MQ=255)
                            cTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATg                                                                                                                       <  2:2041162/148‑1 (MQ=255)
                                  cgTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACcgcg                                                                                                                 <  2:1291837/148‑1 (MQ=255)
                                  cgTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACcgcg                                                                                                                 >  2:198947/1‑148 (MQ=255)
                                     aCCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCAt                                                                                                              <  2:2644363/148‑1 (MQ=255)
                                          ggTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGc                                                                                                         >  1:2592946/1‑148 (MQ=255)
                                          ggTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGc                                                                                                         <  1:1625150/148‑1 (MQ=255)
                                           gTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCa                                                                                                        >  2:2776436/1‑148 (MQ=255)
                                             agagAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGa                                                                                                      >  1:2283656/1‑148 (MQ=255)
                                             agagAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGa                                                                                                      <  1:873319/148‑1 (MQ=255)
                                              gagaAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGAc                                                                                                     >  2:2518044/1‑148 (MQ=255)
                                               agaAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACa                                                                                                    >  2:72227/1‑148 (MQ=255)
                                                 aaaaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAtt                                                                                                  <  1:2899528/148‑1 (MQ=255)
                                                  aaaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAttt                                                                                                 <  2:1568690/148‑1 (MQ=255)
                                                  aaaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAttt                                                                                                 >  2:1447046/1‑148 (MQ=255)
                                                  aaaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAttt                                                                                                 >  1:1368476/1‑148 (MQ=255)
                                                   aaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTc                                                                                                >  1:3248483/1‑148 (MQ=255)
                                                   aaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTc                                                                                                >  1:2533768/1‑148 (MQ=255)
                                                      cGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTg                                                                                             <  1:671802/148‑1 (MQ=255)
                                                        aCAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGtt                                                                                           >  1:2910117/1‑148 (MQ=255)
                                                        aCAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGtt                                                                                           >  2:3284213/1‑148 (MQ=255)
                                                         cAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTc                                                                                          <  2:2309354/148‑1 (MQ=255)
                                                          aaTTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTcc                                                                                         <  1:2769644/148‑1 (MQ=255)
                                                          aaTTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTcc                                                                                         <  1:653491/148‑1 (MQ=255)
                                                           aTTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTccc                                                                                        >  2:1823785/1‑148 (MQ=255)
                                                             tttATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAg                                                                                      >  2:3229003/1‑148 (MQ=255)
                                                                aaccTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTGGCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATGTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGATCCCAgcgc                                                                                   >  2:2132358/3‑148 (MQ=255)
                                                                       tCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACtt                                                                            >  1:1005741/1‑148 (MQ=255)
                                                                        cACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACttt                                                                           <  2:1286241/148‑1 (MQ=255)
                                                                        cACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACttt                                                                           <  2:1736870/148‑1 (MQ=255)
                                                                        cACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACttt                                                                           >  1:1898859/1‑148 (MQ=255)
                                                                         aCGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTa                                                                          <  2:2934105/148‑1 (MQ=255)
                                                                           ggCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATg                                                                        <  2:3142810/148‑1 (MQ=255)
                                                                             cAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACCTTTCCTGTTCCCAGCGCGGGACTTTATGAt                                                                      >  2:2149890/1‑148 (MQ=255)
                                                                              aaCCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGAtt                                                                     <  2:2236424/148‑1 (MQ=255)
                                                                                  gAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTctct                                                                 <  1:2076070/148‑1 (MQ=255)
                                                                                   aaTGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCtt                                                                <  1:964159/148‑1 (MQ=255)
                                                                                      gCTTCACGCACTACCGGACTTTCAGTAAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCAAGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGc                                                             >  2:1223458/1‑148 (MQ=255)
                                                                                       cTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCa                                                            >  2:1661987/1‑148 (MQ=255)
                                                                                        ttCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCAt                                                           <  2:241759/148‑1 (MQ=255)
                                                                                         tCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCAtt                                                          >  1:1756746/1‑148 (MQ=255)
                                                                                          cACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCAttt                                                         <  2:2750129/148‑1 (MQ=255)
                                                                                            cGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCg                                                       >  1:1028635/1‑148 (MQ=255)
                                                                                            cGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCg                                                       <  2:3262238/148‑1 (MQ=255)
                                                                                            cGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCg                                                       >  1:2735796/1‑148 (MQ=255)
                                                                                                cTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGccac                                                   >  2:1516403/1‑148 (MQ=255)
                                                                                                  aCCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGccacca                                                 >  1:937641/1‑148 (MQ=255)
                                                                                                   ccGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCcaccac                                                >  2:41597/1‑148 (MQ=255)
                                                                                                    cGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCaccacc                                               >  2:1868742/1‑148 (MQ=255)
                                                                                                        cTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtga                                           <  1:2126910/148‑1 (MQ=255)
                                                                                                           tCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatga                                        <  2:2592946/148‑1 (MQ=255)
                                                                                                           tCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatga                                        <  1:2295143/148‑1 (MQ=255)
                                                                                                            cAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatgat                                       <  2:204789/148‑1 (MQ=255)
                                                                                                            cAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatgat                                       <  1:2383186/148‑1 (MQ=255)
                                                                                                              gTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatgatga                                     <  1:2996725/148‑1 (MQ=255)
                                                                                                              gTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatgatga                                     <  2:2200761/148‑1 (MQ=255)
                                                                                                               ttAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAg                                    <  1:2002440/148‑1 (MQ=255)
                                                                                                                 aaTGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCg                                  >  1:2676811/1‑148 (MQ=255)
                                                                                                                 aaTGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCg                                  >  1:1107800/1‑148 (MQ=255)
                                                                                                                 aaTGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCg                                  <  1:2893114/148‑1 (MQ=255)
                                                                                                                   tGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGt                                >  2:701651/1‑148 (MQ=255)
                                                                                                                   tGCGCCACTGCGCCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGt                                <  2:2311850/148‑1 (MQ=255)
                                                                                                                         aCTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCgg                          <  1:2282137/148‑1 (MQ=255)
                                                                                                                          cTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGa                         <  1:1081390/148‑1 (MQ=255)
                                                                                                                           tGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGaa                        <  2:2144593/148‑1 (MQ=255)
                                                                                                                           tGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGaa                        <  1:320013/148‑1 (MQ=255)
                                                                                                                            gcgcCAGATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAAc                       <  2:1784549/148‑1 (MQ=255)
                                                                                                                             cgcCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACg                      <  2:442246/148‑1 (MQ=255)
                                                                                                                                 aGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGgcgc                  >  1:201953/1‑148 (MQ=255)
                                                                                                                                  gATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCa                 >  1:2633444/1‑148 (MQ=255)
                                                                                                                                  gATGCCGCCAAACCCCAGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCa                 <  2:1168291/148‑1 (MQ=255)
                                                                                                                                    tGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCAtt               <  1:1447046/148‑1 (MQ=255)
                                                                                                                                     gccgccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTa              <  1:198947/148‑1 (MQ=255)
                                                                                                                                         ccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACggg          <  1:1832976/148‑1 (MQ=255)
                                                                                                                                         ccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACggg          >  2:316654/1‑148 (MQ=255)
                                                                                                                                           aaaCCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCt        >  2:1100264/1‑148 (MQ=255)
                                                                                                                                            aaCCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCtt       >  1:1069374/1‑148 (MQ=255)
                                                                                                                                             aCCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCttt      <  1:3139902/148‑1 (MQ=255)
                                                                                                                                              ccccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTg     >  2:2336158/1‑148 (MQ=255)
                                                                                                                                                ccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTc   >  2:2965902/1‑148 (MQ=255)
                                                                                                                                                ccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTc   >  1:644217/1‑148 (MQ=255)
                                                                                                                                                ccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTc   >  2:2433387/1‑148 (MQ=255)
                                                                                                                                                 cTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCg  >  1:173674/1‑148 (MQ=255)
                                                                                                                                                 cTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCg  <  1:2776436/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCG  >  CP009273/1842414‑1842706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑