Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,850,880 C→A 11.2% V102V (GTG→GTT)  ydjH ← putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,850,8800CA11.2% 724.7 / 45.5 213V102V (GTG→GTT) ydjHputative kinase
Reads supporting (aligned to +/- strand):  ref base C (90/99);  new base A (12/12);  total (102/111)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 215 total aligned reads displayed.

ACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGA  >  CP009273/1850736‑1851027
                                                                                                                                                |                                                                                                                                                   
 ccATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCATTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCa                                                                                                                                                 <  1:2834833/148‑1 (MQ=255)
    tCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTc                                                                                                                                              <  1:365332/148‑1 (MQ=255)
      cAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCa                                                                                                                                            <  2:1797441/148‑1 (MQ=255)
       aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCaa                                                                                                                                           >  1:1213789/1‑148 (MQ=255)
       aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCaa                                                                                                                                           <  2:2571274/148‑1 (MQ=255)
         tAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                                                                                                         >  1:1098042/1‑148 (MQ=255)
            tGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTa                                                                                                                                      >  1:85202/1‑148 (MQ=255)
            tGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTa                                                                                                                                      <  2:1922873/148‑1 (MQ=255)
            tGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTa                                                                                                                                      <  2:653266/148‑1 (MQ=255)
            tGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTa                                                                                                                                      <  2:653233/148‑1 (MQ=255)
               aCTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCAACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATa                                                                                                                                   >  2:1953556/1‑148 (MQ=255)
                 tGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATaga                                                                                                                                 <  2:1945346/148‑1 (MQ=255)
                    tGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGt                                                                                                                              >  2:2925272/1‑148 (MQ=255)
                     gAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTa                                                                                                                             <  2:1569388/148‑1 (MQ=255)
                     gAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTa                                                                                                                             <  1:2115376/148‑1 (MQ=255)
                       aaaTACTGGCCTGTTATAATAATTTCGCCTGAGAAAACCGCGAAAATTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATc                                                                                                                           <  1:3128381/148‑1 (MQ=255)
                       aaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATc                                                                                                                           <  2:2106953/148‑1 (MQ=255)
                           aCTGGCCAGTTATAATAATTTCGCCTGATAAAACCGCCCAAAATCAACATCGACAATATTAAATTTCCACAGACTGCCATTACGTTTTGTGACACACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATg                                                                                                                       <  1:1414901/148‑1 (MQ=255)
                           aCTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATg                                                                                                                       <  2:1195390/148‑1 (MQ=255)
                           aCTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATg                                                                                                                       >  1:1415470/1‑148 (MQ=255)
                                  aGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGt                                                                                                                >  1:1935827/1‑148 (MQ=255)
                                   gTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTc                                                                                                               >  1:85436/1‑148 (MQ=255)
                                      ataataATTTCGCCTGATAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTg                                                                                                            <  1:1964778/148‑1 (MQ=255)
                                      ataataATTTCGCCTGATAAAACCGCGAAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTg                                                                                                            <  1:2499961/148‑1 (MQ=255)
                                       taataaTTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGc                                                                                                           <  2:2903176/148‑1 (MQ=255)
                                         ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCtt                                                                                                         <  1:2857659/148‑1 (MQ=255)
                                         ataatTGCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCtt                                                                                                         <  2:2790766/148‑1 (MQ=255)
                                          taatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTc                                                                                                        >  1:1685938/1‑148 (MQ=255)
                                             tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAgg                                                                                                     <  2:1633379/148‑1 (MQ=255)
                                             tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAgg                                                                                                     <  1:38194/148‑1 (MQ=255)
                                              ttCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAATCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGc                                                                                                    >  1:733472/1‑148 (MQ=255)
                                               tCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCt                                                                                                   <  2:1272754/148‑1 (MQ=255)
                                                cGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCtt                                                                                                  <  2:913826/148‑1 (MQ=255)
                                                  ccTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTg                                                                                                >  2:2664910/1‑148 (MQ=255)
                                                   cTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGa                                                                                               <  1:150835/148‑1 (MQ=255)
                                                    tGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGaa                                                                                              >  1:3164295/1‑148 (MQ=255)
                                                        aaaaCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATc                                                                                          >  2:1013940/1‑148 (MQ=255)
                                                           aCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTCAGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAat                                                                                       >  1:1003463/1‑148 (MQ=255)
                                                            ccGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAata                                                                                      >  1:3079704/1‑148 (MQ=255)
                                                            ccGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAata                                                                                      >  2:3259633/1‑148 (MQ=255)
                                                            ccGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAata                                                                                      >  2:1622463/1‑148 (MQ=255)
                                                             cgcgCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAatat                                                                                     <  1:2699760/148‑1 (MQ=255)
                                                              gcgcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATAtt                                                                                    <  2:35915/148‑1 (MQ=255)
                                                               cgcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATtc                                                                                   <  2:2036315/148‑1 (MQ=255)
                                                                gcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATtct                                                                                  <  2:3023557/148‑1 (MQ=255)
                                                                 aaaaaTCACCATCTTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATtctc                                                                                 <  1:2779548/147‑1 (MQ=255)
                                                                  aaaaTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTctct                                                                                <  1:2948011/148‑1 (MQ=255)
                                                                    aaTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCttt                                                                              >  2:3126701/1‑148 (MQ=255)
                                                                    aaTCAACACCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCttt                                                                              >  1:607674/1‑148 (MQ=255)
                                                                     aTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTg                                                                             >  1:1604220/1‑148 (MQ=255)
                                                                      tCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGc                                                                            >  2:1139955/1‑148 (MQ=255)
                                                                          cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                        >  2:3280510/1‑148 (MQ=255)
                                                                          cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                        <  2:24057/148‑1 (MQ=255)
                                                                          cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                        >  2:2183230/1‑148 (MQ=255)
                                                                          cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                        <  2:1447613/148‑1 (MQ=255)
                                                                            tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAAt                                                                      >  2:188808/1‑148 (MQ=255)
                                                                            tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAAt                                                                      <  1:2657649/148‑1 (MQ=255)
                                                                            tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCGTTGCGGCAAt                                                                      >  2:1859075/1‑148 (MQ=255)
                                                                             cGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATg                                                                     <  2:712048/148‑1 (MQ=255)
                                                                             cGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATg                                                                     <  2:803310/148‑1 (MQ=255)
                                                                              gTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATgg                                                                    <  1:2450675/148‑1 (MQ=255)
                                                                               tCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGt                                                                   >  1:3264922/1‑148 (MQ=255)
                                                                                    atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCgaga                                                              <  1:63487/148‑1 (MQ=255)
                                                                                     ttCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGaa                                                             <  1:528212/148‑1 (MQ=255)
                                                                                         aTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaaa                                                         <  1:2231238/148‑1 (MQ=255)
                                                                                         aTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaaa                                                         >  1:2884652/1‑148 (MQ=255)
                                                                                          tttCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAt                                                        >  1:1591303/1‑148 (MQ=255)
                                                                                          tttCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAt                                                        <  1:1574920/148‑1 (MQ=255)
                                                                                            tCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTg                                                      <  2:2887491/148‑1 (MQ=255)
                                                                                             ccACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGc                                                     <  1:2147459/148‑1 (MQ=255)
                                                                                                 aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTg                                                 >  1:2594734/1‑148 (MQ=255)
                                                                                                    cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCgg                                              <  2:1213789/148‑1 (MQ=255)
                                                                                                        cATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATc                                          <  2:2707850/148‑1 (MQ=255)
                                                                                                         aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCt                                         <  1:2682230/148‑1 (MQ=255)
                                                                                                         aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCt                                         <  2:2448218/148‑1 (MQ=255)
                                                                                                          ttACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                        >  1:932403/1‑148 (MQ=255)
                                                                                                           tACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                       >  2:1117270/1‑148 (MQ=255)
                                                                                                               gTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCa                                   <  1:1884540/148‑1 (MQ=255)
                                                                                                                tttGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCaa                                  >  2:3298816/1‑148 (MQ=255)
                                                                                                                 ttGTGACAAACGTCCGCTCGCCATCCTCCGTCTCCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAAt                                 <  2:1075423/148‑1 (MQ=255)
                                                                                                                      aCAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGAc                            >  2:374696/1‑148 (MQ=255)
                                                                                                                       cAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGTGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGCCt                           <  2:3284888/148‑1 (MQ=255)
                                                                                                                         aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCa                         <  2:1432198/148‑1 (MQ=255)
                                                                                                                         aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCa                         >  2:3200487/1‑148 (MQ=255)
                                                                                                                            gTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTa                      >  2:2257664/1‑148 (MQ=255)
                                                                                                                             tCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTaa                     >  1:1118836/1‑148 (MQ=255)
                                                                                                                              ccGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                    >  1:1025943/1‑148 (MQ=255)
                                                                                                                              ccGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                    >  1:506109/1‑148 (MQ=255)
                                                                                                                                    gCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgtg              <  1:1620007/148‑1 (MQ=255)
                                                                                                                                    gCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgtg              <  2:2465592/148‑1 (MQ=255)
                                                                                                                                     ccATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGc             >  2:2994648/1‑148 (MQ=255)
                                                                                                                                     ccATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGc             >  2:250496/1‑148 (MQ=255)
                                                                                                                                      cATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCg            >  1:3241126/1‑148 (MQ=255)
                                                                                                                                        tcctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGAt          <  1:2714500/148‑1 (MQ=255)
                                                                                                                                        tcctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGAt          >  2:297357/1‑148 (MQ=255)
                                                                                                                                            ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGcc      <  1:2540838/148‑1 (MQ=255)
                                                                                                                                            ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGcc      <  1:820749/148‑1 (MQ=255)
                                                                                                                                            ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGcc      >  1:1815011/1‑148 (MQ=255)
                                                                                                                                             cGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGccc     <  1:1957325/148‑1 (MQ=255)
                                                                                                                                                cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGa  <  1:23772/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGA  >  CP009273/1850736‑1851027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑