Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 173,725 G→T 90.5% G129G (GGC→GGA)  yadS ← UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,7250GT90.5% 411.6 / 38.0 158G129G (GGC→GGA) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (7/8);  new base T (78/65);  total (85/73)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 160 total aligned reads displayed.

CATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGG  >  CP009273/173581‑173868
                                                                                                                                                |                                                                                                                                               
catACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCa                                                                                                                                              >  2:2138356/1‑148 (MQ=255)
 atACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCaa                                                                                                                                             <  2:131688/148‑1 (MQ=255)
 atACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCaa                                                                                                                                             <  2:131752/148‑1 (MQ=255)
  tACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCCGAACATCACGAATTATCCCGCCTCCAAc                                                                                                                                            >  1:501731/1‑148 (MQ=255)
  tACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAAc                                                                                                                                            >  1:822951/1‑148 (MQ=255)
   aCTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACg                                                                                                                                           <  1:16699/148‑1 (MQ=255)
   aCTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACg                                                                                                                                           <  2:1477615/148‑1 (MQ=255)
    cTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGc                                                                                                                                          >  2:595396/1‑148 (MQ=255)
        cTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGt                                                                                                                                      >  1:2789957/1‑148 (MQ=255)
        cTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGt                                                                                                                                      <  2:326371/148‑1 (MQ=255)
         tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                     >  2:2051628/1‑148 (MQ=255)
         tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCATCCAACGCCAGTa                                                                                                                                     <  1:237754/148‑1 (MQ=255)
          gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTaa                                                                                                                                    <  2:1254693/148‑1 (MQ=255)
           tttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAAt                                                                                                                                   >  1:1196967/1‑148 (MQ=255)
            ttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                  >  2:3255830/1‑148 (MQ=255)
            ttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                  <  2:1984395/148‑1 (MQ=255)
            ttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                  <  2:1985189/148‑1 (MQ=255)
            ttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                  <  1:1457437/148‑1 (MQ=255)
                     tACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATa                                                                                                                         <  1:2141519/148‑1 (MQ=255)
                     tACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATa                                                                                                                         >  2:53927/1‑148 (MQ=255)
                        ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaa                                                                                                                      <  1:342647/148‑1 (MQ=255)
                         gAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                     <  1:2269509/148‑1 (MQ=255)
                         gAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                     <  1:2786776/148‑1 (MQ=255)
                         gAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACaaa                                                                                                                     <  2:3118100/148‑1 (MQ=255)
                                  taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                            <  1:3214800/148‑1 (MQ=255)
                                  taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                            <  1:3200755/148‑1 (MQ=255)
                                  taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                            <  2:1199606/148‑1 (MQ=255)
                                  taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                            <  1:1615527/148‑1 (MQ=255)
                                   aataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTaa                                                                                                           <  2:328653/148‑1 (MQ=255)
                                    ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                          >  2:2585603/1‑148 (MQ=255)
                                    ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                          >  2:1244721/1‑148 (MQ=255)
                                    ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                          >  2:1244768/1‑148 (MQ=255)
                                    ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                          >  2:2202130/1‑148 (MQ=255)
                                    ataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAAc                                                                                                          >  2:2202070/1‑148 (MQ=255)
                                     taaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACg                                                                                                         >  2:3186376/1‑148 (MQ=255)
                                           gTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCgg                                                                                                   <  2:3017350/148‑1 (MQ=255)
                                           gTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCgg                                                                                                   <  2:3017264/148‑1 (MQ=255)
                                            tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                  <  1:2078596/148‑1 (MQ=255)
                                             aGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCt                                                                                                 >  1:1511449/1‑148 (MQ=255)
                                             aGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCt                                                                                                 >  1:1511457/1‑148 (MQ=255)
                                              gCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCtt                                                                                                <  2:1490390/148‑1 (MQ=255)
                                               cGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTc                                                                                               <  1:2689392/148‑1 (MQ=255)
                                                  ggACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGc                                                                                            >  1:898244/1‑148 (MQ=255)
                                                    aCAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAt                                                                                          >  1:3245948/1‑148 (MQ=255)
                                                       aTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCTAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaa                                                                                       >  1:1879012/1‑148 (MQ=255)
                                                       aTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaa                                                                                       >  1:1230419/1‑148 (MQ=255)
                                                        tACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaaa                                                                                      >  2:1923568/1‑148 (MQ=255)
                                                          ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                    <  1:2601546/148‑1 (MQ=255)
                                                          ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                    <  1:2601647/148‑1 (MQ=255)
                                                          ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCAAGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                    <  1:445062/148‑1 (MQ=255)
                                                           cgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGc                                                                                   >  2:2466156/1‑148 (MQ=255)
                                                             ccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCtt                                                                                 >  2:919992/1‑148 (MQ=255)
                                                                ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                              >  1:1444216/1‑148 (MQ=255)
                                                                ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                              >  1:2805800/1‑148 (MQ=255)
                                                                 taataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAtt                                                                             >  2:777190/1‑148 (MQ=255)
                                                                  aataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTc                                                                            >  2:1119603/1‑148 (MQ=255)
                                                                     aCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACg                                                                         <  2:1113366/148‑1 (MQ=255)
                                                                        ggCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCa                                                                      >  1:237588/1‑148 (MQ=255)
                                                                                  tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaa                                                            <  1:2764984/148‑1 (MQ=255)
                                                                                  tAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaa                                                            >  1:296694/1‑148 (MQ=255)
                                                                                    gATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                          >  2:2253722/1‑148 (MQ=255)
                                                                                     aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                         <  1:3132290/148‑1 (MQ=255)
                                                                                      tttCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                  >  1:146873/1‑106 (MQ=255)
                                                                                        tCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCg                                                      >  2:493010/1‑148 (MQ=255)
                                                                                        tCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCg                                                      >  2:2668445/1‑148 (MQ=255)
                                                                                         cTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGc                                                     <  1:3190913/148‑1 (MQ=255)
                                                                                           gTACGTAATATCATGGGGATTTCGCGGGCCAGAACATCACTAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCTATTAACGGACCGGCTTCCGCATTAAAGGATTTATTCACGCCAATGCCGACAAACACCGCCa                                                   <  1:3263755/148‑1 (MQ=255)
                                                                                           gTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCa                                                   >  2:2758953/1‑148 (MQ=255)
                                                                                                  aaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAc                                            >  1:2817746/1‑148 (MQ=255)
                                                                                                  aaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAc                                            >  1:2561941/1‑148 (MQ=255)
                                                                                                        tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTc                                      <  1:2288816/148‑1 (MQ=255)
                                                                                                        tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTc                                      <  2:3135761/148‑1 (MQ=255)
                                                                                                           ggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                   >  2:3099748/1‑148 (MQ=255)
                                                                                                                   cgGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCa                           <  2:1035746/148‑1 (MQ=255)
                                                                                                                     ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                         >  1:500853/1‑148 (MQ=255)
                                                                                                                     ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                         >  2:2465298/1‑148 (MQ=255)
                                                                                                                       ccAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACAt                       >  2:579231/1‑148 (MQ=255)
                                                                                                                       ccAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACAt                       >  2:2024952/1‑148 (MQ=255)
                                                                                                                        cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAACCCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                      <  2:512486/148‑1 (MQ=255)
                                                                                                                        cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                      <  1:929093/148‑1 (MQ=255)
                                                                                                                        cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                      <  1:658891/148‑1 (MQ=255)
                                                                                                                        cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                      <  2:2303351/148‑1 (MQ=255)
                                                                                                                        cAGAACAACACGACTGAACCCGCCTCCAACGCCAGTAATGACGCCCATCCAACCCGCGATTACCGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                      <  2:2409237/148‑1 (MQ=255)
                                                                                                                           aaCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAt                   >  2:1388602/1‑148 (MQ=255)
                                                                                                                           aaCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAt                   >  2:3020420/1‑148 (MQ=255)
                                                                                                                            aCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAtt                  >  1:1789094/1‑148 (MQ=255)
                                                                                                                            aCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAtt                  >  1:216735/1‑148 (MQ=255)
                                                                                                                             cATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAttt                 >  2:1912117/1‑148 (MQ=255)
                                                                                                                               tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTg               >  2:2866828/1‑148 (MQ=255)
                                                                                                                                cACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg              <  1:1923568/148‑1 (MQ=255)
                                                                                                                                cACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg              >  1:486818/1‑148 (MQ=255)
                                                                                                                                cACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg              <  2:1018699/148‑1 (MQ=255)
                                                                                                                                  cGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTa            <  1:3169276/148‑1 (MQ=255)
                                                                                                                                  cGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTa            <  1:2668445/148‑1 (MQ=255)
                                                                                                                                    aaTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAg          >  2:1891094/1‑148 (MQ=255)
                                                                                                                                      tGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCg        >  1:3007264/1‑148 (MQ=255)
                                                                                                                                      tGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCg        >  1:3007248/1‑148 (MQ=255)
                                                                                                                                        aTCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTc      <  2:1196967/148‑1 (MQ=255)
                                                                                                                                            cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACACACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTCAGCGTCTtgg  >  2:2833009/1‑148 (MQ=255)
                                                                                                                                            cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg  >  2:75386/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGG  >  CP009273/173581‑173868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑