Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,870,733 T→G 16.4% L268L (CTT→CTG)  yeaN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,870,7330TG16.4% 334.3 / 79.2 165L268L (CTT→CTG) yeaNputative transporter
Reads supporting (aligned to +/- strand):  ref base T (77/60);  new base G (14/13);  total (92/73)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 168 total aligned reads displayed.

TGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATA  >  CP009273/1870589‑1870880
                                                                                                                                                |                                                                                                                                                   
    aTCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAc                                                                                                                                              <  1:2518940/148‑1 (MQ=255)
      cAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcat                                                                                                                                            >  2:873051/1‑148 (MQ=255)
      cAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTAcat                                                                                                                                            >  2:1374605/1‑148 (MQ=255)
      cAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTAcat                                                                                                                                            >  2:1993248/1‑148 (MQ=255)
        aCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataa                                                                                                                                          >  1:1657290/1‑146 (MQ=255)
        aCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataa                                                                                                                                          >  1:1657272/1‑146 (MQ=255)
          tCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGTGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtg                                                                                                                                        <  2:2640781/148‑1 (MQ=255)
            aCTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtgtg                                                                                                                                      >  1:1641539/1‑148 (MQ=255)
               ggTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaaa                                                                                                                                   >  1:711213/1‑148 (MQ=255)
               ggTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaaa                                                                                                                                   <  2:3265499/148‑1 (MQ=255)
                gTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAg                                                                                                                                  <  1:1129297/148‑1 (MQ=255)
                 tCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGa                                                                                                                                 <  1:2647118/148‑1 (MQ=255)
                  cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAt                                                                                                                                >  1:208884/1‑148 (MQ=255)
                  cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAt                                                                                                                                >  1:2618704/1‑148 (MQ=255)
                      tACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGc                                                                                                                            >  2:142700/1‑148 (MQ=255)
                      aaCGTGATTATTGGCTGGCTACCTGCGATCCTCATCATTCACGGATATAGCGAAGAACAGGCGGGTTCACTGCATGGTTTGCTGCAACTACCCACAGCAGCACCCGGTTTGCTGATCCCACATTTCTTACATCATGTTAAAGATCAGc                                                                                                                            >  2:125852/2‑148 (MQ=255)
                       aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                           <  1:1961015/148‑1 (MQ=255)
                         gTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTg                                                                                                                         <  2:2816687/148‑1 (MQ=255)
                          tGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTgg                                                                                                                        >  2:1591777/1‑148 (MQ=255)
                             ttattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAt                                                                                                                     <  1:1638109/148‑1 (MQ=255)
                              tattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAtt                                                                                                                    >  2:839280/1‑148 (MQ=255)
                              tattGGCTGGCTTCCGGCGATCCTCATCAGTAACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGTTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAtt                                                                                                                    >  2:2991218/1‑148 (MQ=255)
                                ttGGCTGGCGTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGc                                                                                                                  <  2:2394354/148‑1 (MQ=255)
                                    ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                              >  1:2572756/1‑148 (MQ=255)
                                    ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                              >  1:1128170/1‑148 (MQ=255)
                                    ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                              >  1:2915388/1‑148 (MQ=255)
                                    ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                              >  1:2915201/1‑148 (MQ=255)
                                     tggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgt                                                                                                             <  1:2971609/148‑1 (MQ=255)
                                      ggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgtt                                                                                                            >  2:1433794/1‑148 (MQ=255)
                                      ggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgtt                                                                                                            <  1:1134475/148‑1 (MQ=255)
                                        ctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcg                                                                                                          >  1:1071950/1‑148 (MQ=255)
                                        ctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcg                                                                                                          <  2:2661380/148‑1 (MQ=255)
                                         ttCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTGTGCTGCAACTAGCCACAGCAGCGCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcgt                                                                                                         <  2:2226872/148‑1 (MQ=255)
                                             ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGcc                                                                                                     >  2:757986/1‑148 (MQ=255)
                                               cGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCtt                                                                                                   >  1:177186/1‑148 (MQ=255)
                                                gATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTg                                                                                                  <  2:277573/148‑1 (MQ=255)
                                                   ccTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtg                                                                                               <  2:3014213/148‑1 (MQ=255)
                                                     tcatcaGTCACGGCTAGAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgtg                                                                                             <  1:3186020/148‑1 (MQ=255)
                                                        tcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgcgc                                                                                          >  1:2470131/1‑148 (MQ=255)
                                                        tcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGACACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAACAGCGTGGTATTGCAGCGTTCGTTGCCTAGATGTgcgc                                                                                          >  1:811039/1‑148 (MQ=255)
                                                         caGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCa                                                                                         >  1:2767498/1‑148 (MQ=255)
                                                          aGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAg                                                                                        <  1:1552693/148‑1 (MQ=255)
                                                            tCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTg                                                                                      <  2:29505/148‑1 (MQ=255)
                                                               cGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgc                                                                                   >  2:195151/1‑148 (MQ=255)
                                                                   tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCgg                                                                               <  1:805740/148‑1 (MQ=255)
                                                                    ataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGt                                                                              <  1:646068/148‑1 (MQ=255)
                                                                      aGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTg                                                                            >  2:3091556/1‑148 (MQ=255)
                                                                       gCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                           <  1:977499/148‑1 (MQ=255)
                                                                       gCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                           >  1:673287/1‑148 (MQ=255)
                                                                          aaGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGct                                                                        <  2:251158/148‑1 (MQ=255)
                                                                             cacaGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTg                                                                     <  1:142700/148‑1 (MQ=255)
                                                                                 ggCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCttt                                                                 >  2:1089982/1‑148 (MQ=255)
                                                                                  gCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTa                                                                <  2:1657290/148‑1 (MQ=255)
                                                                                   cGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTAt                                                               <  1:36454/148‑1 (MQ=255)
                                                                                    gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATg                                                              <  2:2671035/148‑1 (MQ=255)
                                                                                    gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATg                                                              <  2:3145791/148‑1 (MQ=255)
                                                                                    gggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCGGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGGTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATg                                                              <  2:2540066/148‑1 (MQ=255)
                                                                                     ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                             >  2:2241003/1‑148 (MQ=255)
                                                                                     ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                             <  1:2141686/148‑1 (MQ=255)
                                                                                          aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcg                                                        >  1:2093765/1‑148 (MQ=255)
                                                                                              cATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcg                                                    >  1:3163561/1‑148 (MQ=255)
                                                                                              cATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGATTGCTGATCCCACAATTCTTACATAATGTGAAAGATCAGCGAGGTATTGCAGCTTTCGTTGCCTTGATGTGCGCAGTTGTCGCGGTTGGGCTCTGCTGTATGCCAGCGCAcg                                                    >  2:2484082/1‑148 (MQ=255)
                                                                                                tGGTTTGCTGCAACTAGCCACAGCAGCACCCGGATTGCTGATCCCACTTTACTTACATAATGTGAAAGATCAGCGTGGTATAGCAGCGTTCGTTGCATTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcgcg                                                  >  1:1256796/1‑148 (MQ=255)
                                                                                                         gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                         >  2:3268092/1‑148 (MQ=255)
                                                                                                          cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                        >  2:3098776/1‑148 (MQ=255)
                                                                                                            aCTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAct                                      <  1:1591777/148‑1 (MQ=255)
                                                                                                            aCTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAct                                      <  1:1190070/148‑1 (MQ=255)
                                                                                                             cTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGActc                                     >  2:1483440/1‑148 (MQ=255)
                                                                                                             cTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGActc                                     <  1:1374605/148‑1 (MQ=255)
                                                                                                             cTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGActc                                     <  1:1993248/148‑1 (MQ=255)
                                                                                                                gCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGc                                  >  2:1352337/1‑148 (MQ=255)
                                                                                                                 ccACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                 >  2:111401/1‑148 (MQ=255)
                                                                                                                 ccACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                 >  1:2038429/1‑148 (MQ=255)
                                                                                                                  cacaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtt                                <  2:1482891/148‑1 (MQ=255)
                                                                                                                   acaGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCttt                               >  2:474806/1‑148 (MQ=255)
                                                                                                                    cagcagCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtttt                              >  1:1745057/1‑148 (MQ=255)
                                                                                                                        agcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTTCTGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGt                          <  2:208884/148‑1 (MQ=255)
                                                                                                                          caCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGttt                        >  1:499014/1‑148 (MQ=255)
                                                                                                                            cccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                      >  2:2748017/1‑148 (MQ=255)
                                                                                                                            cccGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                      >  2:2991574/1‑148 (MQ=255)
                                                                                                                            cccGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                      >  2:2991622/1‑148 (MQ=255)
                                                                                                                             ccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTgg                     <  1:1075382/148‑1 (MQ=255)
                                                                                                                              cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                    >  2:966745/1‑148 (MQ=255)
                                                                                                                              cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                    >  2:739650/1‑148 (MQ=255)
                                                                                                                               ggTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGtt                   <  1:121268/148‑1 (MQ=255)
                                                                                                                                       gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca           >  1:1762572/1‑148 (MQ=255)
                                                                                                                                       gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca           >  1:1014838/1‑148 (MQ=255)
                                                                                                                                       gATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca           >  1:1886514/1‑148 (MQ=255)
                                                                                                                                        aTCCCACTTTTCTTACATAATGTAAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa          >  2:2111737/1‑148 (MQ=255)
                                                                                                                                        aTCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa          <  2:2915388/148‑1 (MQ=255)
                                                                                                                                        aTCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa          <  2:2915201/148‑1 (MQ=255)
                                                                                                                                         tCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac         >  1:3048500/1‑148 (MQ=255)
                                                                                                                                         tCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac         >  2:818552/1‑148 (MQ=255)
                                                                                                                                          cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca        >  2:189044/1‑148 (MQ=255)
                                                                                                                                          cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca        <  1:431711/148‑1 (MQ=255)
                                                                                                                                          cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca        >  2:2236607/1‑148 (MQ=255)
                                                                                                                                          cccACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca        >  1:2494432/1‑148 (MQ=255)
                                                                                                                                          cccACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca        >  1:2494521/1‑148 (MQ=255)
                                                                                                                                           ccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaacaa       <  2:837283/148‑1 (MQ=255)
                                                                                                                                                tttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa  >  1:2980715/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATA  >  CP009273/1870589‑1870880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑