BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,872,054	A→T	85.5%	E28V (GAG→GTG)	yeaP →	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,872,054	0	A	T	85.5%	464.8 / 61.6	165	E28V (GAG→GTG)	yeaP	diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base A (15/9); new base T (64/77); total (79/86)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 167 total aligned reads displayed.

GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTT  >  CP009273/1871909‑1872198

gTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGgtga                                                                                                                                                >  1:684201/1‑148 (MQ=255)

       cATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGa                                                                                                                                         <  1:654395/148‑1 (MQ=255)

           ccTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATg                                                                                                                                     <  1:1859097/148‑1 (MQ=255)

                 aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACt                                                                                                                               >  2:656818/1‑148 (MQ=255)

                    aTTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                            <  1:2266825/148‑1 (MQ=255)

                    aTTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                            <  1:2762387/148‑1 (MQ=255)

                     ttAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGata                                                                                                                           >  1:1836336/1‑148 (MQ=255)

                      tAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGatat                                                                                                                          >  1:540030/1‑148 (MQ=255)

                         ataatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGa                                                                                                                       >  2:1276682/1‑148 (MQ=255)

                           aatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAt                                                                                                                     <  2:8226/148‑1 (MQ=255)

                           aatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAt                                                                                                                     <  1:2816636/148‑1 (MQ=255)

                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                  >  2:3074655/1‑148 (MQ=255)

                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                  >  2:2529196/1‑148 (MQ=255)

                                 cATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                               >  2:3228768/1‑148 (MQ=255)

                                    ttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                            >  1:664131/1‑148 (MQ=255)

                                      aCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTg                                                                                                          <  1:153045/148‑1 (MQ=255)

                                       cccGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGa                                                                                                         <  2:3227796/148‑1 (MQ=255)

                                           ccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGAGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaa                                                                                                     <  1:1569462/148‑1 (MQ=255)

                                            cgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaaa                                                                                                    >  2:1040064/1‑148 (MQ=255)

                                              tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt                                                                                                  <  1:724539/148‑1 (MQ=255)

                                                tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                <  1:931242/148‑1 (MQ=255)

                                                tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                <  1:2320781/148‑1 (MQ=255)

                                                tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                <  1:2256609/148‑1 (MQ=255)

                                                   tGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATg                                                                                             >  1:1832553/1‑148 (MQ=255)

                                                   tGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATg                                                                                             <  2:1027024/148‑1 (MQ=255)

                                                     gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                           >  1:2011413/1‑148 (MQ=255)

                                                     gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                           >  2:1184508/1‑148 (MQ=255)

                                                     gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                           >  2:1184592/1‑148 (MQ=255)

                                                       gttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGa                                                                                         <  2:1312103/148‑1 (MQ=255)

                                                           ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcg                                                                                     >  1:2528733/1‑148 (MQ=255)

                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                  >  2:1082563/1‑148 (MQ=255)

                                                                  tcagatcagaTTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgc                                                                              >  2:3124583/1‑148 (MQ=255)

                                                                   cagatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgca                                                                             >  2:3145330/1‑148 (MQ=255)

                                                                    agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcag                                                                            <  2:1600134/148‑1 (MQ=255)

                                                                     gatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAGATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagc                                                                           >  1:1919381/1‑148 (MQ=255)

                                                                       tcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCat                                                                         >  2:2711820/1‑148 (MQ=255)

                                                                       tcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCat                                                                         >  2:2711804/1‑148 (MQ=255)

                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     >  2:2151663/1‑148 (MQ=255)

                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     >  2:488722/1‑148 (MQ=255)

                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     <  1:1382936/148‑1 (MQ=255)

                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     >  1:1495243/1‑148 (MQ=255)

                                                                             tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATg                                                                   >  2:671391/1‑148 (MQ=255)

                                                                              aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGt                                                                  <  2:192633/148‑1 (MQ=255)

                                                                              aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGt                                                                  <  1:156392/148‑1 (MQ=255)

                                                                              aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGt                                                                  <  1:3083716/148‑1 (MQ=255)

                                                                                cGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGttt                                                                <  2:2486314/148‑1 (MQ=255)

                                                                                 gCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTg                                                               <  2:1225825/148‑1 (MQ=255)

                                                                                 gCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTg                                                               <  1:2414783/148‑1 (MQ=255)

                                                                                   ccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGcc                                                             <  1:1028738/148‑1 (MQ=255)

                                                                                       gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCTCTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTa                                                         <  1:2661279/148‑1 (MQ=255)

                                                                                        tctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                        >  1:2267601/1‑148 (MQ=255)

                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                       <  1:2532060/148‑1 (MQ=255)

                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGACACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                       >  1:393674/1‑148 (MQ=255)

                                                                                          tcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACa                                                      <  2:1651593/148‑1 (MQ=255)

                                                                                          tcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACa                                                      >  1:1959664/1‑148 (MQ=255)

                                                                                           cGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAg                                                     >  2:1277350/1‑148 (MQ=255)

                                                                                             cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGcc                                                   <  2:2765667/148‑1 (MQ=255)

                                                                                               aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                                 >  2:2908217/1‑148 (MQ=255)

                                                                                               aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                                 >  2:890859/1‑148 (MQ=255)

                                                                                                tCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGa                                                >  2:199106/1‑148 (MQ=255)

                                                                                                   cTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                             >  2:1473398/1‑148 (MQ=255)

                                                                                                     tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATg                                           >  2:40299/1‑148 (MQ=255)

                                                                                                     tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATg                                           >  1:2757508/1‑148 (MQ=255)

                                                                                                        cAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                        >  1:2626994/1‑148 (MQ=255)

                                                                                                        cAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                        >  1:2626739/1‑148 (MQ=255)

                                                                                                        cAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                        <  2:619795/148‑1 (MQ=255)

                                                                                                        cAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                        <  2:815009/148‑1 (MQ=255)

                                                                                                         aaaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACa                                       <  2:921425/148‑1 (MQ=255)

                                                                                                         aaaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACa                                       <  2:2256873/148‑1 (MQ=255)

                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      <  2:85445/148‑1 (MQ=255)

                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      <  2:452877/148‑1 (MQ=255)

                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                     <  1:65056/148‑1 (MQ=255)

                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                     <  2:3143320/148‑1 (MQ=255)

                                                                                                             aaCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                   >  1:1562400/1‑148 (MQ=255)

                                                                                                               cAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCgg                                 >  1:2556368/1‑148 (MQ=255)

                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              <  2:1545570/148‑1 (MQ=255)

                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              >  2:265930/1‑148 (MQ=255)

                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              <  2:2891448/148‑1 (MQ=255)

                                                                                                                   cACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGaa                             >  1:1672671/1‑148 (MQ=255)

                                                                                                                     cTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtt                           <  1:1164437/148‑1 (MQ=255)

                                                                                                                     cTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtt                           <  1:1189781/148‑1 (MQ=255)

                                                                                                                     cTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtt                           <  1:3102222/148‑1 (MQ=255)

                                                                                                                      tGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAttt                          <  1:1719531/148‑1 (MQ=255)

                                                                                                                      tGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAttt                          <  2:2285921/148‑1 (MQ=255)

                                                                                                                      tGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAttt                          <  2:622959/148‑1 (MQ=255)

                                                                                                                       ggAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtttt                         <  2:1828663/148‑1 (MQ=255)

                                                                                                                         aaaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAc                       >  2:3023896/1‑148 (MQ=255)

                                                                                                                          aaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                      >  1:2428044/1‑148 (MQ=255)

                                                                                                                           aGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCg                     >  2:1660344/1‑148 (MQ=255)

                                                                                                                                    cGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTggg            <  2:3217795/148‑1 (MQ=255)

                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt         >  1:1521522/1‑148 (MQ=255)

                                                                                                                                        aGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTa        <  2:863912/148‑1 (MQ=255)

                                                                                                                                          cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt      >  1:2223369/1‑148 (MQ=255)

                                                                                                                                          cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt      >  1:1689753/1‑148 (MQ=255)

                                                                                                                                          cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt      <  1:3218407/148‑1 (MQ=255)

                                                                                                                                          cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt      >  1:560359/1‑148 (MQ=255)

                                                                                                                                           ttCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTc     >  2:522411/1‑148 (MQ=255)

                                                                                                                                            tCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCACAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCg    <  1:1019938/148‑1 (MQ=255)

                                                                                                                                            tCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCg    <  2:3065557/148‑1 (MQ=255)

                                                                                                                                              tGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGtt  <  2:2361696/148‑1 (MQ=255)

GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTT  >  CP009273/1871909‑1872198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`