BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,874,924	T→A	92.5%	V31V (GTA→GTT)	leuE ←	leucine efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,874,924	0	T	A	92.5%	611.8 / 30.8	198	V31V (GTA→GTT)	leuE	leucine efflux protein
Reads supporting (aligned to +/- strand): ref base T (10/5); new base A (83/100); total (93/105)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

GTACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATG  >  CP009273/1874779‑1875066

gTACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAAc                                                                                                                                              <  1:253951/148‑1 (MQ=255)

  aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaa                                                                                                                                            >  1:919421/1‑148 (MQ=255)

  aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaa                                                                                                                                            >  1:3253432/1‑148 (MQ=255)

   cAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaaa                                                                                                                                           >  1:2270413/1‑148 (MQ=255)

         tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGt                                                                                                                                     >  1:636051/1‑148 (MQ=255)

          gAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTa                                                                                                                                    >  1:2449346/1‑148 (MQ=255)

             taataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTAttt                                                                                                                                 <  1:360950/148‑1 (MQ=255)

              aataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTg                                                                                                                                >  2:2944878/1‑148 (MQ=255)

               atatCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTgg                                                                                                                               >  1:2579425/1‑148 (MQ=255)

                 atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGcc                                                                                                                             <  2:1224607/148‑1 (MQ=255)

                 atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGcc                                                                                                                             <  2:374148/148‑1 (MQ=255)

                     gggTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTgg                                                                                                                         >  2:2924220/1‑148 (MQ=255)

                        tggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCAc                                                                                                                      <  2:1732266/148‑1 (MQ=255)

                           tCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCaa                                                                                                                   <  1:755650/148‑1 (MQ=255)

                           tCTAAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCaa                                                                                                                   >  1:1363679/1‑148 (MQ=255)

                               aattaatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAAcaca                                                                                                               >  1:1639151/1‑148 (MQ=255)

                                attaatGTCGCCACTCCAGCCCATGCCAGAAACATCAAAACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCAAACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACaa                                                                                                              >  1:2531964/1‑148 (MQ=255)

                                  taatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATa                                                                                                            >  2:792373/1‑148 (MQ=255)

                                   aatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaa                                                                                                           <  2:1318035/148‑1 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAANCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         >  1:1681833/1‑148 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         >  1:1792583/1‑148 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         >  1:3272499/1‑148 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         <  1:1454809/148‑1 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         <  1:2351871/148‑1 (MQ=255)

                                     tGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                         <  1:2352024/148‑1 (MQ=255)

                                        cGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATg                                                                                                      <  2:2084030/148‑1 (MQ=255)

                                        cGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATg                                                                                                      <  2:2738127/148‑1 (MQ=255)

                                        cGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATg                                                                                                      >  2:2538448/1‑148 (MQ=255)

                                           cACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGcc                                                                                                   <  2:2444611/148‑1 (MQ=255)

                                                  gCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCa                                                                                            >  2:1829701/1‑148 (MQ=255)

                                                   cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                           >  1:2178169/1‑148 (MQ=255)

                                                   cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                           >  1:1149214/1‑148 (MQ=255)

                                                   cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                           <  2:889927/148‑1 (MQ=255)

                                                   cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                           >  1:1301243/1‑148 (MQ=255)

                                                      aTGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATa                                                                                        <  1:1527387/148‑1 (MQ=255)

                                                      aTGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATa                                                                                        >  1:1455818/1‑148 (MQ=255)

                                                      aTGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATa                                                                                        <  1:1850851/148‑1 (MQ=255)

                                                       tGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAg                                                                                       >  2:3090389/1‑148 (MQ=255)

                                                           aGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTcc                                                                                   >  2:698834/1‑148 (MQ=255)

                                                            gAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCa                                                                                  >  2:1669376/1‑148 (MQ=255)

                                                               aCATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGtc                                                                               >  2:3124533/1‑147 (MQ=255)

                                                                cATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                              <  1:414367/148‑1 (MQ=255)

                                                                cATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                              <  1:414558/148‑1 (MQ=255)

                                                                cATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                              <  1:270416/148‑1 (MQ=255)

                                                                cATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                              >  1:1046345/1‑148 (MQ=255)

                                                                   cAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTACAGTAATTc                                                                           <  1:713020/148‑1 (MQ=255)

                                                                       aCCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                       >  1:1213823/1‑148 (MQ=255)

                                                                        ccGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAc                                                                      >  1:1362364/1‑148 (MQ=255)

                                                                             tCGCCAATAAATACACCGCAGGCCGCAAGATAGCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGt                                                                 <  1:520005/148‑1 (MQ=255)

                                                                              cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTa                                                                >  1:1346211/1‑148 (MQ=255)

                                                                              cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                       <  1:192275/141‑1 (MQ=255)

                                                                              cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                       >  2:192275/1‑141 (MQ=255)

                                                                                ccAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGAACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAtt                                                              >  2:1197440/1‑148 (MQ=255)

                                                                                  aataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCa                                                            >  2:481732/1‑148 (MQ=255)

                                                                                  aataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCa                                                            >  2:1949257/1‑148 (MQ=255)

                                                                                   ataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                           <  1:538462/148‑1 (MQ=255)

                                                                                   ataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                           >  1:367850/1‑148 (MQ=255)

                                                                                     aaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCg                                                         >  1:3056334/1‑148 (MQ=255)

                                                                                     aaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCg                                                         >  2:728979/1‑148 (MQ=255)

                                                                                       ataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                       <  2:919421/148‑1 (MQ=255)

                                                                                       ataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                       <  2:12804/148‑1 (MQ=255)

                                                                                          cacCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAacac                                                    <  1:2934996/148‑1 (MQ=255)

                                                                                            ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                  >  2:1510916/1‑148 (MQ=255)

                                                                                            ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                  <  2:1607840/148‑1 (MQ=255)

                                                                                            ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                  >  1:2815076/1‑148 (MQ=255)

                                                                                               cAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCACCACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                               <  1:2634280/148‑1 (MQ=255)

                                                                                               cAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                               <  2:1301243/148‑1 (MQ=255)

                                                                                               cAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                               >  2:2196371/1‑148 (MQ=255)

                                                                                                 ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAAAATGGTATTTGGCCCTGGCACCAACACAATAAAAATGGACCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTAATCGAACACATTAAcc                                             >  1:1439686/1‑148 (MQ=255)

                                                                                                  gCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                            >  2:1579125/1‑148 (MQ=255)

                                                                                                    cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctct                                          <  2:3253432/148‑1 (MQ=255)

                                                                                                    cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctct                                          <  2:1597943/148‑1 (MQ=255)

                                                                                                    cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctct                                          <  1:3087238/148‑1 (MQ=255)

                                                                                                       aaGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTa                                       <  1:2638165/148‑1 (MQ=255)

                                                                                                       aaGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTa                                       >  2:2393604/1‑148 (MQ=255)

                                                                                                         gATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                     <  1:1296695/148‑1 (MQ=255)

                                                                                                          aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                    >  1:687945/1‑148 (MQ=255)

                                                                                                          aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACCGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                    <  1:1089532/148‑1 (MQ=255)

                                                                                                          aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                    <  2:2634666/148‑1 (MQ=255)

                                                                                                          aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                    >  2:382513/1‑148 (MQ=255)

                                                                                                            aaCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTAt                                  <  2:302994/148‑1 (MQ=255)

                                                                                                              ccGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                                <  1:872303/148‑1 (MQ=255)

                                                                                                              ccGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                                >  2:557316/1‑148 (MQ=255)

                                                                                                              ccGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                                <  2:636710/148‑1 (MQ=255)

                                                                                                               cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                               <  2:2011777/148‑1 (MQ=255)

                                                                                                               cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                               <  1:855292/148‑1 (MQ=255)

                                                                                                                 ccTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                             <  1:2962246/148‑1 (MQ=255)

                                                                                                                   tttCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGa                           <  2:1781281/148‑1 (MQ=255)

                                                                                                                    ttCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGAt                          >  1:456963/1‑148 (MQ=255)

                                                                                                                      cATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCa                        <  1:1374820/148‑1 (MQ=255)

                                                                                                                           cGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgcgc                   >  2:888647/1‑148 (MQ=255)

                                                                                                                            gCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                  <  1:2619356/148‑1 (MQ=255)

                                                                                                                             cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa                 >  2:1879350/1‑148 (MQ=255)

                                                                                                                              tACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGAt                <  2:1455790/148‑1 (MQ=255)

                                                                                                                              tACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGAt                <  2:1455941/148‑1 (MQ=255)

                                                                                                                               aCTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTAGATCATGCGCGAtt               >  1:63676/1‑148 (MQ=255)

                                                                                                                                    cGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAgg          <  2:558803/148‑1 (MQ=255)

                                                                                                                                        aTTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGaa      >  1:1906969/1‑148 (MQ=255)

                                                                                                                                         tttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat     <  1:3228276/148‑1 (MQ=255)

                                                                                                                                            ttGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATg  <  1:3302806/148‑1 (MQ=255)

GTACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATG  >  CP009273/1874779‑1875066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`