Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,901,236 C→A 14.1% D28Y (GAT→TAT)  rlmA ← 23S rRNA m(1)G745 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,901,2360CA14.1% 601.2 / 53.8 191D28Y (GAT→TAT) rlmA23S rRNA m(1)G745 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (88/76);  new base A (16/11);  total (104/87)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 193 total aligned reads displayed.

CAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGA  >  CP009273/1901095‑1901383
                                                                                                                                             |                                                                                                                                                   
cAATTGCATCACGCAGCGGCTGATAATGTCTGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTg                                                                                                                                               <  1:2894281/148‑1 (MQ=255)
cAATTGCATCACGCAGCGGCTGATAATGTCTGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTg                                                                                                                                               <  1:2510204/148‑1 (MQ=255)
  aTTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGAt                                                                                                                                             >  1:2615762/1‑148 (MQ=255)
   ttGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATg                                                                                                                                            <  2:504078/148‑1 (MQ=255)
      cATCACGCAGCGGCTGATAATGTCCGGCATCTAAGACTGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCg                                                                                                                                         <  1:1969301/148‑1 (MQ=255)
      cATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCg                                                                                                                                         <  2:2287615/148‑1 (MQ=255)
         cACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTg                                                                                                                                      >  2:1189591/1‑148 (MQ=255)
           cGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTggg                                                                                                                                    <  1:3038562/148‑1 (MQ=255)
             cAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGa                                                                                                                                  <  1:2911528/148‑1 (MQ=255)
               gCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACa                                                                                                                                >  1:3018032/1‑148 (MQ=255)
                cGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAg                                                                                                                               <  2:262476/148‑1 (MQ=255)
                cGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAg                                                                                                                               >  1:1043528/1‑148 (MQ=255)
                cGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAg                                                                                                                               >  1:1043203/1‑148 (MQ=255)
                   cTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGata                                                                                                                            >  1:1989437/1‑148 (MQ=255)
                          tGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGt                                                                                                                     >  2:683944/1‑148 (MQ=255)
                          tGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGt                                                                                                                     >  2:438473/1‑148 (MQ=255)
                          tGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGt                                                                                                                     <  1:1173187/148‑1 (MQ=255)
                             ccGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGtttt                                                                                                                  >  1:1994152/1‑148 (MQ=255)
                               ggCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGtttttt                                                                                                                >  1:162024/1‑148 (MQ=255)
                                  aTCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCa                                                                                                             <  1:3168082/148‑1 (MQ=255)
                                   tCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCAc                                                                                                            >  2:1395782/1‑148 (MQ=255)
                                     tAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCAcgc                                                                                                          >  1:445704/1‑148 (MQ=255)
                                        gAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGaa                                                                                                       >  2:1012501/1‑148 (MQ=255)
                                         aaTGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGaaa                                                                                                      >  2:350105/1‑148 (MQ=255)
                                          aTGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAag                                                                                                     <  1:1905157/148‑1 (MQ=255)
                                          aTGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAag                                                                                                     <  1:339281/148‑1 (MQ=255)
                                             cgcgGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAgg                                                                                                  <  2:3107409/148‑1 (MQ=255)
                                                ggcgTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTg                                                                                               <  1:805126/148‑1 (MQ=255)
                                                  cgTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGAt                                                                                             >  2:888402/1‑148 (MQ=255)
                                                   gTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATg                                                                                            <  1:3001776/148‑1 (MQ=255)
                                                    tGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATgg                                                                                           >  2:2942590/1‑148 (MQ=255)
                                                    tGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATgg                                                                                           <  2:232961/148‑1 (MQ=255)
                                                    tGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATgg                                                                                           >  2:2747592/1‑148 (MQ=255)
                                                    tGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATgg                                                                                           >  2:1040417/1‑148 (MQ=255)
                                                       ttGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCaa                                                                                        >  2:2070783/1‑148 (MQ=255)
                                                       ttGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGATGTTTTTTTCACGCGAAAGAGGCTGATGGCaa                                                                                        >  1:1800289/1‑148 (MQ=255)
                                                        tGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCaaa                                                                                       >  2:2202239/1‑148 (MQ=255)
                                                        tGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCaaa                                                                                       >  2:3089524/1‑148 (MQ=255)
                                                        tGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCaaa                                                                                       >  2:1477960/1‑148 (MQ=255)
                                                        tGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCaaa                                                                                       >  2:1136751/1‑148 (MQ=255)
                                                          catcatTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCCAAGt                                                                                     >  2:1895229/1‑148 (MQ=255)
                                                           atcatTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTg                                                                                    >  1:1849476/1‑148 (MQ=255)
                                                           atcatTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTg                                                                                    >  1:682453/1‑148 (MQ=255)
                                                             catTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGa                                                                                  >  1:406476/1‑148 (MQ=255)
                                                              atTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGAc                                                                                 <  1:1101373/148‑1 (MQ=255)
                                                                ttCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAg                                                                               >  1:1146641/1‑148 (MQ=255)
                                                                   cgcgCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGaa                                                                            >  2:34884/1‑148 (MQ=255)
                                                                       cTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAg                                                                        <  1:1550922/148‑1 (MQ=255)
                                                                        tGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGa                                                                       <  1:232233/148‑1 (MQ=255)
                                                                          tCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACa                                                                     <  1:702529/148‑1 (MQ=255)
                                                                            gCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATg                                                                   <  2:2480890/148‑1 (MQ=255)
                                                                             cccGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGa                                                                  <  2:1996867/148‑1 (MQ=255)
                                                                                ggATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACaa                                                               <  2:2212511/148‑1 (MQ=255)
                                                                                   tCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTc                                                            >  2:1773059/1‑148 (MQ=255)
                                                                                    cACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTcc                                                           <  2:2948416/148‑1 (MQ=255)
                                                                                    cACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTcc                                                           <  1:2297297/148‑1 (MQ=255)
                                                                                    cACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTcc                                                           <  1:2296536/148‑1 (MQ=255)
                                                                                     aCGAGACCGTTTATGCTGAACGGGCAGCAGATCGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCg                                                          >  2:321604/1‑148 (MQ=255)
                                                                                      cGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTAGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCgg                                                         <  1:2721659/148‑1 (MQ=255)
                                                                                        agaCCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCa                                                       >  1:459614/1‑148 (MQ=255)
                                                                                           ccGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGa                                                    >  1:2424281/1‑148 (MQ=255)
                                                                                            cGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGAt                                                   <  2:1549284/148‑1 (MQ=255)
                                                                                            cGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGAt                                                   <  2:165074/148‑1 (MQ=255)
                                                                                            cGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGAt                                                   <  2:1095353/148‑1 (MQ=255)
                                                                                             gTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATa                                                  <  2:3150252/148‑1 (MQ=255)
                                                                                              tttAAGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATAc                                                 >  2:790240/1‑148 (MQ=255)
                                                                                                tATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTa                                               <  1:440114/148‑1 (MQ=255)
                                                                                                   gCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCGGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAg                                            >  2:3026435/1‑148 (MQ=255)
                                                                                                         cGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCa                                      >  1:2800721/1‑148 (MQ=255)
                                                                                                           ggCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAg                                    >  2:2406611/1‑148 (MQ=255)
                                                                                                              agcagATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAgtgt                                 <  1:3143833/148‑1 (MQ=255)
                                                                                                                   aTTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAAcgc                            >  2:3051792/1‑148 (MQ=255)
                                                                                                                   aTTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAAcgc                            >  2:2589833/1‑148 (MQ=255)
                                                                                                                    ttGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAAcgcg                           >  1:3206648/1‑148 (MQ=255)
                                                                                                                     tGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGa                          <  1:1316424/148‑1 (MQ=255)
                                                                                                                      gACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGaa                         <  1:509068/148‑1 (MQ=255)
                                                                                                                       aCATACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACCACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAAt                        >  2:2698537/1‑148 (MQ=255)
                                                                                                                        cATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATc                       <  1:1618123/148‑1 (MQ=255)
                                                                                                                        cATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATc                       <  1:2741257/148‑1 (MQ=255)
                                                                                                                         aTACCCTTCTTTCGCCATATAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCa                      <  1:1189591/148‑1 (MQ=255)
                                                                                                                             ccTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACCGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCAcgcg                  >  2:41469/1‑148 (MQ=255)
                                                                                                                             ccTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCAcgcg                  <  1:2159799/148‑1 (MQ=255)
                                                                                                                              cttcttTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACgcgg                 <  1:760702/148‑1 (MQ=255)
                                                                                                                               ttcttTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACgcggc                >  1:2093148/1‑148 (MQ=255)
                                                                                                                               ttcttTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACgcggc                >  2:2576178/1‑148 (MQ=255)
                                                                                                                                tcttTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACgcggcg               <  1:635887/148‑1 (MQ=255)
                                                                                                                                 cttTCGCCATATCAAACTGATGTCGCTGGGGACAGAAATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCAGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGcggcgg              >  2:260901/1‑148 (MQ=255)
                                                                                                                                     cGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCaa          <  2:1792332/148‑1 (MQ=255)
                                                                                                                                       ccATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAat        >  2:3211341/1‑148 (MQ=255)
                                                                                                                                       ccATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAat        >  1:1256658/1‑148 (MQ=255)
                                                                                                                                         atatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAatat      >  2:2688462/1‑148 (MQ=255)
                                                                                                                                         atatAAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAatat      <  2:2858999/148‑1 (MQ=255)
                                                                                                                                          tatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATc     >  1:559675/1‑148 (MQ=255)
                                                                                                                                          tatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATc     <  2:1989437/148‑1 (MQ=255)
                                                                                                                                          tatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATc     >  1:2234609/1‑148 (MQ=255)
                                                                                                                                          tatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATc     <  2:321753/148‑1 (MQ=255)
                                                                                                                                          tatCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATc     >  1:2234904/1‑148 (MQ=255)
                                                                                                                                            tCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTCACGCGAATCACGCGGCGGGCAAATATCCg   >  1:405419/1‑148 (MQ=255)
                                                                                                                                            tCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCg   >  1:2071964/1‑148 (MQ=255)
                                                                                                                                             cAAACTGATGTCGCTGGGGACAGATATATCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGa  <  2:2404416/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCACGCGAAAGAGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGA  >  CP009273/1901095‑1901383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑