Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,049,111 C→A 89.6% T190T (ACC→ACA)  amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,049,1110CA89.6% 521.5 / 54.7 210T190T (ACC→ACA) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (7/15);  new base A (74/114);  total (81/129)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255
                                                                                                                                                 |                                                                                                                                                
tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAgg                                                                                                                                                >  2:2666263/1‑148 (MQ=255)
 aaCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGt                                                                                                                                               <  1:401016/148‑1 (MQ=255)
  aCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              >  2:1320039/1‑148 (MQ=255)
    tCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACg                                                                                                                                            >  1:2238698/1‑148 (MQ=255)
     cGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGc                                                                                                                                           >  1:60098/1‑148 (MQ=255)
       cTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCa                                                                                                                                         <  1:1252293/148‑1 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       >  2:895441/1‑148 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       <  1:476168/148‑1 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCGCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       <  2:1958626/148‑1 (MQ=255)
          cTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTCCGCCAGtt                                                                                                                                      >  2:2382314/1‑148 (MQ=255)
             cccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGaa                                                                                                                                   <  2:2259091/148‑1 (MQ=255)
             cccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGaa                                                                                                                                   <  2:1192595/148‑1 (MQ=255)
             cccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGaa                                                                                                                                   <  1:2735225/148‑1 (MQ=255)
               cGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACa                                                                                                                                 >  1:1879978/1‑148 (MQ=255)
               cGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACa                                                                                                                                 <  1:2581513/148‑1 (MQ=255)
                gACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                >  1:2024492/1‑148 (MQ=255)
                gACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                <  2:2510246/148‑1 (MQ=255)
                gACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                <  2:5918/148‑1 (MQ=255)
                 acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               <  2:3139080/148‑1 (MQ=255)
                 acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               <  2:3139226/148‑1 (MQ=255)
                  caacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACAttt                                                                                                                              <  2:572199/148‑1 (MQ=255)
                     caGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCa                                                                                                                           >  2:1983160/1‑148 (MQ=255)
                      aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAg                                                                                                                          <  2:2894530/148‑1 (MQ=255)
                         aCTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCg                                                                                                                       <  1:1213755/148‑1 (MQ=255)
                              cgcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGt                                                                                                                  <  1:2935957/148‑1 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTc                                                                                                                 <  1:2398794/148‑1 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTc                                                                                                                 <  2:661371/148‑1 (MQ=255)
                                  aaTTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTg                                                                                                              <  2:2199021/148‑1 (MQ=255)
                                    ttGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGtt                                                                                                            >  1:887338/1‑148 (MQ=255)
                                     tGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGttt                                                                                                           >  1:1116420/1‑148 (MQ=255)
                                      ggCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTa                                                                                                          <  1:831253/148‑1 (MQ=255)
                                         gATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCa                                                                                                       >  2:82716/1‑148 (MQ=255)
                                         gATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCa                                                                                                       <  2:838108/148‑1 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAAc                                                                                                     >  1:481972/1‑148 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAAc                                                                                                     <  2:3025706/148‑1 (MQ=255)
                                            gAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACt                                                                                                    <  1:899921/148‑1 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                <  1:893431/148‑1 (MQ=255)
                                                     gACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGtt                                                                                           <  2:1347669/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          <  2:1590074/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCACGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTGAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          >  1:73594/1‑148 (MQ=255)
                                                         gCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgt                                                                                       >  2:3235332/1‑148 (MQ=255)
                                                          cATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgtg                                                                                      >  1:2909348/1‑148 (MQ=255)
                                                          cATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGGTAtgtg                                                                                      >  2:2044448/1‑148 (MQ=255)
                                                           aTTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTgg                                                                                     >  1:1964599/1‑148 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCTGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    <  2:1626409/148‑1 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  1:2964186/1‑148 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  1:978269/1‑148 (MQ=255)
                                                              tATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATg                                                                                  >  1:2933285/1‑148 (MQ=255)
                                                              tATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTACCTCGCACGGTTTCGCCATTATAACGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACAACACACGTTATGTGGATg                                                                                  >  1:1289435/1‑148 (MQ=255)
                                                               atcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                 <  2:546892/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                <  1:1648371/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                <  2:2825232/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                <  2:2430597/148‑1 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               >  2:1719359/1‑148 (MQ=255)
                                                                  atcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAtt                                                                              >  1:2732589/1‑148 (MQ=255)
                                                                    ccAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcg                                                                            >  1:2786171/1‑148 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          >  2:549483/1‑148 (MQ=255)
                                                                       aCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttc                                                                         >  2:2622999/1‑148 (MQ=255)
                                                                       aCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAGGTGGATGAAttcgttc                                                                         >  2:3304012/1‑148 (MQ=255)
                                                                        cTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttcg                                                                        <  2:576384/148‑1 (MQ=255)
                                                                           aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTg                                                                     <  1:1320039/148‑1 (MQ=255)
                                                                             ttCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                   >  1:384354/1‑148 (MQ=255)
                                                                               ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGa                                                                 <  1:429598/148‑1 (MQ=255)
                                                                                 ccccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATg                                                               <  2:1679483/148‑1 (MQ=255)
                                                                                  cccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGc                                                              >  1:3111879/1‑148 (MQ=255)
                                                                                        agcGCATGGTGATGCGCGCCGCGTCGATTTTTCCCTCGCCCGGTTGCGCCATTATACAGGGACGCCAGTTGAACATTTTCAGCCGGTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                        <  2:3123774/146‑1 (MQ=255)
                                                                                        aTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                        >  2:1830680/1‑148 (MQ=255)
                                                                                        aTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                        >  1:3002396/1‑148 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGa                                                       <  1:2545544/148‑1 (MQ=255)
                                                                                            cATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTGGTTGGGGAGGCAGCCCGATcc                                                    >  2:1774570/1‑148 (MQ=255)
                                                                                            cATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATcc                                                    <  2:1014775/148‑1 (MQ=255)
                                                                                              ttttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTc                                                  >  1:1794321/1‑148 (MQ=255)
                                                                                                ttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGa                                                <  2:372004/148‑1 (MQ=255)
                                                                                                ttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGa                                                <  2:298916/148‑1 (MQ=255)
                                                                                                  gATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATc                                              <  2:2495068/148‑1 (MQ=255)
                                                                                                      cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  1:250766/148‑1 (MQ=255)
                                                                                                      cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  1:1920279/148‑1 (MQ=255)
                                                                                                       gcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGAt                                         <  2:630100/148‑1 (MQ=255)
                                                                                                        cgccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                        <  1:2666263/148‑1 (MQ=255)
                                                                                                         gccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAg                                       >  1:3285366/1‑148 (MQ=255)
                                                                                                           cgcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTc                                     <  1:3235332/148‑1 (MQ=255)
                                                                                                            gcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTcc                                    <  2:2732589/148‑1 (MQ=255)
                                                                                                             cgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACAACACACGGTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTccc                                   >  1:998964/1‑148 (MQ=255)
                                                                                                              gTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCt                                  <  2:2875152/148‑1 (MQ=255)
                                                                                                              gTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCt                                  <  2:1596393/148‑1 (MQ=255)
                                                                                                                cGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                <  2:2159584/148‑1 (MQ=255)
                                                                                                                 gATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACa                               <  1:2110227/148‑1 (MQ=255)
                                                                                                                   tttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                             >  1:766769/1‑148 (MQ=255)
                                                                                                                      ttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                          <  1:2158403/148‑1 (MQ=255)
                                                                                                                      ttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                          <  1:491732/148‑1 (MQ=255)
                                                                                                                       tCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGccc                         >  2:267616/1‑148 (MQ=255)
                                                                                                                           tCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTc                     >  2:2002121/1‑148 (MQ=255)
                                                                                                                             gCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCtt                   <  1:2110887/148‑1 (MQ=255)
                                                                                                                             gCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCtt                   <  2:1660520/148‑1 (MQ=255)
                                                                                                                              cACGGTTGCGCCATTATACAGGTACGACAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtg                  <  1:345940/148‑1 (MQ=255)
                                                                                                                                cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgtg                >  2:2893094/1‑148 (MQ=255)
                                                                                                                                cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgtg                >  2:1242422/1‑148 (MQ=255)
                                                                                                                                           ttATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAAc     >  1:287465/1‑148 (MQ=255)
                                                                                                                                              taCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg  <  1:2287169/148‑1 (MQ=255)
                                                                                                                                              taCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg  <  1:626149/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑