Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,051,691 G→T 11.9% S494* (TCG→TAG)  yeeO ← putative multdrug exporter, MATE family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,051,6910GT11.9% 519.3 / 32.4 157S494* (TCG→TAG) yeeOputative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand):  ref base G (67/71);  new base T (12/7);  total (79/78)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

ATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGC  >  CP009273/2051545‑2051838
                                                                                                                                                  |                                                                                                                                                   
aTTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGa                                                                                                                                                    >  1:2438575/1‑148 (MQ=255)
 ttAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGac                                                                                                                                                   >  2:1281402/1‑148 (MQ=255)
     cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                               >  1:1244544/1‑148 (MQ=255)
     cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                               >  1:2865531/1‑148 (MQ=255)
     cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                               <  2:431761/148‑1 (MQ=255)
        tCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTg                                                                                                                                            >  1:1147515/1‑148 (MQ=255)
        tCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTg                                                                                                                                            >  1:3303532/1‑148 (MQ=255)
        tCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTg                                                                                                                                            >  1:318378/1‑148 (MQ=255)
           gccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCt                                                                                                                                         <  2:3076606/148‑1 (MQ=255)
            ccgTTCTACCGACTGACCTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtt                                                                                                                                        <  2:1673286/148‑1 (MQ=255)
            ccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTAGCtt                                                                                                                                        <  1:1939640/148‑1 (MQ=255)
               ttCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttt                                                                                                                                     <  1:2779132/148‑1 (MQ=255)
                tCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtttttt                                                                                                                                    >  1:1984607/1‑148 (MQ=255)
                 cTAACGACTGAACTACAGATGAATCGTGAGAACGAGTCGAATATTAGCGATGCCCACCCACTATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATATGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt                                                                                                                                   <  1:870099/148‑1 (MQ=255)
                   aCCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTca                                                                                                                                 >  2:2667542/1‑148 (MQ=255)
                     cGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTcaca                                                                                                                               <  1:2433564/148‑1 (MQ=255)
                       aCTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCACAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACt                                                                                                                             >  2:3102220/1‑148 (MQ=255)
                       aCTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACt                                                                                                                             <  2:1424070/148‑1 (MQ=255)
                            aCTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGc                                                                                                                        >  2:2598750/1‑148 (MQ=255)
                                     gAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGt                                                                                                               >  2:1625844/1‑148 (MQ=255)
                                       aTCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCg                                                                                                             >  2:417132/1‑148 (MQ=255)
                                        tCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGCCGa                                                                                                            <  2:518017/148‑1 (MQ=255)
                                           tGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAggg                                                                                                         >  1:1472988/1‑148 (MQ=255)
                                            gagaACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGt                                                                                                        <  1:2759854/148‑1 (MQ=255)
                                            gagaACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGt                                                                                                        <  1:3078060/148‑1 (MQ=255)
                                                 cGAGGCGAATATTAGCGATGCCCACCCACACTGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTc                                                                                                   <  2:828067/148‑1 (MQ=255)
                                                 cGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTc                                                                                                   <  2:3177136/148‑1 (MQ=255)
                                                 cGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTc                                                                                                   >  2:2608849/1‑148 (MQ=255)
                                                  gAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTcc                                                                                                  <  1:2231007/148‑1 (MQ=255)
                                                    ggCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCAt                                                                                                <  1:1684170/148‑1 (MQ=255)
                                                      cGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAg                                                                                              <  1:1148626/148‑1 (MQ=255)
                                                      cGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAg                                                                                              <  1:333460/148‑1 (MQ=255)
                                                       gAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGc                                                                                             <  1:103856/148‑1 (MQ=255)
                                                       gAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGc                                                                                             <  1:103468/148‑1 (MQ=255)
                                                         atatTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCa                                                                                           <  2:760121/148‑1 (MQ=255)
                                                          tatTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCaa                                                                                          >  2:217384/1‑148 (MQ=255)
                                                           atTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAAc                                                                                         >  1:1163555/1‑148 (MQ=255)
                                                             tAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGt                                                                                       <  2:2058192/148‑1 (MQ=255)
                                                             tAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGt                                                                                       >  1:1266824/1‑148 (MQ=255)
                                                              aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                      <  2:528211/148‑1 (MQ=255)
                                                              aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                      <  2:1079837/148‑1 (MQ=255)
                                                              aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                      <  2:1079785/148‑1 (MQ=255)
                                                                cGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCa                                                                                    >  1:3104122/1‑148 (MQ=255)
                                                                cGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAACTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCa                                                                                    >  1:3104134/1‑148 (MQ=255)
                                                                  aTGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGt                                                                                  >  2:394704/1‑148 (MQ=255)
                                                                   tGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTa                                                                                 <  2:1023847/148‑1 (MQ=255)
                                                                    gCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTaa                                                                                <  2:1831837/148‑1 (MQ=255)
                                                                    gCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTaa                                                                                >  2:2012635/1‑148 (MQ=255)
                                                                         cccacAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCAtt                                                                           <  2:790225/148‑1 (MQ=255)
                                                                           cacaATGTCAAATCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCg                                                                         >  1:550906/1‑148 (MQ=255)
                                                                           cacaATGTCAAATCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCg                                                                         >  1:2298362/1‑148 (MQ=255)
                                                                             caATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGcc                                                                       >  2:2569459/1‑148 (MQ=255)
                                                                             caATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGcc                                                                       <  1:2137147/148‑1 (MQ=255)
                                                                               aTGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAg                                                                     >  2:1424661/1‑148 (MQ=255)
                                                                                tGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGt                                                                    >  1:1938528/1‑148 (MQ=255)
                                                                                tGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTAACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCAATCGCCAGt                                                                    >  1:1260958/1‑148 (MQ=255)
                                                                                 gTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTa                                                                   >  2:686651/1‑148 (MQ=255)
                                                                                   cAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaa                                                                 <  2:3100876/148‑1 (MQ=255)
                                                                                    aaaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaaa                                                                >  1:942814/1‑148 (MQ=255)
                                                                                    aaaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaaa                                                                <  1:788167/148‑1 (MQ=255)
                                                                                    aaaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaaa                                                                >  1:942779/1‑148 (MQ=255)
                                                                                    aaaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaaa                                                                >  1:2768874/1‑148 (MQ=255)
                                                                                       gCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAg                                                             >  2:728939/1‑148 (MQ=255)
                                                                                         cTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCa                                                           <  2:2806477/148‑1 (MQ=255)
                                                                                         cTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCa                                                           <  2:2807614/148‑1 (MQ=255)
                                                                                            ttttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACgg                                                        <  1:450777/148‑1 (MQ=255)
                                                                                            ttttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACgg                                                        >  1:1415557/1‑148 (MQ=255)
                                                                                             tttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGc                                                       <  2:2825018/148‑1 (MQ=255)
                                                                                                   aaTTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGcac                                                 <  2:2370433/148‑1 (MQ=255)
                                                                                                     tttGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAg                                               >  2:2559981/1‑148 (MQ=255)
                                                                                                      ttGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGc                                              >  1:1660989/1‑148 (MQ=255)
                                                                                                      ttGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGc                                              >  2:3171200/1‑148 (MQ=255)
                                                                                                         aaaTCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCa                                           >  2:101369/1‑148 (MQ=255)
                                                                                                              gTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCgg                                      <  2:727266/148‑1 (MQ=255)
                                                                                                                   cTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAAc                                 <  1:101369/148‑1 (MQ=255)
                                                                                                                     gAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACAt                               <  2:1712033/148‑1 (MQ=255)
                                                                                                                     gAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACAt                               <  1:17928/148‑1 (MQ=255)
                                                                                                                      aaaTAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACAtt                              <  1:1019403/148‑1 (MQ=255)
                                                                                                                      aaaTAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACAtt                              >  2:2724230/1‑148 (MQ=255)
                                                                                                                        ataatCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTcc                            >  2:2192495/1‑148 (MQ=255)
                                                                                                                        ataatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCAGCACAGCCCAGTCGGCAAACATTcc                            <  1:3041828/148‑1 (MQ=255)
                                                                                                                         taatCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                           >  2:26469/1‑148 (MQ=255)
                                                                                                                         taatCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                           >  1:2587435/1‑148 (MQ=255)
                                                                                                                           atCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCAt                         <  2:1492949/148‑1 (MQ=255)
                                                                                                                            tCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATc                        >  1:485292/1‑148 (MQ=255)
                                                                                                                            tCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATc                        >  1:485429/1‑148 (MQ=255)
                                                                                                                               gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCCTCCAg                     >  1:2326017/1‑148 (MQ=255)
                                                                                                                               gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAg                     >  2:2424432/1‑148 (MQ=255)
                                                                                                                               gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAg                     <  1:2337396/148‑1 (MQ=255)
                                                                                                                               gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAg                     >  1:2192979/1‑148 (MQ=255)
                                                                                                                                  ttttGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGaca                  <  2:3303532/148‑1 (MQ=255)
                                                                                                                                  ttttGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGaca                  >  2:1218677/1‑148 (MQ=255)
                                                                                                                                  ttttGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGaca                  >  2:1218849/1‑148 (MQ=255)
                                                                                                                                  ttttGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGaca                  >  2:2325456/1‑148 (MQ=255)
                                                                                                                                       tCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACaccaac             <  2:335899/148‑1 (MQ=255)
                                                                                                                                         gTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACaccaacca           <  1:388295/148‑1 (MQ=255)
                                                                                                                                          tttATTCCGACACAACTGGCTTTTTTTCACACTTTTGAGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCcaccac          >  1:96626/1‑148 (MQ=255)
                                                                                                                                           ttATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACcaca         >  1:1480088/1‑148 (MQ=255)
                                                                                                                                              ttCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACAccc      <  2:2910482/148‑1 (MQ=255)
                                                                                                                                              ttCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACAccc      >  1:2134426/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGC  >  CP009273/2051545‑2051838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑