Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,086,004 C→A 85.6% A42A (GCC→GCA)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,086,0040CA85.6% 333.0 / 60.4 151A42A (GCC→GCA) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (10/12);  new base A (60/69);  total (70/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 151 total aligned reads displayed.

CCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085863‑2086148
                                                                                                                                             |                                                                                                                                                
cccTTAAGGAGCAAGCATGAGCACCGTGACTATTACCTTTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                            <  2:17099/148‑1 (MQ=255)
   ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         <  2:77956/148‑1 (MQ=255)
   ttAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGttt                                                                                                                                         <  2:77925/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:2644629/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        >  2:3160762/1‑148 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:2146396/148‑1 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        >  2:1863470/1‑148 (MQ=255)
    tAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                        <  2:397700/148‑1 (MQ=255)
     aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCa                                                                                                                                       >  2:1659998/1‑148 (MQ=255)
     aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCa                                                                                                                                       >  2:1659997/1‑148 (MQ=255)
      aGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAACACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAg                                                                                                                                      <  2:2822650/148‑1 (MQ=255)
      aGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAg                                                                                                                                      <  2:2822655/148‑1 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    <  1:2768482/148‑1 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    >  2:2498039/1‑148 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    >  2:2497887/1‑148 (MQ=255)
        gAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCt                                                                                                                                    <  1:2257884/148‑1 (MQ=255)
         agcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCtt                                                                                                                                   <  2:2369593/148‑1 (MQ=255)
          gcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTa                                                                                                                                  >  1:3246066/1‑148 (MQ=255)
           caagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTAc                                                                                                                                 >  1:2693972/1‑148 (MQ=255)
              gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCa                                                                                                                              >  1:2583388/1‑148 (MQ=255)
              gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCa                                                                                                                              >  2:1683137/1‑148 (MQ=255)
               caTGAGCACCGAGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGAGCGTCGTCTGGGCGGTAACGGCTATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAg                                                                                                                             >  2:2086846/1‑148 (MQ=255)
                 tGAGCACCGTGACTATTCCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCa                                                                                                                           <  2:573092/148‑1 (MQ=255)
                        cGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTc                                                                                                                    >  1:1304020/1‑148 (MQ=255)
                          tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCaa                                                                                                                  <  1:1090890/148‑1 (MQ=255)
                          tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCaa                                                                                                                  <  1:1092342/148‑1 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:1165729/1‑148 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:1520443/1‑148 (MQ=255)
                           gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAc                                                                                                                 >  2:817740/1‑148 (MQ=255)
                               aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCt                                                                                                             >  2:2734073/1‑148 (MQ=255)
                               aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCt                                                                                                             <  2:2901632/148‑1 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  1:2880976/1‑148 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  2:1938031/1‑148 (MQ=255)
                                 tACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                           >  2:2192633/1‑148 (MQ=255)
                                   ccGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAccc                                                                                                         >  2:1119843/1‑148 (MQ=255)
                                   ccGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAccc                                                                                                         >  2:1545190/1‑148 (MQ=255)
                                     gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       <  1:2941047/148‑1 (MQ=255)
                                     gATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCgg                                                                                                       <  1:3217394/148‑1 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     >  1:1019594/1‑148 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     >  2:1805468/1‑148 (MQ=255)
                                       tttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGaa                                                                                                     <  2:357369/148‑1 (MQ=255)
                                        ttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                    >  2:2068726/1‑148 (MQ=255)
                                           gcgcGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                 <  2:2717568/148‑1 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         <  2:938100/148‑1 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         >  1:862166/1‑148 (MQ=255)
                                                   aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                         >  1:2967103/1‑148 (MQ=255)
                                                    aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                        >  2:2534195/1‑148 (MQ=255)
                                                         ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCAACTGCAGTGGAGTTTCAGCTTCCTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   >  2:1700999/1‑148 (MQ=255)
                                                         ccGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCAACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                   >  2:3189798/1‑148 (MQ=255)
                                                          cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                  <  2:2904081/148‑1 (MQ=255)
                                                          cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                  <  2:1508989/148‑1 (MQ=255)
                                                           gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAt                                                                                 <  2:2362974/148‑1 (MQ=255)
                                                           gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAt                                                                                 <  1:2534195/148‑1 (MQ=255)
                                                              aCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGa                                                                              <  1:274867/148‑1 (MQ=255)
                                                              aCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGa                                                                              <  1:274775/148‑1 (MQ=255)
                                                                 tGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaaa                                                                           >  2:3170354/1‑148 (MQ=255)
                                                                     gCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAc                                                                       >  1:2246290/1‑148 (MQ=255)
                                                                      ccGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcg                                                                      <  1:606916/148‑1 (MQ=255)
                                                                      ccGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcg                                                                      <  1:1742429/148‑1 (MQ=255)
                                                                        gTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAcgcg                                                                    <  1:730450/148‑1 (MQ=255)
                                                                          aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  2:227899/148‑1 (MQ=255)
                                                                          aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                  <  2:33089/148‑1 (MQ=255)
                                                                            cAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                >  2:1053218/1‑148 (MQ=255)
                                                                                     cgtcgtCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCg                                                       >  1:807518/1‑148 (MQ=255)
                                                                                         gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                   <  1:2455459/148‑1 (MQ=255)
                                                                                         gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                   <  1:796597/148‑1 (MQ=255)
                                                                                          tcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaaa                                                  >  2:1477695/1‑148 (MQ=255)
                                                                                            tGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAcc                                                >  1:2674400/1‑148 (MQ=255)
                                                                                            tGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAcc                                                >  1:1525194/1‑148 (MQ=255)
                                                                                             gggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCg                                               >  1:1059035/1‑148 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              >  1:2388933/1‑148 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  1:1165729/148‑1 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  2:341982/148‑1 (MQ=255)
                                                                                              ggCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                              <  2:341798/148‑1 (MQ=255)
                                                                                                  gTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCa                                          <  1:431032/148‑1 (MQ=255)
                                                                                                  gTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCa                                          <  1:431011/148‑1 (MQ=255)
                                                                                                   tAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                         <  2:3222607/148‑1 (MQ=255)
                                                                                                    aaCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAgg                                        <  1:1998558/148‑1 (MQ=255)
                                                                                                     aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGt                                       >  1:1863377/1‑148 (MQ=255)
                                                                                                      cGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                      >  2:1718793/1‑148 (MQ=255)
                                                                                                         cGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTg                                   >  1:84827/1‑148 (MQ=255)
                                                                                                            tGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTc                                <  1:577733/148‑1 (MQ=255)
                                                                                                                 ggCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCg                           <  2:1402780/148‑1 (MQ=255)
                                                                                                                  gCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGt                          >  1:804486/1‑148 (MQ=255)
                                                                                                                   cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTg                         >  2:831329/1‑148 (MQ=255)
                                                                                                                         gCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCgg                   <  2:185591/148‑1 (MQ=255)
                                                                                                                              cGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGaa              >  1:1945405/1‑148 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:2291612/148‑1 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           >  1:3177186/1‑148 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:1940008/148‑1 (MQ=255)
                                                                                                                                 aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGNGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt           <  1:1683137/148‑1 (MQ=255)
                                                                                                                                  tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa          <  2:2951238/148‑1 (MQ=255)
                                                                                                                                  tACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa          <  2:2951234/148‑1 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      >  1:3024102/1‑148 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      >  1:3027169/1‑148 (MQ=255)
                                                                                                                                      ccACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGa      <  1:1805468/148‑1 (MQ=255)
                                                                                                                                       cACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGaa     >  1:1398673/1‑148 (MQ=255)
                                                                                                                                        aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAACGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    <  2:230122/148‑1 (MQ=255)
                                                                                                                                        aCTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAc    >  1:1983584/1‑148 (MQ=255)
                                                                                                                                          tGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAActg  >  1:718135/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTG  >  CP009273/2085863‑2086148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑