Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,099,730 A→T 8.5% C326* (TGT→TGA)  wbbH ← O‑antigen polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,099,7300AT 8.5% 686.4 / 43.0 223C326* (TGT→TGA) wbbHO‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (121/83);  new base T (12/7);  total (133/90)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

ATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCAT  >  CP009273/2099584‑2099876
                                                                                                                                                  |                                                                                                                                                  
aTACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATAc                                                                                                                                                   <  1:945662/148‑1 (MQ=255)
 tACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCa                                                                                                                                                  <  2:2606230/148‑1 (MQ=255)
  aCAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCAg                                                                                                                                                 >  1:217653/1‑148 (MQ=255)
  aCAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAg                                                                                                                                                 >  1:3005877/1‑148 (MQ=255)
   cAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGc                                                                                                                                                >  2:1437576/1‑148 (MQ=255)
         gTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGaa                                                                                                                                          <  2:1016772/148‑1 (MQ=255)
         gTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGaa                                                                                                                                          >  2:1678159/1‑148 (MQ=255)
         gTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGaa                                                                                                                                          >  2:1882651/1‑148 (MQ=255)
           tATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATa                                                                                                                                        >  1:974155/1‑148 (MQ=255)
                   tCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACcatcat                                                                                                                                >  1:3205080/1‑148 (MQ=255)
                     cAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCAtcatca                                                                                                                              >  1:971129/1‑148 (MQ=255)
                          gCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAAtag                                                                                                                         >  2:2682004/1‑148 (MQ=255)
                          gCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAAtag                                                                                                                         >  1:1356053/1‑148 (MQ=255)
                           cTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAAtagc                                                                                                                        <  1:1636150/148‑1 (MQ=255)
                            tAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAAtagct                                                                                                                       >  2:1522395/1‑148 (MQ=255)
                             aaTATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAAtagctt                                                                                                                      >  2:2056045/1‑148 (MQ=255)
                             aaTATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAAtagctt                                                                                                                      >  1:1466054/1‑148 (MQ=255)
                                  tAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTc                                                                                                                 >  1:788180/1‑148 (MQ=255)
                                      cATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCCTGAATAACCATCATCAAATAGCTTAGCTCcgcg                                                                                                             >  2:2378492/1‑148 (MQ=255)
                                        tGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGa                                                                                                           >  1:2592337/1‑148 (MQ=255)
                                        tGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATAAAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGa                                                                                                           >  1:608964/1‑148 (MQ=255)
                                         gAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGaa                                                                                                          <  1:712429/148‑1 (MQ=255)
                                            gCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAata                                                                                                       <  1:2970252/148‑1 (MQ=255)
                                             cTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAatat                                                                                                      <  2:1183068/148‑1 (MQ=255)
                                             cTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAatat                                                                                                      <  1:2979103/148‑1 (MQ=255)
                                              tttCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGACAtata                                                                                                     >  2:3014212/1‑148 (MQ=255)
                                              tttCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAAtata                                                                                                     <  1:410203/148‑1 (MQ=255)
                                               ttCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATaa                                                                                                    >  1:1332958/1‑148 (MQ=255)
                                                 cATGATAAAAAATGAAAGAAAAGTTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAAc                                                                                                  <  2:1755047/148‑1 (MQ=255)
                                                    gATAAAAAATGAAAGAAAAGTTATAAATAAAATATGAATAAAATATTTTCACATATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATa                                                                                               <  2:1840365/148‑1 (MQ=255)
                                                    gATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATa                                                                                               >  2:1267439/1‑148 (MQ=255)
                                                     aTAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATaa                                                                                              <  2:3005877/148‑1 (MQ=255)
                                                     aTAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATaa                                                                                              <  1:1242/148‑1 (MQ=255)
                                                     aTAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATaa                                                                                              <  1:2181821/148‑1 (MQ=255)
                                                      tAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAAt                                                                                             <  2:2766168/148‑1 (MQ=255)
                                                       aaaaaaTGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATc                                                                                            <  1:3106194/148‑1 (MQ=255)
                                                       aaaaaaTGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATc                                                                                            >  2:858738/1‑148 (MQ=255)
                                                       aaaaaaTGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGACATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATc                                                                                            >  1:8775/1‑148 (MQ=255)
                                                            aTGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGaaa                                                                                       <  2:2666830/148‑1 (MQ=255)
                                                             tGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAACTATAAACATAATCCGaaaa                                                                                      >  1:1784144/1‑148 (MQ=255)
                                                             tGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGaaaa                                                                                      >  2:3091990/1‑148 (MQ=255)
                                                              gaaagaaaATGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGaaaaa                                                                                     <  1:2025633/148‑1 (MQ=255)
                                                              gaaagaaaAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGaaaaa                                                                                     >  2:3142476/1‑148 (MQ=255)
                                                                   aaaaGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGt                                                                                <  2:2838362/148‑1 (MQ=255)
                                                                           taaataaaATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACAt                                                                        >  1:3245184/1‑148 (MQ=255)
                                                                             aataaaataTGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGACATATAAACATAATCCGAAAAAGCAGTATAAACAttt                                                                      >  1:2524361/1‑148 (MQ=255)
                                                                               taaaataTGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATttgt                                                                    <  2:2203349/148‑1 (MQ=255)
                                                                                aaaataTGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATttgtt                                                                   >  1:107605/1‑148 (MQ=255)
                                                                                  aataTGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTgg                                                                 >  1:2306022/1‑148 (MQ=255)
                                                                                   atatGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGc                                                                >  2:3039220/1‑148 (MQ=255)
                                                                                     atGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCaa                                                              <  1:2253409/148‑1 (MQ=255)
                                                                                      tGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCaaa                                                             >  2:2150962/1‑148 (MQ=255)
                                                                                        aaTAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAacc                                                           <  1:327414/148‑1 (MQ=255)
                                                                                          tAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAATCATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAaccca                                                         >  1:380437/1‑148 (MQ=255)
                                                                                          tAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAaccca                                                         >  2:340453/1‑148 (MQ=255)
                                                                                          tAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAaccca                                                         >  1:757571/1‑148 (MQ=255)
                                                                                          tAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAaccca                                                         >  1:2004961/1‑148 (MQ=255)
                                                                                          tAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAaccca                                                         >  2:1592736/1‑148 (MQ=255)
                                                                                             aaTATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAacccaccc                                                      >  2:1277596/1‑148 (MQ=255)
                                                                                             aaTATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAacccaccc                                                      >  1:1923431/1‑148 (MQ=255)
                                                                                              atatTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAAcccaccca                                                     >  1:232702/1‑148 (MQ=255)
                                                                                              atatTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAAcccaccca                                                     >  1:1711271/1‑148 (MQ=255)
                                                                                               tatTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACccacccac                                                    >  1:496539/1‑148 (MQ=255)
                                                                                                atTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGGAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcaca                                                   >  1:1695725/1‑148 (MQ=255)
                                                                                                  tttCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcacaca                                                 >  2:3145305/1‑148 (MQ=255)
                                                                                                  tttCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcacaca                                                 >  2:2660605/1‑148 (MQ=255)
                                                                                                  tttCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcacaca                                                 >  2:2248162/1‑148 (MQ=255)
                                                                                                  tttCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcacaca                                                 >  2:612693/1‑148 (MQ=255)
                                                                                                  tttCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCcacaca                                                 >  1:865094/1‑148 (MQ=255)
                                                                                                     cacaGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAAt                                              <  2:1703930/148‑1 (MQ=255)
                                                                                                      acaGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAAtt                                             >  1:1383405/1‑148 (MQ=255)
                                                                                                       caGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTc                                            >  2:313038/1‑148 (MQ=255)
                                                                                                       caGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTc                                            >  2:436844/1‑148 (MQ=255)
                                                                                                       caGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTc                                            >  2:2101595/1‑148 (MQ=255)
                                                                                                       caGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTc                                            <  1:136238/148‑1 (MQ=255)
                                                                                                         gATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCtt                                          <  2:1219252/148‑1 (MQ=255)
                                                                                                          atatGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCttt                                         >  2:3207006/1‑148 (MQ=255)
                                                                                                             tGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATg                                      >  1:2396767/1‑148 (MQ=255)
                                                                                                                 aTTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCacc                                  >  2:363147/1‑146 (MQ=255)
                                                                                                                  tttCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACg                                 <  2:2396767/148‑1 (MQ=255)
                                                                                                                   ttCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGa                                >  1:3011378/1‑148 (MQ=255)
                                                                                                                     cGAGACAATCTCCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACa                              >  1:518958/1‑148 (MQ=255)
                                                                                                                      gagaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACAt                             <  2:3189277/148‑1 (MQ=255)
                                                                                                                       agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATa                            <  2:2942099/148‑1 (MQ=255)
                                                                                                                       agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATa                            <  2:765320/148‑1 (MQ=255)
                                                                                                                         aCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATaga                          >  1:146133/1‑148 (MQ=255)
                                                                                                                         aCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATaga                          >  1:2243325/1‑148 (MQ=255)
                                                                                                                          cAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATagag                         <  2:2500641/148‑1 (MQ=255)
                                                                                                                          cAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATagag                         <  2:2987412/148‑1 (MQ=255)
                                                                                                                           aaTCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAgaga                        <  1:3131013/148‑1 (MQ=255)
                                                                                                                              ctcCATAAAACACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTc                     <  2:2815966/148‑1 (MQ=255)
                                                                                                                                    aaaaCACCTGAAATTCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTg               >  2:3003592/1‑148 (MQ=255)
                                                                                                                                    aaaaCACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTg               <  1:2737630/148‑1 (MQ=255)
                                                                                                                                        cacCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTaa           <  2:2306022/148‑1 (MQ=255)
                                                                                                                                        cacCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTaa           <  2:1923431/148‑1 (MQ=255)
                                                                                                                                         acCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAAt          <  1:1578413/148‑1 (MQ=255)
                                                                                                                                             gAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGcc      <  1:1734833/148‑1 (MQ=255)
                                                                                                                                              aaaTACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGccc     >  2:2033813/1‑148 (MQ=255)
                                                                                                                                                 tACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCAt  <  1:1282356/148‑1 (MQ=255)
                                                                                                                                                 tACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCAt  >  1:2431146/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCAT  >  CP009273/2099584‑2099876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑