BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,220,503	C→T	12.2%	V83M (GTG→ATG)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,220,503	0	C	T	12.2%	459.8 / 41.3	148	V83M (GTG→ATG)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (63/67); new base T (10/8); total (73/75)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 153 total aligned reads displayed.

CTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTG  >  CP009273/2220357‑2220650

 tgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACAt                                                                                                                                                   <  1:2445849/148‑1 (MQ=255)

  gccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATc                                                                                                                                                  >  1:163292/1‑148 (MQ=255)

   ccgttGTTTCACAATCTGCCGAGCCGCATTTTGCTAGCATAAGAATGCACAATCGACATCAACGTTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCa                                                                                                                                                 <  1:945413/145‑1 (MQ=255)

   ccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCACCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCa                                                                                                                                                 <  1:945517/148‑1 (MQ=255)

    cctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCaa                                                                                                                                                >  2:3100835/1‑148 (MQ=255)

      ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATCTCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATg                                                                                                                                              >  2:1968113/1‑148 (MQ=255)

      ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATg                                                                                                                                              >  2:2314497/1‑148 (MQ=255)

      ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATg                                                                                                                                              >  2:2314491/1‑148 (MQ=255)

      ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATg                                                                                                                                              >  2:1968110/1‑148 (MQ=255)

       tGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATgc                                                                                                                                             >  2:1599424/1‑148 (MQ=255)

         tttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATgcgc                                                                                                                                           >  2:1070658/1‑148 (MQ=255)

         tttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATgcgc                                                                                                                                           >  1:1464048/1‑148 (MQ=255)

           tCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGTTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGccc                                                                                                                                         <  1:3193531/148‑1 (MQ=255)

           tCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGccc                                                                                                                                         <  2:1048112/148‑1 (MQ=255)

                 tCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCg                                                                                                                                   >  1:2841228/1‑148 (MQ=255)

                 tCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCg                                                                                                                                   <  2:3129354/148‑1 (MQ=255)

                   tGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAACGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGtt                                                                                                                                 <  1:1078579/148‑1 (MQ=255)

                   tGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGtt                                                                                                                                 <  1:2732021/148‑1 (MQ=255)

                     aCGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGa                                                                                                                               <  2:1617992/148‑1 (MQ=255)

                       gAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAAt                                                                                                                             >  1:3260785/1‑148 (MQ=255)

                          ccGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAg                                                                                                                          >  2:1437169/1‑148 (MQ=255)

                           cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         <  2:1548683/148‑1 (MQ=255)

                           cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         <  2:3171976/148‑1 (MQ=255)

                           cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCAAATCAACAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         <  1:1468722/148‑1 (MQ=255)

                            gCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGtt                                                                                                                        >  2:1152547/1‑148 (MQ=255)

                               aTTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGACTGAGTTtct                                                                                                                     >  2:2486883/1‑148 (MQ=255)

                               aTTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     <  2:2357754/148‑1 (MQ=255)

                               aTTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     <  2:1456253/148‑1 (MQ=255)

                               aTTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     >  1:2654762/1‑148 (MQ=255)

                                 ttGCGAGCATAAGAATGCACCATCGACATCAACGTTAAAAATCTAGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTcc                                                                                                                   <  2:2960357/148‑1 (MQ=255)

                                      aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAgcgc                                                                                                              <  1:2580893/148‑1 (MQ=255)

                                      aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAgcgc                                                                                                              <  1:1812643/148‑1 (MQ=255)

                                      aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAACCCATATCAACAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAgcgc                                                                                                              <  1:2580280/148‑1 (MQ=255)

                                           aaGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTg                                                                                                         >  1:1576922/1‑148 (MQ=255)

                                             gAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGcc                                                                                                       >  2:1332534/1‑148 (MQ=255)

                                              aaTGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGccc                                                                                                      >  1:1566334/1‑148 (MQ=255)

                                                 gCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCtt                                                                                                   <  2:1337939/148‑1 (MQ=255)

                                                  cACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTc                                                                                                  >  2:2554178/1‑148 (MQ=255)

                                                   aCCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCt                                                                                                 <  2:163292/148‑1 (MQ=255)

                                                    ccATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTg                                                                                                >  1:1161172/1‑148 (MQ=255)

                                                     cATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTgg                                                                                               <  2:6110/148‑1 (MQ=255)

                                                         gACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGa                                                                                           <  2:2284054/148‑1 (MQ=255)

                                                           cATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                         >  1:496652/1‑148 (MQ=255)

                                                            acaaaCGGTAAAAATCTTGCGCCCCACTTCAAAAGCAATCTCAACAACCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTg                                                                                        <  1:1544580/145‑1 (MQ=255)

                                                            aTCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTg                                                                                        <  1:1944398/148‑1 (MQ=255)

                                                            aTCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTg                                                                                        <  1:1546699/148‑1 (MQ=255)

                                                            aTCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTg                                                                                        <  2:1509925/148‑1 (MQ=255)

                                                                   gTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGAt                                                                                 <  2:174360/148‑1 (MQ=255)

                                                                    tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATc                                                                                <  1:2168407/148‑1 (MQ=255)

                                                                    tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATc                                                                                <  1:2168176/148‑1 (MQ=255)

                                                                    tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATc                                                                                <  1:1291052/148‑1 (MQ=255)

                                                                    tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATc                                                                                <  2:2088815/148‑1 (MQ=255)

                                                                    tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATc                                                                                >  2:1928114/1‑148 (MQ=255)

                                                                     aaaaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATcc                                                                               <  2:732118/148‑1 (MQ=255)

                                                                     aaaaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATcc                                                                               <  1:1319076/148‑1 (MQ=255)

                                                                       aaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAg                                                                             <  1:1070658/148‑1 (MQ=255)

                                                                                 ccACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtc                                                                   <  1:164233/148‑1 (MQ=255)

                                                                                  cACTCATCAAAAGCCTTATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtct                                                                  <  1:2813707/148‑1 (MQ=255)

                                                                                  cACTCATCAAAAGCAATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCGGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtct                                                                  <  1:2813631/148‑1 (MQ=255)

                                                                                   aCTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtctc                                                                 <  2:1244570/148‑1 (MQ=255)

                                                                                     tcatcaAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCg                                                               >  2:2849373/1‑148 (MQ=255)

                                                                                          aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGt                                                          <  1:2314497/148‑1 (MQ=255)

                                                                                          aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGt                                                          >  1:3169715/1‑148 (MQ=255)

                                                                                          aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGt                                                          <  1:2314496/148‑1 (MQ=255)

                                                                                           aaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTa                                                         >  2:11400/1‑148 (MQ=255)

                                                                                             aGCCATATCAAGAAACGGCGCTTTGGTCATTGCATCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACg                                                       >  1:1691546/1‑148 (MQ=255)

                                                                                             aGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACg                                                       >  2:769056/1‑148 (MQ=255)

                                                                                             aGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACg                                                       >  1:1691543/1‑148 (MQ=255)

                                                                                               ccATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGcc                                                     >  2:140988/1‑148 (MQ=255)

                                                                                               ccATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGcc                                                     >  1:2652669/1‑148 (MQ=255)

                                                                                                    tCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGc                                                >  1:380815/1‑148 (MQ=255)

                                                                                                      aaGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTa                                              >  1:240507/1‑148 (MQ=255)

                                                                                                       aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTaa                                             >  2:432714/1‑148 (MQ=255)

                                                                                                       aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTaa                                             <  2:1576922/148‑1 (MQ=255)

                                                                                                        gAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAAc                                            >  2:784169/1‑148 (MQ=255)

                                                                                                        gAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAAc                                            >  2:1379721/1‑148 (MQ=255)

                                                                                                        gAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAAc                                            >  1:645494/1‑148 (MQ=255)

                                                                                                          aaCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTa                                          <  2:414911/148‑1 (MQ=255)

                                                                                                           aCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTAc                                         >  2:3060273/1‑148 (MQ=255)

                                                                                                            cGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTAcc                                        >  2:2608220/1‑148 (MQ=255)

                                                                                                               cgcTTTGGTCATTGCACCCGCATTAGTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCa                                     <  1:2480126/148‑1 (MQ=255)

                                                                                                                  tttGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCAcgc                                  >  2:2059008/1‑148 (MQ=255)

                                                                                                                       tCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGt                             <  2:1566334/148‑1 (MQ=255)

                                                                                                                        cATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTa                            >  2:3131891/1‑148 (MQ=255)

                                                                                                                        cATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTa                            >  2:1069460/1‑148 (MQ=255)

                                                                                                                        cATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTa                            <  1:3055563/148‑1 (MQ=255)

                                                                                                                         aTTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTAt                           <  1:1950238/148‑1 (MQ=255)

                                                                                                                           tGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                         >  2:788022/1‑148 (MQ=255)

                                                                                                                           tGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                         >  1:1756845/1‑148 (MQ=255)

                                                                                                                           tGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                         <  2:1382533/148‑1 (MQ=255)

                                                                                                                              aCCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtttt                      <  1:3100835/148‑1 (MQ=255)

                                                                                                                               cccGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTg                     >  2:1304359/1‑148 (MQ=255)

                                                                                                                               cccGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTg                     <  1:2943749/148‑1 (MQ=255)

                                                                                                                               cccGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTg                     <  1:2941740/148‑1 (MQ=255)

                                                                                                                                 cGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGcc                   >  1:2862794/1‑148 (MQ=255)

                                                                                                                                     ttattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCctt               >  1:1150823/1‑148 (MQ=255)

                                                                                                                                     ttattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCctt               <  1:2533321/148‑1 (MQ=255)

                                                                                                                                               gCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGa     <  1:176421/148‑1 (MQ=255)

                                                                                                                                                cacaTCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAg    >  2:1790461/1‑148 (MQ=255)

                                                                                                                                                cacaTCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAg    >  2:433152/1‑148 (MQ=255)

CTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTG  >  CP009273/2220357‑2220650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`