Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,248,657 C→A 89.7% K3N (AAG→AAT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,248,6570CA89.7% 447.0 / 42.9 179K3N (AAG→AAT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (4/15);  new base A (87/73);  total (91/88)
Fisher's exact test for biased strand distribution p-value = 7.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAA  >  CP009273/2248512‑2248800
                                                                                                                                                 |                                                                                                                                               
tCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTAtt                                                                                                                                               >  1:3032129/1‑148 (MQ=255)
    gCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAGTCCAGAATAATTTTTCTATTTTcc                                                                                                                                           >  2:3181177/1‑148 (MQ=255)
     ccATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCa                                                                                                                                          >  2:1918486/1‑148 (MQ=255)
     ccATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCa                                                                                                                                          >  2:2786351/1‑148 (MQ=255)
     ccATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCa                                                                                                                                          >  2:2786193/1‑148 (MQ=255)
      cATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAt                                                                                                                                         <  1:294441/148‑1 (MQ=255)
       attaattaATTTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAtt                                                                                                                                        <  2:2815020/148‑1 (MQ=255)
        ttaattaaTGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTg                                                                                                                                       <  2:1108050/148‑1 (MQ=255)
            ttaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                   >  1:1871722/1‑148 (MQ=255)
             taatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCGTTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAt                                                                                                                                  >  1:825252/1‑148 (MQ=255)
             taatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAt                                                                                                                                  <  1:374134/148‑1 (MQ=255)
                tGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                               >  1:154124/1‑148 (MQ=255)
                 gTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTcc                                                                                                                              >  1:2909614/1‑148 (MQ=255)
                   tttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCctc                                                                                                                            >  1:2406317/1‑148 (MQ=255)
                   tttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            >  1:2849834/1‑148 (MQ=255)
                   tttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            <  2:2116034/148‑1 (MQ=255)
                   tttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            <  2:2116328/148‑1 (MQ=255)
                     tATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTg                                                                                                                          <  1:2756456/148‑1 (MQ=255)
                      aTCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGt                                                                                                                         <  1:1730534/148‑1 (MQ=255)
                      aTCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGt                                                                                                                         >  2:260268/1‑148 (MQ=255)
                        cAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGttt                                                                                                                       <  1:2998090/148‑1 (MQ=255)
                        cAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGttt                                                                                                                       <  1:2997905/148‑1 (MQ=255)
                          aGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTcc                                                                                                                     <  1:682457/148‑1 (MQ=255)
                           gCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCa                                                                                                                    >  1:2297065/1‑148 (MQ=255)
                            cGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAg                                                                                                                   >  1:1461019/1‑148 (MQ=255)
                             gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGt                                                                                                                  <  1:143379/148‑1 (MQ=255)
                               cTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTg                                                                                                                <  2:1460774/148‑1 (MQ=255)
                                       ccGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAttt                                                                                                        <  2:887663/148‑1 (MQ=255)
                                       ccGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAttt                                                                                                        >  2:187997/1‑148 (MQ=255)
                                            aCAATAGTGATTCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                   <  1:2586918/148‑1 (MQ=255)
                                            aCAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                   >  2:1522521/1‑148 (MQ=255)
                                            aCAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                   >  2:150501/1‑148 (MQ=255)
                                            aCAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                   <  1:2586587/148‑1 (MQ=255)
                                               aTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACa                                                                                                >  1:1244928/1‑148 (MQ=255)
                                                   tGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCaa                                                                                            >  2:3164754/1‑148 (MQ=255)
                                                    gATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCaaa                                                                                           >  1:570350/1‑148 (MQ=255)
                                                     aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAg                                                                                          <  1:1862476/148‑1 (MQ=255)
                                                     aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAg                                                                                          >  2:1627106/1‑148 (MQ=255)
                                                          taataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAt                                                                                     <  1:1486673/148‑1 (MQ=255)
                                                             taaATCTATTGCCGGATGTTTCGCCGCCATAATTATAGCAATAGCATCATCATGACACGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCTTTATTTTTTCTACATCAAAGAAAAttag                                                                                  >  2:2508877/1‑146 (MQ=255)
                                                              aaaTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGa                                                                                 <  2:2321865/148‑1 (MQ=255)
                                                              aaaTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGa                                                                                 >  1:660863/1‑148 (MQ=255)
                                                              aaaTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGa                                                                                 >  1:2321865/1‑148 (MQ=255)
                                                                  cTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCgg                                                                             >  1:905211/1‑148 (MQ=255)
                                                                    aTTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGc                                                                           >  1:928800/1‑148 (MQ=255)
                                                                    aTTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAACTCGACCGGGc                                                                           >  1:927047/1‑148 (MQ=255)
                                                                        ccGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATATTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTAAAGCAAATAAAATTCGACCGGGCCgat                                                                       >  1:1133263/1‑148 (MQ=255)
                                                                         cGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAAATTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatg                                                                      <  2:1312113/148‑1 (MQ=255)
                                                                          ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatga                                                                     >  2:286803/1‑148 (MQ=255)
                                                                          ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatga                                                                     >  2:2465377/1‑148 (MQ=255)
                                                                            aTGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                   >  2:2286287/1‑148 (MQ=255)
                                                                             tGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTg                                                                  >  1:1047217/1‑148 (MQ=255)
                                                                              gTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGa                                                                 >  2:1997208/1‑148 (MQ=255)
                                                                              gTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATGGTTTATTTCCTCTGTTTCCCGTTGAGTTATTTTTTCTACAGCAAATAAAATTCGACCGGGCCgctgtgtgt                                                                 >  1:2231465/1‑143 (MQ=255)
                                                                               tttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGaa                                                                <  2:1934111/148‑1 (MQ=255)
                                                                               tttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGaa                                                                <  2:1084574/148‑1 (MQ=255)
                                                                                  cgccgcCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCGGTTTCCAGTTGCTTTATTTTTTCTACAGAAAAGAAAATTCGACCGGGCCGATGATTGAATCt                                                             <  1:1140123/148‑1 (MQ=255)
                                                                                    ccgccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTa                                                           >  1:187415/1‑148 (MQ=255)
                                                                                              tataGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgt                                                 >  2:1570425/1‑148 (MQ=255)
                                                                                              tataGCAATAGCATCATCATGACACGGATCACAATCCAGAATAATTTTTCTATTTTCCATTTTTTATTTCCTCTTTTTCCAGTTGCTTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgt                                                 <  2:3129521/148‑1 (MQ=255)
                                                                                               atagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGACGATGATTGAATCTTAACAACAGcgta                                                <  1:1754081/148‑1 (MQ=255)
                                                                                                  gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             <  2:3097617/148‑1 (MQ=255)
                                                                                                  gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             <  2:3097593/148‑1 (MQ=255)
                                                                                                  gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             <  2:1454588/148‑1 (MQ=255)
                                                                                                  gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             <  2:1805031/148‑1 (MQ=255)
                                                                                                  gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             <  1:1032064/148‑1 (MQ=255)
                                                                                                   caatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgta                                            >  1:32857/1‑148 (MQ=255)
                                                                                                    aatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTAt                                           >  2:2794478/1‑148 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                      <  1:2648142/148‑1 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                      >  2:206291/1‑148 (MQ=255)
                                                                                                          atcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaaa                                     <  2:1649364/148‑1 (MQ=255)
                                                                                                           tcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACCGCGTACGTATGCTAAat                                    <  2:1698028/148‑1 (MQ=255)
                                                                                                           tcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAat                                    >  2:580743/1‑148 (MQ=255)
                                                                                                           tcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAat                                    <  2:1470203/148‑1 (MQ=255)
                                                                                                              tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATg                                 <  2:1244928/148‑1 (MQ=255)
                                                                                                              tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAAAAACAGCGTACGTATGCTAAATATg                                 >  1:197427/1‑148 (MQ=255)
                                                                                                               catGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATga                                >  2:2395955/1‑148 (MQ=255)
                                                                                                               catGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATga                                >  1:2073595/1‑148 (MQ=255)
                                                                                                                  gACCCGGATCACAGTCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaa                             <  1:3181177/148‑1 (MQ=255)
                                                                                                                    cccGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAt                           <  2:1754564/148‑1 (MQ=255)
                                                                                                                    cccGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAt                           <  1:2459984/148‑1 (MQ=255)
                                                                                                                      cGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtct                         <  1:1769640/148‑1 (MQ=255)
                                                                                                                       ggATCACAATCCATAATAATTTTTATATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                        <  2:433982/148‑1 (MQ=255)
                                                                                                                        gATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCTTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                       <  1:3109641/148‑1 (MQ=255)
                                                                                                                        gATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                       <  2:775980/148‑1 (MQ=255)
                                                                                                                        gATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                       <  2:727487/148‑1 (MQ=255)
                                                                                                                           cacaATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAg                    >  2:35816/1‑148 (MQ=255)
                                                                                                                             caATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTATACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                  >  1:1733103/1‑148 (MQ=255)
                                                                                                                                  cAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa             >  2:516944/1‑148 (MQ=255)
                                                                                                                                    gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAAc           <  1:1570539/148‑1 (MQ=255)
                                                                                                                                     aataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa          <  1:1031365/148‑1 (MQ=255)
                                                                                                                                     aataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa          <  1:1031361/148‑1 (MQ=255)
                                                                                                                                       taatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATTCTAAATATGAGAAATCTCATAGCGGATAAACATc        >  2:1968140/1‑148 (MQ=255)
                                                                                                                                       taatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAAAAACAGCGTAAGTATGCTAAATATGAGAAATCTCATAGCGGATAAACAta        >  1:1361626/1‑147 (MQ=255)
                                                                                                                                        aatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAAGATTAACAAAAGCGTACGTATTCTAAATATGAGAAATCTCATAGCGGATAAACATCg       >  1:1694534/1‑148 (MQ=255)
                                                                                                                                          tttttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTg     >  2:1020866/1‑148 (MQ=255)
                                                                                                                                            tttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaa   >  2:2745065/1‑148 (MQ=255)
                                                                                                                                            tttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaa   <  2:555911/148‑1 (MQ=255)
                                                                                                                                            tttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaa   <  2:825252/148‑1 (MQ=255)
                                                                                                                                             ttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaa  <  2:916712/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAA  >  CP009273/2248512‑2248800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑