BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,287,829	C→A	91.1%	S185S (TCG→TCT)	ccmF ←	heme lyase, CcmF subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,287,829	0	C	A	91.1%	536.9 / 47.2	203	S185S (TCG→TCT)	ccmF	heme lyase, CcmF subunit
Reads supporting (aligned to +/- strand): ref base C (9/9); new base A (82/103); total (91/112)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 205 total aligned reads displayed.

TTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGC  >  CP009273/2287685‑2287973

 tCGTAATAGGCCCATGCGGAACCGAGCACGATTCCGAGCGTCAGTAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCTCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAgaga                                                                                                                                              <  2:1364475/148‑1 (MQ=255)

 tCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAgaga                                                                                                                                              <  2:3011116/148‑1 (MQ=255)

 tCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAgaga                                                                                                                                              >  1:2377315/1‑148 (MQ=255)

 tCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAgaga                                                                                                                                              >  2:32592/1‑148 (MQ=255)

   ttAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCCGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGAAAAAGCAAACGCCACAGAGaaa                                                                                                                                            <  1:2917331/147‑1 (MQ=255)

    tAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAc                                                                                                                                           >  2:487312/1‑148 (MQ=255)

      aTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGATCAGCAAAGAAGAAATGGCAAAAGCAAACGCCACAGAGAAAccc                                                                                                                                         <  1:2149827/148‑1 (MQ=255)

       tAGGCCCATGCGGAACCGAGCAGGATGCCGAGCGTCAGTAAGATCCACGCTGCCAGCGTCCACGGACTATTAAAACGCGAATAAGTGCTGTCCAGACGCCCGCTCAGAAAAGAAGAAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                        <  1:1174779/148‑1 (MQ=255)

       tAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGTCACAGAGAAACCCa                                                                                                                                        >  1:733224/1‑148 (MQ=255)

       tAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGTCACAGAGAAACCCa                                                                                                                                        >  1:2203054/1‑148 (MQ=255)

       tAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                        >  1:829567/1‑148 (MQ=255)

         ggCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACg                                                                                                                                      >  2:602518/1‑148 (MQ=255)

         ggCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACg                                                                                                                                      <  2:1864206/148‑1 (MQ=255)

               tGCGGAACCGAGCACGTTGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGAATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCATTGGCAAAAGCAAACGCCACAGAGAAACCCACGTAcccc                                                                                                                                <  1:1461212/148‑1 (MQ=255)

                 cGGAACCGAGCACTATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGGTGTCCAGACGCCCGCTCAGCAAAGAAGCATTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCat                                                                                                                              <  1:3197641/148‑1 (MQ=255)

                    aaCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCAtata                                                                                                                           >  1:147715/1‑148 (MQ=255)

                     aCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaa                                                                                                                          <  2:3282123/148‑1 (MQ=255)

                      ccGAGCACTATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTAAGAAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaaa                                                                                                                         <  1:2626019/148‑1 (MQ=255)

                      ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATATGTGCTGTCCAGACGCCCGCTCAGAAAAGAAGAAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaaa                                                                                                                         <  1:2228467/148‑1 (MQ=255)

                       cGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAg                                                                                                                        <  2:2757616/148‑1 (MQ=255)

                       cGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAg                                                                                                                        >  2:897430/1‑148 (MQ=255)

                         aGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCa                                                                                                                      <  2:780693/148‑1 (MQ=255)

                          gCACGATGCGGTGCTTCTGGAAGATCCACGGTGCCTGCGTCCACGGACGATTAAAACGCGAATATGTGCTGTCCAGACGCCCGCTCAGAAAATAAGAATTGGAAAAAGAAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                     <  1:1540009/148‑1 (MQ=255)

                          gCACGATGCCGAGCTTCAGGAAGATCCACGCTGCCAGCGTCCACGGACTAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGAAAAGAAGAAATGGCAAACGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                     <  1:2788442/148‑1 (MQ=255)

                          gCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                     <  2:1196277/148‑1 (MQ=255)

                          gCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                     <  2:467284/148‑1 (MQ=255)

                             cGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAgg                                                                                                                  <  1:1934561/148‑1 (MQ=255)

                               aTGCCGAGCGTCAGTAAGATCCACGGTGCCTGCGTCCACGGACGATTAAAACGCGCATAAGTGCTTTCCAGACGCCCGATCAGAAAAGAAGAATTGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCg                                                                                                                <  1:1129205/148‑1 (MQ=255)

                                tGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCgg                                                                                                               <  1:1580309/148‑1 (MQ=255)

                                  ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                             <  2:613939/148‑1 (MQ=255)

                                  ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                             <  2:691560/148‑1 (MQ=255)

                                  ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                             <  2:691820/148‑1 (MQ=255)

                                    gAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATgg                                                                                                           >  2:2238549/1‑148 (MQ=255)

                                      gCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTAAGCAAAGAAGAAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGaa                                                                                                         <  1:2521657/148‑1 (MQ=255)

                                        gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGa                                                                                                       <  1:2472508/148‑1 (MQ=255)

                                         tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                      >  2:23618/1‑148 (MQ=255)

                                           aGGAAGATCCACGCTGCCAGCGTCCCCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                    <  1:2755305/148‑1 (MQ=255)

                                             gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                                  >  2:672936/1‑148 (MQ=255)

                                                  tCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCgg                                                                                             >  2:362596/1‑148 (MQ=255)

                                                     aCGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCACAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATc                                                                                          <  1:970899/148‑1 (MQ=255)

                                                          gCCCGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACACCATATAAAGAAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa                                                                                     >  2:1451201/1‑148 (MQ=255)

                                                          gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa                                                                                     >  1:2584/1‑148 (MQ=255)

                                                                  ccACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCggg                                                                             >  2:1855622/1‑148 (MQ=255)

                                                                    aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                           <  2:2796084/148‑1 (MQ=255)

                                                                    aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                           >  2:1500205/1‑148 (MQ=255)

                                                                     cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTa                                                                          <  1:1813041/148‑1 (MQ=255)

                                                                       gACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAg                                                                        <  1:878079/148‑1 (MQ=255)

                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                       <  1:2291401/148‑1 (MQ=255)

                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                       <  1:2183953/148‑1 (MQ=255)

                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                       >  2:756668/1‑148 (MQ=255)

                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                       <  1:1363153/148‑1 (MQ=255)

                                                                           aGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcg                                                                    >  1:19981/1‑148 (MQ=255)

                                                                             tAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTGAAGATcgcg                                                                  >  2:2604717/1‑148 (MQ=255)

                                                                                 aCGCGCATATGTGCTGTCCAGACGCCCGCTCCGCAAACAAGCAAGGGCAAAAGAAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCt                                                              <  1:1766763/148‑1 (MQ=255)

                                                                                 aCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCt                                                              <  1:92065/148‑1 (MQ=255)

                                                                                 aCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCt                                                              <  1:1058640/148‑1 (MQ=255)

                                                                                  cgcgCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCtt                                                             >  2:2114036/1‑148 (MQ=255)

                                                                                     gcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCaa                                                          <  2:2202413/148‑1 (MQ=255)

                                                                                     gcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCaa                                                          <  2:1853873/148‑1 (MQ=255)

                                                                                      cATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                         <  2:20498/148‑1 (MQ=255)

                                                                                          aGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCggg                                                     <  2:1088757/148‑1 (MQ=255)

                                                                                          aGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCggg                                                     >  2:802705/1‑148 (MQ=255)

                                                                                           gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                    >  1:1519699/1‑148 (MQ=255)

                                                                                           gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                    >  1:3160818/1‑148 (MQ=255)

                                                                                            tGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGaa                                                   >  2:1080165/1‑148 (MQ=255)

                                                                                             gCTGTCCAGACGCCCGCTCAGCAATGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAg                                                  <  1:2571261/148‑1 (MQ=255)

                                                                                             gCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAg                                                  <  1:791825/148‑1 (MQ=255)

                                                                                             gCTGACCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAg                                                  <  1:914544/148‑1 (MQ=255)

                                                                                              cTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCAGATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGt                                                 >  2:2113088/1‑148 (MQ=255)

                                                                                                gTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTc                                               >  1:2700471/1‑148 (MQ=255)

                                                                                                 tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCg                                              >  2:398869/1‑148 (MQ=255)

                                                                                                      aCGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAAc                                         >  1:2088679/1‑148 (MQ=255)

                                                                                                      aCGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAAc                                         >  1:2181494/1‑148 (MQ=255)

                                                                                                       cGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACg                                        >  2:1717051/1‑148 (MQ=255)

                                                                                                           cGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                    >  1:3248249/1‑148 (MQ=255)

                                                                                                                aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagaga                               <  1:362596/148‑1 (MQ=255)

                                                                                                                 gCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGaa                              >  2:2702644/1‑148 (MQ=255)

                                                                                                                     agaagCAATGGCAAAAGCAAACGCCACAGAGAAAGCCCCGTACCCCATATACAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACggg                          <  2:79710/148‑1 (MQ=255)

                                                                                                                     agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACggg                          >  1:2776733/1‑148 (MQ=255)

                                                                                                                     agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACggg                          <  2:828659/148‑1 (MQ=255)

                                                                                                                     agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACggg                          <  2:2089736/148‑1 (MQ=255)

                                                                                                                     agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACggg                          <  2:1754114/148‑1 (MQ=255)

                                                                                                                      gaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGt                         <  1:23618/148‑1 (MQ=255)

                                                                                                                          cAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGACCGGGTTaga                     >  2:3082236/1‑148 (MQ=255)

                                                                                                                          cAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTaga                     >  1:350614/1‑148 (MQ=255)

                                                                                                                          cAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTaga                     >  2:1537564/1‑148 (MQ=255)

                                                                                                                               gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa                >  1:1942693/1‑148 (MQ=255)

                                                                                                                               gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa                <  2:2999656/148‑1 (MQ=255)

                                                                                                                                 aaaaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAag              <  2:2515719/148‑1 (MQ=255)

                                                                                                                                   aaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAagag            >  1:1655626/1‑148 (MQ=255)

                                                                                                                                    aGCAAACGTCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAgaga           <  2:733224/148‑1 (MQ=255)

                                                                                                                                     gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGTCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa          >  1:2139429/1‑148 (MQ=255)

                                                                                                                                     gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa          <  1:2616684/148‑1 (MQ=255)

                                                                                                                                       aaaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATg        <  1:2714807/148‑1 (MQ=255)

                                                                                                                                       aaaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATg        <  2:560582/148‑1 (MQ=255)

                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAGGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa       <  1:2116501/148‑1 (MQ=255)

                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGACTGa       >  1:2426636/1‑148 (MQ=255)

                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa       <  1:3093894/148‑1 (MQ=255)

                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa       <  1:602518/148‑1 (MQ=255)

                                                                                                                                           gCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACa    <  2:2671762/148‑1 (MQ=255)

TTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGC  >  CP009273/2287685‑2287973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`