Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,342,897 C→A 100% D19Y (GAC→TAC)  inaA ← acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,342,8970CA98.4% 587.3 / 0.9 187D19Y (GAC→TAC) inaAacid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (82/101);  total (83/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

AATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTT  >  CP009273/2342754‑2343041
                                                                                                                                               |                                                                                                                                                
taTCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGccc                                                                                                                                              <  1:2202471/147‑1 (MQ=255)
    gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTg                                                                                                                                          <  2:2737524/148‑1 (MQ=255)
     ttGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGt                                                                                                                                         >  1:1405666/1‑148 (MQ=255)
            gCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccac                                                                                                                                  >  1:3010129/1‑148 (MQ=255)
             ccGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccacc                                                                                                                                 <  1:390644/148‑1 (MQ=255)
             ccGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccacc                                                                                                                                 <  1:1803895/148‑1 (MQ=255)
             ccGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccacc                                                                                                                                 <  1:132438/148‑1 (MQ=255)
                aaCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGt                                                                                                                              <  2:138336/148‑1 (MQ=255)
                aaCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGt                                                                                                                              >  2:1917517/1‑148 (MQ=255)
                aaCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGt                                                                                                                              <  1:1596174/148‑1 (MQ=255)
                 aCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTg                                                                                                                             <  1:2732071/148‑1 (MQ=255)
                      tAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaa                                                                                                                        >  1:2913086/1‑148 (MQ=255)
                      tAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaa                                                                                                                        >  1:3179325/1‑148 (MQ=255)
                       aaCGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaaa                                                                                                                       <  1:1380498/148‑1 (MQ=255)
                        aCGTACGGAATGAAACAGATGATGAGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAAt                                                                                                                      >  1:1544540/1‑148 (MQ=255)
                         cGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAAtt                                                                                                                     >  1:2438932/1‑148 (MQ=255)
                         cGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAAtt                                                                                                                     >  1:2441618/1‑148 (MQ=255)
                         cGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAAtt                                                                                                                     >  1:285083/1‑148 (MQ=255)
                           tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcg                                                                                                                   <  1:749910/148‑1 (MQ=255)
                                aaTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                              <  2:3292274/148‑1 (MQ=255)
                                 aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTAc                                                                                                             >  2:790129/1‑148 (MQ=255)
                                 aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTAc                                                                                                             >  1:701361/1‑148 (MQ=255)
                                  tGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACt                                                                                                            >  1:555697/1‑148 (MQ=255)
                                  tGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACt                                                                                                            <  1:719313/148‑1 (MQ=255)
                                   gAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACtt                                                                                                           <  2:592270/148‑1 (MQ=255)
                                    aaaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACttt                                                                                                          <  1:2639221/148‑1 (MQ=255)
                                        aGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTg                                                                                                      <  2:2607035/148‑1 (MQ=255)
                                          atgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaa                                                                                                    <  1:1019511/148‑1 (MQ=255)
                                          atgatgCGTCATGCGATTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCTACCCAGTTGCCCTCTGTTGCCCACCAGTGATTATATTCGTGGTACTTTGCTGaa                                                                                                    <  2:1191868/148‑1 (MQ=255)
                                            gatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGGGGTGGAGTGATTAAATTCGTCGTACTTTGCTGAAAc                                                                                                  <  2:1864821/148‑1 (MQ=255)
                                            gatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAAc                                                                                                  >  1:938979/1‑148 (MQ=255)
                                             atgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACt                                                                                                 >  1:748219/1‑148 (MQ=255)
                                               gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCCTTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGAGTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               >  2:1415912/1‑148 (MQ=255)
                                               gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               >  2:2466977/1‑148 (MQ=255)
                                               gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               <  2:1349944/148‑1 (MQ=255)
                                               gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               >  2:2027832/1‑148 (MQ=255)
                                               gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               >  1:2032414/1‑148 (MQ=255)
                                                  tCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCAt                                                                                            >  1:1955256/1‑148 (MQ=255)
                                                   cATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATa                                                                                           <  1:141525/148‑1 (MQ=255)
                                                   cATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATa                                                                                           >  2:904592/1‑148 (MQ=255)
                                                    aTGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAc                                                                                          >  2:1859260/1‑148 (MQ=255)
                                                      gcgcTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATAccc                                                                                        <  1:326979/148‑1 (MQ=255)
                                                        gcTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTa                                                                                      <  2:2850439/148‑1 (MQ=255)
                                                                tacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGt                                                                              >  1:247392/1‑148 (MQ=255)
                                                                 acaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTc                                                                             >  1:2633297/1‑148 (MQ=255)
                                                                    gCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAtt                                                                          <  1:692405/148‑1 (MQ=255)
                                                                    gCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACAATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTATTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTCTCAta                                                                          >  1:520026/1‑147 (MQ=255)
                                                                     cTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAttt                                                                         >  1:2201699/1‑148 (MQ=255)
                                                                        tttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTtta                                                                      <  2:1788818/148‑1 (MQ=255)
                                                                         ttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttat                                                                     <  1:1265398/148‑1 (MQ=255)
                                                                         ttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttat                                                                     <  2:378316/148‑1 (MQ=255)
                                                                           gCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTTGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttatta                                                                   <  1:2684816/148‑1 (MQ=255)
                                                                           gCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttatta                                                                   <  2:478177/148‑1 (MQ=255)
                                                                           gCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttatta                                                                   <  1:1137843/148‑1 (MQ=255)
                                                                                tGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGAc                                                              >  2:3157251/1‑148 (MQ=255)
                                                                                tGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGAc                                                              >  2:921514/1‑148 (MQ=255)
                                                                                 gcgcTCGACGCATTGCACCCCGCTCATACAATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTTATTAAATTGGTCGTACTTTGCTTAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACa                                                             <  1:1047520/148‑1 (MQ=255)
                                                                                  cgcTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACaa                                                            <  1:1249318/148‑1 (MQ=255)
                                                                                     tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         <  1:1270968/148‑1 (MQ=255)
                                                                                     tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTTAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         <  1:1140015/148‑1 (MQ=255)
                                                                                     tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         <  1:2408848/148‑1 (MQ=255)
                                                                                     tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         <  1:2791359/148‑1 (MQ=255)
                                                                                     tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         <  1:3261804/148‑1 (MQ=255)
                                                                                        aCGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACta                                                      >  1:608317/1‑148 (MQ=255)
                                                                                          gCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACtata                                                    <  2:1835797/148‑1 (MQ=255)
                                                                                           cATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAg                                                   <  2:1941745/148‑1 (MQ=255)
                                                                                              tGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtg                                                >  2:649430/1‑148 (MQ=255)
                                                                                              tGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtg                                                >  1:3090039/1‑148 (MQ=255)
                                                                                               gCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtgg                                               <  2:421579/148‑1 (MQ=255)
                                                                                                   cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAc                                           >  1:815764/1‑148 (MQ=255)
                                                                                                     cGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCt                                         >  2:2957118/1‑148 (MQ=255)
                                                                                                      gCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTg                                        <  2:1136502/148‑1 (MQ=255)
                                                                                                      gCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTg                                        <  1:3092626/148‑1 (MQ=255)
                                                                                                        tCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAg                                      >  2:209042/1‑148 (MQ=255)
                                                                                                        tCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAg                                      >  2:2151950/1‑148 (MQ=255)
                                                                                                        tCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAg                                      >  2:484853/1‑148 (MQ=255)
                                                                                                         cATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAgg                                     >  1:1663816/1‑148 (MQ=255)
                                                                                                         cATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAgg                                     >  1:2516331/1‑148 (MQ=255)
                                                                                                           tACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaa                                   >  1:466800/1‑148 (MQ=255)
                                                                                                             ccATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaaaa                                 >  2:120010/1‑148 (MQ=255)
                                                                                                             ccATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaaaa                                 >  2:3216092/1‑148 (MQ=255)
                                                                                                              cATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAAt                                <  1:2709625/148‑1 (MQ=255)
                                                                                                              cATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAAt                                <  1:2709579/148‑1 (MQ=255)
                                                                                                                  aCGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTa                            <  2:1797015/148‑1 (MQ=255)
                                                                                                                  aCGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTa                            <  1:669968/148‑1 (MQ=255)
                                                                                                                        aTAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGaaa                      <  2:290218/148‑1 (MQ=255)
                                                                                                                         tAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTTACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGactc                     >  2:2480234/1‑145 (MQ=255)
                                                                                                                            ttAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTCTAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtg                  >  2:501004/1‑148 (MQ=255)
                                                                                                                             tAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtgt                 <  2:349366/148‑1 (MQ=255)
                                                                                                                             tAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtgt                 <  2:2438932/148‑1 (MQ=255)
                                                                                                                             tAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtgt                 <  2:1405666/148‑1 (MQ=255)
                                                                                                                             tAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACgtgt                 <  2:1021612/148‑1 (MQ=255)
                                                                                                                               ggCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCg               >  2:3009178/1‑148 (MQ=255)
                                                                                                                                gCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt              <  2:3006049/148‑1 (MQ=255)
                                                                                                                                gCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt              >  1:3196206/1‑148 (MQ=255)
                                                                                                                                 ctctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTa             <  2:240233/148‑1 (MQ=255)
                                                                                                                                      cAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAAt        <  1:2466977/148‑1 (MQ=255)
                                                                                                                                      cAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAAt        <  1:2027832/148‑1 (MQ=255)
                                                                                                                                      cAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCAGACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAAt        <  1:359690/148‑1 (MQ=255)
                                                                                                                                         cccAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGaa     >  1:745813/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTT  >  CP009273/2342754‑2343041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑