Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,402,924 A→T 14.5% Q195L (CAG→CTG)  yfbR → 5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,402,9240AT14.5% 538.2 / 74.8 200Q195L (CAG→CTG) yfbR5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (88/82);  new base T (15/14);  total (104/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 202 total aligned reads displayed.

TGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCAAC  >  CP009273/2402777‑2403071
                                                                                                                                                   |                                                                                                                                                   
tGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCa                                                                                                                                                     <  2:1279337/148‑1 (MQ=255)
 ggAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAg                                                                                                                                                    >  1:1705949/1‑148 (MQ=255)
  gaagaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAgg                                                                                                                                                   >  1:3197399/1‑148 (MQ=255)
  gaagaaATCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATAAGCCAgg                                                                                                                                                   >  1:2918258/1‑148 (MQ=255)
   aagaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGa                                                                                                                                                  <  1:1839580/148‑1 (MQ=255)
    agaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGAt                                                                                                                                                 >  1:1866333/1‑148 (MQ=255)
      taCTCGGGTCCGGAATTAATGAATTCTTTCTGGCAAAAACGCGTCTGGATGCGAGGCTTGAAGAGCTTCGAATCCAGGTGATGGACTTCTTCATGGAAATATTTGTTCTCATCTTCAATCTTTCTCTCTATTATATTAGCCATGATTc                                                                                                                                               <  2:439520/147‑1 (MQ=255)
      aaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTc                                                                                                                                               >  1:956241/1‑148 (MQ=255)
      aaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTc                                                                                                                                               >  2:1994933/1‑148 (MQ=255)
      aaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTc                                                                                                                                               <  2:439821/148‑1 (MQ=255)
      aaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTc                                                                                                                                               <  2:439838/148‑1 (MQ=255)
      aaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTc                                                                                                                                               >  2:692591/1‑148 (MQ=255)
        cTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCAc                                                                                                                                             >  1:582459/1‑148 (MQ=255)
         tCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGAGGAGATTAGCCAGGATTCAcc                                                                                                                                            >  2:2799950/1‑148 (MQ=255)
          cgcgGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCg                                                                                                                                           >  1:1759861/1‑148 (MQ=255)
          cgcgGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCg                                                                                                                                           <  1:86499/148‑1 (MQ=255)
               ccGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTa                                                                                                                                      >  2:485881/1‑148 (MQ=255)
                 ggAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAag                                                                                                                                    <  2:191146/148‑1 (MQ=255)
                   aaaTAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAagca                                                                                                                                  >  1:487528/1‑148 (MQ=255)
                     ataatGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAagcagc                                                                                                                                <  2:1712366/148‑1 (MQ=255)
                     ataatGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAagcagc                                                                                                                                >  2:88474/1‑148 (MQ=255)
                      taatGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGcc                                                                                                                               >  2:382080/1‑148 (MQ=255)
                       aatGAATTCTTGCTGGCAAAAACGAGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCg                                                                                                                              >  2:2290659/1‑148 (MQ=255)
                          gAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAg                                                                                                                           >  2:2156029/1‑148 (MQ=255)
                           aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGt                                                                                                                          <  2:2822183/148‑1 (MQ=255)
                           aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGt                                                                                                                          <  2:1082015/148‑1 (MQ=255)
                           aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGt                                                                                                                          >  1:39696/1‑148 (MQ=255)
                            aTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTc                                                                                                                         >  2:2558122/1‑148 (MQ=255)
                                 tGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGt                                                                                                                    <  1:783636/148‑1 (MQ=255)
                                      gCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCAt                                                                                                               >  1:1151457/1‑148 (MQ=255)
                                      gCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCAt                                                                                                               <  2:407346/148‑1 (MQ=255)
                                        aaaaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCa                                                                                                             <  1:2739610/148‑1 (MQ=255)
                                          aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:2689596/1‑148 (MQ=255)
                                          aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:810163/1‑148 (MQ=255)
                                          aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:2804711/1‑148 (MQ=255)
                                          aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:3240667/1‑148 (MQ=255)
                                            aCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGAGGAGATTAGCCCGGAGTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGcc                                                                                                         >  2:1718304/1‑147 (MQ=255)
                                             cgcgACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCaa                                                                                                        <  2:1810071/148‑1 (MQ=255)
                                               cgACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGAGTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCACTa                                                                                                      >  2:2033032/1‑148 (MQ=255)
                                               cgACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATa                                                                                                      <  2:1453619/148‑1 (MQ=255)
                                                gACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATaa                                                                                                     <  2:1751463/148‑1 (MQ=255)
                                                  cTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAgc                                                                                                   >  1:2899004/1‑148 (MQ=255)
                                                  cTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAgc                                                                                                   >  1:437928/1‑148 (MQ=255)
                                                    ggAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAgcgc                                                                                                 <  2:914942/148‑1 (MQ=255)
                                                     gAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGcc                                                                                                >  1:2386380/1‑148 (MQ=255)
                                                         cGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGAt                                                                                            >  1:1336984/1‑148 (MQ=255)
                                                                tGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     >  1:344388/1‑148 (MQ=255)
                                                                tGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     >  1:215475/1‑148 (MQ=255)
                                                                tGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     >  1:215335/1‑148 (MQ=255)
                                                                tGAAGCGCGTCGCAGCCAGGAGAGGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     <  1:1957710/148‑1 (MQ=255)
                                                                  aaGCGCGTCGCAGACAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGTCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCa                                                                                   <  2:520333/148‑1 (MQ=255)
                                                                    gcgcGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAgg                                                                                 <  2:1627575/148‑1 (MQ=255)
                                                                      gcgTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGct                                                                               <  1:654786/148‑1 (MQ=255)
                                                                       cgTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGctc                                                                              >  2:1037933/1‑148 (MQ=255)
                                                                       cgTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGctc                                                                              >  2:1037864/1‑148 (MQ=255)
                                                                               ccAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCaaa                                                                      >  2:3268025/1‑148 (MQ=255)
                                                                               ccAGGAGATGGACTACTTCATGGAAATATTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCaaa                                                                      <  1:3045678/148‑1 (MQ=255)
                                                                                   gagaTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACgg                                                                  <  1:981094/148‑1 (MQ=255)
                                                                                   gagaTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACgg                                                                  >  1:528814/1‑148 (MQ=255)
                                                                                    agaTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGa                                                                 >  2:233295/1‑148 (MQ=255)
                                                                                      aTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGaaa                                                               >  1:707078/1‑148 (MQ=255)
                                                                                           ctactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCa                                                          <  2:3091917/148‑1 (MQ=255)
                                                                                           ctactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTATGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCa                                                          <  1:1344232/148‑1 (MQ=255)
                                                                                             actTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATc                                                        >  2:1149460/1‑148 (MQ=255)
                                                                                                tCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCggg                                                     <  2:1337061/148‑1 (MQ=255)
                                                                                                tCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCggg                                                     >  1:2277700/1‑148 (MQ=255)
                                                                                                    ggAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatca                                                 <  2:2918258/148‑1 (MQ=255)
                                                                                                     gAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcat                                                <  2:2476446/148‑1 (MQ=255)
                                                                                                      aaaTATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcatc                                               >  1:2356652/1‑148 (MQ=255)
                                                                                                      aaaTATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcatc                                               <  2:3230741/148‑1 (MQ=255)
                                                                                                      aaaTATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcatc                                               <  2:3230728/148‑1 (MQ=255)
                                                                                                        atatTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATcac                                             >  1:1939811/1‑148 (MQ=255)
                                                                                                          atTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATcacca                                           >  2:3215582/1‑148 (MQ=255)
                                                                                                          atTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATcacca                                           <  2:844103/148‑1 (MQ=255)
                                                                                                               ttCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACaa                                      >  1:3184023/1‑148 (MQ=255)
                                                                                                               ttCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACaa                                      <  2:1847124/148‑1 (MQ=255)
                                                                                                                   cAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCg                                  >  1:1593169/1‑148 (MQ=255)
                                                                                                                      cTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCa                               <  1:2978621/148‑1 (MQ=255)
                                                                                                                       ttCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCAt                              >  2:1314936/1‑148 (MQ=255)
                                                                                                                       ttCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCAt                              >  2:2954123/1‑148 (MQ=255)
                                                                                                                            tCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGa                         <  1:1037933/148‑1 (MQ=255)
                                                                                                                            tCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGa                         <  1:1037864/148‑1 (MQ=255)
                                                                                                                             cTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGAt                        >  1:414704/1‑148 (MQ=255)
                                                                                                                             cTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGAt                        >  2:770891/1‑148 (MQ=255)
                                                                                                                             cTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGAt                        >  1:1898974/1‑148 (MQ=255)
                                                                                                                              tttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATa                       >  2:1673064/1‑148 (MQ=255)
                                                                                                                              tttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATa                       <  1:1409327/148‑1 (MQ=255)
                                                                                                                               ttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      <  2:318047/148‑1 (MQ=255)
                                                                                                                               ttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      <  1:1098990/148‑1 (MQ=255)
                                                                                                                               ttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      >  1:2067791/1‑148 (MQ=255)
                                                                                                                                  gCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATgg                   <  2:1884588/148‑1 (MQ=255)
                                                                                                                                  gCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATgg                   <  2:215335/148‑1 (MQ=255)
                                                                                                                                  gCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATgg                   <  2:215475/148‑1 (MQ=255)
                                                                                                                                   cTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGt                  <  1:485848/148‑1 (MQ=255)
                                                                                                                                     cGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGa                <  1:2569845/148‑1 (MQ=255)
                                                                                                                                      gATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGaa               <  1:2156852/148‑1 (MQ=255)
                                                                                                                                      gATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGaa               <  1:3259185/148‑1 (MQ=255)
                                                                                                                                       aTGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAAc              >  1:2640946/1‑148 (MQ=255)
                                                                                                                                          agaTTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGt           >  1:2705755/1‑148 (MQ=255)
                                                                                                                                                  cAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCaa   >  1:2587347/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCAAC  >  CP009273/2402777‑2403071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑