Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,456,705 C→A 92.1% S72Y (TCC→TAC)  yfdF → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,456,7050CA92.1% 559.9 / 38.8 202S72Y (TCC→TAC) yfdFuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (7/9);  new base A (80/106);  total (87/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 202 total aligned reads displayed.

GATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGA  >  CP009273/2456560‑2456848
                                                                                                                                                 |                                                                                                                                               
 atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTc                                                                                                                                              <  2:1202898/148‑1 (MQ=255)
   gaAGTTAATTGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAg                                                                                                                                            <  2:2862652/148‑1 (MQ=255)
    aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGt                                                                                                                                           <  2:356971/148‑1 (MQ=255)
      gTTAATGTATTAGTACAAGATTAAAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTa                                                                                                                                         <  1:3140047/148‑1 (MQ=255)
       ttAATGGATTAGTACAAGAGTTCAAAAACCTTTTTATTGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTAc                                                                                                                                        <  2:2295013/148‑1 (MQ=255)
       ttAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTAc                                                                                                                                        <  2:1828970/148‑1 (MQ=255)
        tAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACt                                                                                                                                       >  2:3078939/1‑148 (MQ=255)
         aaTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACtt                                                                                                                                      >  1:2889573/1‑148 (MQ=255)
          aTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTta                                                                                                                                     >  2:3165850/1‑148 (MQ=255)
             gATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatat                                                                                                                                  <  2:624040/148‑1 (MQ=255)
              aTTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatatt                                                                                                                                 >  1:2795524/1‑148 (MQ=255)
              aTTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatatt                                                                                                                                 >  2:1082316/1‑148 (MQ=255)
               ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattg                                                                                                                                <  2:3057389/148‑1 (MQ=255)
               ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTAGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattg                                                                                                                                <  2:3223510/148‑1 (MQ=255)
                tAGTACAAGATTTAAAAAACCTTTTTAATGGTAAGGAAGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattga                                                                                                                               <  1:655829/148‑1 (MQ=255)
                  gTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                             <  2:1736043/148‑1 (MQ=255)
                  gTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                             <  1:1090999/148‑1 (MQ=255)
                   tACAAGAGTTCAAAAACCTTTTTAATGTTAATGAAGTAATAAGCACCTGTATTAAACATCTACTTGATCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatat                                                                                                                            <  1:2088108/148‑1 (MQ=255)
                    aCAAGAGTTCAAAAACATTTTTAATGGTAAGGAAGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatatt                                                                                                                           <  1:922228/148‑1 (MQ=255)
                           ttCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCa                                                                                                                    >  2:696875/1‑148 (MQ=255)
                           ttCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCa                                                                                                                    >  2:952531/1‑148 (MQ=255)
                            tCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCaa                                                                                                                   >  2:1345070/1‑148 (MQ=255)
                            tCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCaa                                                                                                                   >  2:1344602/1‑148 (MQ=255)
                              aaaaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATg                                                                                                                 <  2:279719/148‑1 (MQ=255)
                                aaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGAt                                                                                                               >  2:2544261/1‑148 (MQ=255)
                                 aaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATa                                                                                                              >  1:184589/1‑148 (MQ=255)
                                     tttttAATGGTAAGTAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGa                                                                                                          <  1:657112/148‑1 (MQ=255)
                                       tttAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAcc                                                                                                        >  1:2515066/1‑148 (MQ=255)
                                         tAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCat                                                                                                      <  2:1657533/148‑1 (MQ=255)
                                            ttgTAAGGAAGGAATAAGCACCTGTATTATACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAtt                                                                                                   <  2:2139847/146‑1 (MQ=255)
                                            tGGTAAGTAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAtt                                                                                                   <  2:476328/148‑1 (MQ=255)
                                            tGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAtt                                                                                                   <  2:2678172/148‑1 (MQ=255)
                                              gTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTAc                                                                                                 >  2:1527065/1‑148 (MQ=255)
                                                aaggaaggAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTa                                                                                               <  1:2745694/148‑1 (MQ=255)
                                                    aaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTgg                                                                                           >  2:377255/1‑148 (MQ=255)
                                                    aaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTgg                                                                                           <  2:761058/148‑1 (MQ=255)
                                                      ggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTa                                                                                         <  2:1281914/148‑1 (MQ=255)
                                                      ggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTa                                                                                         >  1:576575/1‑148 (MQ=255)
                                                        aaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATc                                                                                       >  2:814496/1‑148 (MQ=255)
                                                         aTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCg                                                                                      <  2:1312376/148‑1 (MQ=255)
                                                         aTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCg                                                                                      <  1:2978878/148‑1 (MQ=255)
                                                            aGCACCTGTATTAAACATCTAATTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACa                                                                                   <  2:1020761/148‑1 (MQ=255)
                                                             gCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACaa                                                                                  >  1:2658216/1‑148 (MQ=255)
                                                                ccTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCa                                                                               <  1:418807/148‑1 (MQ=255)
                                                                ccTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCa                                                                               <  2:161476/148‑1 (MQ=255)
                                                                 cTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGTTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGa                                                                                                          >  1:1283462/1‑120 (MQ=255)
                                                                   gTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGa                                                                            <  1:375713/148‑1 (MQ=255)
                                                                   gTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGa                                                                            <  1:3019240/148‑1 (MQ=255)
                                                                      ttAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAcc                                                                         >  2:2788290/1‑148 (MQ=255)
                                                                      ttAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAcc                                                                         >  2:2458536/1‑148 (MQ=255)
                                                                        aaaCATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCaa                                                                       <  1:2584835/148‑1 (MQ=255)
                                                                        aaaCATATACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCaa                                                                       <  1:2450390/148‑1 (MQ=255)
                                                                             tatACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                  <  2:3063934/146‑1 (MQ=255)
                                                                             tCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                  <  1:805241/148‑1 (MQ=255)
                                                                             tCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                  >  1:949570/1‑148 (MQ=255)
                                                                               tACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAAtt                                                                <  2:908639/148‑1 (MQ=255)
                                                                                aCTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTa                                                               >  1:1674642/1‑148 (MQ=255)
                                                                                   tGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCt                                                            >  1:2829235/1‑148 (MQ=255)
                                                                                      gCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCg                                                         >  1:377046/1‑148 (MQ=255)
                                                                                       cTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGa                                                        >  2:1572941/1‑148 (MQ=255)
                                                                                       cTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGa                                                        >  2:2085057/1‑148 (MQ=255)
                                                                                          ataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACt                                                     <  1:2378453/148‑1 (MQ=255)
                                                                                           taAAAAACGCCATACGAGTAAACGACGATACTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACta                                                    <  2:2127933/148‑1 (MQ=255)
                                                                                            aaaaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACtat                                                   <  2:2909764/148‑1 (MQ=255)
                                                                                             aaaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACtata                                                  >  1:1691681/1‑148 (MQ=255)
                                                                                               aaaCGCCCACCGAGTACACGCCGATCCTTATAGATTTAATATCAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACGATaaa                                                <  1:1529568/148‑1 (MQ=255)
                                                                                               aaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATaaa                                                <  1:422940/148‑1 (MQ=255)
                                                                                                 aCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGa                                              <  1:1345070/148‑1 (MQ=255)
                                                                                                 aCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGa                                              <  1:1344602/148‑1 (MQ=255)
                                                                                                 aCGACATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGa                                              <  2:2649231/148‑1 (MQ=255)
                                                                                                  cGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGAc                                             >  1:2868611/1‑148 (MQ=255)
                                                                                                    ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACaa                                           >  1:1401252/1‑148 (MQ=255)
                                                                                                      aTACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                         >  1:3286775/1‑148 (MQ=255)
                                                                                                      aTACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                         <  2:1087857/148‑1 (MQ=255)
                                                                                                       tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGa                                        <  2:2180585/148‑1 (MQ=255)
                                                                                                        aCGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGaa                                       <  1:2835504/148‑1 (MQ=255)
                                                                                                              aaaCGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTc                                 <  1:1026048/148‑1 (MQ=255)
                                                                                                              aaaCGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTc                                 <  1:1024096/148‑1 (MQ=255)
                                                                                                               aaCGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTcc                                <  2:1459719/148‑1 (MQ=255)
                                                                                                                      aTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAt                         >  1:1520668/1‑148 (MQ=255)
                                                                                                                           tataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTa                    >  1:1072745/1‑148 (MQ=255)
                                                                                                                           tataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTa                    >  1:1731380/1‑148 (MQ=255)
                                                                                                                             taGATTTAATATTAATAATTACTGAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTTGTATCGACAACCTAGATCAAATAGAATTACCTGCGATCTATAAAGACAAAGAACTCGTCCGTACTATCATTAAt                  <  2:433379/148‑1 (MQ=255)
                                                                                                                             taGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAAt                  <  2:2215930/148‑1 (MQ=255)
                                                                                                                              aGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCAAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                 <  2:1777390/148‑1 (MQ=255)
                                                                                                                              aGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                 >  2:2812978/1‑148 (MQ=255)
                                                                                                                                 tttAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGaca              <  2:2598176/148‑1 (MQ=255)
                                                                                                                                  ttaatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaa             >  2:1430630/1‑148 (MQ=255)
                                                                                                                                    aatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaaca           <  2:184589/148‑1 (MQ=255)
                                                                                                                                     atattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt          <  2:3289164/148‑1 (MQ=255)
                                                                                                                                      tattaataATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATa         >  2:1623337/1‑148 (MQ=255)
                                                                                                                                         taataaTTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGtt      >  1:716718/1‑148 (MQ=255)
                                                                                                                                          aataatTACTCATTTAATTATATTGATATTTACTCCAATTATACATACCATATTACTATTGGTATCGACAACCAAGAAACAATAGAATTAACTGCTAAATATAAAGACAAAGAACTCGTCCGTACTATCATTATTGACAACATAGTTg     <  2:2845545/148‑1 (MQ=255)
                                                                                                                                          aataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTg     <  2:2658216/148‑1 (MQ=255)
                                                                                                                                           ataatTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga    >  2:3111708/1‑148 (MQ=255)
                                                                                                                                            taatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGTCAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgag   <  2:330710/148‑1 (MQ=255)
                                                                                                                                            taatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgag   >  1:1993462/1‑148 (MQ=255)
                                                                                                                                            taatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgag   >  1:1995327/1‑148 (MQ=255)
                                                                                                                                             aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgaga  >  2:1202698/1‑148 (MQ=255)
                                                                                                                                             aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgaga  >  1:544583/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGA  >  CP009273/2456560‑2456848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑