BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,524,056	C→A	84.8%	A179A (GCG→GCT)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,524,056	0	C	A	84.8%	358.0 / 73.1	165	A179A (GCG→GCT)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base C (18/7); new base A (75/65); total (93/72)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 165 total aligned reads displayed.

ATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523910‑2524200

   ttCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACAGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTa                                                                                                                                              <  1:1460555/148‑1 (MQ=255)

    tCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAc                                                                                                                                             >  2:1264296/1‑148 (MQ=255)

     cATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAca                                                                                                                                            <  2:44438/148‑1 (MQ=255)

      atatCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcag                                                                                                                                           <  1:2788257/148‑1 (MQ=255)

        atCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggc                                                                                                                                         >  2:3177199/1‑148 (MQ=255)

        atCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggc                                                                                                                                         >  1:2057483/1‑148 (MQ=255)

        atCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggc                                                                                                                                         >  2:1537589/1‑148 (MQ=255)

          cGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctc                                                                                                                                       >  1:1775837/1‑148 (MQ=255)

          cGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctc                                                                                                                                       >  1:101964/1‑148 (MQ=255)

          cGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctc                                                                                                                                       <  2:2302885/148‑1 (MQ=255)

            ccAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcag                                                                                                                                     >  2:1645578/1‑148 (MQ=255)

            ccAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcag                                                                                                                                     >  2:1645648/1‑148 (MQ=255)

              aaaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcaggc                                                                                                                                   <  1:1403675/148‑1 (MQ=255)

                  aTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCtgtg                                                                                                                               >  1:2941256/1‑148 (MQ=255)

                  aTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCtgtg                                                                                                                               >  1:2941291/1‑148 (MQ=255)

                  aTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtgtg                                                                                                                               <  1:1510436/148‑1 (MQ=255)

                     aaGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtggtg                                                                                                                            >  1:2983975/1‑148 (MQ=255)

                      aGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGc                                                                                                                           <  1:3080391/148‑1 (MQ=255)

                       gCCCTCTTGTTGAATGCTGTTAAGAAGAAGTTCAAAGTTTAGCTCTCTACCGTGATGCGCCGCTACTTTCATGATAATCACCGCTTCTTTGAGATTCGGTTTATCAATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGcc                                                                                                                          >  1:3180957/1‑148 (MQ=255)

                       gCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGcc                                                                                                                          >  1:621590/1‑148 (MQ=255)

                          cGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCt                                                                                                                       >  2:3173894/1‑148 (MQ=255)

                              tgttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACgg                                                                                                                   >  1:307003/1‑148 (MQ=255)

                              tgttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACgg                                                                                                                   >  1:846038/1‑148 (MQ=255)

                               gttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACggg                                                                                                                  <  1:2467922/148‑1 (MQ=255)

                               gttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACggg                                                                                                                  <  1:122130/148‑1 (MQ=255)

                                 tgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGtt                                                                                                                <  1:2244450/148‑1 (MQ=255)

                                    aTGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTg                                                                                                             <  1:732016/148‑1 (MQ=255)

                                    aTGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTg                                                                                                             <  1:2391421/148‑1 (MQ=255)

                                     tGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGc                                                                                                            >  1:1217018/1‑148 (MQ=255)

                                        tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                                                         <  2:1833110/148‑1 (MQ=255)

                                         gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                        <  1:2453242/148‑1 (MQ=255)

                                              gaagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaa                                                                                                   <  2:2339926/148‑1 (MQ=255)

                                               aagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaaa                                                                                                  >  1:1988362/1‑148 (MQ=255)

                                                 gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                                                >  2:1059266/1‑148 (MQ=255)

                                                  cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGcc                                                                                               >  2:2870389/1‑148 (MQ=255)

                                                     ttCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgt                                                                                            >  2:2839729/1‑148 (MQ=255)

                                                     ttCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgt                                                                                            <  2:1430240/148‑1 (MQ=255)

                                                      tCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           <  1:865037/148‑1 (MQ=255)

                                                       cACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgttg                                                                                          <  2:1576323/148‑1 (MQ=255)

                                                         ccGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTtgtg                                                                                        >  1:2314885/1‑148 (MQ=255)

                                                             ttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCgg                                                                                    >  2:689424/1‑148 (MQ=255)

                                                             ttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCgg                                                                                    <  1:1281851/148‑1 (MQ=255)

                                                                 cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                >  2:3253218/1‑148 (MQ=255)

                                                                 cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                >  2:1699923/1‑148 (MQ=255)

                                                                  tCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                               <  1:2061000/148‑1 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  2:2509654/1‑148 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  2:2509819/1‑148 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  2:1888183/1‑148 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          <  1:3027487/148‑1 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  1:2168003/1‑148 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  1:504632/1‑148 (MQ=255)

                                                                       aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  1:1906528/1‑148 (MQ=255)

                                                                        ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  1:1043418/1‑148 (MQ=255)

                                                                        ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  1:1043505/1‑148 (MQ=255)

                                                                        ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  1:1043510/1‑148 (MQ=255)

                                                                        ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  1:1523453/1‑148 (MQ=255)

                                                                         cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                                                        <  1:3090188/148‑1 (MQ=255)

                                                                             aTGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGc                                                                    <  1:365218/148‑1 (MQ=255)

                                                                             aTGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGc                                                                    <  1:2965193/148‑1 (MQ=255)

                                                                              tGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGca                                                                   <  1:1332852/148‑1 (MQ=255)

                                                                                  ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAgg                                                               <  1:171983/148‑1 (MQ=255)

                                                                                     cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                            <  1:1307313/148‑1 (MQ=255)

                                                                                     cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                            <  1:82569/148‑1 (MQ=255)

                                                                                       aCGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcg                                                          >  2:814504/1‑148 (MQ=255)

                                                                                           tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                      >  2:2359903/1‑148 (MQ=255)

                                                                                                    tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                             <  2:1992498/148‑1 (MQ=255)

                                                                                                       ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                          >  2:1338381/1‑148 (MQ=255)

                                                                                                        cGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGACTGCACAGGCTGCGGCGCTGGCGCGACCtgt                                         >  2:1553636/1‑148 (MQ=255)

                                                                                                          cttcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTgg                                       >  2:2352682/1‑148 (MQ=255)

                                                                                                              tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaa                                   <  2:2014504/148‑1 (MQ=255)

                                                                                                               ttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa                                  <  2:846020/148‑1 (MQ=255)

                                                                                                               ttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa                                  <  2:846038/148‑1 (MQ=255)

                                                                                                               ttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa                                  <  2:1865215/148‑1 (MQ=255)

                                                                                                                  cgcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCAGGAAAGCCTGTTGTGCCGGATGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTg                               >  1:425266/1‑148 (MQ=255)

                                                                                                                   gcTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTgg                              <  1:2205347/148‑1 (MQ=255)

                                                                                                                   gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTgg                              <  2:74382/148‑1 (MQ=255)

                                                                                                                    ctgcGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                             >  2:990680/4‑148 (MQ=255)

                                                                                                                    cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                             <  1:366302/148‑1 (MQ=255)

                                                                                                                    cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                             >  1:2542959/1‑148 (MQ=255)

                                                                                                                     ttCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                            >  1:274804/1‑148 (MQ=255)

                                                                                                                      tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:3244862/1‑148 (MQ=255)

                                                                                                                      tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:2123040/1‑148 (MQ=255)

                                                                                                                      tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCCGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           <  1:1709841/148‑1 (MQ=255)

                                                                                                                      tCGGTTTATCCATAACTGGAGCAGGTTCAGATACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:1721736/1‑148 (MQ=255)

                                                                                                                         gTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        >  2:2201854/1‑148 (MQ=255)

                                                                                                                         gTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        >  1:79250/1‑148 (MQ=255)

                                                                                                                         gTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        >  1:3302669/1‑148 (MQ=255)

                                                                                                                          tttATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                       >  2:1839317/1‑148 (MQ=255)

                                                                                                                            tATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCaa                     <  1:2796179/148‑1 (MQ=255)

                                                                                                                                 aTAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCt                >  1:1855349/1‑148 (MQ=255)

                                                                                                                                  tAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTg               >  1:2761478/1‑148 (MQ=255)

                                                                                                                                   aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGt              >  1:184325/1‑148 (MQ=255)

                                                                                                                                         aGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGc        <  1:1058858/148‑1 (MQ=255)

                                                                                                                                           cAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTg      >  1:1905069/1‑148 (MQ=255)

                                                                                                                                           cAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTg      >  1:236130/1‑148 (MQ=255)

                                                                                                                                             ggTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCg    >  1:139669/1‑148 (MQ=255)

                                                                                                                                              gTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg   >  2:2722593/1‑148 (MQ=255)

                                                                                                                                               ttCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGc  <  1:3173894/148‑1 (MQ=255)

                                                                                                                                               ttCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGc  <  1:2693528/148‑1 (MQ=255)

                                                                                                                                               ttCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGc  <  1:1290016/148‑1 (MQ=255)

ATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523910‑2524200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`