Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,775 G→T 9.4% I196I (ATC→ATA)  eutQ ← RmlC‑like cupin domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,7750GT 9.4% 675.3 / 30.4 191I196I (ATC→ATA) eutQRmlC‑like cupin domain protein
Reads supporting (aligned to +/- strand):  ref base G (100/73);  new base T (13/5);  total (113/78)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 193 total aligned reads displayed.

TCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGA  >  CP009273/2567628‑2567922
                                                                                                                                                   |                                                                                                                                                   
  gCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGAt                                                                                                                                                   <  2:2343582/148‑1 (MQ=255)
  gCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGAt                                                                                                                                                   <  2:2342348/148‑1 (MQ=255)
   ctctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATc                                                                                                                                                  <  2:895467/148‑1 (MQ=255)
   ctctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATc                                                                                                                                                  <  2:728062/148‑1 (MQ=255)
   ctctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATc                                                                                                                                                  >  2:2957673/1‑148 (MQ=255)
   ctctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATc                                                                                                                                                  <  1:1994706/148‑1 (MQ=255)
   ctctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATc                                                                                                                                                  <  1:187443/148‑1 (MQ=255)
    tctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCa                                                                                                                                                 >  2:1799726/1‑148 (MQ=255)
     ctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCAt                                                                                                                                                >  2:1371618/1‑148 (MQ=255)
     ctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCAt                                                                                                                                                <  2:1421610/148‑1 (MQ=255)
        aGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGt                                                                                                                                             <  1:1607887/148‑1 (MQ=255)
          ccATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGttt                                                                                                                                           >  2:1774366/1‑148 (MQ=255)
            aTGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTgg                                                                                                                                         >  1:626801/1‑148 (MQ=255)
             tGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGc                                                                                                                                        >  2:3312749/1‑148 (MQ=255)
               cTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCt                                                                                                                                      >  2:2373726/1‑148 (MQ=255)
                 tCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTc                                                                                                                                    >  2:42759/1‑148 (MQ=255)
                      gatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGc                                                                                                                               >  1:1985238/1‑148 (MQ=255)
                      gatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGc                                                                                                                               >  1:2845893/1‑148 (MQ=255)
                      gatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGc                                                                                                                               <  1:605838/148‑1 (MQ=255)
                       atgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCg                                                                                                                              >  1:1229707/1‑148 (MQ=255)
                       atgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCg                                                                                                                              >  1:2076918/1‑148 (MQ=255)
                           aaTCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACa                                                                                                                          <  1:198324/148‑1 (MQ=255)
                               tttCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCa                                                                                                                      >  1:3254329/1‑148 (MQ=255)
                                ttCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAg                                                                                                                     >  2:1662694/1‑148 (MQ=255)
                                   aTAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTc                                                                                                                  <  2:222446/148‑1 (MQ=255)
                                   aTAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTc                                                                                                                  >  2:616344/1‑148 (MQ=255)
                                   aTAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTc                                                                                                                  <  1:2061516/148‑1 (MQ=255)
                                     aGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTccc                                                                                                                <  2:2514548/148‑1 (MQ=255)
                                      gggATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTcccc                                                                                                               >  1:3049084/1‑148 (MQ=255)
                                         aTTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTc                                                                                                            >  2:1583985/1‑148 (MQ=255)
                                         aTTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTc                                                                                                            >  2:3223776/1‑148 (MQ=255)
                                          ttGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTcc                                                                                                           >  2:627349/1‑148 (MQ=255)
                                          ttGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTcc                                                                                                           >  2:182015/1‑148 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                    >  2:1144492/1‑148 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                    >  1:955652/1‑148 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                    >  1:2829313/1‑148 (MQ=255)
                                                  tAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCAccct                                                                                                   >  2:2285320/1‑146 (MQ=255)
                                                   aGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATg                                                                                                  <  1:493140/148‑1 (MQ=255)
                                                    gCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGt                                                                                                 >  1:2632026/1‑148 (MQ=255)
                                                     ccGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTc                                                                                                >  1:881381/1‑148 (MQ=255)
                                                     ccGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTc                                                                                                >  1:2536498/1‑148 (MQ=255)
                                                          cAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGAttt                                                                                           <  1:2776782/148‑1 (MQ=255)
                                                            ggCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcg                                                                                         >  1:3064646/1‑148 (MQ=255)
                                                             gCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcgt                                                                                        >  1:239203/1‑148 (MQ=255)
                                                                aCATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTcgtcgt                                                                                     >  1:2186309/1‑148 (MQ=255)
                                                                 cATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTa                                                                                    >  2:2116755/1‑148 (MQ=255)
                                                                    aCAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGtt                                                                                 >  1:323845/1‑148 (MQ=255)
                                                                     cAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTc                                                                                <  2:2654033/148‑1 (MQ=255)
                                                                      aGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCa                                                                               >  1:1348277/1‑148 (MQ=255)
                                                                       ggAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAg                                                                              <  2:1533870/148‑1 (MQ=255)
                                                                           tttCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTc                                                                          >  2:730943/1‑148 (MQ=255)
                                                                           tttCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTc                                                                          <  1:1240759/148‑1 (MQ=255)
                                                                             tCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCa                                                                        <  1:2463246/148‑1 (MQ=255)
                                                                             tCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCa                                                                        <  1:573710/148‑1 (MQ=255)
                                                                                    ggATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGAGTTCGTCGTAGTTCAGGGTCCACGGgaag                                                                 >  1:394266/1‑148 (MQ=255)
                                                                                     gATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGgaaga                                                                >  2:3093599/1‑148 (MQ=255)
                                                                                         tCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGc                                                            >  1:1398876/1‑148 (MQ=255)
                                                                                          cGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCg                                                           <  1:2957673/148‑1 (MQ=255)
                                                                                          cGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCg                                                           <  1:2675076/148‑1 (MQ=255)
                                                                                               cAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTtctc                                                      <  2:120477/148‑1 (MQ=255)
                                                                                                aaaTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTcc                                                     >  2:3221581/1‑148 (MQ=255)
                                                                                                aaaTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTcc                                                     <  1:730351/148‑1 (MQ=255)
                                                                                                  aTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCa                                                   >  2:840671/1‑148 (MQ=255)
                                                                                                    tCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGACTGCGTTCTCCCACt                                                 >  2:3056885/1‑148 (MQ=255)
                                                                                                    tCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACt                                                 >  2:699596/1‑148 (MQ=255)
                                                                                                    tCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACt                                                 >  2:480143/1‑148 (MQ=255)
                                                                                                           tGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATaaa                                          <  2:2874188/148‑1 (MQ=255)
                                                                                                           tGGAGCCGTTCGGGATAAAAATCACGTCGCCTGCTTTGGCGATCATGGTTTTGCCTTCGTGGCGATCATGCTGCTCCCCTTACAGCACCATGTATATTTCGTCGTAGTTCAGTGTCCACGGGAAGAATGCGTTCTCCCACTGCATaca                                          <  2:589551/148‑3 (MQ=255)
                                                                                                            ggAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATaaaa                                         <  1:1356120/148‑1 (MQ=255)
                                                                                                             gAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAAc                                        <  2:1616574/148‑1 (MQ=255)
                                                                                                             gAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAAc                                        <  1:1963351/148‑1 (MQ=255)
                                                                                                               gCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcg                                      >  1:1782464/1‑148 (MQ=255)
                                                                                                                ccTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcgg                                     >  2:3069446/1‑148 (MQ=255)
                                                                                                                  tttCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcggcg                                   >  1:544268/1‑148 (MQ=255)
                                                                                                                  tttCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcggcg                                   >  2:1456743/1‑148 (MQ=255)
                                                                                                                  tttCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcggcg                                   >  2:1633093/1‑148 (MQ=255)
                                                                                                                        gATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtg                             <  2:711288/148‑1 (MQ=255)
                                                                                                                         aTAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgc                            >  2:195495/1‑148 (MQ=255)
                                                                                                                           aaaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctg                          <  1:2000007/148‑1 (MQ=255)
                                                                                                                           aaaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctg                          >  1:2047491/1‑148 (MQ=255)
                                                                                                                            aaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctgc                         >  2:888831/1‑148 (MQ=255)
                                                                                                                                 cACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcg                    >  1:1514621/1‑148 (MQ=255)
                                                                                                                                 cACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcg                    <  2:2290422/148‑1 (MQ=255)
                                                                                                                                  aCGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcgt                   <  2:2845893/148‑1 (MQ=255)
                                                                                                                                  aCGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcgg                   >  2:1663070/1‑147 (MQ=255)
                                                                                                                                   cGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcgtc                  >  2:1878427/1‑148 (MQ=255)
                                                                                                                                     tCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcgtcgt                <  2:2729957/148‑1 (MQ=255)
                                                                                                                                     tCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcgtcgt                >  1:2357174/1‑148 (MQ=255)
                                                                                                                                        ccTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGtctc             >  1:3267979/1‑148 (MQ=255)
                                                                                                                                         cTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTcc            >  2:2079113/1‑148 (MQ=255)
                                                                                                                                          tGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCg           >  1:2247878/1‑148 (MQ=255)
                                                                                                                                          tGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCg           >  2:1935684/1‑148 (MQ=255)
                                                                                                                                          tGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCg           <  1:1314692/148‑1 (MQ=255)
                                                                                                                                           gCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCgg          >  1:1007250/1‑148 (MQ=255)
                                                                                                                                           gCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCgg          >  1:1532156/1‑148 (MQ=255)
                                                                                                                                           gCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCgg          >  1:1532512/1‑148 (MQ=255)
                                                                                                                                            cTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGt         >  1:2361359/1‑148 (MQ=255)
                                                                                                                                            cTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGt         >  2:2743604/1‑148 (MQ=255)
                                                                                                                                            cTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGt         >  2:2034240/1‑148 (MQ=255)
                                                                                                                                                   gATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGa  <  2:2503704/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGA  >  CP009273/2567628‑2567922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑