Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,579,561 G→T 89.9% G158C (GGC→TGC)  narQ → sensory histidine kinase in two‑component regulatory system with NarP (NarL)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,579,5610GT89.9% 526.8 / 53.5 205G158C (GGC→TGC) narQsensory histidine kinase in two‑component regulatory system with NarP (NarL)
Reads supporting (aligned to +/- strand):  ref base G (13/8);  new base T (115/69);  total (128/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGA  >  CP009273/2579416‑2579705
                                                                                                                                                 |                                                                                                                                                
gAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTggctgc                                                                                                                                                <  1:1962343/148‑1 (MQ=255)
gAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTggctgc                                                                                                                                                <  1:1590349/148‑1 (MQ=255)
  ataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCAt                                                                                                                                              <  2:2664419/148‑1 (MQ=255)
   taatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATc                                                                                                                                             >  2:262522/1‑148 (MQ=255)
    aatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCg                                                                                                                                            <  1:2732652/148‑1 (MQ=255)
    aatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCg                                                                                                                                            >  2:2156285/1‑148 (MQ=255)
     atCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGATGGTGGTGGCGATTTCCCTGGCTGGCTGCATCgg                                                                                                                                           >  2:577093/1‑148 (MQ=255)
          cTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAttt                                                                                                                                      <  1:3062468/148‑1 (MQ=255)
             aGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCa                                                                                                                                   >  1:672221/1‑148 (MQ=255)
             aGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCa                                                                                                                                   >  1:1864933/1‑148 (MQ=255)
              gCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCAc                                                                                                                                  >  1:592323/1‑148 (MQ=255)
              gCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCAc                                                                                                                                  >  1:315280/1‑148 (MQ=255)
                  gggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTg                                                                                                                              >  2:2991007/1‑148 (MQ=255)
                     cGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCCGTATTTTCACGCTGGTc                                                                                                                           <  2:1697368/148‑1 (MQ=255)
                       aTTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCtt                                                                                                                         >  1:2826381/1‑148 (MQ=255)
                         ttGCCGTGGTATCCGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCtttt                                                                                                                       >  1:1505976/1‑148 (MQ=255)
                         ttGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCtttt                                                                                                                       >  2:1698333/1‑148 (MQ=255)
                         ttGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCtttt                                                                                                                       >  1:1056263/1‑148 (MQ=255)
                            ccGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTa                                                                                                                    <  1:806463/148‑1 (MQ=255)
                            ccGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTa                                                                                                                    <  2:1260197/148‑1 (MQ=255)
                              gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACt                                                                                                                  <  2:853536/148‑1 (MQ=255)
                              gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACt                                                                                                                  <  1:2608065/148‑1 (MQ=255)
                              gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACt                                                                                                                  <  1:2608253/148‑1 (MQ=255)
                              gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACt                                                                                                                  <  2:2509676/148‑1 (MQ=255)
                                     cAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgcc                                                                                                           >  1:700854/1‑148 (MQ=255)
                                     cAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgcc                                                                                                           >  1:700829/1‑148 (MQ=255)
                                      aGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGACTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgccg                                                                                                          >  2:1837660/1‑148 (MQ=255)
                                       ggCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgccgc                                                                                                         >  1:2417582/1‑148 (MQ=255)
                                        gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCa                                                                                                        >  1:1054873/1‑148 (MQ=255)
                                          cAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATa                                                                                                      <  2:1630365/148‑1 (MQ=255)
                                            atatTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                    >  2:1887743/1‑148 (MQ=255)
                                            atatTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                    >  1:1395100/1‑148 (MQ=255)
                                            atatTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                    >  2:2461427/1‑148 (MQ=255)
                                              atTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGcc                                                                                                  <  1:1287412/148‑1 (MQ=255)
                                               ttAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCa                                                                                                 >  2:1439616/1‑148 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  2:950570/1‑148 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  1:154231/1‑148 (MQ=255)
                                                 aataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATc                                                                                               >  1:481472/1‑148 (MQ=255)
                                                   taatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAg                                                                                             >  2:3102046/1‑148 (MQ=255)
                                                     atTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggt                                                                                           >  2:1879336/1‑148 (MQ=255)
                                                     atTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggt                                                                                           <  1:1395943/148‑1 (MQ=255)
                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                          <  2:187637/148‑1 (MQ=255)
                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                          <  2:268777/148‑1 (MQ=255)
                                                       tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtgg                                                                                         <  2:889332/148‑1 (MQ=255)
                                                       tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtgg                                                                                         >  1:2254252/1‑148 (MQ=255)
                                                         tGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGtt                                                                                       >  2:1895582/1‑148 (MQ=255)
                                                              aTCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcccc                                                                                  >  2:2746794/1‑148 (MQ=255)
                                                              aTCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcccc                                                                                  >  2:2337074/1‑148 (MQ=255)
                                                               tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCg                                                                                 <  2:1169894/148‑1 (MQ=255)
                                                                cAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                >  1:1625247/1‑148 (MQ=255)
                                                                   aTAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGa                                                                             <  1:2751420/148‑1 (MQ=255)
                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                           >  1:1881315/1‑148 (MQ=255)
                                                                      gACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATAAGCCATCAGGTGGTTGCCCCGCTGAATc                                                                          >  1:200431/1‑148 (MQ=255)
                                                                        ccTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAg                                                                        >  1:2269725/1‑148 (MQ=255)
                                                                        ccTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAg                                                                        >  1:1833690/1‑148 (MQ=255)
                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                    >  2:303699/1‑148 (MQ=255)
                                                                             tCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                   >  2:3002444/1‑148 (MQ=255)
                                                                             tCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                   <  1:377866/148‑1 (MQ=255)
                                                                                 acaggCTTTAAAGCAATACGCTGAACGAAAAATCCTGCATGTGGTGGCGATTTCCCTGTATTGATGCATCGGTATTTTCACGCTGGTCTATTTTACTTTGAGACTCATACTCCAACACGTGTTTGCCCCGCTGAATCAGCTCGTTaac                                                               >  2:3056922/4‑146 (MQ=255)
                                                                                   tGGCTTTCCAGCACTACGCTGAACGCAGAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                             <  2:2307626/148‑1 (MQ=255)
                                                                                   tGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                             <  1:439630/148‑1 (MQ=255)
                                                                                     gCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCa                                                           >  1:2440609/1‑148 (MQ=255)
                                                                                     gCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCa                                                           >  1:156698/1‑148 (MQ=255)
                                                                                           cAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGc                                                     >  1:2506523/1‑148 (MQ=255)
                                                                                             gCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGt                                                   >  2:1988365/1‑148 (MQ=255)
                                                                                              cACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTa                                                  <  1:1225024/148‑1 (MQ=255)
                                                                                               aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAt                                                 <  1:2908159/148‑1 (MQ=255)
                                                                                               aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAt                                                 <  1:2908043/148‑1 (MQ=255)
                                                                                               aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAt                                                 <  2:1395149/148‑1 (MQ=255)
                                                                                                  aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                              <  1:777823/148‑1 (MQ=255)
                                                                                                  aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                              <  2:700854/148‑1 (MQ=255)
                                                                                                  aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                              <  2:700829/148‑1 (MQ=255)
                                                                                                   cGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGaa                                             >  2:521350/1‑148 (MQ=255)
                                                                                                   cGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGaa                                             >  2:2844721/1‑148 (MQ=255)
                                                                                                   cGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGaa                                             <  1:968985/148‑1 (MQ=255)
                                                                                                     cTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAaca                                           >  2:1472101/1‑148 (MQ=255)
                                                                                                     cTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAaca                                           >  2:1471952/1‑148 (MQ=255)
                                                                                                       gAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACg                                         >  2:1371755/1‑148 (MQ=255)
                                                                                                        aaCGCAAAATGCTGCGGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACgg                                        <  2:2014430/148‑1 (MQ=255)
                                                                                                          cGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGc                                      <  2:752651/148‑1 (MQ=255)
                                                                                                            cAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAg                                    <  1:2428744/148‑1 (MQ=255)
                                                                                                            cAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAg                                    <  2:1787662/148‑1 (MQ=255)
                                                                                                            cAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAg                                    >  1:1340818/1‑148 (MQ=255)
                                                                                                              aaaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGtt                                  >  2:840515/1‑148 (MQ=255)
                                                                                                               aaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTc                                 >  1:2387166/1‑148 (MQ=255)
                                                                                                                     gctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcg                           >  1:576057/1‑148 (MQ=255)
                                                                                                                        ggtggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccg                        >  2:668923/1‑148 (MQ=255)
                                                                                                                          tggtggCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgcc                      >  1:1263684/1‑148 (MQ=255)
                                                                                                                           ggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                     <  2:3306791/148‑1 (MQ=255)
                                                                                                                           ggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                     <  1:1766456/148‑1 (MQ=255)
                                                                                                                           ggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                     <  2:1667620/148‑1 (MQ=255)
                                                                                                                             tggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCt                   >  2:116662/1‑148 (MQ=255)
                                                                                                                               gCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTgg                 <  1:3294546/148‑1 (MQ=255)
                                                                                                                                cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGa                >  2:1132605/1‑148 (MQ=255)
                                                                                                                                  aTTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATa              >  1:2628482/1‑148 (MQ=255)
                                                                                                                                  aTTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATa              >  2:2582084/1‑148 (MQ=255)
                                                                                                                                       ccTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAc         <  1:3192455/148‑1 (MQ=255)
                                                                                                                                        ctggctggCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCTGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAcc        <  1:3102046/148‑1 (MQ=255)
                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc     >  2:2888923/1‑148 (MQ=255)
                                                                                                                                              ggctgcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGa  >  2:1971660/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGA  >  CP009273/2579416‑2579705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑